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| 1 | Overexpression of p27^(KIP1)induced cell cycle arrest in G_1 phase and subsequent apoptosis in HCC-9204 cell line显示文摘AIM We have previously reported that inducibleover-expression of Bak may prolong cell cycle inG1 phase and lead to apoptosis in HCC-9204 cells.This study is to investigate whether p27KIP1playsan important role in this process.METHODS In order to elucidate the exactfunction of p27KIP1in this process,a zinc induciblep27KIP1stable transfectant and transient p27KIP1-GFP fusion transfectant were constructed.Theeffects of inducible,p27KIP1on cell growth,cellcycle arrest and apoptosis were examined in themock,control pMD vector,and pMD-KIP1transfected HCC-9204 cells.RESULTS This p27KIP1-GFP transfectant maytransiently express the fusion gene.The cellgrowth was reduced by 35% at 48 h of p27KIP1induction with zinc treatment as determined bytrypan blue exclusion assay.These differencesremained the same after 72 h of p27KIP1expression,p27KIP1caused cell cycle arrest after24 h of induction,with 40% increase in G1population.Prolonged p27KIP1expression in thiscell line induced apoptotic cell death reflected byTUNEL assay.Fourty-eight h and 72 h of p27KIP1expression showed a characteristic DNA ladder onagarose gel electrophoresis. CONCLUSION Bak may induce cell cycle arrest inG1 phase through upregulating expression ofp27KIP1and subsequently lead to apoptosis inHCC-9204 cells.The p27KIP1-GFP fusion proteincan be transiently expressed in HCC-9204 cells.The inducible p27KIP1-expressing cell line providesa model to assess p27KIP1function. | Jiang Li Xin Ke Yang Xin Xin Yu Meng Liang Ge Wen Liang Wang Jie Zhang Yun De Hou Department of Pathology,Fourth Military Medical University,Xi’an 710033,Shaanxi Province,China State Key Laboratory for Molecular Virology and Genetic Engineering,Beijing 100052,China Department of Dermatology,Beijing Hospital,Beijing 100016,China Institute of Radiation Medicine,Beijing 100085,China | 2000 | World Journal of Gastroenterology2000，6，4： | 20 |
| 2 | The enhanced X-ray Timing and Polarimetry mission—eXTP显示文摘In this paper we present the enhanced X-ray Timing and Polarimetry mission—eXTP. eXTP is a space science mission designed to study fundamental physics under extreme conditions of density, gravity and magnetism. The mission aims at determining the equation of state of matter at supra-nuclear density, measuring effects of QED, and understanding the dynamics of matter in strong-field gravity. In addition to investigating fundamental physics, eXTP will be a very powerful observatory for astrophysics that will provide observations of unprecedented quality on a variety of galactic and extragalactic objects. In particular, its wide field monitoring capabilities will be highly instrumental to detect the electro-magnetic counterparts of gravitational wave sources.The paper provides a detailed description of:(1) the technological and technical aspects, and the expected performance of the instruments of the scientific payload;(2) the elements and functions of the mission, from the spacecraft to the ground segment. | ShuangNan Zhang Andrea Santangelo Marco Feroci YuPeng Xu FangJun Lu Yong Chen Hua Feng Shu Zhang Sφren Brandt Margarita Hernanz Luca Baldini Enrico Bozzo Riccardo Campana Alessandra De Rosa YongWei Dong Yuri Evangelista Vladimir Karas Norbert Meidinger Aline Meuris Kirpal Nandra Teng Pan Giovanni Pareschi Piotr Orleanski QiuShi Huang Stephane Schanne Giorgia Sironi Daniele Spiga Jiri Svoboda Gianpiero Tagliaferri Christoph Tenzer Andrea Vacchi Silvia Zane Dave Walton ZhanShan Wang Berend Winter Xin Wu Jean J.M.in't Zand Mahdi Ahangarianabhari Giovanni Ambrosi Filippo Ambrosino Marco Barbera Stefano Basso Jörg Bayer Ronaldo Bellazzini Pierluigi Bellutti Bruna Bertucci Giuseppe Bertuccio Giacomo Borghi XueLei Cao Franck Cadoux Francesco Ceraudo TianXiang Chen Yu Peng Chen Jerome Chevenez Marta Civitani Wei Cui WeiWei Cui Thomas Dauser Ettore Del Monte Sergio Di Cosimo Sebastian Diebold Victor Doroshenko Michal Dovciak YuanYuan Du Lorenzo Ducci QingMei Fan Yannick Favre Fabio Fuschino JoséLuis Ga'lvez Min Gao MingYu Ge Olivier Gevin Marco Grassi QuanYing Gu YuDong Gu DaWei Han Bin Hong Wei Hu Long Ji ShuMei Jia WeiChun Jiang Thomas Kennedy Ingo Kreykenbohm Irfan Kuvvetli Claudio Labanti Luca Latronico Gang Li MaoShun Li Xian Li Wei Li ZhengWei Li Olivier Limousin HongWei Liu XiaoJing Liu Bo Lu Tao Luo Daniele Macera Piero Malcovati Adrian Martindale Malgorzata Michalska Bin Meng Massimo Minuti Alfredo Morbidini Fabio Muleri Stephane Paltani Emanuele Perinati Antonino Picciotto Claudio Piemonte JinLu Qu Alexandre Rachevski Irina Rashevskaya Jerome Rodriguez Thomas Schanz ZhengXiang Shen LiZhi Sheng JiangBo Song LiMing Song Carmelo Sgro Liang Sun Ying Tan Phil Uttley Bo Wang DianLong Wang GuoFeng Wang Juan Wang LangPing Wang YuSa Wang Anna L.Watts XiangYang Wen Jörn Wilms ShaoLin Xiong JiaWei Yang Sheng Yang YanJi Yang Nian Yu WenDa Zhang Gianluigi Zampa Nicola Zampa Andrzej A.Zdziarski AiMei Zhang ChengMo Zhang Fan Zhang Long Zhang Tong Zhang Yi Zhang XiaoLi Zhang ZiLiang Zhang BaoSheng Zhao ShiJie Zheng Yu Peng Zhou Nicola Zorzi J.Frans Zwart | 2019 | Science China(Physics,Mechanics & Astronomy)2019，62，2： | 11 |
| 3 | Removal of adhesive dusts from flue gas using corona discharges with spraying water显示文摘Effective removal of adhesive and fine dusts from flue gas is very difficult. A new method of electrostatic precipitation of the corona discharges with spraying water(CDSW) was introduced. A new electrode configuration and the circulation spraying of water were employed in the method. The efficient electrostatic precipitation for adhesive and fine dusts can be accomplished without any drain water during a long operating period. The fundamental structure, discharge characteristics, mechanism of spraying and precipitation principle of the electrostatic precipitation using CDSW were described and analyzed. The V I characteristics, spraying state, supplying water quantity, influence of temperature and clean of the electrodes were researched in series experiments. The treating effects of circulating spraying using the corona plasma at the same time of electrostatic precipitation were investigated. The fundamental theories and experimental data were proposed, in order to effectively remove the adhesive dusts from flue gas using CDSW in practice. | XU De\|xuan, ZHAO Jian\|wei, DING Yun\|zheng, GE Wei\|li (Department of Environmental Science and Engineering, Northeast Normal University, Changchun 130024, China. | 2003 | Journal of Environmental Sciences2003，15，4： | 11 |
| 4 | Mycoplasma pneumoniae Macrolide Resistance and MLVA Typing in Children in Beijing,China,in 2016:Is It Relevant?显示文摘Objective The aim of this study is to investigate the macrolide resistance rate and molecular type with multiple-locus variable-number tandem-repeat analysis(MLVA)of Mycoplasma pneumoniae of Beijing in 2016 in pediatric patients.Methods Real-time quantitative polymerase chain reaction(PCR)was used to identify M.pneumoniae,and MLVA was performed.The domain V of the 23 S rRNA was sequenced to detect macrolide-resistant point mutations.We also investigated the activities of antibiotics against M.pneumoniae isolates in vitro.Results The PCR detection rate of M.pneumoniae in children in Beijing was 40%,and the macrolide resistance rate was 66%.The A2063 G mutation in the 23 S rRNA V region is the dominant mutation(137/146,93.84%),whereas the A2064 G mutation is rare(9/146,6.16%).Seventy-three samples were typed successfully by MLVA typing,including 86.3%(63/73)were MLVA type 4-5-7-2,and 13.7%(10/73)were MLVA type 3-5-6-2.No other types were found.No strains were resistant to levofloxacin or tetracycline.Conclusion In 2016,a specific decrease in the macrolide resistance rate occurred in Beijing.The detection rate and macrolide resistance rate of outpatients are lower than those of inpatients.The A2063 G mutants M.pneumoniae have high levels of resistance to erythromycin and azithromycin.The primary MLVA type is 4-5-7-2,followed by 3-5-6-2.No other MLVA types were detected.No strains resistant to tetracycline or levofloxacin were found in vitro. | DOU Hai Wei TIAN Xiu Jun XIN De Li WEI Ran ZHOU Wei WANG Hong QIN Xuan Guang SHAO Jun Yan XU Bao Ping GE Li Xia SHI Da Wei | 2020 | Biomedical and Environmental Sciences2020，33，12： | 9 |
| 5 | Prophylactic Use of Transjugular Intrahepatic Portosystemic Shunt Aids in the Treatment of Refractory Ascites: Metaregression and Trial Sequential Meta-analysis显示文摘 | Ren Pin Chen Xiao Ju Zhu Ge Zhi Ming Huang Xiao Hua Ye Chang Yuan Hu Guang Rong Lu De Yi Lu Igor Longe Phemba | 2014 | Journal of Clinical Gastroenterology2014，，3： | 2 |
| 6 | Double-bullet radioimmunotargeting therapy in 31 patients with primary liver cancer显示文摘Doublebuletradioimmunotargetingtherapyin31patientswithprimarylivercancerWUYingDe1,ZHOUDeNan2,GANGYouQuan2,HUXiaoHua1,LIZ... | WU Ying De 1, ZHOU De Nan 2, GANG You Quan 2, HU Xiao Hua 1, LI Zhi Ge 1, SONG Xiang Qun 1, HE Hai Ping 2, YANG Ke Zheng 1 and HUANG Bing Yan 1 1Department of Chemotherapy, Affiliated Cancer Hospital, Guangxi Medical University, | 1997 | World Journal of Gastroenterology1997，3，3： | 2 |
| 7 | Predicting densities and elastic moduli of SiO_(2)-based glasses by machine learning显示文摘Chemical design of SiO_(2)-based glasses with high elastic moduli and low weight is of great interest.However,it is difficult to find a universal expression to predict the elastic moduli according to the glass composition before synthesis since the elastic moduli are a complex function of interatomic bonds and their ordering at different length scales.Here we show that the densities and elastic moduli of SiO_(2)-based glasses can be efficiently predicted by machine learning(ML)techniques across a complex compositional space with multiple(>10)types of additive oxides besides SiO_(2).Our machine learning approach relies on a training set generated by high-throughput molecular dynamic(MD)simulations,a set of elaborately constructed descriptors that bridges the empirical statistical modeling with the fundamental physics of interatomic bonding,and a statistical learning/predicting model developed by implementing least absolute shrinkage and selection operator with a gradient boost machine(GBM-LASSO). | Yong-Jie Hu Ge Zhao Mingfei Zhang Bin Bin Tyler Del Rose Qian Zhao Qun Zu Yang Chen Xuekun Sun Maarten de Jong Liang Qi | 2020 | npj Computational Materials2020，，1： | 2 |
| 8 | Assessment of soil degradation and chemical compositions in Rwandan tea-growing areas显示文摘This study has focused on the processes of soil degradation and chemical element concentration in tea-growing regions of Rwanda,Africa.Soil degradation accelerated by erosion is caused not only by topography but also by human activities.This soil degradation involves both the physical loss and reduction in the amount of topsoil associated with nutrient decline.Soil samples were collected from eleven tropical zones in Rwanda and from variable depth within each collecting site.Of these,Samples from three locations in each zone were analyzed in the laboratory,with the result that the pH of all soil samples is shown to be less than 5(pH<5) with a general average of 4.4.The elements such as iron(Fe), copper(Cu),manganese(Mn),and zinc(Zn) are present in high concentration levels.In contrast calcium (Ca) and sodium(Na) are present at low-level concentrations and carbon(C) was found in minimal concentrations.In addition,elements derived from fertilizers,such as nitrogen(N),phosphorous(P),and potassium(K) which is also from minerals such as feldspar,are also present in low-level concentrations. The results indicate that the soil in certain Rwandan tea plantations is acidic and that this level of pH may help explain,in addition to natural factors,the deficiency of some elements such as Ca,Mg,P and N.The use of chemical fertilizers,land use system and the location of fields relative to household plots are also considered to help explain why tea plantation soils are typically degraded. | Jean de la Paix Mupenzi Lanhai Li Jiwen Ge Achal Varenyam Gabriel Habiyaremye Nzayisenga Theoneste Kamanzi Emmanuel | 2011 | Geoscience Frontiers2011，2，4： | 2 |
| 9 | Leaming representations by back-propagating errors显示文摘 | Rumelhart DE Hinton GE Williams RJ | 1986 | Nature1986，323，： | 1 |
| 10 | Comduction slowing and sudden arrhythmic death in mice with cardiac - restricted Inactiva- tion of connexin 43 显示文摘 | Gutstein DE Morley GE Tamaddon H | 2001 | Circ Res2001，88，3： | 1 |
| 11 | Hepatocarcinogenicity of chlordane in b6c3f1 and b6d2f1 male mice:evidence for regression in b6c3f1 mice and carcinogenesis independent of ras proto-oncogene activation显示文摘 | Malarkey DE Devereux TR Dinse GE | 1995 | Carcinogenesis1995，16，11： | 1 |
| 12 | Development of fluorine-substituted NH_(2)-biphenyl-diarylpyrimidines as highly potent non-nucleoside reverse transcriptase inhibitors:Boosting the safety and metabolic stability显示文摘Our recent studies for nonnucleoside reverse transcriptase inhibitors identified a highly potent compound JK-4b against WT HIV-1(EC_(50)=1.0 nmol/L),but the poor metabolic stability in human liver microsomes(t_(1/2)=14.6 min)and insufficient selectivity(SI=2059)with high cytotoxicity(CC_(50)=2.08μmol/L)remained major issues associated with JK-4b.The present efforts were devoted to the introduction of fluorine into the biphenyl ring of JK-4b,leading to the discovery of a novel series of fluorine-substituted NH_(2)-biphenyl-diarylpyrimidines with noticeable inhibitory activity toward WT HIV-1 strain(EC_(50)=1.8–349 nmol/L).The best compound 5t in this collection(EC_(50)=1.8 nmol/L,CC_(50)=117μmol/L)was 32-fold in selectivity(SI=66,443)compared to JK-4b and showed remarkable potency toward clinically multiple mutant strains,such as L100I,K103N,E138K,and Y181C.The metabolic stability of 5t was also significantly improved(t_(1/2)=74.52 min),approximately 5-fold higher than JK-4b in human liver microsomes(t_(1/2)=14.6 min).Also,5t possessed good stability in both human and monkey plasma.No significant in vitro inhibition effect toward CYP enzyme and hERG was observed.The single-dose acute toxicity test did not induce mice death or obvious pathological damage.These findings pave the way for further development of 5t as a drug candidate. | Xin Jin Shuai Wang Limin Zhao Wenjuan Huang Yinxiang Zhang Christophe Pannecouque Erik De Clercq Ge Meng Huri Piao Fener Chen | 2023 | Acta Pharmaceutica Sinica B2023，13，3： | 1 |
| 13 | Conduction slowing and sudden arrhythmic death in mice with cardiac-restricted inactivation of connexin43显示文摘 | Gutstein DE Morley GE Tamaddon H | 2001 | Circ Res2001，88，： | 1 |
| 14 | The cerebellar-hypothalamic axis: basic circuits and clinical observations 显示文摘 | Haines DE Dietrichs E Mihailoff GE | 1997 | Int Rev Neurobiol1997，41，： | 1 |
| 15 | Conduction slowing and sudden arrhythmic death in mice with cardiac-restricted inactivation of connexin 43 显示文摘 | Gutstein DE Morley GE Tamaddon H | 2001 | Circ Res2001，88，3： | 1 |
| 16 | SAPHO syndrome associated with hidradenitis suppurativa successfully treated with infliximab and methotrexate 显示文摘 | De Souza A Solomon GE Strober BE | 2011 | Bull NYU Hosp Jt Dis2011，69，2： | 1 |
| 17 | Physics-driven CFD modeling of complex anatomical cardiovascular flows-a TCPC case study显示文摘 | de Zélicourt D Ge L | 2005 | Ann Biomed Eng2005，33，3： | 1 |
| 18 | Conduction slowing and sudden arrhythmic death in mice with cardiac-restricted inactivation of connexin43显示文摘 | Gutstein DE Morley GE Tamaddon H | 2001 | Circ Res2001，88，： | 1 |
| 19 | Conduction slowing and sudden arrhythmic death in mice with cardiac restricted in activation of connexind-3显示文摘 | Gutstein DE Morley GE Tamaddon H | 2001 | Circ Res2001，88，3： | 1 |
| 20 | Modulation of paraoxonase 1 and 3 expression after moderate exercise training in the rat 显示文摘 | Romani R De Medio GE Di Tullio S | 2009 | J Lipid Res2009，50，10： | 1 |
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