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| 1 | Serum and urine metabolomic fingerprinting in diagnostics of inflammatory bowel diseases显示文摘AIM:To evaluate the utility of serum and urine metabolomic analysis in diagnosing and monitoring of inflammatory bowel diseases(IBD).METHODS:Serum and urine samples were collected from 24 patients with ulcerative colitis(UC),19 patients with the Crohn’s disease(CD)and 17 healthy controls.The activity of UC was assessed with the Simple Clinical Colitis Activity Index,while the activity of CD was determined using the Harvey-Bradshaw Index.The analysis of serum and urine samples was performed using proton nuclear magnetic resonance(NMR)spectroscopy.All spectra were exported to Matlab for preprocessing which resulted in two data matrixes for serum and urine.Prior to the chemometric analysis,both data sets were unit variance scaled.The differences in metabolite fingerprints were assessed using partial least-squaresdiscriminant analysis(PLS-DA).Receiver operating characteristic curves and area under curves were used to evaluate the quality and prediction performance of the obtained PLS-DA models.Metabolites responsible for separation in models were tested using STATISTICA10 with the Mann-Whitney-Wilcoxon test and the Student’s t test(α=0.05).RESULTS:The comparison between the group of patients with active IBD and the group with IBD in remission provided good PLS-DA models(P value 0.002for serum and 0.003 for urine).The metabolites that allowed to distinguish these groups were:N-acetylated compounds and phenylalanine(up-regulated in serum),low-density lipoproteins and very low-density lipoproteins(decreased in serum)as well as glycine(increased in urine)and acetoacetate(decreased in urine).The significant differences in metabolomic profiles were also found between the group of patients with active IBD and healthy control subjects providing the PLS-DA models with a very good separation(P value<0.001 for serum and 0.003 for urine).The metabolites that were found to be the strongest biomarkers included in this case:leucine,isoleucine,3-hydroxybutyric acid,N-acetylated compounds,acetoacetate,glycine,phenylalanine and lactate(increased in serum),creatine,dimethyl sulfone,histidine,choline and its derivatives(decreased in serum),as well as citrate,hippurate,trigonelline,taurine,succinate and 2-hydroxyisobutyrate(decreased in urine).No clear separation in PLS-DA models was found between CD and UC patients based on the analysis of serum and urine samples,although one metabolite(formate)in univariate statistical analysis was significantly lower in serum of patients with active CD,and two metabolites(alanine and N-acetylated compounds)were significantly higher in serum of patients with CD when comparing jointly patients in the remission and active phase of the diseases.Contrary to the results obtained from the serum samples,the analysis of urine samples allowed to distinguish patients with IBD in remission from healthy control subjects.The metabolites of importance included in this case up-regulated acetoacetate and down-regulated citrate,hippurate,taurine,succinate,glycine,alanine and formate.CONCLUSION:NMR-based metabolomic fingerprinting of serum and urine has the potential to be a useful tool in distinguishing patients with active IBD from those in remission. | Tomasz Dawiskiba Stanislaw Deja Agata Mulak Adam Zabek Ewa Jawień Dorota Pawelka Miroslaw Banasik Agnieszka Mastalerz-Migas Waldemar Balcerzak Krzysztof Kaliszewski Jan Skóra Piotr Bar Krzysztof Korta Kornel Pormańczuk Przemyslaw Szyber Adam Litarski Piotr Mlynarz | 2014 | World Journal of Gastroenterology2014，20，1： | 15 |
| 2 | Clinical and im- munological condition of newborns of mothers treated for recurrent spontaneous abortions with paternal lymphocytes immunization 显示文摘 | Malinowski A Prochowska A Banasik M | 1997 | Eur J Obstet Gynecol Reprod Biol1997，73，1： | 1 |
| 3 | The ivolvement of T lymphoeytes in the pathogenesis of endometriotic tissues overgrowth in women with endometriosis 显示文摘 | Szyllo K Tehorzewski H Banasik M | 2003 | Mediators Inflamm2003，12，3： | 1 |
| 4 | Lymphocyte subset distribution and cytokine secretion in third trimester decidua in normal pregnancy and preeclampsia显示文摘 | Wilczynski JR Tchorzewski H Banasik M | 2003 | Eur J Obstet Gynecol Reprod Biol2003，109，1： | 1 |
| 5 | A cross-platform public domain PC image-analysis program for the comet assay 显示文摘 | Konca K Lankoff A Banasik A | 2003 | Murat Res2003，534，12： | 1 |
| 6 | Four-and-one-half years' experience in monitoring of reproducibility of an MR spectroscopy system-application of in vitro results to interpretation of in vivo data显示文摘 | Skorupa A Wicher M Banasik T | 2014 | Appl Clin Med Phys2014，15，： | 1 |
| 7 | A cross-platform public domain PC image-analysis program for the comet assay 显示文摘 | Konca K Lankoff A Banasik A | 2003 | Mutation Research2003，34，： | 1 |
| 8 | A cross - platform public domain PC image -analysis program for the comet assay显示文摘 | Konca K Lankoff A Banasik A | 2003 | Murat Res2003，534，12： | 1 |
| 9 | Myoglobinufia caused by exertional rhabdomyolysis misdiagnosed as psychiatric illness显示文摘 | Banasik M Kuzniar J Kusztal M | 2008 | Med Sci Monit2008，14，1： | 1 |
| 10 | C4d deposition and positive posttransplant crossmatch are not necessarily markers of antibody-mediated rejection in renal allograft recipients显示文摘 | BANASIK M BORATYNSKA M NOWAKOWSKA B | 2007 | Ransplant Proc2007，39，9： | 1 |
| 11 | The involvement of T lymphocytes in the pathogenesis of endometriotic tissues overgrowth in women with endometriosis显示文摘 | Szyllo K Tchorzewski H Banasik M | 2003 | Mediators Inflamm2003，12，3： | 1 |
| 12 | Lymphocyte subset distribution and cytokine secretion in third trimester decidua in normal pregnancy and preeclampsia显示文摘 | Wilczynski J R Tchorzewski H Banasik M | 2003 | Eur J Obstet Gynecol Reprod Biol2003，109，1： | 1 |
| 13 | The involvement of tlymphocytes in the pathogenesis of endemetriotic tissues overgrowth in women with endometriosis显示文摘 | Tchorzewski H Banasik M | 2003 | Mediators Inflamm2003，12，3： | 1 |
| 14 | Effect of Iyengar yoga practice on fatigue and diurnal salivary cortisol concentration in breast cancer survivors显示文摘 | Banasik J Williams H Haberrnan M | 2011 | J Am Acad Nurse Pract2011，23，3： | 1 |
| 15 | The involvement of T lymphocytes in the pathogenesis of endometriotic tissues overgrowth in women with endometriosis显示文摘 | Szyllo K Tchorzewski H Banasik M | 2003 | Mediators Inflamm2003，12，3： | 1 |
| 16 | Impact of sirolimus treatment in kidney allograft recipients with prolonged cold ischemia times:5-year outcomes显示文摘 | Boratynska M Banasik M Pstrzalek D | 2008 | Exp Clin Transplant2008，6，1： | 1 |
| 17 | Lymphocyte subset distribution and cytokine secretion in third trimester decidua in normal pregnancy and preeclampsia显示文摘 | Wilczynski JR Tchorzewski H Banasik M | 2003 | Eur J Obstet Gynecol Reprod Biol2003，109，1： | 1 |
| 18 | Effect of microcystin- LR and cyanobacterial extract from Polish reservir of drinking water on cell cycle progression , mitotic spindle , and apoptosis in CHO - Kl cells 显示文摘 | LankoffA Banasik A Obe G | 2003 | Toxicology and applied Phamacology2003，189，2： | 1 |
| 19 | Lymphocyte subset distribution and cytokine secretion in third trimester decidua in normal pregnancy and preeclampsia显示文摘 | Wilcznski JR Tchorzewski H Banasik M | 2003 | Eur J Obstet Gynecol2003，109，： | 1 |
| 20 | Long-term follow-up of non-HLA and anti-HLA antibodies: incidence and importance in renal transplantation 显示文摘 | Banasik M Boratynska M Koscielska-Kasprzak K | 2013 | Transplant Proc2013，45，4： | 1 |
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