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| 1 | Cloning of first abc transporter encoding gene from Trichoderma spp. and its expression during stress and mycoparasitism显示文摘Trichoderma in its natural environment competes for nutrient uptake and is required to protect itself from adverse natural toxic compounds, such as those produced by plants and other microbes in the soil community, or synthetic toxic compounds released human activity. One of the most important metabolic pathways for drug resistance and substrate uptake, both in prokaryotes and eukaryotes, is ATP dependent. The role of ABC transporter proteins in the biology of Trichoderma is still not known. We present the cloning of the first four ABC transporter genes (TABC1, TABC2, TABC3, TABC4 ) in Trichoderma, and in particular T. atroviride P1, and the characterization of TABC2 The complete sequence of this gene is 6535 bp, which includes a promoter of 1624 bp, a terminator of 642 bp and a coding region of 4264 bp. The promoter contains many of the potential transcription factor binding sites found in the 5’ upstream region of the ech42 gene of T. atroviride P1. These included: heat shock factors (HSF), a nitrogen-regulating factor (Nit-2), a stress-response element (STRE), a GCR1 elements, and a Cre BP1 motif. Northern analysis and RT-PCR demonstrated that TABC2 is highly expressed when Trichoderma is subjected to nitrogen starvation, grown in the presence of culture filtrates of Botrytis cinerea, Rhizoctonia solani, and Pythium ultimum, or when N-acetylglucosamine is added to the substrate. TABC2 appears to be co-regulated with some CWDE-encoding genes, suggesting that this is the first ABC transporter encoding gene involved in mycoparasitic events. It’s role in the interaction of Trichoderma with fungal hosts or plants is being investigated by targeted gene disruption and overexpression. | Lanzuise S Ruocco M Scala V Catapano L Woo S Ciliento R Ferraioli S Soriente I Vinale F Scala F Del Sorbo G Lorito M | 2004 | 浙江大学学报(农业与生命科学版)2004，30，4： | 2 |
| 2 | Localization of manganese-superoxide dismutase in peroxisomes isolated from Pisum sativum L显示文摘 | Sandalio L M Palma J M del Río L A | 1987 | Plant Science1987，51，： | 1 |
| 3 | Helicobacter pylori infection and gastric MALTomas: an up-to- date and therapy highlight 显示文摘 | SANTACROCE L CAGIANO R DEL PRETE R | 2008 | Clin Ther2008，159，6： | 1 |
| 4 | On-line preconcentration and speciation of arsenic by flow injection hydride generation atomic absorption spectrophotometry显示文摘 | NARCISE C I S COO L C DEL MUNDO F R | 2005 | Talanta2005，68，： | 1 |
| 5 | Improving the Cercospora leaf spot management model for sugar beet in Minnesota and North Dakota 显示文摘 | Khan J Del Rio L E Nelson R | 2007 | Plant Disease2007，91，9： | 1 |
| 6 | Rosiglitazone prevents theimpairment of human islet function induced by fatty acids: evidencefor a role of PPAR-g2 in the modulation of insulin secretion 显示文摘 | Lupi R Del Guerra S Marselli L | 2004 | Am J Physiol Endocrinol Metab2004，286，4： | 1 |
| 7 | Nonconscious semantic processing of emotional words modulates conscious access显示文摘 | Gaillard R Del Cul A Naccache L | | 0，，： | 1 |
| 8 | Artiodactyl emergence is accompanied by the birth of an extensive pool of diverse germline TRDVl genes显示文摘 | Antonacci R Lanave C Del Faro L | 2005 | Immunogenetics2005，57，： | 1 |
| 9 | Transient hypoxia ischemia in rats: changes in diffusion sensitive MR imaging findings, extracellular space, and Na + -K + -adenosine triphosphatasc and cytochrome oxidase activity 显示文摘 | Qiao M Malisza K L Del Bigio M R | 2002 | Radiology2002，223，： | 1 |
| 10 | Macrophage migration inhibitory factor in prostatic adenocarcinoma:correlation with tumor grading and combination endocrine treatment-related changes显示文摘 | del Vecchio MT Tripodi SA Arcuri F Pergola L Hako L Vatti R | 2000 | Prostate2000，45，1： | 1 |
| 11 | Systemic lack of ca- nonical histamine receptor signaling results in increased resistance to autoimmune encephalomyelitis 显示文摘 | SALIGRAMA N CASE L K DEL RIO R | 2013 | J Immunol2013，191，2： | 1 |
| 12 | The diagnostic value of CD123 in B-cell disorders with hairy or villous lymphocytes 显示文摘 | Del Giudice L Matutes E Monlla R | 2004 | Haemat Ologiea2004，89，3： | 1 |
| 13 | The use of unenhanced Doppler sonography in the evaluation of solid breast lesions显示文摘 | DEL CURA J L ELIZAGARAY E ZABALA R etal | 2005 | AJR Am J Roentgenol2005，184，6： | 1 |
| 14 | Next-generation Mobile Satellite Networks显示文摘 | Del R E Pierucci L | 2002 | IEEE Communication Magazine2002，40，9： | 1 |
| 15 | Conductivity Studies of Solid Polymer Electrolytes Based on Polyethers and Polyphosphazene Blends显示文摘 | Martin A P J Acosta J L | 1998 | Journal of Applied Polymer Science1998，70，11： | 1 |
| 16 | Study of the enantiomeric composition of chiral constituents in edible oils by simultaneous distillation-extraction, detection of adulterated olive oils 显示文摘 | CAJA M M RUIZ del CASTILLO M L R HERRAIZ M | 1999 | Journal of the American Oil Chemists'' Society1999，76，9： | 1 |
| 17 | Endothelial adhesion molecules ICAM-1,VCAM-1 and E-selectin in patients with acute coronary syndrome显示文摘 | Macias C Villaescusa R del Valle L | 2003 | Rev ESP Cardiol2003，56，： | 1 |
| 18 | Seabed morphology and hydrocarbon seep age in the Gulf of Cadiz mud volcano area:Acoustic imagery,multibeam and ultra-high resolution seismic data显示文摘 | Somoza L Diaz del Rio V Len R | | 0，，14： | 1 |
| 19 | MRI role in morphological and functional assessment of the levator ani muscle: use in patients affected by stress urinary incontinence ( SUI ) before and after pelvic floor rehabilitation显示文摘 | Del Vescovo R Piccolo C L Della Vecchia N | 2014 | European Journal of Radiology2014，83，3： | 1 |
| 20 | Potential of particle and fibre reinforcement of tyre tread elastomers显示文摘 | RJOS S CHICUREL R DEL CASTILLO L F | 2001 | Materials and Dcsign2001，22，： | 1 |
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