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| 1 | Immunohistochemical expression of SP-NK-1R-EGFR pathway and VDR in colonic inflammation and neoplasia显示文摘AIM:To determine the expression of neurokinin-1receptor(NK-1R),phosphorylated epidermal growth factor receptor(p EGFR),cyclooxygenase-2(Cox-2),and vitamin D receptor(VDR)in normal,inflammatory bowel disease(IBD),and colorectal neoplasia tissues from Puerto Ricans.METHODS:Tissues from patients with IBD,colitisassociated colorectal cancer(CAC),sporadic dysplasia,and sporadic colorectal cancer(CRC),as well as normal controls,were identified at several centers in Puerto Rico.Archival formalin-fixed,paraffin-embedded tissues were de-identified and processed by immunohistochemistry for NK-1R,p EGFR,Cox-2,and VDR.Pictures of representative areas of each tissues diagnosis were taken and scored by three observers using a4-point scale that assessed intensity of staining.Tissues with CAC were further analyzed by photographing representative areas of IBD and the different grades of dysplasia,in addition to the areas of cancer,within each tissue.Differences in the average age between the five patient groups were assessed with one-way analysis of variance and Tukey-Kramer multiple comparisons test.The mean scores for normal tissues and tissues with IBD,dysplasia,CRC,and CAC were calculatedand statistically compared using one-way analysis of variance and Dunnett’s multiple comparisons test.Correlations between protein expression patterns were analyzed with the Pearson’s product-moment correlation coefficient.Data are presented as mean±SE.RESULTS:On average,patients with IBD were younger(34.60±5.81)than normal(63.20±6.13,P<0.01),sporadic dysplasia(68.80±4.42,P<0.01),sporadic cancer(74.80±4.91,P<0.001),and CAC(57.50±5.11,P<0.05)patients.NK-1R in cancer tissue(sporadic CRC,1.73±0.34;CAC,1.57±0.53)and sporadic dysplasia(2.00±0.45)were higher than in normal tissues(0.73±0.19).p EGFR was significantly increased in sporadic CRC(1.53±0.43)and CAC(2.25±0.47)when compared to normal tissue(0.07±0.25,P<0.05,P<0.001,respectively).Cox-2 was significantly increased in sporadic colorectal cancer(2.20±0.23 vs 0.80±0.37 for normal tissues,P<0.05).In comparison to normal(2.80±0.13)and CAC(2.50±0.33)tissues,VDR was significantly decreased in sporadic dysplasia(0.00±0.00,P<0.001 vs normal,P<0.001 vs CAC)and sporadic CRC(0.47±0.23,P<0.001 vs normal,P<0.001 vs CAC).VDR levels negatively correlated with NK-1R(r=-0.48)and p EGFR(r=-0.56)in normal,IBD,sporadic dysplasia and sporadic CRC tissue,but not in CAC.CONCLUSION:Immunohistochemical NK-1R and p EGFR positivity with VDR negativity can be used to identify areas of sporadic colorectal neoplasia.VDR immunoreactivity can distinguish CAC from sporadic cancer. | Raymond A Isidro Myrella L Cruz Angel A Isidro Axel Baez Axel Arroyo William A González-Marqués Carmen González-Keelan Esther A Torres Caroline B Appleyard | 2015 | World Journal of Gastroenterology2015，21，6： | 7 |
| 2 | Confocal laser endomicroscopy and immunoendoscopy for real-time assessment of vascularization in gastrointestinal malignancies显示文摘Gastrointestinal cancers represent a major cause of morbidity and mortality,with incomplete response to chemotherapy in the advanced stages and poor prognosis.Angiogenesis plays a crucial part in tumor growth and metastasis,with most gastrointestinal cancers depending strictly on the development of a new and devoted capillary network.Confocal laser endomicroscopy is a new technology which allows in vivo microscopic analysis of the gastrointestinal mucosa and its microvascularization during ongoing endoscopy by using topically or systemically administered contrast agents.Targeting markers of angiogenesis in association with confocal laser endomicroscopic examination(immunoendoscopy),as a future challenge,will add functional analysis to the morphological aspect of the neoplastic process.This review describes previous experience in endomicroscopic examination of the upper and lower digestive tract with emphasis on vascularization,resulting in a broad spectrum of potential clinical applications,and also preclinical research that could be translated to human studies. | Dan Ionut Gheonea Tatiana Crtnǎ Tudorel Ciurea Carmen Popescu Anca Bǎdǎrǎu Adrian Sǎftoiu | 2011 | World Journal of Gastroenterology2011，17，1： | 6 |
| 3 | Serum vitamin D and colonic vitamin D receptor in inflammatory bowel disease显示文摘AIM: To determine serum vitamin D levels and colonic vitamin D receptor(VDR) expression in inflammatory bowel disease(IBD) and non-IBD patients and correlate these with histopathology.METHODS: Puerto Rican IBD(n = 10) and non-IBD(n = 10) patients ≥ 21 years old scheduled for colonoscopy were recruited. Each patient completed a questionnaire and provided a serum sample and a colonic biopsy of normal-appearing mucosa. For IBD patients, an additional biopsy was collected from visually diseased mucosa. Serum vitamin D levels were measured by ultra-performance liquid chromatography and mass spectrometry. Hematoxylin and eosin stained tissue sections from colonic biopsies were classified histologically as normal or colitis(active/inactive), and scored for the degree of inflammation present(0-3, inactive/absent to severe). Tissue sections from colonic biopsies were also stained by immunohistochemistry for VDR, for which representative diagnostic areas were photographed and scored for staining intensity using a 4-point scale.RESULTS: The IBD cohort was significantly younger(40.40 ± 5.27, P < 0.05) than the non-IBD cohort(56.70 ± 1.64) with a higher prevalence of vitamin D deficiency(40% vs 20%, respectively) and insufficiency(70% vs 50%, respectively). Histologic inflammation was significantly higher in visually diseased mucosa from IBD patients(1.95 ± 0.25) than in normalappearing mucosa from control patients(0.25 ± 0.08, P < 0.01) and from IBD patients(0.65 ± 0.36, P < 0.05) and correlated inversely with VDR expression in visually diseased colonic tissue from IBD patients(r =-0.44, P < 0.05) and from IBD patients with Crohn's disease(r =-0.69, P < 0.05), but not in normal-appearing colonic tissue from control patients or IBD patients. Control and IBD patient serum vitamin D levels correlated positively with VDR expression in normal colon from control and IBD patients(r = 0.38, P < 0.05) and with patient age(r = 0.54, P < 0.01). CONCLUSION: Levels of serum vitamin D correlate positively with colonic VDR expression in visually normal mucosa whereas inflammation correlates negatively with colonic VDR expression in visually diseased mucosa in Puerto Rican patients. | Yamilka Abreu-Delgado Raymond A Isidro Esther A Torres Alexandra González Myrella L Cruz Angel A Isidro Carmen I González-Keelan Priscilla Medero Caroline B Appleyard | 2016 | World Journal of Gastroenterology2016，22，13： | 5 |
| 4 | Pancreaticβ-cell dysfunction in type 2 diabetes:Implications of inflammation and oxidative stress显示文摘Insulin resistance and pancreaticβ-cell dysfunction are major pathological mechanisms implicated in the development and progression of type 2 diabetes(T2D).Beyond the detrimental effects of insulin resistance,inflammation and oxidative stress have emerged as critical features of T2D that defineβ-cell dysfunction.Predominant markers of inflammation such as C-reactive protein,tumor necrosis factor alpha,and interleukin-1βare consistently associated withβ-cell failure in preclinical models and in people with T2D.Similarly,important markers of oxidative stress,such as increased reactive oxygen species and depleted intracellular antioxidants,are consistent with pancreaticβ-cell damage in conditions of T2D.Such effects illustrate a pathological relationship between an abnormal inflammatory response and generation of oxidative stress during the progression of T2D.The current review explores preclinical and clinical research on the pathological implications of inflammation and oxidative stress during the development ofβ-cell dysfunction in T2D.Moreover,important molecular mechanisms and relevant biomarkers involved in this process are discussed to divulge a pathological link between inflammation and oxidative stress duringβ-cell failure in T2D.Underpinning the clinical relevance of the review,a systematic analysis of evidence from randomized controlled trials is covered,on the potential therapeutic effects of some commonly used antidiabetic agents in modulating inflammatory makers to improveβ-cell function. | Phiwayinkosi V Dludla Sihle E Mabhida Khanyisani Ziqubu Bongani B Nkambule Sithandiwe E Mazibuko-Mbeje Sidney Hanser Albert Kotze Basson Carmen Pheiffer Andre Pascal Kengne | 2023 | World Journal of Diabetes2023，14，3： | 2 |
| 5 | Intravitreal triamcinolone for refractory diabetic macular edema显示文摘 | Adam Martidis Jay S Duker Paul B Greenberg Adam H Rogers Carmen A Puliafito Elias Reichel Caroline Baumal | 2002 | Ophthalmology2002，，5： | 2 |
| 6 | Membrane microparticles:two sides of the coin显示文摘 | Hugel B Carmen M Martinez MC | 2005 | Physiology2005，20，1： | 1 |
| 7 | Sonolysis of chlorobenzene in fenton-type aqueous system显示文摘 | Carmen Stavarache Yim B | 2002 | Ultrasonics Sonochemistry2002，，9： | 1 |
| 8 | The results of open reduction and internal fixation of distal femur fractures using a biologic (indirect) reduction technique显示文摘 | Bolhofner BR Carmen B Clifford P | 1996 | J Orthop Trauma1996，10，6： | 1 |
| 9 | Molecular characterization and expression of alfalfa ( Medicago sativa L.) flavanone-3-hydroxylase and dihydroflavonol-4-reductase encoding genes显示文摘 | Bénédicte Charrier Carmen Coronado Adam Kondorosi Pascal Ratet | 1995 | Plant Molecular Biology1995，，4： | 1 |
| 10 | Angiotension Ⅱ participates in mononuclear cell recruitment in experimental immune complex nephritis through nuclear factor-κB activation and monocyte chemoattractant protein-1 synthesis显示文摘 | Marta RO Carmen B Moguel A | 1998 | J Immunol1998，161，1： | 1 |
| 11 | The combination of ischemic preconditioning and live Bcl-2 overexpression is a suitable strategy to prevent liver and Lung Damage after Hepatic Ischemia-reperfusion显示文摘 | Carmen P Jose CP Ramon B | 2002 | Am J Pathol2002，160，6： | 1 |
| 12 | Defined culture conditions of human embryonic stem cells line 显示文摘 | LU J HOU R H CARMEN J B | 2006 | Proc Natl Acad Sci USA2006，103，15： | 1 |
| 13 | The results of open reduction and internal fixation of distal femur fractures using a biologic (indirect) reduction technique显示文摘 | Bolhofner B R Carmen B Clifford P | 1996 | J Orthop Trauma1996，10，： | 1 |
| 14 | Hemorrhagic transformation in cardioembolic cerebral infarction 显示文摘 | Claus H Thomas B Carmen S | 1993 | Stroke1993，24，2： | 1 |
| 15 | Flame retarding of wood by impregnation with boric acid--Pyrolysis products and char oxidation rates显示文摘 | Colomba D B Carmen B Antonio G | 2007 | Polymer Degradation and Stability2007，92，： | 1 |
| 16 | Control of Filament Formation in Candida albicans by Polyamine Levels 显示文摘 | Ana B Herrero M Carmen Lo'pez | 1999 | lnfec Immunity1999，67，9： | 1 |
| 17 | Lean phenotype and resist- ance to diet-induced obesity in vitamin D receptor knockout mice correlates with induction of uncoupling protein-1 in white adipose tissue显示文摘 | Carmen JN Donald M Emily B | 2009 | Endocrinol2009，150，2： | 1 |
| 18 | Effect of lead on ALA-D activi- ty, metallothionein le'/elg and lipid peroxidation in blood, kidney and liver of the toadfish Halobatrachus didactylus 显示文摘 | O Carmen S Julian B | 2003 | Ecotox Envi- ron Safe2003，55，1： | 1 |
| 19 | Hemorrhagic transformation in cardioembolic cerebral infarction显示文摘 | Thomas B Carmen S | 1993 | Stroke1993，24，： | 1 |
| 20 | Legionella pneumophila adaptation to intracellular life and the host response: Clues from ge nomics and transcriptomics显示文摘 | Matthieu J Carmen B | 2007 | FEBS Letters2007，581，15： | 1 |
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