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146篇 您的检索式：作者名="BEARD H"

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	题名	作者	年代	出处	被引量
1	Osteopontin increases hepatocellular carcinoma cell growth in a CD44 dependant manner显示文摘AIM:To investigate the role of osteopontin(OPN) and its splice variants in the proliferation of hepatocellular carcinoma(HCC).METHODS:The expression of OPN variants in HCC cell lines as well as HCC tissue samples and nontumour tissue was studied using polymerase chain reaction.OPN variant cDNAs were cloned into a mammalian expression vector allowing both transient expression and the production of stable OPN expressing cell lines.OPN expression was studied in these cells using Western blotting,immunofluoresnce and enzyme linked immunosorbent assay.A CD44 blocking antibody and siRNA targeting of CD44 were used to examine the role of this receptor in the OPN stimulated cell growth observed in culture.Huh-7 cells stably expressing either OPN-A,-B or-C were injected subcutaneously into the flanks of nude mice to observe in vivo tumour growth.Expression of OPN mRNA and protein in these tumours was examined using reverse transcriptionpolymerase chain reaction and immunohistochemistry.RESULTS:OPN is expressed in HCC in 3 forms,the full length OPN-A and 2 splice variants OPN-B and-C.OPN variant expression was noted in HCC tissue as well as cognate surrounding cirrhotic liver tissue.Expression of these OPN variants in the HCC derived cell line Huh-7 resulted in secretion of OPN into the culture medium.Transfer of OPN conditioned media to na ve Huh-7 and HepG2 cells resulted in significant cell growth suggesting that all OPN variants can modulate cell proliferation in a paracrine manner.Furthermore the OPN mediated increase in cellular proliferation was dependent on CD44 as only CD44 positive cell lines responded to OPN conditioned media while siRNA knockdown of CD44 blocked the proliferative effect.OPN expression also increased the proliferation of Huh-7 cells in a subcutaneous nude mouse tumour model,with Huh-7 cells expressing OPN-A showing the greatest proliferative effect.CONCLUSION:This study demonstrates that OPN plays a significant role in the proliferation of HCC through interaction with the cell surface receptor CD44.Modulation of this interaction could represent a novel strategy for the control of HCC.	Renee J Phillips Karla J Helbig Kylie H Van der Hoek Devanshi Seth Michael R Beard	2012	World Journal of Gastroenterology2012，18，26：	12
2	Structure and mechanism of DNA polymerase Beta显示文摘	BEARD W A WILSON S H	2006	Chem Rev2006，106，2：	1
3	Subsidy free pricing of interruptible service contracts 显示文摘	BEARD T R SWEENEY H GROPPER D M	1995	Energy Economics1995，17，1：	1
4	Combined anterior cruciate reconstruction and Oxford unicompartmental knee arthroplasty显示文摘	Pandit H Beard DJ Jenkins C		0，，07：	1
5	The mitochondrial genome of the mosquito Anopheles gambiae:DNA sequence,genome organization,and comparisons with mitochondrial sequences of other insects显示文摘	Beard C B Hamm D M Collins F H	1993	Insect Molecular Biology1993，2，2：	1
6	Iron isotope biosignatures显示文摘	Beard B Johnson C Cox L Sun H N ealson K and Aguilar C		0，，5434：	1
7	Starch Gel Electrophoresis Used for Identification of Turf-type Cynodon Genotypes 显示文摘	Vermeulen P H Beard J B	1991	Crop Science1991，，31：	1
8	Comparative dehydration avoidance and drought resistance among major warm-season turfgrass species and cuhivars显示文摘	Sifers S I Beard J B Hall M H	1990	Texas Agricultural1990，3740，：	1
9	A wind tunnel investigation of the internal circulation and shape of water drops falling at terminal velocity in air显示文摘	Pruppacher H R Beard K V	1970	Q J Roy Meteor Soc1970，96，：	1
10	Structure and mechanism of DNA polymerase beta显示文摘	Beard W A Wilson S H		0，，2：	1
11	Separation of arsenic from antimony and bismuth by solvent extraction显示文摘	Beard H C Lyerly L A	1991	Anal Chem1991，33，12：	1
12	Cementless Oxford unicompartmental knee replacement shows reduced radiolucency at one year显示文摘	Pandit H Jenkins C Beard DJ		0，，02：	1
13	Molecular and epidemiologic trends of caliciviruses associated with outbreak of acute gastroenteritis in the United States ( 2000 -2004 ) 显示文摘	BLANTON L H ADAMS S M BEARD R S	2006	J infect Dis2006，193，3：	1
14	Application of Fe isotopes to tracing the geochemical and biological cycling of Fe显示文摘	Beard B L Johnson C M Skulan J L Nealson K H Cox L Sun H		0，，：	1
15	The mechanisms of iron isotope fractionation produced during dissimilatory Fe(Ⅲ) reduction by Shewanella putrefaciens and Geobacter sulfurreducens显示文摘	Crosby H A Roden E E Johnson C M Beard B L		0，，02：	1
16	Pre-operative clinical and radio-logical assessment of the patellofemoral joint in unicompartmental knee replacement and its influence on outcome显示文摘	Beard DJ Pandit H Murray DW		0，，12：	1
17	A wind tunnelinvestigation of the internal circulation and shape ofwater drops falling at terminal velocity in air显示文摘	PRUPPACHER H R BEARD K V	1970	The Quarterly Journal of the Royal MeteorologicalSociety1970，96，408：	1
18	Bradykinin augments insulin-stimulated glucose transport in rat adipocytes via endothelial nitric oxide synthase-mediated inhibition of Jun NH2-terminal kinase显示文摘	Beard K M Lu H Ho K	2006	Diabetes2006，55，10：	1
19	Vision-based navigation frame mapping and planning for collision avoidance for miniature airvehicles显示文摘	YU H BEARD R BYRNE J	2010	Control Engineering Practice2010，18，7：	1
20	Solar cells from colloidal nanocrystals: Fundamentals, materials, devices, and economics显示文摘	Hillhouse H W Beard M C	2009	Current Opin-ion in Colloid & Interface Science2009，14，4：	1

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