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| 1 | 小反刍兽疫病毒(PPRV)N蛋白的表达与ELISA检测方法的建立显示文摘通过对下载的16株小反刍兽疫病毒(PPRV)N基因片段进行序列分析,合成了PPRV N基因。用NP1和NP2引物扩增。纯化的PPRV N基因在T4DNA连接酶的作用下,和PET32-a载体进行连接反应构建重组质粒pET-a-PPRV-N。经诱导表达和纯化后,得到了大量的PPRV N蛋白。用PPRV N蛋白制备免疫血清,经国际标准血清标化后作为参考阳性血清,同时以PPRV N蛋白为抗原建立间接ELISA(I-ELISA)方法。对山东省的467份羊血清检测,使用S/P(样品值/阳性值)平均值加3倍标准差的方法,计算出其临界点为0.28。应用间接ELISA和针对H蛋白的单抗为基础的C-ELISA检测来自于疫区的69份血清,结果两者的符合率为79.7%。 | 张喜悦 王志亮 刘春菊 李林 包静月 何昭阳 Mandy C Carrie B Anderson J | 2008 | 中国兽医学报2008，28，2： | 8 |
| 2 | Association between calcium sensing receptor gene polymorphisms and chronic pancreatitis in a US population:Role of serine protease inhibitor Kazal 1type and alcohol显示文摘AIM: To test the hypothesis that calcium sensing receptor (CASR) polymorphisms are associated with chronic pancreatitis (CP), and to determine whether serine protease inhibitor Kazal 1type (SPINK1) N34S oralcohol are necessary co-factors in its etiology. METHODS: Initially, 115 subjects with pancreatitis and 66 controls were evaluated, of whom 57 patients and 21 controls were predetermined to carry the high-risk SPINK1 N34S polymorphism. We sequenced CASR gene exons 2, 3, 4, 5 and 7, areas containing the majority of reported polymorphisms and novel mutations. Based on the initial results, we added 223 patients and 239 controls to analyze three common nonsynonymous single nucleotide polymorphisms (SNPs) in exon 7 (A986S, R990G, and Q1011E). RESULTS: The CASR exon 7 R990G polymorphism was signifi cantly associated with CP (OR, 2.01; 95% CI, 1.12-3.59; P = 0.015). The association between CASR R990G and CP was stronger in subjects who reported moderate or heavy alcohol consumption (OR, 3.12; 95% CI, 1.14-9.13; P = 0.018). There was no association between the various CASR genotypes and SPINK1 N34S in pancreatitis. None of the novel CASR polymorphisms reported from Germany and India was detected. CONCLUSION: Our United States-based study confirmed an association of CASR and CP and for the first time demonstrated that CASR R990G is a signifi cant risk factor for CP. We also conclude that the risk of CP with CASR R990G is increased in subjects with moderate to heavy alcohol consumption. | Venkata Muddana Janette Lamb Julia B Greer Beth Elinoff Robert H Hawes Peter B Cotton Michelle A Anderson Randall E Brand Adam Slivka David C Whitcomb | 2008 | World Journal of Gastroenterology2008，14，28： | 7 |
| 3 | Structral Characteristics of Steam-Flaked Sorghum显示文摘 | C M McDonough B J Anderson L W Rooney | 1997 | Cereal Chem1997，74，5： | 1 |
| 4 | Differentiation of human adipose tissue stem cells using extracts of rat cardiomyocytes 显示文摘 | Gaustad K G Boquest A C Anderson B E | 2004 | Biochem Biophys Res Commun2004，314，2： | 1 |
| 5 | Factorial invariance of LibQUAL + as a measure of library service quality over time 显示文摘 | Lane F C Anderson B Ponce H F | 2012 | Library & Information Science Research2012，34，1： | 1 |
| 6 | Harvesting a crop of gold in plants显示文摘 | Anderson C W N Brooks R R Stewart R B | 1998 | Nature1998，395，6702： | 1 |
| 7 | The role of IL-113 and TNF-ct signaling in the genesis of cancer treatment related symptoms(CTRS):a study using cytokine receptor--deficient mice显示文摘 | SMITH L B LEO M C ANDERSON C | 2014 | Brain Behavior and Immunity2014，，38： | 1 |
| 8 | Sonic hedgehog acts cell-autonomously on muscle precursor cells to generate limb muscle diversity显示文摘 | Anderson C Williams VC Moyon B | 2012 | Genes Dev2012，26，18： | 1 |
| 9 | Sex and diploidy in Armillaria mellea显示文摘 | Ullrich R C Anderson J B | 1978 | Experimental Mycology1978，2，： | 1 |
| 10 | Relationships between European and North American biological species of Armillaria mellea 显示文摘 | Anderson J B Korhonen K Ullrich R C | 1980 | Experimental Mycology1980，4，： | 1 |
| 11 | Attenuation of Seismic Energy in the Upper Mantle显示文摘 | Menahen A B Archambeau C B | 1965 | Journal of Geophysical Research1965，70，： | 1 |
| 12 | Cryptosporidiosis in bovine and human health显示文摘 | ANDERSON B C | 1998 | J Dairy Sci1998，81，： | 1 |
| 13 | Development of a species-specific marker using RAPD analysis to distinguish between sheep and goats显示文摘 | Cargill S L Anderson C B Medrano J F | 1995 | Animal Biotch1995，6，2： | 1 |
| 14 | Reanalysis and results after 12 years off following-up in a randomized clinical trial comparing total mastectomy with lumpectomy with or without irradiation in the treatment of breast cancer显示文摘 | Fisher B Anderson S Redmond C K | 1995 | N En J Med1995，333，22： | 1 |
| 15 | Oil/water separation with surfacemodified membranes 显示文摘 | ANDERSON G K SAW C B | 1987 | Environ Tech Lett1987，8，： | 1 |
| 16 | A comparison of sedimentary and diatom-inferred phosphorus profiles:implications for defining pre-disturbance nutrient conditions显示文摘 | Anderson N J Rippey B Gibson C E | 1993 | Hydrobiologia1993，253，： | 1 |
| 17 | Bacterial elicitation and evasion of plant innate immunity 显示文摘 | Abramovitch R B Anderson J C Martin G B | 2006 | Nat Rev Mol Cell Biol2006，7，8： | 1 |
| 18 | The ballistic performance of confined Al2O3 ceramic tiles 显示文摘 | Anderson C E Morris B L | 1992 | Int J Impact Eng1992，12，2： | 1 |
| 19 | Com- bining value and price to make purchase decisions in business markets显示文摘 | Anderson J C Thomson J B L Wynstra F | 2000 | Intermational Journal of Research in Marketing2000，17，4： | 1 |
| 20 | The American customer satisfaction index: nature, purpose, and findings 显示文摘 | Fornell C Johnson M D Anderson E W Cha J S Bryant B E | 1996 | Journal of Marketing1996，60，4： | 1 |
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