|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Evaluation of acoustic radiation force impulse imaging for determination of liver stiffness using transient elastography as a reference显示文摘AIM: To evaluate cut-off values and performance of acoustic radiation force impulse imaging (ARFI) using transient elastography [FibroScan■ (FS)] as a reference. METHODS: Six hundred and six patients were enrolled in this study.All patients underwent liver stiffness measurement with FS (FS-LS) and ARFI (with shear wave velocity quantification; ARFI-SWV) and the performance of ARFI in comparison to FS was determined. Sixtyeight patients underwent liver biopsy. RESULTS: Significantly higher success rates for the determination of liver stiffness were found using ARFI as compared to FS [604/606 (99.7%) vs 482/606 (79.5%); P < 0.001]. ARFI-SWV correlated significantly with FS-LS (r = 0.920, P < 0.001). ARFI-SWV increased significantly with the stage of fibrosis (1.09 ± 0.13 m/s for patients with no significant fibrosis (FS-LS < 7.6 kPa); 1.46 ± 0.27 m/s for patients with significant liver fibrosis (7.6 < FS-LS ≤ 13.0 kPa); and 2.55 ± 0.77 m/s for patients with liver cirrhosis (FS-LS > 13.0 kPa)). ARFI-SWV cut-off values were identified for no significant fibrosis (1.29 m/s; sensitivity 91.4% and specificity 92.6%) and for liver cirrhosis (1.60 m/s; sensitivity 92.3% and specificity 96.5%). The optimal cut-off value for predicting liver fibrosis (F ≥ 2) was 1.32 m/s (sensitivity 87.0% and specificity 80.0%) and for liver cirrhosis (F4) 1.62 m/s (sensitivity 100% and specificity 85.7%), for patients who underwent liver biopsy. An excellent inter-and intraobserver reproducibility was observed for ARFI-SWV determinations. CONCLUSION: An ARFI-SWV cut-off value of 1.29 m/s seems to be optimal for patients with no significant liver fibrosis and 1.60 m/s for patients with liver cirrhosis. | Gerald Kircheis Abdurrahman Sagir Christoph Vogt Stephan vom Dahl Ralf Kubitz Dieter Hussinger | 2012 | World Journal of Gastroenterology2012，18，10： | 30 |
| 2 | New multi protein patterns differentiate liver fibrosis stages and hepatocellular carcinoma in chronic hepatitis C serum samples显示文摘瞄准:识别一多象用为肝纤维变性(F1-F2 ) 的察觉和区别的提高表面的激光 desorption/ionisation time-of-flight 团 spectrometry (SELDI-TOF-MS ) 的单个蛋白质标记一样的浆液蛋白质模式,在有长期的丙肝病毒(HCV ) 的病人的肝肝硬化(F4 ) andhepatocellular 癌(HCC ) 。方法:有 F1/F2 纤维变性的 39 个病人,的 Serumsamples 有 F4 纤维变性的 44 个病人,有我们重新申请到 CM10 数组并且分析了使用 SELDI-TOF ProteinChip 系统的 HCC 的 34 个病人(PBS-IIc;CiphergenBiosystems ) 在阴离子交换分别以后。所有病人 andhistologically 有长期的丙肝证实的纤维变性 stage/HCC。数据被 multivariatestatistical 技术和人工的神经网络为蛋白质模式分析。结果:4 肽 / 蛋白质 multimarkerpanel (7486, 12843, 44293 和 53598 Da ) 正确地在训练样品(AUROC0.943 ) 的 HCV 肝硬化对 HCV-HCC 的二方法比较与 100% 的敏感和 85% 的特性识别了 HCC。为 HCC 的鉴定的敏感和特性为随机的测试样品是 68% 和 80% 。肝脏硬化症的病人能被歧视有多使用 5peptide/protein 的 F1 或 F2 纤维变性的病人标记模式( 2873 , 6646 , 7775 , 10525 和 67867 Da )与100%和在训练样品( AUROC 0.976 )的85%的敏感和敏感和特性of80%的特性并且67%为随机的测试样品。有 APRI 分数 andalfa-fetopotein (法新社) 的简历标记分类器的联合改进了诊断表演。为肝纤维变性的 6646 Da 标记蛋白质作为 apolipoprotein C-I 被识别。结论:与蛋白质模式分析相结合的 SELDI-TOF-MS 技术与长期的丙肝在病人为肝肝硬化 andhepatocellular 癌的鉴定似乎是一条珍贵途径。最可能,不同浆液标记的联合将帮助以后识别肝肝硬化和早阶段的 hepatocellularcarcinomas。 | Thomas Gbel Sonja Vorderwülbecke Katarzyna Hauck Holger Fey Dieter Hussinger Andreas Erhardt | 2006 | World Journal of Gastroenterology2006，12，47： | 21 |
| 3 | Metastasized pancreatic carcinoma with neoadjuvant FOLFIRINOX therapy and R0 resection显示文摘Patients with metastasized carcinoma of the pancreas have a very poor prognosis, and long-term survival cannot be expected. This case report describes two patients with an initial diagnosis of metastatic pancreatic cancer, both with hepatic metastases and one with an additional peritoneal carcinomatosis. Initially, both patients were treated intravenously with the FOLFIRINOX chemotherapy regimen, consisting of 5-FU, folinic acid, irinotecan and oxaliplatin. Surprisingly, the FOLFIRINOX treatment resulted in complete resolution of the hepatic metastases in both patients, with no lesions detectable by computed tomography scan. Furthermore, treatment response included decreased diameter of the primary tumor in the tail of the pancreas and disappearance of the additional peritoneal carcinomatosis. Both patients were discussed by our multidisciplinary tumor board, which recommended surgical resections of the carcinoma. The R0 resection of the primary tumor was successful in both cases and, interestingly, the resected tissues showed no evidence of the hepatic metastases intraoperatively. In the first case, the patient received a postoperative 6-mo course of adjuvant chemotherapy with gemcitabine. In the second case, the patient continued to receive the FOLFIRINOX regimen for an additional 6 mo postoperatively. At 12 mo after the operation, a nonresectable retroperitoneal lymph node metastasis was detected in the first patient, whereas the second patient remained in complete remission at the time of this report(5 mo after the adjuvant therapy was discontinued). This case report is the first of its kind to describe two cases of hepatic metastatic pancreatic carcinoma that were resectable following treatment with FOLFIRINOX. Further studies are required to examine the role of FOLFIRINOX as a neoadjuvant treatment option in subgroups of patients with initially metastasized pancreatic carcinoma. | Sophie Schneitler Patric Krpil Jasmin Riemer Gerald Antoch Wolfram Trudo Knoefel Dieter Hussinger Dirk Graf | 2015 | World Journal of Gastroenterology2015，21，20： | 9 |
| 4 | Endocrine and paracrine role of bile acids显示文摘Bile acids are not only important for the absorption of dietary lipids and fat soluble vitamins but are signalling molecules with diverse endocrine and paracrine functions. Bile acids regulate bile acid, lipid and glucose metabolism and modulate temperature and energy homeostasis. Furthermore, bile acids can not only promote cell proliferation and liver regeneration but can also induce programmed cell death. Bile acid functions are mediated through different pathways which comprise the activation of nuclear hormone receptors, of intracellular kinases and of the plasma membrane-bound, G-protein coupled bile acid receptor TGR5/Gp-bar-1. | Verena Keitel Ralf Kubitz Dieter Hussinger | 2008 | World Journal of Gastroenterology2008，14，37： | 7 |
| 5 | FibroSURE^(TM) and FibroScan~ in relation to treatment response in chronic hepatitis C virus显示文摘AIM:To compare histological endpoint assessment using noninvasive alternatives to biopsy during treatment in a chronic hepatitis C virus(HCV)cohort.METHODS:Patients with chronic HCV were randomized to receive interferon-based therapy for 24(genotypes 2/3)or 48(genotype 1)wk.FibroSURE~TM(FS)was assessed at baseline and at week-12 post-treatment follow-up.Baseline biopsy for METAVIR was assessed by a single pathologist.FibroScan~ transient elastogra-phy(TE)was performed during treatment in a patient subset.RESULTS:Two thousand and sixty patients(n = 253 in Asia)were classif ied as METAVIR F0-1(n = 1682)or F2-4(n = 378).For F2-4,FS(n = 2055)had sensitiv-ity and specif icity of 0.87 and 0.61,respectively,with area under the receiver-operating curve of 0.82;corre-sponding values for TE(n = 214)and combined FS/TE(n = 209)were 0.77,0.88 and 0.88,and 0.93,0.68 and 0.88.Overall FS/TE agreement for F2-4 was 71%(κ = 0.41)and higher in Asians vs non-Asians(κ = 0.86 vs 0.35;P < 0.001).Combined FS/TE had 97% accuracy in Asians(n = 33).Baseline FS(0.38 vs 0.51,P < 0.001)and TE(8.0 kPa vs 11.9 kPa,P = 0.006)scores were lower in patients with sustained virological response than in nonresponders,and were maintained through follow-up.CONCLUSION:FS and TE may reliably differentiate mild from moderate-advanced disease,with a potential for high diagnostic accuracy in Asians with chronic HCV. | Keyur Patel Mireen Friedrich-Rust Yoav Lurie Mircea Grigorescu Carol Stanciu Chuan-Mo Lee Eugene R Schiff Dieter Hussinger Michael P Manns Guido Gerken Isabelle Colle Michael Torbenson Erik Pulkstenis G Mani Subramanian John G McHutchison Stefan Zeuzem | 2011 | World Journal of Gastroenterology2011，17，41： | 4 |
| 6 | Neoadjuvant peptide receptor radionuclide therapy for an inoperable neuroendocrine pancreatic tumor显示文摘Pancreatic endocrine tumors are rare but are among the most common neuroendocrine neoplasms of the abdomen.At diagnosis many of them are already advanced and diff icult to treat.We report on an initially inoperable malignant pancreatic endocrine tumor in a 33-year-old woman,who received neoadjuvant peptide receptor radionuclide therapy(PRRT)as firstline treatment.This resulted in a signif icant downstaging of the tumor and allowed its subsequent complete surgical removal.Follow-up for eighteen months revealed a complete remission.This is the first report on neoadjuvant PRRT in a neuroendocrine neoplasm with subsequent successful complete resection. | Daniel Kaemmerer Vikas Prasad Wolfgang Daffner Dieter Hrsch Günter Klppel Merten Hommann Richard P Baum | 2009 | World Journal of Gastroenterology2009，15，46： | 2 |
| 7 | SEN virus does not affect treatment response in hepatitis C virus coinfected patients but SEN virus response depends on SEN virus DNA concentration显示文摘AIM: To clarify the effect of SEN virus (SENV) infection on a combination therapy including interferon alfa (IFN-α) or pegylated-IFN with ribavirin in patients with chronic hepatitis and the effect of a combination therapy on SENV.METHODS: SENV DNA was determined by polymerase chain reaction in serum samples from 95 patients with chronic hepatitis C. Quantitative analysis was done for SENV H DNA.RESULTS: Twenty-one (22%) of 95 patients were positive for SENV DNA. There was no difference in clinical and biochemical parameters between patients with HCV infection alone and coinfected patients. The sustained response rate for HCV clearance after combination therapy did not differ between patients with SENV (52%) and without SENV(50%, n.s.). SENV DNA was undetectable in 76% of the initially SENV positive patients at the end of follow-up. SENV H response to combination therapy was significantly correlated with SENV DNA level (P=-0.05).CONCLUSION: SENV infection had no influence on the HCV sustained response rate to the combination therapy.Response rate of SENV to the combination therapy depends on SENV DNA level. | Abdurrahman Sagin Ortwin Adams OliverKirschberg AndreasErhardt TobiasHeintges Dieter Hussinger | 2004 | World Journal of Gastroenterology2004，10，13： | 2 |
| 8 | Genotype-dependent activation or repression of HBV enhancer Ⅱ by transcription factor COUP-TF1显示文摘AIM: To study the expression of HBV enhancer Ⅱ by transcription factor COUP-TF1. METHODS: In order to study the regulation of HBV variants in the vicinity of the NRRE we cloned luciferase constructs containing the HBV enhancer Ⅱ from variants and from HBV genotypes A and D and cotransfected them together with expression vectors for COUP-TF1 into HepG2 cells. RESULTS: Our fi ndings show that enhancer Ⅱ of HBV genotype A is also repressed by COUP-TF1. In contrast, two different enhancer Ⅱ constructs of HBV genotype D were activated by COUP-TF1. The activation was independent of the NRRE because a natural variant with a deletion of nt 1763-1770 was still activated by COUP- TF1. CONCLUSION: Regulation of transcription of the HBV genome seems to differ among HBV genomes derived from different genotypes. These differences in transcriptional control among HBV genotypes may be the molecular basis for differences in the clinical course among HBV genotypes. | Silke F Fischer Katja Schmidt Nicola Fiedler Dieter Glebe Christian Schüttler Jianguang Sun Wolfram H Gerlich Reinald Repp Stephan Schaefer | 2006 | World Journal of Gastroenterology2006，12，37： | 2 |
| 9 | Osmotic and oxidative/nitrosative stress in ammonia toxicity and hepatic encephalopathy显示文摘 | Boris G?rg Freimut Schliess Dieter H?ussinger | 2013 | Archives of Biochemistry and Biophysics2013，，2： | 2 |
| 10 | Trypsinogen activation and glutathione content are linked to pancreatic injury in models of biliary acute pancreatitis显示文摘 | Reinhard Lüthen James H. Grendell Claus Niederau Dieter H?ussinger | 1998 | International Journal of Pancreatology1998，，3： | 2 |
| 11 | SPQD14120800011178显示文摘 | Kordes Claus Sawitza Iris G?tze Silke Herebian Diran H?ussinger Dieter | 2014 | Journal of Clinical Investigation2014，，12： | 2 |
| 12 | Predominant mucosal expression of 5-HT_(4(+h)) receptor splice variants in pig stomach and colon显示文摘AIM: To investigate cellular 5-HT4(-h/+h) receptor distribution, particularly in the epithelial layer, by laser mi-crodissection and polymerase chain reaction (PCR) in porcine gastrointestinal (GI) tissues. METHODS: A stepwise approach was used to evaluate RNA quality and to study cell-specific 5-HT4 receptor mRNA expression in the porcine gastric fundus and colon descendens. After freezing, staining and laser microdissection and pressure catapulting (LMPC), RNA quality was evaluated by the Experion automated electrophoresis system. 5-HT4 receptor and glyceral-dehyde-3-phosphate dehydrogenase (GAPDH) expressions were examined by endpoint reverse transcription (RT)-PCR in mucosal and muscle-myenteric plexus (MMP) tissue fractions, in mucosal and MMP parts of hematoxylin and eosin (HE) stained tissue sections andin microdissected patches of the epithelial and circular smooth muscle cell layer in these sections. Pig gastric fundus tissue sections were also stained immunohisto-chemically (IHC) for enterochromaffin cells (EC cells; MAB352); these cells were isolated by LMPC and examined by endpoint RT-PCR. RESULTS: After HE staining, the epithelial and circular smooth muscle cell layer of pig colon descendens and the epithelial cell layer of gastric fundus were identified morphologically and isolated by LMPC. EC cells of pig gastric fundus were successfully stained by IHC and isolated by LMPC. Freezing, HE and IHC staining, and LMPC had no influence on RNA quality. 5-HT4 recep-tor and GAPDH mRNA expressions were detected in mucosa and MMP tissue fractions, and in mucosal and MMP parts of HE stained tissue sections of pig colon descendens and gastric fundus. In the mucosa tissue fractions of both GI regions, the expression of h-exon containing receptor [5-HT4(+h) receptor] mRNA was significantly higher (P<0.01) compared to 5-HT4(-h) re-ceptor expression, and a similar trend was obtained in the mucosal part of HE stained tissue sections. Large microdissected patches of the epithelial and circular smooth muscle cell layer of pig colon descendens and of the epithelial cell layer of pig gastric fundus, also showed 5-HT4 receptor and GAPDH mRNA expression. No 5-HT4 receptor mRNA expression was detected in gastric LMPC-isolated EC cells from IHC stained tissues, which cells were positive for GAPDH. CONCLUSION: Porcine GI mucosa predominantly expresses 5-HT4(+h) receptor splice variants, suggesting their contribution to the 5-HT4 receptor-mediated mu-cosal effects of 5-HT. | Evelien KV Priem Joris H De Maeyer Mado Vandewoestyne Dieter Deforce Romain A Lefebvre | 2013 | World Journal of Gastroenterology2013，19，24： | 2 |
| 13 | Control strategy for NOx-emission reduction with SCR 显示文摘 | Dieter H E Seher Michael Reichelt Stefan Wickert | 2003 | SAE Paper2003，，1： | 1 |
| 14 | Calculation of the nonlinear optical properties of molecules 显示文摘 | Kurtz H A Stewart J J P Dieter K M | 1990 | Journal of Computa- tional Chemistry1990，11，1： | 1 |
| 15 | Osmoregulation of Bile Formation显示文摘 | Ralf Kubitz Dieter H?ussinger | 2007 | Methods in Enzymology2007，，： | 1 |
| 16 | Low seroprevalence of Helico- bacter pylori infection in patients with stress ulcer bleeding--a pro- spective evaluation of patients on a cardiosurgical intensive care unit显示文摘 | Dieter S Cunter H Norbert S | 2000 | Intensive Care Med2000，26，12： | 1 |
| 17 | Water, K+, H+, lactate and glucose fluxes during cell volume regulation in perfused rat liver显示文摘 | Florian Lang Thomas Stehle Dieter H?ussinger | 1989 | Pflügers Archiv European Journal of Physiology1989，，3： | 1 |
| 18 | Quality assessment insurgery 显示文摘 | Daniel D Dieter H Clavien PA | 2010 | Ann Surg2010，251，4： | 1 |
| 19 | Oral exposure to inorganic arsenic: evaluation of its carcinogenic and non-carcinogenic effects 显示文摘 | Schuhmacher-Wolz U Dieter H H Klein D | 2009 | Crit RevToxicol2009，39，4： | 1 |
| 20 | Schlag,Evolutionarily stable sets显示文摘 | Dieter Balkenborg Karl H | 2001 | International Journal Game Theory2001，，29： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员