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| 1 | Combination of epidural electrical stimulation with ex vivo triple gene therapy for spinal cord injury:a proof of principle study显示文摘Despite emerging contemporary biotechnological methods such as gene-and stem cell-based therapy,there are no clinically established therapeutic strategies for neural regeneration after spinal cord injury.Our previous studies have demonstrated that transplantation of genetically engineered human umbilical cord blood mononuclear cells producing three recombinant therapeutic molecules,including vascular endothelial growth factor(VEGF),glial cell-line derived neurotrophic factor(GDNF),and neural cell adhesion molecule(NCAM)can improve morpho-functional recovery of injured spinal cord in rats and mini-pigs.To investigate the efficacy of human umbilical cord blood mononuclear cells-mediated triple-gene therapy combined with epidural electrical stimulation in the treatment of spinal cord injury,in this study,rats with moderate spinal cord contusion injury were intrathecally infused with human umbilical cord blood mononuclear cells expressing recombinant genes VEGF165,GDNF,NCAM1 at 4 hours after spinal cord injury.Three days after injury,epidural stimulations were given simultaneously above the lesion site at C5(to stimulate the cervical network related to forelimb functions)and below the lesion site at L2(to activate the central pattern generators)every other day for 4 weeks.Rats subjected to the combined treatment showed a limited functional improvement of the knee joint,high preservation of muscle fiber area in tibialis anterior muscle and increased H/M ratio in gastrocnemius muscle 30 days after spinal cord injury.However,beneficial cellular outcomes such as reduced apoptosis and increased sparing of the gray and white matters,and enhanced expression of heat shock and synaptic proteins were found in rats with spinal cord injury subjected to the combined epidural electrical stimulation with gene therapy.This study presents the first proof of principle study of combination of the multisite epidural electrical stimulation with ex vivo triple gene therapy(VEGF,GDNF and NCAM)for treatment of spinal cord injury in rat models.The animal protocols were approved by the Kazan State Medical University Animal Care and Use Committee(approval No.2.20.02.18)on February 20,2018. | Filip Olegovich Fadeev Farid Vagizovich Bashirov Vahe Arshaluysovich Markosyan Andrey Alexandrovich Izmailov Tatyana Vyacheslavovna Povysheva Mikhail Evgenyevich Sokolov Maxim Sergeevich Kuznetsov Anton Alexandrovich Eremeev Ilnur Ildusovich Salafutdinov Albert Anatolyevich Rizvanov Hyun Joon Lee Rustem Robertovich Islamov | 2021 | Neural Regeneration Research2021，16，3： | 4 |
| 2 | Optimization of enzymatic hydrolysis of animal raw material forobtaining functional meat protein preparation显示文摘 | NIKOLAEV I V STEPANOVA E V EREMEEV N L | 2008 | Biotechnology(Moscow)2008，5，： | 1 |
| 3 | 24-84-GHz Gyrotron Systems for Technological Microwave Applications显示文摘 | Bykov Y Eremeev A | 2004 | IEEE Transactions on Plasma Science2004，32，1： | 1 |
| 4 | Enzymatic hydrolysis of keratin containing stock for obtaining protein hydrolysates显示文摘 | Eremeev NL Nikolaev IV Keruchen' ko ID | 2009 | Applied Biochemistry and Microbiology2009，45，6： | 1 |
| 5 | Knzymatichydrolysis of keratin containing stock for obtaining proteinhydrolysates 显示文摘 | Eremeev NL Nikolaev IV Keruchen ' ko ID | 2009 | Appl ied Biochemistry and Microbiology2009，45，6： | 1 |
| 6 | Newclass of cryptographic primitives and cipher design fornetworks security 显示文摘 | Moldovyan N A Moldovyan A A Eremeev M A | 2006 | International Journal of NetworkSecurity2006，2，2： | 1 |
| 7 | A vacuum filter used to dewater sediment from industrial effluents 显示文摘 | Eremeev B B Yu G Pikulin M | 2003 | Chemical and Petroleum Engineering2003，39，1112： | 1 |
| 8 | Synthesis and properties of 4-amino-3-cyanofurazan显示文摘 | Andrianov V G Eremeev A V | 1994 | Che- mistry of Heterocyclic Compounds1994，30，5： | 1 |
| 9 | Diffusion processes and structurization in microwave sintering of BaTiO3-SrTiO3 and A1203-Cr2O3 powder systems with complete miscibility显示文摘 | GETMAN O I PANICHKINA V V RADCHENKO P Y SKOROKHOD V V ANDREEVA M G EREMEEV A G KHOLOPTSEV V V | 2009 | Powder Metallurgy and Metal Ceramics2009，48，5： | 1 |
| 10 | A ge-netic algorithm for the allocation of buffer storage capacities in a production line with unreliable machines 显示文摘 | DOLGUI A EREMEEV A KOLOKOLOV A | 2002 | Journal of Mathematical Modeling and Algorithms2002，1，2： | 1 |
| 11 | Evidence for microwave enhanced mass transport in the annealing of nanoporous alumina membranes显示文摘 | BYKOV Y V EGOROV S V EREMEEV A G RYBAKOV K I SEMENOV V E SOROKIN A A GUSEV S A | 2001 | J Mater Sci2001，36，1： | 1 |
| 12 | Enzymatic hydrolysis of keratin containing stock for obtaining protein hydrolysates显示文摘 | Eremeev NL Nikolaev IV Keruchen' ko ID | 2009 | Applied Biochemistry and Microbiology2009，45，6： | 1 |
| 13 | Edge effect in microwave heating of conductive plates 显示文摘 | EGOROV S V EREMEEV A G PLOTNIKOV I V | 2006 | Journal of Physics D: Applied Physics2006，，39： | 1 |
| 14 | Evidence for microwave enhanced mass transport in the annealing of nanoporous alumina membranes 显示文摘 | Bykov Yu V Egorov S V Eremeev A G | 2001 | Journal of Materials Science2001，36，1： | 1 |
| 15 | An Evaluation of Accuracy of Stokes' Series and of Some Attempts to Improve His Theory 显示文摘 | MOLODENSKY M S EREMEEV V F YOURKINA M I | 1962 | Bulletin G6od6sique1962，63，1： | 1 |
| 16 | 4-Aminofurazan-3-earbohydroximie acid halides 显示文摘 | Andrianov V Eremeev A | 1994 | Khim Geterotsikl Soedin1994，26，12： | 1 |
| 17 | Genetic algorithms for a supply management problem: MIP - recombination vs greedy decoder显示文摘 | Borisovsky P Dolgui A Eremeev A | 2007 | Science Direct2007，195，： | 1 |
| 18 | Enzymatic hydrolysis of keratin containing stock for obtaining protein hydrolysates显示文摘 | Eremeev NL Nikolaev IV Kemchen' ko ID ctal | 2009 | Applied Biochemist and Microhiology2009，45，6： | 1 |
| 19 | Synthesis and properties of 4-amino-3-cyanofuraxan显示文摘 | ANDRIANOV V G EREMEEV A V | 1994 | Chemistry of Heterocyelic Compounds1994，30，： | 1 |
| 20 | Evidence for microwave enhanced mass transport in the annealing of nanoporous alumina membranes显示文摘 | BYKOV Y V EGOROV S V EREMEEV A G | 2001 | Journal of Materials Science2001，36，1： | 1 |
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