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| 1 | LIM矿化蛋白-1抑制破骨前体细胞合成一氧化氮的分子机制研究显示文摘目的 探讨LIM矿化蛋白-1(LIM mineralization protein-1,LMp-1)抑制破骨前体细胞合成一氧化氮(nitric oxide,NO)的分子机制.方法 用不同浓度的TAT-LMP-1(0.05nmol/L,0.1 rimol/L,0.25 nmol/L)处理肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)或脂多糖(lipopolysaccharide,LPS)诱导的破骨前体细胞(RAW 264.7细胞),利用Greiss反应间接检测各组NO的生成量.用上述不同浓度的TAT-LMp-1处理LPS诱导的RAW 264.7细胞,使用逆转录-实时定量PCR和Western blot检测细胞中一氧化氮合酶(inducible NO synthase,iNOS)基因及蛋白的表达,利用Western blot检测细胞中NF-κB亚单位P65和磷酸化IκB含量,利用荧光素酶报告基因检测LMp-1对NF-κB通路转录活性的影响.结果 LMP-1可浓度依赖性抑制TNF-α或LPS诱导的RAW 264.7细胞合成NO.LMP-1可浓度依赖性抑制LPS诱导的RAW 264.7细胞中iNOS基因及蛋白的表达、NF-κB亚单位p65和磷酸化IκB含量升高以及NF-κB通路的转录活性.结论 LMp-1抑制LPS诱导的RAW 264.7细胞合成NO的可能分子机制为通过抑制IκB磷酸化使得p65不能与IκB解离,进而阻止p65核转移,导致NF-κB信号通路介导的iNOS生成障碍,最后引起NO生成减少. | 刘辉 Bargouti M 郑召民 张奎渤 Zughaier S 刘云山 Sangadala S Boden SD Titus L | 2011 | 中华骨科杂志2011，31，5： | 3 |
| 2 | The global carbon budget 1959-2011显示文摘 | QuéréC L Andres R J Boden T | 2013 | Earth System Science Data2013，5，： | 1 |
| 3 | The outcome of poskrolater al fusion in highly sekeiod patients with diseogenie low back pain显示文摘 | PARKER L M MU NELL S E BODEN S D | 1996 | Spine1996，21，16： | 1 |
| 4 | Etiology and preven- tion of noneontact ACL injury 显示文摘 | BODEN B GRIFFIN L GARRET W E Jr | 2000 | Phys Sports Med2000，28，4： | 1 |
| 5 | Long- term outcome of second - generation everolimus - eluting stents and Endeavor zotarolimus- eluting stents in a prospec- tive registry o显示文摘 | Velders M A Boden H van der Hoeven B L | 2013 | EuroIntervention2013，8，： | 1 |
| 6 | MEME SUITE: tools for motif discovery and searching显示文摘 | Bailey T L Boden M Buske F A eta! | 2009 | Nucleic Acids Research2009，37，： | 1 |
| 7 | Lumbar spine fusion by local gene therapy with cDNA encoding a novel osteoinduetive protein (LMP-1)显示文摘 | Boden SD Titus L Hair G | 1998 | Spine1998，23，23： | 1 |
| 8 | MEME SUITE: tools for motif discovery and searching 显示文摘 | Bailey T L Boden M Buske F A Frith M Grant C E Clementi L Ren J Li W W Noble W S | 2009 | Nucleic Acids Research2009，37，： | 1 |
| 9 | Lumbar spine fusion by local gene therapy with a cDNA encoding a novel osteoinductive protein(LMP-1)显示文摘 | Boden S D Titus L Hair G | 1998 | Spine1998，23，23： | 1 |
| 10 | New Formulations of demineralized bone matrix as a more effective graft alternative in exprimental posterolateral lumber spine arthodesis显示文摘 | Martin GJ Boden SD Titus L | 1999 | Spine1999，24，7： | 1 |
| 11 | Effect of buffer layer single-event burnout of power DMOSFETs显示文摘 | Liu S Titus J L Boden M | 2007 | IEE] Trans Nucl Sci2007，54，6： | 1 |
| 12 | Does income inequality modify the association between air pol- lution and health显示文摘 | Charafeddine R Boden L | 2008 | Environmental Research2008，106，1： | 1 |
| 13 | HIV-1 drug resistance in newly infeeted individuals显示文摘 | Boden D Hurley A Zhang L | 1999 | JAMA1999，282，12： | 1 |
| 14 | MEME SUITE: tools for motif discovery and searching 显示文摘 | Bailey T L Boden M Buske F A | 2009 | Nucleic Acids Res2009，37，2： | 1 |
| 15 | HIV - 1 drug resistance in newly infected individuals显示文摘 | Boden D Hurley A Zhang L | 1999 | JAMA1999，282，12： | 1 |
| 16 | MEME SUITE: tools for motif discovery and searching 显示文摘 | Bailey T L Boden M Buske F A | 2009 | Nucleic Acids Res2009，37，2： | 1 |
| 17 | Nutritional effcts of fat on carbohydrate metabolism 显示文摘 | Boden G Carnell L FT | 2003 | Clin Endocrinol Metab2003，17，3： | 1 |
| 18 | Relation of Threatened Egotism to Violence and Aggression: the Dark Side of High Selfesteem显示文摘 | BAUMEISTER R SMART L BODEN J | 1996 | Psychological Review1996，103，： | 1 |
| 19 | Oligonucleotide primers for DNA amplification of early regions 1,6 and 7 from human papillomavirus types 6, 11, 16, 18,33显示文摘 | Evander M Boden E Bjersing L | 1991 | Arch Virol1991，253，： | 1 |
| 20 | Etiology and prevention of noneontact ACL injury显示文摘 | Boden B Griffin L Garret W | 2000 | The Physician and Sports Medicine2000，28，1： | 1 |
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