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| 1 | Th1 cytokines promote T-cell binding to antigen-presenting cells via enhanced hyaluronan production and accumulation at the immune synapse显示文摘Hyaluronan(HA)production by dendritic cells(DCs)is known to promote antigen presentation and to augment T-cell activation and proliferation.We hypothesized that pericellular HA can function as intercellular‘glue’directly mediating T cell–DC binding.Using primary human cells,we observed HA-dependent binding between T cells and DCs,which was abrogated upon pre-treatment of the DCs with 4-methylumbelliferone(4-MU),an agent which blocks HA synthesis.Furthermore,T cells regulate HA production by DCs via T cell-derived cytokines in a T helper(Th)subset-specific manner,as demonstrated by the observation that cell-culture supernatants from Th1 but not Th2 clones promote HA production.Similar effects were seen upon the addition of exogenous Th1 cytokines,IL-2,interferon c(IFN-c)and tumor necrosis factor a(TNF-a).The critical factors which determined the extent of DC–T cell binding in this system were the nature of the pre-treatment the DCs received and their capacity to synthesize HA,as T-cell clones which were pre-treated with monensin,added to block cytokine secretion,bound equivalently irrespective of their Th subset.These data support the existence of a feedforward loop wherein T-cell cytokines influence DC production of HA,which in turn affects the extent of DC–T cell binding.We also document the presence of focal deposits of HA at the immune synapse between T-cells and APC and on dendritic processes thought to be important in antigen presentation.These data point to a pivotal role for HA in DC–T cell interactions at the IS. | Paul L Bollyky Stephen P Evanko Rebecca P Wu Susan Potter-Perigo S Alice Long Brian Kinsella Helena Reijonen Kelly Guebtner Brandon Teng Christina K Chan Kathy R Braun John A Gebe Gerald T Nepom Thomas N Wight | 2010 | Cellular & Molecular Immunology2010，7，3： | 3 |
| 2 | Fluorescence and SEM correlative microscopy for nanomanipulation of subcellular structures显示文摘Nanomanipulation under scanning electron microscopy(SEM)enables direct interactions of a tool with a sample.We recently developed a nanomanipulation technique for the extraction and identification of DNA contained within sub-nuclear locations of a single cell nucleus.In nanomanipulation of sub-cellular structures,a key step is to identify targets of interest through correlating fluorescence and SEM images.The DNA extraction task must be conducted with low accelerating voltages resulting in low imaging resolutions.This is imposed by the necessity of preserving the biochemical integrity of the sample.Such poor imaging conditions make the identification of nanometer-sized fiducial marks difficult.This paper presents an affine scale-invariant feature transform(ASIFT)based method for correlating SEM images and fluorescence microscopy images.The performance of the image correlation approach under different noise levels and imaging magnifications was quantitatively evaluated.The optimal mean absolute error(MAE)of correlation results is 68634 nm under standard conditions.Compared with manual correlation by skilled operators,the automated correlation approach demonstrates a speed that is higher by an order of magnitude.With the SEM-fluorescence image correlation approach,targeted DNA was successfully extracted via nanomanipulation under SEM conditions. | Zheng Gong Brandon K Chen Jun Liu Chao Zhou Dave Anchel Xiao Li Ji Ge David P Bazett-Jones Yu Sun | 2014 | Light(Science & Applications)2014，3，1： | 2 |
| 3 | SOFC technology development at Rolls-Royce显示文摘 | Gardner F J Day M J Brandon N P | 2000 | J Power Sources2000，86，： | 2 |
| 4 | Mitochondrial mutations in cancer显示文摘 | Brandon M Baldi P Wallace DC | 2006 | Oncogene2006，25，34： | 1 |
| 5 | Potential benefits of communication in transitive memory system 显示文摘 | HULLINSHEAD A B BRANDON D P | 2003 | Human Communication Research2003，29，： | 1 |
| 6 | Effect of SiC submicrometer particle size and content on frac ture toughness of alumina SiC nanocomposites显示文摘 | Kaplan W P Brandon D G | 1995 | J Am Ceram Soc1995，78，1： | 1 |
| 7 | The direct electro- winning of gold from dilute cyanide leach liquors 显示文摘 | Brandon N P Mahmood M N Page P W | 1987 | Hydrometal- lurgy1987，18，3： | 1 |
| 8 | Modelling of cells,stacks and systems based around metal-supported planar IT-SOFC cells with CGO electrolytes operating at 500 ~ 600 ℃显示文摘 | LEAH R T BRANDON N P AGUIAR P | 2005 | Power Sources2005，145，2： | 1 |
| 9 | Spinning-out a fuel cell com-pany froma UK University-2 years of progress at Ceres Power显示文摘 | Bance P Brandon N P Girvan B | | 0，，： | 1 |
| 10 | Delivery of the Cre recombinase by a self-deleting lentiviral vector:efficient gene targeting in vivo显示文摘 | Pfeifer A Brandon E P Kootstra N | 2001 | Proc Natl Acad Sci USA2001，98，11： | 1 |
| 11 | Micro structure, composition and relationships of plasmasprayed thermal barrier coatings 显示文摘 | TAYLOR R BRANDON R MORRELL P | 1992 | Surface and Costings Technology1992，50，： | 1 |
| 12 | Modeling of ceils, stacks and systems based around metal-supported planar IT-SOFC cells with CGO electrolytes operating at 500-600显示文摘 | Leah R T Brandon N P Aguiar P | 2005 | Journal of Power Sources2005，145，2： | 1 |
| 13 | Com- parison of plasticity in vivo and in vitro in the developing vi- sual cortex of normal and protein kinase A RI/3-deficient mice显示文摘 | HENSCH T K GORDON J A BRANDON E P | 1998 | The Journal of Neuroscience1998，18，6： | 1 |
| 14 | PTPN22 genetic variation: evidence for multiple variants associated with rheumaloid arthritis显示文摘 | Carlton V E Hu X Chokkalingam A P Schrodi S J Brandon R Alexander H C | 2005 | Am J Hum Genet2005，77，： | 1 |
| 15 | The significance of electrochemical impedance spectra recorded during active oxygen evolution for oxide covered Ni, Co and Fe electrodes in alkaline solution 显示文摘 | Lyons M E G Brandon M P | 2009 | Journal of Electroanalytical Chemistry2009，631，： | 1 |
| 16 | Using thermochronology to understand orogenic erosion显示文摘 | Reiners P W Brandon M T | 2006 | Annual Review of Earth and Planetary Sciences2006，34，： | 1 |
| 17 | Anode-supported intermediate temperature direct internal reforming solid oxide fuel cell I:model-based steady state performance显示文摘 | Aguiar P Adjiman C S Brandon N P | 2004 | Journal of Power Sources2004，138，12： | 1 |
| 18 | Influence of Potential on the Friction and Wear of Mild Steel in a Model Aqueous Lubricant显示文摘 | Brandon N P Bonanos N Fogarty P O | 1993 | J Appl Eleetrochem1993，23，： | 1 |
| 19 | Oceanographic variability and changes in Antarctic krill(Euphausia superba)abundance at South Georgia显示文摘 | Trathan P N Brierley A S Brandon M A | 2003 | Fish Oceanogr2003，12，6： | 1 |
| 20 | Development of metal supported solid oxide fuel cells for operation at 500-600℃显示文摘 | Corcoran D Ducker A | 2004 | Jouranl of Materials Engineering and Performance2004，13，3： | 1 |
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