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| 1 | 黑曲霉葡萄糖氧化酶基因的克隆及其在酵母中的高效表达显示文摘将黑曲霉葡萄糖氧化酶 (GOD)基因重组进大肠杆菌 酵母穿梭质粒pPIC9,转化甲基营养酵母Pichiapasto risGS115 ,构建出GOD的高产酵母工程菌株。在酵母α Factor及AOX1基因启动子和终止信号的调控下 ,黑曲霉GOD在甲基酵母中大量表达并分泌至胞外 ,经甲醇诱导 3~ 4d ,发酵液中的GOD活力可达 30~ 40u mL。SDS PAGE证实GOD在培养物上清中的含量显著高于其它杂蛋白 ,约占胞外蛋白总量的 6 0 %~ 70 % ,经QSepharoseTM FastFlow离子交换柱一步纯化即达电泳纯。重组酵母GOD比活达 42 6 6 3u mg蛋白 ,是商品黑曲霉GOD的 1 6倍。动力学性质分析表明 ,重组酵母GOD的Km 和kcat分别为 38 2 5mmol L和 34 92 6 6s- 1 ,与商品黑曲霉GOD相比 ,具有更高的催化效率。重组酵母GOD的高活力特性可有效提高葡萄糖传感器的线性检测范围。 | 周亚凤 张先恩 刘虹 张成刚 Anthony E G Cass | 2001 | 生物工程学报2001，17，4： | 25 |
| 2 | Constitutive STAT3 phosphorylation and IL-6/IL-10 co-expression are associated with impaired T-cell function in tuberculosis patients显示文摘T-cells critically contribute to protection against Mycobacterium tuberculosis infection,and impaired T-cell responses can lead to disease progression.Pro-inflammatory and immunosuppressive cytokines affect T-cells,and fine-tuned regulation of cytokine signaling via the Jak/STAT signaling pathways is crucial for appropriate T-cell function.Constitutive STAT3 phosphorylation as a consequence of aberrant cytokine signaling has been described to occur in pathognomonic T-cell responses in inflammatory and autoimmune diseases.We characterized blood samples from tuberculosis patients(n=28)and healthy contacts(n=28)from Ghana for M.tuberculosis-specific T-cell responses,constitutive cytokine production,and SOCS3 and pSTAT3 expression.Lentiviral modulation of primary CD4+T-cells was performed to determine the effects of SOCS3 on T-cell functions.T-cells from tuberculosis patients expressed higher levels of IL-10 and IL-6 and lower levels of T helper type(TH)17 cytokines after M.tuberculosis-specific stimulation compared to healthy contacts.In addition,tuberculosis patients had higher IL-10 and IL-6 levels in the supernatants of non-stimulated immune cells and plasma samples compared to healthy contacts.Notably,aberrant cytokine expression was accompanied by high constitutive pSTAT3 levels and SOCS3 expression in T-cells.Multivariate analysis identified an IL-6/IL-10 co-expression-based principal component in tuberculosis patients that correlated with high pSTAT3 levels.SOCS3 contributed to a regulatory component,and tuberculosis patients with high SOCS3 expression showed decreased TH1 cytokine expression and impaired IL-2-induced STAT5 phosphorylation.SOCS3 over-expression in primary CD4+T-cells confirmed the SOCS3 inhibitory function on IL-2-induced STAT5 phosphorylation.We conclude that constitutive pSTAT3 and high SOCS3 expression are influential factors that indicate impaired T-cell functions in tuberculosis patients. | Kirstin Harling Ernest Adankwah Alptekin Güler Anthony Afum-Adjei Awuah Louis Adu-Amoah Ertan Mayatepek Ellis Owusu-Dabo Norman Nausch Marc Jacobsen | 2019 | Cellular & Molecular Immunology2019，16，3： | 15 |
| 3 | 黄芪总黄酮对扑热息痛所致肝损伤的防护机理探讨显示文摘目的 :研究黄芪总黄酮对扑热息痛所致肝损伤的防护机理。方法 :用高压液相测定扑热息痛及代谢产物和苯巴比妥诱导睡眠模型研究黄芪总黄酮 (TFA)对扑热息痛的代谢及TFA抗扑热息痛所致损伤的机理。结果 :TFA可明显降低尿中硫醚胺酸的浓度 ,其变化与TFA浓度成反比 ;但对其它代谢产物的影响无明显统计学差异。苯巴比妥诱导小鼠睡眠实验结果显示 ,TFA (10 0mg·kg-1)本身对苯巴比妥代谢无明显影响 (P >0 .0 5 ) ,扑热息痛 (40 0mg·kg-1)可显著延长小鼠的睡眠时间 (P <0 .0 0 1) ;但TFA (10 0mg·kg-1)明显缩短扑热息痛所延长的苯巴比妥诱导的睡眠时间 (P <0 .0 0 5 )。结论 | 汪德清 Critchley Julian AJH 丁保国 马艳青 赵海潞 Thomas G Neil 田亚平 Tomlinson B rian Critchley Lester AH James Anthony Edward | 2001 | 中国中药杂志2001，26，9： | 13 |
| 4 | Fate and Transport of Antibiotic Residues and Antibiotic Resistance Genes following Land Application of Manure Waste显示文摘 | Chee-Sanford Joanne C Mackie Roderick I Koike Satoshi Krapac Ivan G Lin Yu-Feng Yannarell Anthony C Maxwell Scott Aminov Rustam I | 2009 | Journal of Environmental Quality2009，，3： | 3 |
| 5 | Fondaparinux预防老年急性内科患者发生静脉血栓形成的效果与安全性:随机安慰剂对照研究显示文摘目的:观察 Fondaparinux 对具有中高度静脉血栓发生危险的老年急性内科住院患者的抗凝效果与安全性。设计:双盲随机安慰剂对照研究。背景:8个国家的35个中心。参与者:849例≥60岁内科患者,住院原因分别为充血性心力衰竭、慢性肺病合并急性呼吸系统疾患、急性炎症性或感染性疾病,预期至少住院4天以上。干预:2.5 mg Fondaparinux 或安慰剂,每天1次皮下注射,持续6~14天。观察指标:主要指标为静脉血栓形成(治疗后15天内采用双侧静脉造影检查)及有症状的静脉血栓;次要指标为死亡与出血。患者随访时间为1个月。结果:Fondaparinux 治疗组425例患者和安慰剂组414例患者接受了安全性分析(10例未治疗)。644例患者(75.9%)可接受主要指标分析。静脉血栓检出率在 Fondaparinux 治疗组为5.6%(18/321),安慰剂组为10.5%(34/323),相对危险减少46.7%(95% CI 7.7%~69.3%)。安慰剂组5例患者发生有症状的静脉血栓,Fondaparinux治疗组无患者发生有症状的静脉血栓(P=0.029)。两组均有1例(0.2%)患者发生严重出血。随访结束时,安慰剂组、Fondaparinux 治疗组分别死亡25(6.0%)、14(3.3%)例患者。结论:Fondaparinux 可有效预防急性内科老年患者无症状性及有症状的静脉血栓。严重出血几率两组相似。 | Alexander T Cohen Bruce L Davidson Alexander S Gallus Michael R Lassen Martin H Prins Witold Tomkowski Alexander G G Turpie Jan F M Egberts Anthonie W A Lensing 石汉平(译) 王深明(校) | 2006 | 英国医学杂志中文版2006，9，5： | 3 |
| 6 | Human acute myelogenous leukemia stem cells are rare and heterogeneous when assayed in NOD/SCID/IL2R[gamma]c-deficient mice显示文摘 | Sarry Jean-Emmanuel Murphy Kathleen Perry Robin Sanchez Patricia V Secreto Anthony Keefer Cathy Swider Cezary R Strzelecki Anne-Claire Cavelier Cindy Récher Christian Mansat-De Mas Véronique Delabesse Eric Danet-Desnoyers G Carroll Martin | 2011 | Journal of Clinical Investigation2011，，1： | 2 |
| 7 | 显示文摘 | Wilk G D Wallace R M Anthony J M | 2001 | Journal of Applied Physics2001，89，10： | 2 |
| 8 | Management of esophageal caustic injury显示文摘Ingestion of caustic substances and its long-term effect on the gastrointestinal system maintain its place as an important public health issue in spite of the multiple efforts to educate the public and contain its growing number. This is due to the ready availability of caustic agents and the loose regulatory control on its production. Substances with extremes of pH are very corrosive and can create severe injury in the upper gastrointestinal tract. The severity of injury depends on several aspects: Concentration of the substance, amount ingested, length of time of tissue contact, and p H of the agent. Solid materials easily adhere to the mouth and pharynx, causing greatest damage to these regions while liquids pass through the mouth and pharynx more quickly consequently producing its maximum damage in the esophagus and stomach. Esophagogastroduodenoscopy is therefore a highly recommended diagnostic tool in the evaluation of caustic injury. It is considered the cornerstone not only in the diagnosis but also in the prognostication and guide to management of caustic ingestions. The degree of esophageal injury at endoscopy is a predictor of systemic complication and death with a 9-fold increase in morbidity and mortality for every increased injury grade. Because of this high rate of complication, prompt evaluation cannot be overemphasized in order to halt development and prevent progression of complications. | Mark Anthony A De Lusong Aeden Bernice G Timbol Danny Joseph S Tuazon | 2017 | World Journal of Gastrointestinal Pharmacology and Therapeutics2017，8，2： | 2 |
| 9 | Ultra-rapid freezing of water treatment residuals显示文摘 | Philip J Parke Anthony G Collins | 1999 | Wat Res1999，33，10： | 1 |
| 10 | Optieal fiber sensor technology显示文摘 | Thomas G GlaUorenzi Joseph A Buearo Anthony Dandridge | 1982 | IEEE J Quantum Eleetronies1982，18，4： | 1 |
| 11 | Fatal case of toxic shock-like syndrome due to group C Streptococcus 'associated with superantigen exotoxin 显示文摘 | Tony M K Anthony B TravisM G | 2004 | J Clin Microbiol2004，42，： | 1 |
| 12 | 查看详情显示文摘 | Chun J W Anthony R G | | 0，，： | 1 |
| 13 | Protein engineering of α - amylase for low pH performance 显示文摘 | Andrew S Richard B Anthony G D | 1999 | Current Opinion in Biotechnology1999，10，4： | 1 |
| 14 | Ocean acidification causes bleaching and productivity loss in coral reef builders显示文摘 | Anthony K R N Kline D I INaz Pulido G | 2008 | Proceedings of the National Academy of Sciences of the United States of America2008，105，17： | 1 |
| 15 | Broad diversity of neutralizing antibodies isolated from memory B cells in HIV-infected individuals显示文摘 | Scheid J F Mouquet H Feldhahn N Seaman M S Velinzon K Pietzsch J Ott R G Anthony R M Zebroski H Hurley A Phogat A Chakrabarti B Li Y Connors M Pereyra F Walker B D Wardemann H Ho D Wyatt R T Mascola J R Ravetch J V Nussenzweig M C | 2009 | Nature2009，458，7238： | 1 |
| 16 | Agricultural nitrogen balance and water quality in the UK显示文摘 | LORD E I ANTHONY S G GOODLASS G | 2002 | Soil Use and Management2002，18，4： | 1 |
| 17 | Antiarthritie effect of lupeol acetate显示文摘 | Kweifio-OKai G Carroll Anthony R Carroll | 1993 | Phytotherapy Research1993，7，2： | 1 |
| 18 | Electrospinning and dtabilization of gfully hydrolrzed poly(viny alcohol)fibers显示文摘 | YAO LI THOMAS W H ANTHONY G E | 2003 | Chem Mater2003，15，： | 1 |
| 19 | Photocatalytic degradation for environmental applications:a review显示文摘 | Dhananjay S Bhatkhande Vishwas G Pangarkar Anthony ACM Beenackers | 2001 | Journal of Chemical Technology and Biotechnology2001，77，2： | 1 |
| 20 | Repartition effect of aromatic polyaniline coatings on the separation of bioactive peptides in capillary electrophoresis显示文摘 | ALESSANDRA B SERGEY A P ANTHONY P F T PIER G R | 2002 | Electrophoresis2002，23，2： | 1 |
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