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| 1 | Osteopontin increases hepatocellular carcinoma cell growth in a CD44 dependant manner显示文摘AIM:To investigate the role of osteopontin(OPN) and its splice variants in the proliferation of hepatocellular carcinoma(HCC).METHODS:The expression of OPN variants in HCC cell lines as well as HCC tissue samples and nontumour tissue was studied using polymerase chain reaction.OPN variant cDNAs were cloned into a mammalian expression vector allowing both transient expression and the production of stable OPN expressing cell lines.OPN expression was studied in these cells using Western blotting,immunofluoresnce and enzyme linked immunosorbent assay.A CD44 blocking antibody and siRNA targeting of CD44 were used to examine the role of this receptor in the OPN stimulated cell growth observed in culture.Huh-7 cells stably expressing either OPN-A,-B or-C were injected subcutaneously into the flanks of nude mice to observe in vivo tumour growth.Expression of OPN mRNA and protein in these tumours was examined using reverse transcriptionpolymerase chain reaction and immunohistochemistry.RESULTS:OPN is expressed in HCC in 3 forms,the full length OPN-A and 2 splice variants OPN-B and-C.OPN variant expression was noted in HCC tissue as well as cognate surrounding cirrhotic liver tissue.Expression of these OPN variants in the HCC derived cell line Huh-7 resulted in secretion of OPN into the culture medium.Transfer of OPN conditioned media to na ve Huh-7 and HepG2 cells resulted in significant cell growth suggesting that all OPN variants can modulate cell proliferation in a paracrine manner.Furthermore the OPN mediated increase in cellular proliferation was dependent on CD44 as only CD44 positive cell lines responded to OPN conditioned media while siRNA knockdown of CD44 blocked the proliferative effect.OPN expression also increased the proliferation of Huh-7 cells in a subcutaneous nude mouse tumour model,with Huh-7 cells expressing OPN-A showing the greatest proliferative effect.CONCLUSION:This study demonstrates that OPN plays a significant role in the proliferation of HCC through interaction with the cell surface receptor CD44.Modulation of this interaction could represent a novel strategy for the control of HCC. | Renee J Phillips Karla J Helbig Kylie H Van der Hoek Devanshi Seth Michael R Beard | 2012 | World Journal of Gastroenterology2012，18，26： | 12 |
| 2 | Through-needle all-optical ultrasound imaging in vivo: a preclinical swine study显示文摘High-frequency ultrasound imaging can provide exquisite visualizations of tissue to guide minimally invasive procedures.Here,we demonstrate that an all-optical ultrasound transducer,through which light guided by optical fibers is used to generate and receive ultrasound,is suitable for real-time invasive medical imaging in vivo.Broad-bandwidth ultrasound generation was achieved through the photoacoustic excitation of a multiwalled carbon nanotube-polydimethylsiloxane composite coating on the distal end of a 300-μm multi-mode optical fiber by a pulsed laser.The interrogation of a high-finesse Fabry–Pérot cavity on a single-mode optical fiber by a wavelength-tunable continuous-wave laser was applied for ultrasound reception.This transducer was integrated within a custom inner transseptal needle(diameter 1.08 mm;length 78 cm)that included a metallic septum to acoustically isolate the two optical fibers.The use of this needle within the beating heart of a pig provided unprecedented realtime views(50 Hz scan rate)of cardiac tissue(depth:2.5 cm;axial resolution:64μm)and revealed the critical anatomical structures required to safely perform a transseptal crossing:the right and left atrial walls,the right atrial appendage,and the limbus fossae ovalis.This new paradigm will allow ultrasound imaging to be integrated into a broad range of minimally invasive devices in different clinical contexts. | Malcolm C Finlay Charles A Mosse Richard J Colchester Sacha Noimark Edward Z Zhang Sebastien Ourselin Paul C Beard Richard J Schilling Ivan P Parkin Ioannis Papakonstantinou Adrien E Desjardins | 2017 | Light(Science & Applications)2017，6，1： | 6 |
| 3 | Current role of palliative interventions in advanced pancreatic cancer显示文摘Pancreatic adenocarcinoma is the third leading cause of cancer death in the United States. Unfortunately, at diagnosis, most patients are not candidates for curative resection. Surgical palliation, a procedure performed with the intention of relieving symptoms or improving quality of life, comes to the forefront of management. This article reviews the palliative management of unresectable pancreatic cancer, including obstructive jaundice, duodenal obstruction and pain control with celiac plexus block. Although surgical bypasses for both biliary and duodenal obstructions usually achieve good technical success, they result in considerable perioperative morbidity and mortality, even when performed laparoscopically. The effectiveness of selfexpanding metal stents for biliary drainage is excellent with low morbidity. Surgical gastrojejunostomy for duodenal obstruction appears to be best for patients with a life expectancy of greater than 2 mo while endoscopic stenting has been shown to be feasible with good symptom relief in those with a shorter life expectancy. Regardless of the palliative procedure performed, all physicians involved must be adequately trained in end of life management to ensure the best possible care for patients. | Chelsey C Ciambella Rachel E Beard Thomas J Miner | 2018 | World Journal of Gastrointestinal Surgery2018，10，7： | 4 |
| 4 | 年龄相关性线粒体功能障碍加速动脉粥样硬化形成显示文摘老化是动脉粥样硬化的最强危险因素之一。然而,老化是否独立于慢性高血脂症而增加动脉粥样硬化的风险尚不清楚。该研究旨在确定高血脂症诱导前的血管老化是否会加速动脉粥样硬化的形成。研究者分析了幼年和老年正常血脂野生型(wild type,WT)、无疾病小鼠的主动脉,发现老化导致白细胞介素(interleukin,IL)6水平升高和线粒体功能障碍,并与线粒体自噬增加和其相关蛋白Parkin增加相关。在主动脉组织培养中,研究者发现随着老化的出现,线粒体功能障碍与IL-6存在正反馈关系。研究者通过诱导低密度脂蛋白受体的肝脏特异性降解,再结合10周的西方食物喂养,在老年和幼年小鼠中诱发急性高血脂症,发现老年WT小鼠的动脉粥样硬化增强。高血脂症进一步降低了老年小鼠的线粒体功能,增加了主动脉中Parkin的水平,但幼鼠则没有。 | 刘青(译) 叶鹏(摘、审校) Tyrrell DJ Blin M Song J Wood S Zhang M Beard DA Goldstein D | 2020 | 中华高血压杂志2020，28，2： | 2 |
| 5 | Synchronized multiple spacecraft rotations显示文摘 | Lawton J R Beard R W | 2002 | Automatic2002，38，8： | 1 |
| 6 | The time of death after trauma显示文摘 | Wyatt J Beard D Gray A | 1995 | BMJ1995，310，： | 1 |
| 7 | Comparative turfgrass evapotranspiration rates and associated plant morphological characteristics显示文摘 | Kim K S Beard J B | 1988 | Crop Sci1988，28，： | 1 |
| 8 | Mammalian X chromosome inactivation 显示文摘 | Jaenisch R Beard C Lee J | 1998 | Novartis Found Symp1998，214，： | 1 |
| 9 | Nitric oxide regula- tion of gonadotrophin seeretion in prepubertal heifers显示文摘 | Honaramooz A Cook S J Beard A P et ol | 1999 | J Neu- roendoerinol1999，11，9： | 1 |
| 10 | Pneumocystis colonisation is common among hospitalised HIV infected patients with nonpneumocystis pneumonia显示文摘 | Davis J L Welsh D A Beard C B | 2008 | Thorax2008，63，4： | 1 |
| 11 | A sustainable solution for dietary iron deficiency through plant biotechnology and breeding to increase seed ferritin control显示文摘 | Theil E C Burton J W Beard J L | 1997 | European Journal of Clinical Nutrition1997，51，4： | 1 |
| 12 | Non-destructive testing of rock bolts using guided ultrasonic waves显示文摘 | Beard M D Lowe M J S | 2003 | Intl Journal of Rock Mechanics & Mining Science2003，40，： | 1 |
| 13 | Randomized, multicentre, doudle-blind, placebo-controlled trial of use of aprotin in the repair of ruptured abdominal aortic aneurysm显示文摘 | Robinson J Nawaz S Beard JD | 2000 | Br J Surg2000，12，87： | 1 |
| 14 | Non-destructive testing ofrock bolts using guided ultrasonic waves显示文摘 | BEARD MD LOWE M J S | 2003 | InternationalJournal of Rock Mechanics and Mining Sciences & Ge-omechanics Abstracts2003，40，4： | 1 |
| 15 | On silica activity and serpentinization显示文摘 | Frost B R Beard J S | 2007 | Journal of Petrology2007，48，7： | 1 |
| 16 | Systemic iron status显示文摘 | Beard J Han O | 2009 | Biochim Biophys Acta2009，1790，： | 1 |
| 17 | Antibody response in mice inoculated with DNA expressing foot-and-mouth disease virus capsid proteins显示文摘 | Chinsangaram J Beard C Peter W | 1998 | J Virol1998，72，5： | 1 |
| 18 | Screen for novel cry genes by hybridization显示文摘 | Beard C E Ranasinghe C A Khurst R J | 2001 | Lett Appl Microbiol2001，33，： | 1 |
| 19 | PbTe colloidal nanocrystals: Synthesis, characterization, and multiple exciton generation显示文摘 | Murphy J E Beard M C Norman A G | 2006 | Journal of the American Chemical Society2006，128，10： | 1 |
| 20 | Fast Putting surfaces cause major cultural change显示文摘 | Beard J B | 1998 | Turf A X1998，6，2： | 1 |
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