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| 1 | Reduction of the incidence and mortality of rectal cancer bypolypectomy:a prospective cohort study in Haining County显示文摘AIM: To reduce the incidence and morlality of rectal cancerand address the hypothesis that colorectal cancer often arisefrom precursor lesion (s), either adenomas or non-alenomatous polyps, by conducting a population-basedmass screening for colorectal cancer in Haining County,Zhejiang, PRCMETHODS: From 1977 to 1980, physicians screened thepopulation of Haining County using 15 cm rigid endoscopy.Of over 240 000 participants, 4076 of them were diagnoselwith Precursor Iesions, eitler alenomas or non-adenomatous polyps, which were then removedl surgically.All individuals with precursor lesionswere followed up andreexamined by endoscopy every two to five years upto 1998.RESULTS: After the initial screening, 953 metactronousadenomas and 417 non-adenomatous polyps ware detected andremoved from the members of this cohort. Further, 27 cases ofcolorectal cancer were detected and treated. Log-rank tuestsshowed that the survival time among those cancer patients whounderwent mass screening increased significantly comparedtothat of other colorectal cancer patients (P < 0.0001). Accordingto the population-based cancer registry in Haining County,age-adjusted incidence and mortality of rectal cancer decreasedby 41% and 29 % from 1977-1981 to 1992-1996, respectively.Observed cumulative 20-year rectal cancer incidence was 31%lower than the expected in the screened group; the mortalitydue to rectal cancer was 18 % lower thah the expected in thescreened group.CONCLUSION: Mass screening for rectal cancer andprecursor lesions with protocoscopy in the generalpopulation and periodical following-up with routineendoscopy for high-risk patients may decrease both theincidence and mortality of rectal cancer. | Shu Zheng Xi-Yong Liu Qi Dong,Cancer Institute,Zhejiang University,88 Jiefang Road,HangZhou 310009,Zhejiang Province,China Ke-feng Ding Lin-Bo Wang Pei-Lin Qiu Su-Zhan Zhang,The 2~(nd)affiliated Hospital,Medical School of Zhejiang University,88 Jiefang Road,HangZhou 310009,Zhejiang Province,China Xin-Feng Ding Yong-Zhou Shen Gao-Fei Shen Oi-Rong Sun Wei-Dong Li,Haining Cancer Institute,Haining 314400,Zhejiang Province,China | 2002 | World Journal of Gastroenterology2002，8，3： | 37 |
| 2 | Clinical significance of vascular endothelial growth factor expression and neovascularization in colorectal carcinoma显示文摘AIM: To clarify the association of vascular endothelial growth factor (VEGF) and microvascular density (MVD) expression with the angiogenesis and prognosis of colorectal cancer.METHODS: A total of 97 cases of colorectal cardnomas were examined by immunohistochemical staining (SP method), using anti-VEGF and anti-factor CD34^+ monodonal antibodies,RESULTS: VEGF positive staining was obtained in 68 out of 97 cases (70.1%), and observed mainly in the cytoplasm of tumor cells, and also frequently in stromal cells. VEGF expression was more intense in poorly differentiated adenocarcinoma in comparison with others, but there was no significant correlation between VEGF expression and age,sex and stage. A significant correlation was found between the MVD and grades, and there was no significant relationship between the MVD and age, sex, and stage. The MVD in the VEGF positive group (68 cases) was higher than that in the negative group. Upon multivariate analysis, the significant variables were stage, tumor grade and MVD; VEGF expression was not an independent prognostic factor.CONCLUSION: The expression of VEGF has a significant correlation with MVD; MVD expression has prognostic value but VEGF has not in colon cancer. | Shu Zheng Ming-Yong Han Zuo-Xiang Xiao Jia-Ping Peng Qi Dong Cancer Institute,Zhejiang University,Hangzhou 310009,Zhejiang Province,China | 2003 | World Journal of Gastroenterology2003，9，6： | 38 |
| 3 | Diagnosis and treatment of congenital choledochal cyst: 20 years' experience in China显示文摘AIM To summarize the experience of diagnosis and treatment of congenital choledochal cyst in the past 20years ( 1980 2000).``METHODS The clinical data of 108 patients admitted from 1980 to 2000 were analyzed retrospectively.RESULTS Abdominal pain, jaundice and abdominal mass were presented in most child cases. Clinical symptoms in adult cases were non-specific, resulting in delayed diagnosis frequently. Fifty-seven patients (52.7%) had coexistent pancreatiobiliary disease. Carcinoma of the biliary duct occurred in 18 patients (16.6%). Ultrasonic examination was undertaken in 94 cases, ERCP performed in 46 cases and CT in 71 cases. All of the cases were correctly diagnosed before operation. Abnormal pancreatobiliary duct junction was found in .39 patients.Before 1985 the diagnosis and classification of congenital choledochal cyst were established by ultrasonography preoperatively and confirmed during operation, the main procedures were internal drainage by cyst enterostomy.After 1985, the diagnosis was established by ERCP and CT. and cystectomy with Roux-en-Y hepaticojejunostomy was the conventional procedures. In 1994, we reported a new and simplified operative procedure in order to reduce the risk of choledochal cyst malignancy. Postoperative complication was mainly retrograde infection of biliary tract, which could be controlled by the administration of antibiotics, there was no perioperative mortality.``CONCLUSION The concept in diagnosis and treatment of congenital choledochal cyst has obviously been changed greatly. CT and ERCP were of great help in the classification of the disease. Currently, cystectomy with Roux-en-Y hepaticojejunostomy is strongly recommended as the choice for patients with type I and type Ⅳ cysts.Piggyback orthotopic liver transplantation is indicated in type \ cysts (Carolis disease) with frequently recurrent cholangitis. | Liu-Bin Shi~1 Shu-You Peng~1 Xing-Kai Meng~1 Cheng-Hong Peng~1 Ying-Bin Liu~1 Xiao-Peng Chen~1 Zhen-Ling Ji~2 De-Tong Yang~2 Huai-Ren Chen~2 1 Department of Surgery,The Second Affiliated hospital of Zhejiang University School of Medicine,Hangzhou 310009,China2 Department of Surgery,Affiliated Zhongda Hospital of Southeast University,Nanjing 210009,China | 2001 | World Journal of Gastroenterology2001，7，5： | 18 |
| 4 | Management of traumatic hemothorax by closed thoracic drainage using a central venous catheter显示文摘Objective:To evaluate the efficacy and safety of the treatment of traumatic hemothorax by closed pleural drainage using a central venous catheter(CVC),compared with using a conventional chest tube.Methods:A prospective controlled study with the Ethics Committee approval was undertaken.A total of 407 patients with traumatic hemothorax were involved and they were randomly assigned to undergo closed pleural drainage with CVCs(n=214) or conventional chest tubes(n=193).The Seldinger technique was used for drainage by CVC,and the conventional technique for drainage by chest tube.If the residual volume of the hemothorax was less than 200 ml after the daily volume of drainage decreased to below 100 ml for two consecutive days,the treatment was considered successful.The correlative data of efficacy and safety between the two groups were analyzed using t or chi-squared tests with SPSS 13.0.A P value of less than 0.05 was taken as indicating statistical significance.Results:Compared with the chest tube group,the operation time,fraction of analgesic treatment,time of surgical wound healing,and infection rate of surgical wounds were significantly decreased(P<0.05) in the CVC group.There were no significant differences between the two groups in the success rate of treatment and the incidence of serious complications(P>0.05),or in the mean catheter/tube indwelling time and mean medical costs of patients treated successfully(P>0.05).Conclusions:Management of medium or large traumatic hemothoraxes by closed thoracic drainage using CVC is minimally invasive and as effective as using a conventional large-bore chest tube.Its complications can be prevented and it has the potential to replace the large-bore chest tube. | Jian-hua YI1,Hua-bo LIU2,Mao ZHANG1,Jun-song WU1,Jian-xin YANG1,Jin-ming CHEN1,Shan-xiang XU1,Jian-an WANG1(1Department of Emergency,the Second Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310009,China)(2Department of Emergency,Zhoushan Hospital of Zhejiang Province,Zhoushan 316004,China) | 2012 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2012，13，1： | 18 |
| 5 | Detection of H.pylori DNA in gastric epithelial cells by in situ hybridization显示文摘AIM: To investigate the presence of H. pylori DNA withingastric epithelial cells in patients with H. pylori infection andits possible carcinogenic mechanism.METHODS: Total 112 patients, with pathologically confirmedchronic superficial gastritis, chronic atrophic gastritis,intestinal metaplasia, atypical hyperplasia or gastrio cancerwere studied .Among them, 28 were H. pylori negative and84 H. pylori positive. H. pylori DNA in gastric epithelialcells was detected by GenPoint catalyzed signalamplification system for in situ hybridization.RESULTS: In the H. pylori positive group, zero out of 24chronic superficial gastritis (0. 0 %), four out of 25precancerous changes (16.0 %) and thirteen out of 35gastric cancers (37. 1 %) showed H. pylori DNA in thenucleus of gastric epithelial cells, the positive rates of H.pylori DNA in the nucleus of gastric epithelial cells wereprogressively inoreased in chronic superficial gastritis,precancerous changes and gastric cancer groups (χ2 = 12.56, P = 0. 002); One out of 24 ohronic superficial gastritis(4.2 %), eleven out of 25 precancerous ohangas (44.0 %)and thirteen out of 35 gastric cancers (37. 1 %) showed H.pylori DNA in the cytoplasm of gastric epithelial cells (χ2 =10.86, P = 0.004). In the H. pylorinegative group, only onepatient with gastric cancer was found H. pylori DNA in thenucleus of gastric epithelial cells; Only two patients, onepatient with precancerous changes and another with gastriccancer, showed H. pylori DNA in the cytoplasm of gastricepithelial calls. Furthermore, H. pylori DNA must have been inthe ayteplasm as long as it existed in the nucleus of gastricepithelial cells.CONCLUSION: H. pyloriDNA exists both in the nucleus andthe cytoplasm of gastric epithelial cells in patients with H.pylori infections. The pathological progression from chronicsuperficial gastritis, precancerous changes to gastric canceris associated with higher positive rates of H. pylori DNApresence in the nucleus of gastric epithelial cells. | Xin-Liang Lu Ke-Da Oian Xun-Qiu Tang Yong-Liang Zhu Qin Du,Department of Digestive Diseases,Second Affiliated Hospital,Zhejiang University Medical College,Hangzhou 310009,Zhejiang Province,China | 2002 | World Journal of Gastroenterology2002，8，2： | 11 |
| 6 | High frequencies of HGV and TTV infections in blood donors in Hangzhou显示文摘ALM To determine the frequencies of HGV and TTVinfections in blood donors in Hangzhou.METHODS RT-nested PCR for HGV RNA detection and semi-nested PCR for TTV DNA detection in the sera from 203 blood donors, and nucleotide sequence analysis were performed.``RESULTS Thirty-two ( 15.8%) and 30 (14.8%) of the 203serum samples were positive for HGV RNA and TTV DNA,respectively. And 5 (2.5%) of the 203 serum samples were detectable for both HGV RNA and TTV DNA.Homology of the nucleotide sequences of HGV RT-nested PCR products and TTV semi-nested PCR products from 3serum samples compared with the reported HGV and TTV sequences was 89.36%. 87.94%, 88.65% and 63.51%.65.77% and 67.12%. respectively.``CONCLUSION The infection rates of HGV and/or TTV inblood donors are relatively high. and to establish HGV and TTV examinations to screen blood donors is needed for transfusion security. The genomic heterogeneity of TTV or HGV is present in the isolates from different areas. | Jie Yan~1 Li-Li Chen~2 Yi-Hui Luo~1 Ya-Fei Mao~1 Meng He~1 1 Department of Pathogenic Biology,Medical School of Zhejiang University,Hangzhou 310006,Zhejiang Province,China2 Department of Stomatology,The Second Affiliated Hospital,Medical School of Zhejiang University,Hangzhou 310009,Zhejiang Province,China | 2001 | World Journal of Gastroenterology2001，7，5： | 11 |
| 7 | Oxidative Stress before and after Operation in Patients with Chronic Cholecystitis Containing Gallstone显示文摘To study the relationship of oxidative, antioxidative constituents and antioxidases in blood with chronic cholecystitis containing gallstone, levels of lipoperoxides (LPO), nitric oxide (NO), vitamin C(VC), vitamin E (VE) and β carotene (β CAR) in plasma as well as level of LPO, activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH Px) in erythrocytes were investigated by spectrophotometric assay in 107 patients with this condition (PCg) and 100 healthy volunteers (HVs). Compared with HVs group, the average value of LPO and NO in plasma and that of LPO in erythrocytes of PCg group were significantly increased ( P <0.0001), while that of VC, VE and β CAR in plasma and the average activities of SOD, CAT and GSH Px in erythrocytes were significantly decreased ( P <0.0001). Linear regression and correlation analysis for 107 preoperative PCg showed that the value of LPO and NO in plasma and that of LPO in erythrocytes of PCg gradually increased ( P <0.0001), representing a significant linear positive correlation. The value of VC, VE and β CAR in plasma and that of SOD, CAT and GSH Px in erythrocytes of PCg gradually decreased ( P <0.0001), representing a significant linear negative correlation. Stepwise regression and correlation analysis for 107 preoperative PCg suggested that the closest correlation was observed between the course of disease and the value of NO and VC in plasma and that of SOD, GSH Px and LPO in erythrocytes, r =0.7306, F =32.1408, P <0.0001. Compared with the preoperative PCg group, the average value of LPO and NO in plasma and that of LPO in erythrocytes of the postoperative PCg group were significantly decreased ( P <0.0001). Furthermore, the average value of VC in plasma and that of SOD, CAT and GSH Px in erythrocytes of the postoperative PCg group were significantly increased ( P <0.0001), whereas no significant difference was found between their average value of VE and β CAR in plasma. These findings suggested that oxidative stress was an aggravating pathological condition in PCg group. Therefore, we recommend that in treating PCg, antioxidants such as VC, VE, β CAR should be given in order to alleviate their potential oxidative damages. | ZHOU JUN FU , CHEN PENG , YANG JIN LU +, ZHU YOU GEN + PENG CHENG HONG , AND WU YU LIAN The 2nd Affiliated Hospital, Medical College of Zhejiang University, 88 Jiefang Road, Hangzhou, 310009, Zhejian | 2000 | Biomedical and Environmental Sciences2000，13，4： | 10 |
| 8 | Suppression of Type 1 Insulin-like Growth Factor Receptor Expression by Small Interfering RNA Inhibits A549 Human Lung Cancer Cell Invasion in vitro and Metastasis in Xenograft Nude Mice显示文摘癌症侵略和转移,包含许多病理学的过程和 cytophysiological 变化,贡献肺癌症的高死亡。类型 1insulin-like 生长因素受体(IGF-1R ) ,把前进和侵略与癌症联系了,是在肺癌症的一个潜在的反侵略和反转移目标。禁止肺癌症房间的侵略性质,我们在由小介入 RNA ( siRNA )技术的 A549 人的肺癌症房间的成功地下面调整的 IGF-1R 基因表达,并且评估了它的效果oninvasion相关的基因表达,在 vitro 侵略的肿瘤房间,并且在有为 IGF-1R 表示发卡 siRNA 的一个原生质标志的异种皮移植 nudemice.A549 房间 transfected 的转移在 mRNA 水平以及蛋白质 level.Inbiological 试金显示出一个显著地减少的 IGF-1R 表达式, transfected A549 房间显示出 cell-matrixadhesion ,移植和侵略的重要减小。与这些结果一致,我们发现那条下面规定大减小附随地在侵略相关的基因表达伴随的 ofIGR-1R , includingMMP-2 , MMP-9 , u-PA ,并且为 IGF-1R 的 plasmidexpressing 发卡 siRNA 的 IGF-1R 特定的下游的 p-Akt.Direct 尾巴静脉注射显著地在结果显示出的 nudemice.Our 禁止了肺转移的形成是的 siRNA 的治疗学的潜力为在禁止肺癌症侵略和转移的基因治疗的一个方法。 | Jianfang QIAN~1 Aiqiang DONG~1 Minjian KONG~1 Zhiyuan MA~2 Junqiang FAN~1 and Guanyu JIANG~3 ~1Department of Cardiothoracic Surgery,Second Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310009,China ~2Department of Cardiovascular Surgery,Shanghai Jiaotong University Affiliated First People’s Hospital,Shanghai 200080,China ~3Department of Emergency,Second Affiliated Hospital School of Medicine,Zhejiang University,Hangzhou 310009,China | 2007 | Acta Biochimica et Biophysica Sinica2007，39，2： | 9 |
| 9 | Quality of life in patients with liver cancer after operation:a 2-year follow-up study显示文摘BACKGROUND:Quality of life (QL) is a concept which reflects the physical, social, and emotional attitudes and behaviours of an individual. QL assessment is becoming in- creasingly recognised as an outcome and predictor for can- cer patients. Although hepatectomy has been widely ac- cepted as treatment of choice to offer a chance of cure for patients with liver cancer, little is known about the subjec- tive clinical results after this operation. This prospective study was designed to evaluate the Pre- and postoperative quality of life in patients with liver cancer. METHODS: The quality of life of 36 consecutive patients was measured using gastrointestinal quality of life index (GQLI) regularly 2 years after the operation, starting with a preoperative measurement. RESULTS: The score of mean preoperative GQLI was 106 ± 13 points, and it was reduced significantly 2-10 weeks after the operation (86-98) (P <0. 05-0.001). The quality of life recovered gradually. The GQLI score was 101 ± 21 points 4 months after operation and increased to the preo- perative level ( P > 0. 05 ). In the patients who survived more than 9 months, the GQLI score was higher than that before the operation. Major hepatectomy (lobectomy and combined segmentectomy) reduced the GQLI score more evidently than did minor hepatectomy (simple segmentec- tomy ) in 2-5 weeks after the operation (P <0.05). The age and preoperative liver function of the patients played an im- portant role in the recovery of the quality of life in the early postoperative stage (P <0.05). The patients with tumor re- currence showed a continuous decrease of the quality of life (P<0.05-0.001). CONCLUSIONS: The assessment of the quality of life is meaningful for patients with liver cancer. Tumor recur- rence, poor liver function and major operation are the most important factors for reducing the quality of life. He- patic resection is justified by its effects on the survival and the quality of life of the patients. | Li Chen, Yong Liu, Guo-Gang Li, Si-Feng Tao, Yuan Xu and Hua Tian Hangzhou, China : Department of Surgery, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009 , China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，4： | 9 |
| 10 | A ten-year study on non-surgical treatment of postoperative bile leakage显示文摘AIM: To summarize systematically our ten-year experiencein non-surgical treatment of postoperative bile leakage, andexplore its methods and indications.METHODS: The clinical data of 57 patients with postoperativebile leakage treated non-surgically from January 1991 toDecember 2000 were reviewed retrospectively.RESULTS: The site of the leakage was mainly the disruptedor damaged fistulous tracts oft tube in 25 patients (43.9 %),the fossae of gallbladder in 14 cases (24.6 %), the cut surfaceof liver in 7 cases(12.3 %), and it was undetectable in theother 2 cases. Besides bile leakage, the wrong ligation of bileducts was found in 3 patients, residual stones of the distal bileduct in 5 patients, benign papillary strictures in 3, and bilomaresulting from bile collections in 2. The diagnoses were madeaccording to the history of surgery, clinical situation, abdominalparacentesis, ultrasonography, ERCP, PTC, MRI/MRCP,gastroscopy and percutaneous fistulography. All 57 patientswere treated non-surgically at the beginning of bile leakage.The non-surgical methods included keeping original drainageunobstructed, percutaneous abdominal paracentesis ordrainage, percutaneous transhepatic cholangial/biliary drainage(PTCD/PTBD),endoscopic management, traditional Chinesemedicine and so on. Of the 57 patients,2 patients died,5 wereconverted to reoperation later, the other 50 were directly curedby non-surgical methods without any complication. The curerate of the non-surgery was 82.5 % (50/57).CONCLUSION: Many nonoperative methods are availableto treat postoperative bile leakage. Non-surgical treatmentmay serve as the first choice for the treatment of bile leakagefor its advantages in higher cure rate, convenience and safetyin practice. It is important to choose the specific non-surgicalmethod according to the volume, site of bile leakage andpatient′s condition. | Xiao-Peng Chen Shu-You Peng Cheng-Hong Peng Yin-Bi Liu Liu-Bin Shi Xian-Chuan Jiang Hong-Wei Shen Yuan-Liang Xu Department of Surgery,Second Affiliated Hospital,Medical School of Zhejiang University,Hangzhou 310009,Zhejiang Province,China Xiao-Peng Chen Shu-Bin Fang Jing Rui Xiang-Hou Xia Guo-Hai Zhao Department of Surgery,Affiliated Yijishan Hospital,Wannan Medical College,Wuhu 241001,Anhui Province,China | 2002 | World Journal of Gastroenterology2002，8，5： | 8 |
| 11 | Safe upper limit of intermittent hepatic inflow occlusion for liver resection in cirrhotic rats显示文摘AIM To evaluate the effects of varying ischemic durations on cirrhotic liver and to determine the safe upper limit of repeated intermittent hepatic inflow occlusion.``METHODS Hepatic ischemia in cirrhotic rats was induced by clamping the common pedicle of left and median lobes after non-ischemic lobes resection. The cirrhotic rats were divided into six groups according to the duration and form of vascular clamping: sham occlusion (SO),intermittent occlusion for 10 (IO-10), 15(IO-15). 20(IO-20)and 30(IO-30) minutes with 5 minutes of refiow andcontinuous occlusion for 60 minutes (CO-60). All animals received a total duration of 60 minutes of hepatic inflow occlusion. Liver viability was investigated in relation of hepatic adenylate energy charge ( EC ).Triphenyltetrazollum chloride (TTC) reduction activities were assayed to qualitatively evaluate the degree of irreversible hepatocellular injury. The biochemical and morphological changes were also assessed and a 7-day mortality was observed.``RESULTS At 60 minutes after reperfusion following atotal of 60 minutes of hepatic inflow occlusion, EC values in lO-L0 (0.749±:0.012) and IO-15 (0.699 ±0.002) groups were rapidly restored to that in SO group (0. 748± 0.016).TTC reduction activities remained in high levels (0. 144 ±0.002 mg/mg protein, 0. 139 + 0.003 mg/mg protein and 0.121 ± 0.003 mg/mg protein in SO, IO-10 and IO-15groups, respectively). But in IO-20 and IO-30 groups, EC levels were partly restored (0.457 ± 0.023 and 0.534 ±0.027) accompanying with a significantly decreased TTCreduction activities (0.070 ± 0.005 mg/mg protein and 0.061 ±0.003 rng/mg protein). No recovery in EC values , i).228 ± 0.004) and a progressive decrease in TTC reduction activities ( 0.03.3 ± 0.002 mg/mg protein) were shown in CO-60 group. Although not significantly different, the activities of the serum aspartate aminotransferase (AST) on the third postoperative day (POD3 ) and POD7 and of the serum alanineaminotransferase (ALT) on POD3 in CO-60 group remained higher than that in intermittent occlusion groups.Moreover, a 60% animal mortality rate and more severe morphological alterations were also shown in CO-60group.``CONCLUSION Hepatic inflow occlusion during 60 minutes for liver resection in cirrhotic rats resulted in less hepatocellular injury when occlusion was intermittent rather than continuous. Each period of 15 minutes was the safe upper limit of repeated intermittent vascular occlusion that the cirrhotic liver could tolerate without undergoing irreversible hepatocellular injury. | Dao-Xiong Lei~(1,2) Cheng-Hong Peng~1 Shu-You Peng~1 Xian-Chuan Jiang~1 Yu-Lian Wu~1 Hong-Wei Shen~1 1 Department of Surgery,Second Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310009,Zhejiang Province,China2 Department of Surgery,Zhongnan Hospital,Wuhan University School of Medicine,Wuhan 430071,Hubei Province,China | 2001 | World Journal of Gastroenterology2001，7，5： | 8 |
| 12 | Investigation of HGV and TTV infection in sera and saliva from non-hepatitis patients with oral diseases显示文摘AIM: To determine the frequencies of HGV and TTV infectionsin serum and saliva samples of non-hepatitis patients withoral diseases in Hangzhou area, and to understand thecorrelation between detected results of HGV RNA and/or TTVDNA in sera and in saliva from the same patientsMETHODS: RT-nested PCR for HGV RNA detection andsemi-nested PCR for nv DNA detection were performed inthe serum and saliva samples from 226 non-hepatitis patientswith oral diseases, and nucleotide sequence analysis.RESULTS: Twenty-seven (11.9 %) and 21 (9.3 %) of the226 serum samples were only positive for HGV RNA andlrv DNA, respectively. 10 (4.4 %) and 9 (3.9 %) of the226 saliva samples were only positive for HGV RNA andTTV DNA, respectively. And 7 (3.1%) of the serum samplesand 2 (0.9 %) of the saliva samples showed the positiveamplification results for both HGV RNA and Irv DNA. 12saliva samples from the 34 patients (35.3 %) with HGV orHGV/TTV viremia and 11 saliva samples from the 28 patients(39.3 %) with TTV or HGV/TTV viremia were HGV RNAdetectable, respectively, including two patients positive forboth HGV RNA and TTV DNA in serum and saliva samples.No saliva samples from the 226 patients were found to beHGV RNA or nv DNA detectable while their serum sampleswere negative for HGV or TTV. Homologies of the nucleotidesequences of HGV and TTV amplification products from theserum and saliva samples of the two patients comparedwith the reported sequences were 88.65-91.49 % and65.32-66.67 %, respectively. In comparison with thenucleotide sequences of amplification products betweenserum and from saliva sample from any one of the twopatients, the homologies were 98.58 % and 99.29 % forHGV, and were 98.65 % and 98.20 % for rTV, respectively.CONCLUSION: Relatively high carrying rates of HGV and/or TTV in the sera of non-hepatitis patients with oral diseasesin Hangzhou area are demonstrated. Parts of the carriersare HGV and/or TTV positive in their saliva. The results ofthis study indicate that dentists may be one of the populationswith high risk for HGV and/or TTV infection, and by way of saliva HGV and TTV may be transmitted among individuals. | Jie Yan Department of Pathogenic Biology,College of Medical Science,Zhejiang University,Hangzhou 310031,Zhejiang Province,China Li-Li Chen Department of Stomatology,The Second Affiliated Hospital,College of Medical Science,Zhejiang University,Hangzhou 310009,Zhejiang Province,China Yong-Liang Lou Xiao-Zhi Zhong Wenzhou Medical College,Wenzhou 325000,Zhejiang Province,China | 2002 | World Journal of Gastroenterology2002，8，5： | 6 |
| 13 | Case Report:Lance-Adams syndrome:a report of two cases显示文摘Chronic post-hypoxic myoclonus, also known as Lance-Adams syndrome (LAS), is a rare complication of successful cardiopulmanry resuscitation often accompanied by action myoclonus and cerebellar ataxia. It is seen in patients who have un-dergone a cardiorespiratory arrest, regained consciousness afterwards, and then developed myoclonus days or weeks after the event. Worldwide, 122 cases have been reported in the literature so far, including 1 case of Chinese. Here we report 2 Chinese LAS patients with detailed neuroimagings. Cranial single photon emission computed tomography (SPECT) of patient 1, a 52-year-old woman, showed a mild hypoperfusion in her left temporal lobe, whereas patient 2, a 54-year-old woman, manifested a mild bilateral decrease of glucose metabolism in the frontal lobes and a mild to moderate decrease of the N-acetyl aspartate (NAA) peak in the bilateral hippocampi by cranial [18F]-fluorodeoxyglucose positron emission tomographic (PET) scan and cranial magnetic resonance spectroscopy (MRS), respectively. We also review the literature on the neuroimaging, pathogenesis, and treatment of LAS. | ZHANG Yan-xing1,2, LIU Jian-ren1, JIANG biao3, LIU Hui-qin1, DING Mei-ping1, SONG Shui-jiang1, ZHANG Bao-rong1, ZHANG Hong4, XU Bin5, CHEN Huai-hong6, WANG Zhong-jin1, HUANG Jian-zheng1 (1Department of Neurology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China) (2Department of Neurology, Shaoxing People’s Hospital, Shaoxing 312000, China) (3Department of Radiology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China) (4Department of Nuclear Medicine, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China) (5Department of Neurology, the Second Affiliated Hospital, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310005, China) (6Department of Geratology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China) | 2007 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2007，8，10： | 6 |
| 14 | Activity of boanmycin against colorectal cancer显示文摘INTRODUCTIONBoanmycin (Bleomycin A6, BAM ), a newantitumor antibiotic, was isolated from manycomponents of bleomycin (BLM) produced bystreptomyces pingyangensis which were obtainedfrom a soil sample collected in Pingyang County,Zhejiang Province, China. Boanmycin has a similarchemical structure to that of BLM, but the terminalamine moiety is different[ 1]. | Yong Chuan Deng1 Yong Su Zhen2 Shu Zheng1 Yu Chuan Xue2 1Cancer Institute, Medical School, Zhejiang University, Hangzhou 310009, Zhejiang Province, China2Institute of Medicinal Biotechnology, CAMS & PUMC, Beijing 100050, China | 2001 | World Journal of Gastroenterology2001，7，1： | 5 |
| 15 | Analysis for phenotype of HNPCC in China显示文摘AIM: The aims of this study were to identify theclinicopathological features of Chinese HNPCC families andto evaluate the value of criteria for suspected HNPCC(sHNPCC) in clinical diagnosis.METHODS: According to the follow-up records, 54 HNPCCfamilies (including 12 TCG-HNPCC families and 42 sHNPCCfamilies) were screened out from patients with colorectal cancers(CRCs), operated upon in 2nd Affiliated Hospital of ZhejiangUniversity from 1984 to 2001. Clinical data of probands andtumor spectrum in these families were listed and analyzed,RESULTS: (1) Mean age, proportion of colonic cancer, poorlydifferentiated cancer, multiple CRCs and Dukes′ A+B of theprobands in ICG-HNPCC and sHNPCC kindred were 39ys and47.5ys, 75 % and 62 %, 0 and 12.8 %, 16.7 % and 14.3 %,58.3 % and 81%,respectively. Compared with sporadiccolorectal cancers, probands from ICG-HNPCC and sHNPCCfamilies were obviously different at age of onset(P=0.025and 0.031), tumor location (P=0.001 and 0.000),differentiation(P=0.002 and 0.011) and development ofmultiple tumors (P=0.014 and 0.002). (2) A total of 178malignant neoplasms were found in 54 HNPCC families,including 139 colorectal cancers. Besides of colorectal cancer,extracolonic tumors occurred in stomach, endometrium,hepatobiliary system, and so on (8 gastric cancers, 6 endometrialcancers, 6 hepatobiliary system cancers and 19 others) canalso be seen in Chinese ICG-HNPCC and sHNPCC families.CONCLUSION: (1) Chinese HNPCC families have specificclinicopathological features, such as early onset, predilectionfor the involvement of colon, tendency of multiple CRCs,development of extracolonic tumors and well differentiation.(2) The criteria for suspected HNPCC is useful in clinicaldiagnosis and management of HNPCC. | Yong-Mao Song Department of Oncology,2nd Affiliated Hospital,Medical College,Zhejiang University,Hangzhou 310009,Zhejiang Province,China Shu Zheng Cancer Institute,Zhejiang University,Hangzhou 310009,Zhejiang Province,China | 2002 | World Journal of Gastroenterology2002，8，5： | 5 |
| 16 | Continuous release of interleukin 12 from microencapsulated engineered cells for colon cancer therapy显示文摘AIM: To explore the anti-tumor immunity against CT26 colon tumor of the microencapsulated cells modified with murine interleukine-12 (mIL-12) gene.METHODS: Mouse fibroblasts (NIH3T3) were stably transfected to express mIL-12 using expression plasmids carrying mIL-12 gene (p35 and p40), and NIH3T3-mIL-12cells were encapsulated in alginate microcapsules for longterm delivery of mIL-12. mIL-12 released from the microencapsulated NIH3T3-mIL-12 cells was confirmed using ELISA assay. Transplantation of the microencapsulated NIH3T3-mIL-12 cells was performed in the tumor-bearing mice with CT26 cells. The anti-tumor responses and the anti-tumor activities of the microencapsulated NIH3T3-mIL12 cells were evaluated.RESULTS: Microencapsulated NIH3T3-mIL-12 cells could release mIL-12 continuously and stably for a long time. After the microencapsulated NIH3T3-mIL-12 cells were transplanted subcutaneously into the tumor-bearing mice for 21 d, the serum concentrations of mIL-12, mIL-2 and mIFN-γ the cytotoxicity of the CTL from the splenocytes and the NK activity in the treatment group were significantly higher than those in the controls. Moreover, mIL-12 released from the microencapsulated NIH3T3-mIL-12 cells resulted in a significant inhibition of tumor proliferation and a prolonged survival of tumor-bearing mice.CONCLUSION: The microencapsulated NIH3T3-mIL-12cells have a significant therapeutic effect on the experimental colon tumor by activating anti-tumor immune responses in vivo. Microencapsulated and genetically engineered cells may be an extremely versatile tool for tumor gene therapy. | Shu Zheng Zuo-Xiang Xiao Yue-Long Pan Ming-Yong Han Qi Dong Cancer Institute,Zhejiang University,Hangzhou 310009,Zhejiang Province,China | 2003 | World Journal of Gastroenterology2003，9，5： | 4 |
| 17 | What we know about ST13, a co-factor of heat shock protein, or a tumor suppressor?显示文摘This article is to summarize the molecular and functional analysis of the gene “suppression of tumorigenicity 13” (ST13). ST13 is in fact the gene encoding Hsp70 interacting protein (Hip), a co-factor (co-chaperone) of the 70-kDa heat shock proteins (Hsc/Hsp70). By collaborating with other positive co-factors such as Hsp40 and the Hsp70-Hsp90 organizing protein (Hop), or competing with negative co-factors such as Bcl2-associated athanogen 1 (Bag1), Hip facilitates may facilitate the chaperone function of Hsc/Hsp70 in protein folding and repair, and in controlling the activity of regulatory proteins such as steroid receptors and regulators of proliferation or apoptosis. Although the nomenclature of ST13 implies a role in the suppression of tumorigenicity (ST), to date available experimental data are not sufficient to support its role in cancer development, except for the possible down-regulation of ST13 in gastric and colorectal cancers. Further investigation of this gene at the physiological level would benefit our understanding of diseases such as endocrinological disorders, cancer, and neurodegeneration commonly associated with protein misfolding. | SHI Zheng-zheng, ZHANG Jia-wei, ZHENG Shu (The Second Affiliated Hospital, Cancer Institute, School of Medicine, Zhejiang University, Hangzhou 310009, China) | 2007 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2007，8，3： | 4 |
| 18 | Induced pluripotent stem cell-related genes influence biological behavior and 5-fluorouracil sensitivity of colorectal cancer cells显示文摘Objective:We aimed to perform a preliminary study of the association between induced pluripotent stem cell(iPS)-related genes and biological behavior of human colorectal cancer(CRC) cells,and the potential for developing anti-cancer drugs targeting these genes.Methods:We used real-time reverse transcriptase polymerase chain reaction(RT-PCR) to evaluate the transcript levels of iPS-related genes NANOG,OCT4,SOX2,C-MYC and KLF4 in CRC cell lines and cancer stem cells(CSCs)-enriched tumor spheres.NANOG was knockdowned in CRC cell line SW620 by lentiviral transduction.3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assays,plate colony formation,and a mouse xenograft model were used to evaluate alterations in biological behavior in NANOG-knockdown SW620 cells.Also,mock-knockdown and NANOG-knockdown cells were treated with 5-fluorouracil(5-FU) and survival rate was measured by MTT assay to evaluate drug sensitivity.Results:A significant difference in the transcript levels of iPS-related genes between tumor spheres and their parental bulky cells was observed.NANOG knockdown suppressed proliferation,colony formation,and in vivo tumorigenicity but increased the sensitivity to 5-FU of SW620 cells.5-FU treatment greatly inhibited the expression of the major stemness-associated genes NANOG,OCT4,and SOX2.Conclusions:These results collectively suggest an overlap between iPS-related genes and CSCs in CRC.Quenching a certain gene NANOG may truncate the aggressiveness of CRC cells. | Zhong SHI1,Rui BAI1,Zhi-xuan FU1,Yong-liang ZHU2,Rong-fu WANG3,Shu ZHENG1(1Cancer Institute(Key Laboratory of Cancer Prevention and Intervention,China National Ministry of Education,Key Laboratory of Molecular Biology in Medical Sciences,Zhejiang Province,China),the Second Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310009,China)(2Department of Gastroenterology,the Second Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310009,China)(3Center for Cell and Gene Therapy,Department of Pathology and Immunology,Baylor College of Medicine,Houston,Texas 77030,USA) | 2012 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2012，13，1： | 4 |
| 19 | Deep lingual arterial chemoembolization of tongue carcinoma with microcapsuled anticancer drug显示文摘Objective: Microcapsule chemoembolism is a promising treatment of tumors. We describe a deep lingual arterial embolization of tongue carcinoma with microcapsuled carboplatinum. Methods: Lingual artery cast specimens from cadavers were microscopically examined, and 78 patients with tongue cancer were recruited and treated with the deep lingual arterial embolization therapy. Results: Microcapsule embolism occurred approximately at the fifth or sixth level of the deep lingual artery branches. The five-year survival rate was 88.5% (69 out of 78), and the ten-year survival rate 52.6% (41 out of 78). Conclusion: The deep lingual arterial embolization of tongue carcinoma with microcapsuled carboplatinum is an effective therapy to treat carcinoma in mid-margin or mid-body of the tongue. | HE Hong1, HUANG Jian-qi1, PING Fei-yun1, CHEN Guan-fu1, ZHANG Su-zhan2 (1Department of Stomatology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China) (2Cancer Institute, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China) | 2007 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2007，8，10： | 3 |
| 20 | Effects of glycine on plasma and liver tissue changes of TNF-α,ET-1 and nitric oxide contents in rats with obstructive jaundice显示文摘AIM: To evaluate the effect of glycine on plasma and liver tissue changes of tumor necrosis factor-α (TNF-α),endothelin-1 (ET-1) and nitric oxide (NO) contents in rats with obstructive jaundice.METHODS: Ninety healthy Wistar rats of both sexes weighing 275±25 g were randomly divided into shamoperated, bile duct-ligated, and bile duct-ligated plus glycine-treated groups, the latter was performed with 5 %glycine solution substituting for tap water drunk ad libium for 5 days before and 6 days after operation. Blood and liver tissue were sampled at the time of sacrifice on the 8th day post operation. Plasma total bilirubin, endotoxin, levels,as well as TNF-α, ET-1 and NO contents in liver tissue were determined.RESULTS: Plasma endotoxin and total bilirubin levels were significantly higher in both bile duct-ligated and bile ductligated plus glycine-treated rats than in sham-operated animals (P=0.000613, 0.00921 and 0.00737, 0.00841respectively), whereas they did not display any statistically significant difference between the former groups (P=0.417and 0.374 respectively). Likewise, TNF-α, ET-1 and NO contents in both plasma and liver tissue were significantly increased in both bile duct-ligated and bile duct-ligated plus glycine-treated rats compared with sham-operated animals (P=-0.00813, 0.00793, 0.00671, 0.00804, 0.00872, and 0.00947 in plasma and 0.00531, 0.00785, 0.00912, 0.00981and 0.00635 in liver tissue respectively). However, these inflammatory mediators in both plasma and liver tissue were significantly reduced in bile duct-ligated rats fed on 5 %glycine solution compared with that without (P=0.00953,0.00891, 0.0795, 0.00867, 0.0697 and 0.00907 in plasma and liver tissue respectively).CONCLUSION: Reduction of TNF-α, ET-1 and NO contents in plasma and liver tissue of rats fed on glycine may be helpful to alleviate pathological lesions in obstructive jaundice. | He-Qing Fang Ying-Bin Liu Hai-Jun Li Shu-You Peng Yu-Lian Wu Bin Xu Jian-Wei Wang Jiang-Tao Li Xin-Bao Wang, Department of General Surgery, Second Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310009, Zhejiang Province, China | 2003 | World Journal of Gastroenterology2003，9，10： | 3 |
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