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| 1 | Effect of adipose-derived mesenchymal stem cells on hepatocellular carcinoma: In vitro inhibition of carcinogenesis显示文摘AIM To investigate the effect of adipose-derived mesenchymal stem cells(ADMSCs)and their conditioned media(CM) on hepatocellular carcinoma(HCC) cell tumorigenesis.METHODS The proliferation rate of HepG2 and PLC-PRF-5 HCC cancer cells was measured using the trypan blue exclusion method and confirmed using the cell-counting kit8(commonly known as CCK-8) assay. Apoptosis was detected by flow cytometry using annexin V-FITC. Protein and mRNA expression was quantified by ELISA and real time PCR, respectively. Migration and invasion rates were performed by Transwell migration and invasion assays. Wound healing was examined to confirm the data obtained from the migration assays.RESULTS Our data demonstrated that when co-culturing HCC cell lines with ADMSCs or treating them with ADMSC CM, the HCC cell proliferation rate was significantly inhibited and the apoptosis rate increased. The decreased proliferation rate was accompanied by an upregulation of P53 and Retinoblastoma mRNA and a downregulation of c-Myc and hTERT mRNA levels. More notably, ADMSCs and their CM suppressed the expression of the two important markers of HCC carcinogenicity, alpha-fetoprotein and Des-gamma-carboxyprothrombin. In addition, the migration and invasion levels of HepG2 and PLC-PRF-5 cells significantly decreased, potentially through increased expression of the tissue inhibitor metalloproteinases TIMP-1, TIMP-2 and TIMP-3.CONCLUSION These findings shed new light on a protective and therapeutic role for ADMSCs and their CM in controlling HCC invasiveness and carcinogenesis. | Rim Serhal Nagib Saliba George Hilal Mayssam Moussa Ghada S Hassan Oula El Atat Nada Alaaeddine | 2019 | World Journal of Gastroenterology2019，25，5： | 6 |
| 2 | Primart structure of the aequorea victeria green-fluoresentprotein显示文摘 | Oulas CP Virginia K William W | 1992 | Gene1992，111，2： | 1 |
| 3 | MicroRNAs and cancer-the search begins 显示文摘 | Oulas A Reczko M Poirazi P | 2009 | IEEE Trans Inf Technol Biomed2009，13，1： | 1 |
| 4 | MicroRNAs and cancer the search begins 显示文摘 | Oulas A Reczko M Poirazi P | 2009 | IEEE Trans InfTechnol Biomed2009，13，1： | 1 |
| 5 | C4 photosynthesis and water stress 显示文摘 | Oula G | 2009 | Annals of Botany2009，103，4： | 1 |
| 6 | MicroRNAs and cancer-the search begins 显示文摘 | Oulas A Reczko M Poirazi P | 2009 | IEEE Trans lnf Technol Biomed2009，13，1： | 1 |
| 7 | MicroRNAs and cancer-the search begins! 显示文摘 | Oulas A Reczko M Poirazi P | 2009 | IEEE Trans Inf Yechnol Biomed2009，13，1： | 1 |
| 8 | Diagnostic ability and limitation of ERCP for pancreatic cancer显示文摘 | Takagi K Oula H Ohhashi I | | 0，，： | 1 |
| 9 | MieroRNAs and cancer - the search begins显示文摘 | Oulas A Reczko M Poirazi P | 2009 | IEEE Trans Inf Technol Biomed2009，13，1： | 1 |
| 10 | C4 photosynthesis and water stress显示文摘 | Oula Ghannoum | 2009 | Annals of Botany2009，103，4： | 1 |
| 11 | Water sorption isotherms and glass transition temperature of spray dried tomato pulp显示文摘 | OULA A M KARAPANTSIOS T D ACHILIAS D S | 2008 | Journal of Food Engineering2008，85，1： | 1 |
| 12 | Diagnostic ability and limitation of ERCP for pancreatic cancer显示文摘 | Takagi K Oula H ohhashi l | 1982 | Stomach intestine1982，17，10： | 1 |
| 13 | MicroRNAs and cancer the search begins! 显示文摘 | Oulas A Reczko M Poirazi P | 2009 | IEEE Trans lnf Technol Bi- omed2009，13，1： | 1 |
| 14 | Proto Kranz-like leaf traits and cellular ionic regulation are associated with salinity tolerance in a halophytic wild rice显示文摘Species of wild rice(Oryza spp.)possess a wide range of stress tolerance traits that can be potentially utilized in breeding climate-resilient cultivated rice cultivars(Oryza sativa)thereby aiding global food security.In this study,we conducted a greenhouse trial to evaluate the salinity tolerance of six wild rice species,one cultivated rice cultivar(IR64)and one landrace(Pokkali)using a range of electrophysiological,imaging,and whole-plant physiological techniques.Three wild species(O.latifolia,O.officinalis and O.coarctata)were found to possess superior salinity stress tolerance.The underlying mechanisms,however,were strikingly different.Na+accumulation in leaves of O.latifolia,O.officinalis and O.coarctata were significantly higher than the tolerant landrace,Pokkali.Na+accumulation in mesophyll cells was only observed in O.coarctata,suggesting that O.officinalis and O.latifolia avoid Na+accumulation in mesophyll by allocating Na+to other parts of the leaf.The finding also suggests that O.coarctata might be able to employ Na+as osmolyte without affecting its growth.Further study of Na+allocation in leaves will be helpful to understand the mechanisms of Na+accumulation in these species.In addition,O.coarctata showed Proto Kranz-like leaf anatomy(enlarged bundle sheath cells and lower numbers of mesophyll cells),and higher expression of C4-related genes(e.g.,NADPME,PPDK)and was a clear outlier with respect to salinity tolerance among the studied wild and cultivated Oryza species.The unique phylogenetic relationship of O.coarctata with C4 grasses suggests the potential of this species for breeding rice with high photosynthetic rate under salinity stress in the future. | Miing-Tiem Yong Celymar Angela Solis Samuel Amatoury Gothandapani Sellamuthu Raja Rajakani Michelle Mak Gayatri Venkataraman Lana Shabala Meixue Zhou Oula Ghannoum Paul Holford Samsul Huda Sergey Shabala Zhong-Hua Chen | 2022 | Stress Biology2022，2，1： | 0 |
| 15 | Planning urban megaprojects in the Gulf: The international consultancy firms in urban planning between global and contingent显示文摘 | Oula Aoun Jacques Teller | 2016 | Frontiers of Architectural Research2016，5，2： | 0 |
| 16 | Hierarchical Mean-Field Type Control of Price Dynamics for Electricity in Smart Grid显示文摘This paper solves a mean-field type hierarchical optimal control problem in electricity market.The authors consider n-1 prosumers and one producer.The ith prosumer,for 1 | FRIHI Zahrate El Oula CHOUTRI Salah Eddine BARREIRO-GOMEZ Julian TEMBINE Hamidou | 2022 | Journal of Systems Science & Complexity2022，35，1： | 0 |
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