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| 1 | Comparison of Wirsung-jejunal duct-tomucosa and dunking technique for pancreatojejunostomy after pancreatoduodenectomy显示文摘Pancreato-enteric reconstruction after pancreatoduodenectomy (PD) is still a source of debate because of the high incidence of complications. Among the various types of pancreato-jejunostomies we don’t know yet which is the best in terms of anastomotic failure and related complications rates. Wirsung-jejunal duct-to-mucosa anastomosis (WJ) and 'dunking' pancreato-jejunal anastomosis (DPJ) are the two most used ones worldwide but conflicting results are reported. To determine which is the safer anastomosis and to define when an anastomosis should be preferred, we retrospectively reviewed two groups of patients who underwent WJ or DPJ. METHODS:Twenty-three patients underwent PD with WJ (n=17) with dilated (WJD) (n=9) or not-dilated Wirsung’s duct (WJND) (n=8) or with a DPJ (n=6) over a 3-year period at a single institution. RESULTS: The complications rate was high in all groups of patients (33.3% in WJD, 37.5% in WJND and 66.7% in DPJ). A pancreatic fistula developed in one patient in each group (11. 1% in WJD, 12. 5% in WJND and 16. 7% in DPJ). All these patients were managed conservatively. Anastomotic disruption took place in the WJ patients especially in the WJND group (n=2) compared to the WJD (n=1) (25% vs 11.1%) or DPJ groups (0%) : these three patients required a re-operation. Overall, the anastomotic defects were higher in patients who underwent WJND (37.5%), compared to WJD (22.2%) and to DPJ (16.7%). However, no statistical differences were found among the groups. Delayed gastric emptying (DGE) and total parenteral nutrition (TPN) along with anastomotic defects were responsible for a prolonged hospital stay. CONCLUSIONS:Our results were not able to demonstrate any statistical difference between the two different techniques in preventing anastomotic failure. WJ can represent a valid choice in case of a dilated duct and a firm, fibrotic enlarged gland that could not be properly invaginated in a small jejunal loop. DGE may occur in those patients who experienced an anastomotic failure and required a TPN regimen with a prolonged hospital stay. | Unita di Chirurgia, Dipartimento di Fisiopatologia Clinica (Batignani G, Fratini G, Zuckermann M and Tonelli F) and Dipartimento di Statistica (Bianchini E), Universitd degli Studi di Firenze, Flo rence, Italy | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 9 |
| 2 | Identification and characterization of Trichoderma species aggressive to Pleurotus in Italy显示文摘In the late 1980’s the deve lop ment of a severe epidemic of green mold caused by Trichoderma spp. was not ed in the commercial production of Agaricus bisporus (champignon) in the U nited Kingdom, North America, Spain and Holland, which caused extensive economic losses. The parasitic fungi isolated from the edible mushroom belonged to four biotypes, Th1, Th2, Th3 and Th4 of T. harzianum. However, among these biotypes, only Th2 (since c lassified as T. aggressivum f. europaeum) and Th4 (T. aggressivum f. aggressivum) were identified as the fungi causing problems in Agaricus production. In general, mushroom compost hosts both aggressive and innocuous is olates of Trichoderma, which are not morphologically distinguishable. Abo ut four years ago, a problem with green mold became apparent in the production o f Pleurotus ostreatus in Northern Italy, which eventually developed to a c risis situation in the South two years later and threatened to seriously comprom ise the Pleurotus market. This study was initiated to: isolate and identif y the aggressive fungi, then morphologically, physiologically and genetically characterize the isolates, dete rmine the source and phases of infection, and study methods of control. Samples were obtained from different phases of compost preparation at the locality of a major producer and supplier of compost to the mushroom industry in Southern Ital y, and microbial counts were conducted. Although the presence of Trichoderma was detected in the initial stages of composting, this value was reduced to zero from the phase of pasteurization to seeding with Pleurotus. Trichoderma infestations were noted in the packaged Pleurotus bales at various time s during the incubation phase (7-15 days after seeding) and after shipping to th e mushroom greenhouses, where the pathogen infestations greatly reduced the qual ity and quantity of the mushroom yield, as well as the number of potential harvest cycles. Preliminary r esults from the morphological and genetic characterization of Trichoderma isolates parasitic to Pleurotus indicated that they are different from bot h T. aggressivum forms parasitic to Agaricus, and the majority of the isolates probably belong to the species T. harzianum. In vitr o confrontation plates were performed with 26 isolates of aggressive Trich oderma obtained from compost, three Trichoderma isolates used in biolog ical control and 12 varieties of Pleurotus. No inhibitory effect was obse rved between any of the Trichoderma isolates with Pleurotus, althou gh some growth inhibition was caused by the biocontrol isolates of Trichoderm a on some of the aggressive isolates. The temperature optimum for Pleurotus growth was at 28 ℃, whereas Trichoderma grew well at a wider range (20- 28 ℃), and exceeded the growth rate of Pleurotus by three times at 25 ℃. T he pH optimum for the growth of Pleurotus was alkaline (pH 8-9) whereas Trichoderma preferred acidic-neutral pH (5-7) . Various commercial fungicides used in agriculture (procloraz, thiabendazole, dichloran, benomyl, p r opiconazole, thiofanatomethyl) were tested against the aggressive and biocontro l isolates of Trichoderma, as well as the different varieties of Pleuro tus to determine dose response curves and combinations that would inhibit spo re germination, mycelial growth and subsequent sporulation. Both procloraz and thiabendazole, which are pesticides allowed in e dible mushroom production, were found to control the growth of the aggressive Trichoderma isolates and did not have a negative effect on Pleurotus. | Woo S L Di Benedetto P Senatore M Abadi K Gigante S Soriente I Ferraioli S Scala F Lorito M | 2004 | 浙江大学学报(农业与生命科学版)2004，30，4： | 2 |
| 3 | 遗传性肥胖与心房颤动的关系显示文摘观察性研究已经确定了体质量指数(body mass index,BMI)与心房颤动之间的关联。然而,从观察性研究中推断因果关系会受到混杂因素、逆因果关系和偏倚的影响。该研究的主要目的是应用BMI的遗传学预测因子评估BMI与心房颤动之间的因果关系。方法:纳入1987-2002年期间在美国、冰岛和荷兰实施的7个前瞻性人群队列研究中基线无心房颤动的欧洲血统个体51 646人, | 刘莉 叶鹏 Chatterjee NA Giulianini F Geelhoed B Lunetta KL Misialek JR Niemeijer MN Rienstra M Rose LM Smith AV Arking DE Ellinor PT Heeringa J Lin H Lubitz SA Soliman EZ Verweij N Alonso A Benjamin EJ Gudnason V Stricker BH van der Harst P Chasman DI Albert CM | 2017 | 中华高血压杂志2017，25，2： | 2 |
| 4 | Genetic improvement of Trichoderma ability to induce systemic resistance显示文摘The beneficial applications of Trichoderma spp. in agriculture include not only the control of plant pathogens, but also the improvement of plant growth, micronutrient availability, and plant tolerance to abiotic stress. In addition, it has been suggested that these fungi are able to increase plant disease resistance by activating induced systemic resistance (ISR) . The mode of action of these beneficial fungi in the Trichoderma -plant-pathogen interaction are many, complex and not completely understood. Numerous lytic enzymes have been characterized, the encoding genes (ech42 gluc78, nag1 from T. atroviride strain P1) cloned, and their role in biocontrol demonstrated. The corresponding biocontrol-related inducible promoters have been used in a reporter system based on the Aspergillus niger glucose oxidase gene (goxA) to monitor biocontrol activity. Glucose oxidase catalyzes the oxygen-dependent oxidation of D-glucose to D-glucono-1,5-lactone and hydrogen peroxide; this latter compound is known to have an antifungal effect and activate the plant defence cascade, thus increasing resistance to pathogen attack. T. atroviride P1 transformants with various promoters gox were tested as seed coating treatments on bean seeds planted in soil infested with a soilborne fungal pathogen. Successively, the emergent leaves were inoculated with a foliar pathogen to determine the effect of the GOX transformants on biocontrol and resistance to pathogen attack. Inoculations with the P1-GOX transformants not only reduced disease symptoms caused by a soil pathogen, but also the lesions of various foliar pathogens applied far from the Trichoderma colonization, thus activating ISR. A similar approach is being use to genetically improve T. harzianum T22, a rhizosphere competent and commercially marketed strain not transformed yet, by using four different gox gene constructs under the control of constitutive and inducible promoters. Plasmids have been introduced in Trichoderma by protoplasts co-transformation. hygromicin resistant progeny selected, and mitotically stable transformants analysed to confirm the presence of the novel enzyme activity. Progenies are being tested for biocontrol ISR inducing activity. | Ciliento R Woo S L Di Benedetto P Ruocco M Scala F Soriente I Ferraioli S Brunner K Zeilinger S Mach R L Lorito M | 2004 | 浙江大学学报(农业与生命科学版)2004，30，4： | 2 |
| 5 | Molecular mechanisms of acute renal failure following ischemia reperfusion显示文摘 | VRTDYRILRN A M DI - MAGGIO F LEEMREIS J R | 2004 | Int J Artif Organs2004，27，12： | 1 |
| 6 | Complications of thoracic pedicle screws in scoliosis treatment显示文摘 | Di Silvestre M Parisini P Lolli F | 2007 | Spine2007，32，： | 1 |
| 7 | Individual patient data meta-analysis of docetaxel administered once every 3 weeks compared with once every week second line treat- ment of advanced non-small-cell lung cancer 显示文摘 | Di Maio M Pernme F Chiodini P | 2007 | J Clin Oncol2007，25，11： | 1 |
| 8 | Iodide excess induces apoptosis in thyroid cells through a p53-independent mechanism involving oxidative stress显示文摘 | Vitale M Di Matola T D'Ascoli F | 2000 | Endocrinology2000，141，2： | 1 |
| 9 | Wildtype BRAF is required for response to panitumumab or eetuximah in metastatic colorectal cancer 显示文摘 | Di Nicolantonio F Martini M Molinarl F | 2008 | J Clin Oncol2008，26，35： | 1 |
| 10 | Wild-type BRAF is required for response to panitumumab or cetuximab in metastatic colorectal cancer显示文摘 | Di Nicolantonio F Martini M Molinari F | 2008 | J Clin Oneol2008，26，35： | 1 |
| 11 | Molecular characterization of an endopolygalacturonase from Fusarium oxysporum expressed during early stages of infection显示文摘 | Garcia-Maceira F I DI Pietro A Huertas-Gonztles M D | 2001 | Applied and Environmental Microbiology2001，67，： | 1 |
| 12 | TCR engagement regulates differential reponsiveness of human memory T cells to FasL (CD95)-mediated apoptosis 显示文摘 | Di Somma M M Somma F Montani M S G | 1999 | J Immunology1999，162，1： | 1 |
| 13 | Improved Gain Characteristics in High-Concentration Er^3+/Yb^3+ Codoped Glass Waveguide Amplifiers显示文摘 | Di Pasquale F and Federighi M | 1994 | IEEE J Quantum Electronics1994，30，9： | 1 |
| 14 | Wild-type BRAF is required for responseto panitumurnab or cetuximab in metastatic colorectal cancer显示文摘 | DI NICOLANTONIO F MARTINI M MOLINARI F | 2008 | J Clin Oncol2008，26，35： | 1 |
| 15 | Efficient visible laser emission of GaN laser diode pumped Pr-doped fluoride crystals显示文摘 | F Cornacchia A Di Lieto M Tonelli | 2008 | Opt Express2008，16，15： | 1 |
| 16 | Vibrant soundbridge in congenital osseous atresia: multicenter study of' 12 patients with osseous atrcsia 显示文摘 | Zemotti M E Arauz S L Di Gregorio M F | 2013 | Acta Otolaryngol2013，133，6： | 1 |
| 17 | Prevalence of sarcopenia and its association with osteoporosis in 313 older women following a hip fracture 显示文摘 | Di Monaco M Vallero F Di Monaco R | 2011 | Arch Gerontol Geriatr2011，52，： | 1 |
| 18 | Vulvovaginitis in childhood显示文摘 | Dei M Di Maggio F Di Paolo G | 2010 | Best Pract Res Clin Obstet Gynaecol2010，24，2： | 1 |
| 19 | Adult's degenerative scoliosis:midterm results of dynamic stabilization without fusion in elderly patients-is it effective显示文摘 | Di Silvestre M Lolli F Greggi T | 2013 | Adv Orthop2013，2013，36： | 1 |
| 20 | Role of tumor necrosis fac- tor alpha release and leukocyte margination in indomethacin-in-duced gastric injury in rats显示文摘 | Santucci L Fiorucci S Di M F M | 1995 | Gastroenterology1995，108，2： | 1 |
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