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| 1 | Isolation and diagnosis of Helicobacter pylori by a new method: Microcapillary culture显示文摘AIM:To investigate the performance of the microcapillary culture method(MCM) in Helicobacter pylori(H.pylori) isolation and diagnosis.METHODS:Microcapillary culture(MC),classical culture(CC),rapid urease(CLO) test,and histopathologic examination(HE) were performed with biopsy samples.Homogenized biopsy samples were loaded into capillary tubes and incubated for 48 h at 37 ℃ without providing a microaerophilic environment.Additionally,three or four loops of the homogenized sample were inoculated in a ready-to-use selective medium(Becton Dickinson,Helicobacter Agar,Modified) specific for the isolation of H.pylori and incubated at 37 ℃ in a microaerophilic atmosphere provided by Campy Gen(Becton Dickinson,Gas Pack).Bacteria reproducing in microcapillary tubes were evaluated in an inverted microscope and also were evaluated after performing a CC with the content.Results obtained by CC,CLO test,and HE were compared with those of MC.The diagnostic performances of the methods used in this study were evaluated for specificity,sensitivity,positive predictive value(PPV),negative predictive value(NPV),and CI.RESULTS:H.pylori was found positive by CLO test +HE and/or CC culture in 26 patient antrum and corpus biopsy samples.In 25(25/26) patient biopsy samples,H.pylori was isolated by MCM,whereas in only 14(14/26) patient biopsy samples,H.pylori was isolatedby CC.CLO test and HE were found positive in 17(17/26) patient biopsy samples.Comparing the results of the isolation of H.pylori by MCM,CC,CLO test,and HE,the sensitivity of the MCM was found as 96%,the specificity as 80%,the PPV as 83%,the NPV as 95%,and the 95%CI as 0.76(χ2 =31.51,P < 0.01) whereas the sensitivity of the CC was found as 54%(χ2 =19.15,P < 0.01),and the sensitivity of the CLO test and HE were found as 65%(χ2 =25.26,P < 0.01).CONCLUSION:This new microcapillary cultivation method for H.pylori has high diagnostic sensitivity compared with CC,HE,and CLO tests. | Adil M Allahverdiyev Melahat Bagirova Reyhan Caliskan Hrisi Bahar Tokman Hayat Aliyeva Gokce Unal Olga Nehir Oztel Emrah Sefik Abamor Hilal Toptas Pelin Yuksel Fatma Kalayci Mustafa Aslan Yusuf Erzin Kadir Bal Bekir S Kocazeybek | 2015 | World Journal of Gastroenterology2015，21，9： | 2 |
| 2 | Inferring the origin and dietary history of beef from C,N and S stable isotope ratio analysis显示文摘 | Schmidt O Quilter J M Bahar B | 2005 | Food Chemistry2005，91，： | 1 |
| 3 | Channeled coolant plate: A new method to enhance freshwater production from an air gap membrane distillation (AGMD) desalination unit显示文摘 | Bahar R Hawlader M N A AriffT F | 2015 | Desalination2015，359，： | 1 |
| 4 | Neurological toxicity of the subarachnoid infusion of bupivacaine,lignocaine or 2-chloroprocaine in the rat显示文摘 | Li DF Bahar M Cole G | 1985 | Br J Anaesth1985，57，4： | 1 |
| 5 | Risk factors and pregnancy outcome in different types of placenta previa 显示文摘 | Bahar A Abusham A Eskandar M | 2009 | J Obstet Gynaecol Can2009，，2： | 1 |
| 6 | Performance of com-pacted cement-stabilised soil显示文摘 | Bahar R Benazzoug M Kenai S | 2004 | Cement ConcreteComposit2004，，26： | 1 |
| 7 | Risk factorsand pregnancy outcome in different types of placenta previa显示文摘 | BAHAR A ABUSHAM A ESKANDAR M | 2009 | J Obstet Gynaecol Can2009，31，2： | 1 |
| 8 | A Review on Mobile Cloud Computing显示文摘 | Shamim S M Sarker A Bahar A N | 2015 | International Journal of Computer Applications2015，113，16： | 1 |
| 9 | Neurological Toxicity of the Subarachnoid Infusion of Bupivacaine, Lignocaine or 2-chloroprocaine in the Rat显示文摘 | Li D F Bahar M Cole G | 1985 | Br J Anaesth1985，57，4： | 1 |
| 10 | Cyclocryotherapy versus transscleral diode laser cyclophotocoagulation for uncontrolled intraocular pressure显示文摘 | Goldenberg-Cohen N Bahar I Ostashinski M | 2005 | Ophthalmic Surg Lasers Imaging2005，36，4： | 1 |
| 11 | Inferring the origin and dietary history of beef from C, N and S stable isotope ratio analysis显示文摘 | Schmidt O Quilter J M Bahar B | 2005 | Food Chemistry2005，91，： | 1 |
| 12 | Relationship between river water quality and land use in a small river basin running through the urbanizing area of Central Japan显示文摘 | Bahar M M Ohmori H Yamamuro M | 2008 | Limnology2008，9，： | 1 |
| 13 | Severe pre-eclampsia and eclampsia in Abha,the south west region of Saudi Arabia显示文摘 | Sobande A A Eskandar M Bahar A | 2007 | J Obstet Gynaecol2007，27，2： | 1 |
| 14 | Inferring the origin and dietary history of beef from C,N and S stable isotope ratio analysis显示文摘 | Schmidt O Quilter J M Bahar B | 2005 | Food Chemistry2005，91，3： | 1 |
| 15 | Spinal anaesthesia with midazo- lam in the rat显示文摘 | Bahar M Cohen ML Grinshpon Y | 1997 | Can J Anaesth1997，44，2： | 1 |
| 16 | Comparison of WBC,ESR,CRP and PCT serum levels in septic and non-septic burn cases显示文摘 | Barati M Alinejad F Bahar MA | 2008 | Burns2008，34，6： | 1 |
| 17 | Comparison of WBC, ESR, CRP and PCT serum levels in septic and non-septic burn cases显示文摘 | Barati M Alinejad F Bahar MA | 2008 | Burns2008，34，6： | 1 |
| 18 | Mycoplasma pneumonia seroposivity in Iranian patients with relapsing-remitting muhipl seerosis: a random- ized case-control study显示文摘 | Bahar M Ashtari F Aghaei M | 2012 | J Pak Med Assoc2012，62，3： | 1 |
| 19 | What to expect after tricuspid valve replacement long-term results 显示文摘 | Iscan Z H Vural K M Bahar I | 2007 | Eur J Cardio Thorac Surg2007，32，2： | 1 |
| 20 | Inferring the origin and dietary history of beef from C, N and S stable isotope ratio analysis显示文摘 | SCHMIDT O QUILTER J M BAHAR B | 2005 | Food Chem2005，91，： | 1 |
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