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| 1 | Neural stem cell markers,nestin and musashi proteins,in the progression of human glioma:correlation of nestin with prognosis of patient survival显示文摘 | Strojnik T R■sland GV Sakariassen PO Kavalat R Lab T | 2007 | 中国神经肿瘤杂志2007，5，3： | 34 |
| 2 | K-ATP channel openers facilitate glutamate uptake by GluTs in rat primary cultured Astrocytes显示文摘Increasing evidence, including from our laboratory, has revealed that opening of ATP sensitive potassium channels(K-ATP channels) plays the neuronal protective roles both in vivo and in vitro. Thus K-ATP channel openers(KCOs) have been proposed as potential neuroprotectants. Our previous studies demonstrated that K-ATP channels could regulate glutamate uptake activity in PC12 cells as well as in synaptosomes of rats. Since glutamate transporters(GluTs) of astrocytes play crucial roles in glutamate uptake and KATP channels are also expressed in astrocytes, the present study showed whether and how KATP channels regulated the function of GluTs in primary cultured astrocytes. The results showed that nonselective KCO pinacidil, selective mitochondrial KCO diazoxide, novel, and blood-brain barrier permeable KCO iptakalim could enhance glutamate uptake, except for the sarcolemmal KCO P1075. Moreover pinacidil, diazoxide, and iptakalim reversed the inhibition of glutamate uptake induced by 1-methyl-4-phenylpyridinium(MPP+). These potentiated effects were completely abolished by mitochondrial K-ATP blocker 5-hydroxydecanoate. Furthermore, either diazoxide or iptakalim could inhibit MPP+-induced elevation of reactive oxygen species (ROS) and phosphorylation of protein kinases C(PKC). These findings are the first to demonstrate that activation of K-ATP channel, especially mitochondrial K-ATP channel, improves the function of GluTs in astrocytes due to reducing ROS production and downregulating PKC phosphorylation. Therefore, the present study not only reveals a novel pharmacological profile of KCOs as regulators of GluTs, but also provides a new strategy for neuroprotection. | Sun, X. L. Zeng, X. N. Zhou, F. Dai, C. P. Ding, J. H. Hu, G. Nanjing Med Univ,Dept Anat Histol & Pharmacol,Neuropharmacol Lab,Nanjing 210029,Jiangsu,Peoples R China. | 2008 | 南京医科大学学报(自然科学版)2008，28，6： | 7 |
| 3 | Pathological and biochemical alterations of astrocytes in ovariectomized rats injected with D-galactose:A potential contribution to Alzheimer's disease processes显示文摘Astrocytes are implicated in the pathological changes of Alzheimer's disease. Our previous studies have demonstrated that estrogen deprivation and oxidative stress act synergistically to accelerate the progress of Alzheimer's disease. Long-term D-galactose injection combined with ovariectomy may serve as a rodent model for Alzheimer's disease. To address the potential contribution of astroglia to the Alzheimer's disease pathogenesis, we investigated pathological and biochemical alterations of astrocytes under this animal model. Ovadectomized rats injected with D-galactose for 2 weeks showed extensive localization of glial fibrillary acidic protein immunoreactive astrocytes and slightly elevated glutathione levels in the hippocampus without significant impairments in the water maze test and deficits of the cholinergic analyses, compared to the saline-injected rats. Ovariectomized rats injected with D-galactose for 6 weeks, however, exhibited degeneration of astrocytes and decreased glutathione levels in the hippocampus, accompanied with severe dysfunction of behavioral test and deficiency of cholinergic terminals. Electron microscopy further confirmed the pathological changes of astrocytes, especially in the aggregated area of synapse and brain microvessels. Consistent with degeneration of perivascular astrocytic endfeet, analysis of the horseradish peroxidase demonstrated an impairment of the blood-brain barrier permeability. These findings indicate that biochemical and pathological alterations of astrocytes may partially contribute to exacerbating neuronal deficits in the course of Alzheimer's disease. Restoring neuroprotective potential of astrocytes may be a useful therapeutic target for Alzheimer's disease and other neurodegenerative diseases. | Hua, X. D. Lei, M. Ding, H. Han, Q. Y. Hu, G. Xiao, M. Nanjing Med Univ,Dept Anat Histol & Pharmacol,Jiangsu Prov Key Lab Neurodegenerat,Nanjing 210029,Peoples R China. | 2008 | 南京医科大学学报(自然科学版)2008，28，6： | 4 |
| 4 | Complieation, reopera- tion rates, and health - care cost following surgical treatment of lumbar spondylolisthesis 显示文摘 | LAB SP BABU R BAKER AA | 2013 | J Bone Joint Surg Am2013，95，21： | 1 |
| 5 | The Integrated Model of Embedded Management Systems and Its Implementation显示文摘The Simple Network Management Protocol( SNMP ) is nowadays the key enabling management technology, while Web technologies have proved to be attractive to networks and systems management. Future development in the management domain should be 'integrated'. In this article, an embedded management model, which provides an integrated device management framework, is presented. This model consists of five functional modules including System service layer, Application layer, Data layer, Middle layer and Access layer. Each module is described, with the mutual relation included. Then the key points of implementation are discussed. And the system design with the development tools provided by WindRiver Systems, which enable products to be developed cost effectively and efficiently, is described in detail. | LIU Hong, DING Wei (Broadband Communication Network R&D Lab, Beijing University of Posts and Telecommunications, Beijing 100876, P.R.China) | 2002 | The Journal of China Universities of Posts and Telecommunications2002，9，3： | 1 |
| 6 | Beckwith - Wiedemann syndrome and assisted reproduction technology (ART) 显示文摘 | E R Maher LAB S C Bowdin A Luharia W Cooper T R Cole F Macdonald | 2003 | J Med Genet2003，40，： | 1 |
| 7 | The Inflamma- some-mediated caspase-1 activation controls adipocyte differ- entiation and insulin sensitivity 显示文摘 | Stienstra R Joosten LAB Koenen T | 2010 | Cell Metab2010，12，： | 1 |
| 8 | Assess ment of collateral perfusion:a pharmacodynamic study with buflo medil hydrochloride显示文摘 | Labs KH Eichlis berger R Jeanneret C | 2000 | Angiology2000，51，4： | 1 |
| 9 | Relationship between accident investigations, risk analysis, and safety management显示文摘 | LABS H R | 2004 | Journal of Hazardous Materials2004，111，13： | 1 |
| 10 | Evaluation of the synthesis of sialic acid-PAMAM glycodendrimers without the use of sugar protecting groups,and the anti-HIV-1 properties of these compounds显示文摘 | Clayton R Hardman J LaB ranche CC | 2011 | Bioconjug Chem2011，22，10： | 1 |
| 11 | Assess ment of collateral perfusion: a pharmacodynamic study with butlomedil hydrochloride 显示文摘 | Labs KH Eichlis berger R Jeanneret C | 2000 | Angiology2000，51，4： | 1 |
| 12 | Assessment of collateral perfusion: a pharmacodynamic study with buflomedil hydrochloride显示文摘 | Labs KH Eichlisberger R Jeanneret C | 2000 | Angiology2000，51，4： | 1 |
| 13 | A tutorial on hidden Markov models and selected applications in speech recognition显示文摘 | Rabiner L R AT&T Bell Lab Murray Hill | 1989 | Proceedings of the IEEE1989，77，2： | 1 |
| 14 | Tissue inhibitor of metalloproteinase-1 protects MCF-7 breast cancer cells from paclitaxel-induced apoptosis by decreasing the stability of cyclin B1显示文摘Paclitaxel(PTX) is a very effective drug in treating tumors.It disturbs microtubule dynamics and impairs the transition of cells from metaphase to anaphase in mitosis,leading to cell death by apoptosis.However,the effectiveness of PTX in cancer chemotherapy is hampered by drug resistance in some patients.Tissue inhibitor of metalloproteinase-1(TIMP-1) is well known to be capable of inhibiting apoptosis.Elevated tumor tissue TIMP-1 levels have been significantly associated with a poor response to chemotherapy.We hypothesized that TIMP-1 could reduce the sensitivity of breast cancer cells to PTX by inhibiting apoptosis.To test this hypothesis,we first examined the effects of TIMP-1 on the apoptosis induced by PTX and investigated the effects of TIMP-1 on the expression and stability of cyclin B1 that critically regulates the metaphase to anaphase transition during mitosis in MCF-7 breast cancer cells.Our data demonstrate that TIMP-1 could significantly decrease the sensitivity of MCF-7 cells to PTX-induced apoptosis,attenuate mitotic blockage in G(2) /M,and enhance the degradation of cyclin B1.To further investigate whether the inhibitory effect of TIMP-1 on PTX-induced apoptosis is mediated by lowering levels of cyclin B1,a cyclin B1-expression plasmid was transfected into clone overexpressing TIMP-1.The levels of PTX-induced apoptosis were then analyzed.The data showed that the TIMP-1-based decrease in PTX-induced apoptosis was reversed by cyclin B1.Our data indicate that TIMP-1 can protect breast cancer cells from PTX-induced apoptosis by decreasing the stability of cyclin B1. | Wang,T Lv,JH Zhang,XF Li,CJ Han,X Sun,YJ Nanjing Med Univ,Key Lab Human Funct Genom Jiangsu Prov,Nanjing 210029,Peoples R China Nanjing Med Univ,Dept Cell Biol & Med Genet,Nanjing 210029,Peoples R China Nanjing Univ,Sch Med,Nanjing 210008,Peoples R China Nanjing Med Univ,Ctr Canc,Nanjing 210029,Peoples R China | 2010 | 南京医科大学学报(自然科学版)2010，30，5： | 1 |
| 15 | Assessment of collateral perfusion: a pharmaeodynamic study with buflomedil hydroehloride显示文摘 | Labs KH Eiehlisberger R Jeanneret C | 2000 | Angiology2000，51，4： | 1 |
| 16 | In Vivo Antimicrobial Activity of 2% Chlorhexidine Used as a Root Canal Irrigating Solution 显示文摘 | Leonardo M R Filho M T Silva LAB | 1999 | Journal of Endodontics1999，25，3： | 1 |
| 17 | Assessment of collateral perfusion: a pharmacodynamic study with builomedil hydrochloride显示文摘 | Labs KH Eichlisberger R Jeanneret C | 2000 | Angiology2000，51，4： | 1 |
| 18 | A review of the adverse events profile of cefpirome显示文摘 | Rubinstein E Labs R Reeves A | 1993 | Drug Safety1993，9，5： | 1 |
| 19 | Human immunodeficiency virus type 1 tat accelerates kaposi sarcoma-associated herpesvirus kaposin A-mediated tumorigenesis of transformed fibroblasts in vitro as well as in nude and immunocompetent mice显示文摘Kaposi sarcoma-associated herpesvirus(KSHV) is necessary but not sufficient to cause Kaposi sarcoma(KS).Coinfection with human immunodeficiency virus type 1(HIV-1), in the absence of antiretroviral suppressive therapy, drastically increases the risk of KS.Previously, we identified that HIV-1 transactivative transcription protein(Tat) was an important cofactor that activated lytic cycle replication of KSHV.Here, we further investigated the potential of Tat to influence tumorigenesis induced by KSHV Kaposin A, a product of KSHV that was encoded by the open reading frame K12(a KSHV-transforming gene).By using colony formation in soft agar, H-3-TdR incorporation, cell cycle, and microarray gene expression analyses, we demonstrated that Tat enhanced proliferation as well as mitogen-activated protein kinase, signal transducer and activator of transcription 3, and phosphatidylinositol 3-kinase/protein kinase B signaling induced by Kaposin A in NIH3T3 cells.Animal experiments further demonstrated that Tat accelerated tumorigenesis by Kaposin A in athymic nu/nu mice.Cells obtained from primary tumors of nude mice succeeded inducing tumors in immunocompetent mice.These data suggest that Tat can accelerate tumorigenesis induced by Kaposin A.Our data present the first line of evidence that Tat may participate in KS pathogenesis by collaborating with Kaposin A in acquired immunodeficiency syndrome(AIDS)-related KS(AIDS-KS) patients.Our data also suggest that the model for Kaposin and Tat-mediated oncogenesis will contribute to our understanding of the pathogenesis of AIDS-KS at the molecular level and may even be important in exploring a novel therapeutic method for AIDS-KS. | Chen, Xiuying Cheng, Lin Jia, Xuemei Yao, Shuihong Lv, Zhigang Qin, Di Fang, Xin Lu, Chun Nanjing Med Univ, Dept Microbiol & Immunol, Nanjing 210029, Peoples R China.Chen, Xiuying Lu, Chun Nanjing Med Univ, Lab Reprod Med, Nanjing 210029, Peoples R China.Chen, Xiuying Lu, Chun Nanjing Med Univ, Key Lab Pathogen Biol Jiangsu Prov, Nanjing 210029, Peoples R China.Chen, Xiuying Lu, Chun Key Lab Lab Med Jiangsu Prov, Nanjing 210029, Peoples R China.Chen, Xiuying Lei, Yongliang Lishui Ctr Dis Control & Prevent, Lishui 323000, Zhejiang, Peoples R China.Cheng, Lin Huanghe Sci & Technol Coll, Dept Microbiol & Immunol, Zhengzhou 450006, Peoples R China.Zeng, Yi Youjiang Med Coll Nationalities, Dept Microbiol & Immunol, Bose 533000, Peoples R China. | 2010 | 南京医科大学学报(自然科学版)2010，30，1： | 1 |
| 20 | The Distributed Gateway Interworking with SIP/H.323显示文摘The brief description and comparison are given to the following two signaling protocol standards that are currently used in Internet: ITU T Recommendation H .323 and IETF Session Initiation Protocol ( SIP ). After the introduction to the latest Distributed Gateway ( DG ) architecture and its internal protocol Media Gateway Control Protocol ( MGCP ), we describe a framework for DG interworking with H.323/SIP . Other topics, such as address translation, relocation of signaling transport protocol architecture and adding components to DG for interworking with H.323/SIP , are also addressed. | XU Zhan qi 1,\ CAO Xi ren 2,\ LI Bo 2,\ FANG Qiang 3 Foundation item: This work was supported by HK contract CRC98/01.EG03. Biography:\ XU Zhan qi(1962-), male, associate professor, National Key Lab on ISN, Xidian University, interested in the r | 2001 | The Journal of China Universities of Posts and Telecommunications2001，8，1： | 0 |
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