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| 1 | Role of HSP-90 for increased nNOS-mediated vasodilation in mesenteric arteries in portal hypertension显示文摘AIM:To explore the role of heat shock protein-90 (HSP-90) for nitrergic vasorelaxation in the splanchnic circulation in rats with and without portal hypertension. METHODS: Neuronal nitric oxide synthase (nNOS) and HSP-90 were analyzed by immunofluorescence, western blotting and co-immunoprecipitation in the mesenteric vasculature and isolated nerves of portal-vein-ligated (PVL) rats and sham operated rats. In vitro perfused de-endothelialized mesenteric arterial vasculature was preconstricted with norepinephrine (EC80) and tested for nNOS-mediated vasorelaxation by periarterial nerve stimulation (PNS, 2-12 Hz, 45V) before and after incubation with geldanamycin (specific inhibitor of HSP-90 signalling, 3 μg/mL) or L-NAME (non-specific NOSblocker, 10-4 mol/L). RESULTS: nNOS and HSP-90 expression was significantly increased in mesenteric nerves from PVL as compared to sham rats. Moreover, nNOS and HSP-90 were visualized in mesenteric nerves by immunofluorescence and immunoprecipitation of nNOS co-immunoprecitated HSP-90 in sham and PVL rats. PNS induced a frequencydependent vasorelaxation which was more pronounced in PVL as compared to sham rats. L-NAME and geldanamycin markedly reduced nNOS-mediated vasorelaxation abrogating differences between the study groups. The effect of L-NAME and geldanamycin on nNOS-mediated vasorelaxation was significantly greater in PVL than in sham animals. However, no difference in magnitude of effect between L-NAME and geldanamycin was noted. CONCLUSION: HSP-90 acts as a signalling mediator of nNOS-dependent nerve mediated vascular responses in mesenteric arteries, and the increased nitrergic vasorelaxation observed in portal hypertension is mediated largely by HSP-90. | Lukas Moleda Lars Jurzik Matthias Froh Erwin Gbele Claus Hellerbrand Rainer H Straub Jürgen Schlmerich Reiner Wiest | 2010 | World Journal of Gastroenterology2010，16，15： | 4 |
| 2 | Effect of fiber supplementation on the microbiota in critically ill patients显示文摘AIM:To determine tolerance to fiber supplementation of semi-elemental tube feeds in critically ill patients and measure its effect on colonic microbiota and fermentation.METHODS:Thirteen intensive care unit patients receiving jejunal feeding with a semi-elemental diet for predominantly necrotizing pancreatitis were studied.The study was divided into 2 parts:first,short-term (3-9 d)clinical tolerance and colonic fermentation as assessed by fecal short chain fatty acid(SCFA)concentrations and breath hydrogen and methane was measured in response to progressive fiber supplementation increasing from 4 g tid up to normal requirement levels of 8 g tid;second,4 patients with diarrhea were studied for 2-5 wk with maximal supplementation to additionally assess its influence on fecal microbiota quantitated by quantitative polymerase chain reaction (qPCR)of microbial 16S rRNA genes and Human Intestinal Tract Chip(HITChip)microarray analysis.Nearly all patients were receiving antibiotics(10/13)and acid suppressants(11/13)at some stage during the studies.RESULTS:In group 1,tolerance to progressive fiber supplementation was good with breath hydrogen and methane evidence(P=0.008 and P<0.0001,respectively)of increased fermentation with no exacerbation of abdominal symptoms and resolution of diarrhea in 2 of 4 patients.In group 2 before supplementation,fecal microbiota mass and their metabolites,SCFA,were dramatically lower in patients compared to healthy volunteers.From qPCR and HITChip analyses we calculated that there was a 97%reduction in the predominant potential butyrate producers and starch degraders.Following 2-5 wk of fiber supplementation there was a significant increase in fecal SCFA(acetate 28.4±4.1μmol/g to 42.5±3.1μmol/g dry weight,P=0.01;propionate 1.6±0.5 vs 6.22±1.1,P=0.006 and butyrate 2.5±0.6 vs 5.9±1.1,P=0.04)and microbial counts of specific butyrate producers,with resolution of diarrhea in 3 of 4 patients.CONCLUSION:Conventional management of critically ill patients,which includes the use of elemental diets and broad-spectrum antibiotics,was associated with gross suppression of the colonic microbiota and their production of essential colonic fuels,i.e.,SCFA.Our investigations show that fiber supplementation of the feeds has the potential to improve microbiota mass and function,thereby reducing the risks of diarrhea due to dysbiosis. | Stephen JD O'Keefe Junhai Ou James P DeLany Scott Curry Erwin Zoetendal H Rex Gaskins Scott Gunn | 2011 | World Journal of Gastrointestinal Pathophysiology2011，2，6： | 3 |
| 3 | High Hepatic SCD1 Activity Is Associated with Low Liver Fat Content in Healthy Subjects under a Lipogenic Diet显示文摘 | Günther Silbernagel Marketa Kovarova Alexander Cegan Jürgen Machann Fritz Schick Rainer Lehmann Hans-Ulrich H?ring Norbert Stefan Erwin Schleicher Andreas Fritsche Andreas Peter | 2012 | The Journal of Clinical Endocrinology & Metabolism2012，，12： | 3 |
| 4 | Simple Heuristics for Push and Pull Remanufacturing Policies 显示文摘 | Erwin A Vander Laan Ruud H Teunter | 2006 | European Journal of Operational Research2006，，175： | 1 |
| 5 | The end and the beginning: recoveries from mass extinction显示文摘 | Erwin D H | 1998 | Trends in Ecology and Evolution1998，13，： | 1 |
| 6 | Solid- phase microextrac-tion for the analysis of some alcohols and esters in beer:compari-son with static headspace method 显示文摘 | Henryk H J Krystian W Erwin W etal | 1998 | J Agric Food Chem1998，46，4： | 1 |
| 7 | Lensed fiber-array assembly with individu- al fiber fine positioning in the submicrometer range 显示文摘 | Johan H C van Zantvoort Simon G L Plukker Erwin C A Dekkers | 2006 | IEEE Selected Topics in Quantum Electronics2006，12，05： | 1 |
| 8 | U/Pb zircon geochronology and tempo of the end-Permian mass extinction 显示文摘 | Bowring S A Erwin D H Jin Y | 1998 | Science1998，280，： | 1 |
| 9 | Long term plasticity of ipsilesional vestibular nucleus neurons after unilateral labyrinthectomy显示文摘 | Mathieu B Mohammed H Erwin I | 2003 | J Neurophysiol2003，90，： | 1 |
| 10 | High-efficiency coupling-insensitive transcutaneous power and data transmission via an inductive link 显示文摘 | Clements M Z Erwin S H | 1990 | IEEE Transactions on Biomedical Engineering1990，37，7： | 1 |
| 11 | Candida albicans drug resistance-another way to cope with stress显示文摘 | Richard D C Erwin L Ann R H | 2007 | Microbiology2007，153，10： | 1 |
| 12 | Influence of Mastitis on Properties of Milk X-Fatty Acid Composition显示文摘 | RANDOLPH H E ERWIN R E | 1974 | J Dairy Sci1974，57，： | 1 |
| 13 | Nitrogen elimination from landfill leachatesusing an extra carbon source in subsurface flow constructed wet- lands显示文摘 | Rustige H Ndde Erwin | 2007 | Water Science and Technology2007，56，3： | 1 |
| 14 | Effectiveness of school- based mental health services for children: A 10 -year research re- view显示文摘 | Hoagwood K Erwin H D | 1997 | Journal of Child and Family Studies1997，6，4： | 1 |
| 15 | Autecol- ogy and the filling of ecospace: key metazoan radia- tions 显示文摘 | BAMBACH R K BUSH A M ERWIN D H | 2007 | Palaeontology2007，50，： | 1 |
| 16 | Assessment of various stresses and carbohydrates for their effect on the induction of embryogenesis in isolated wheat microspores显示文摘 | ARI INDRIANTO ERWIN H B ALISHER T | 2003 | Plant Cell2003，74，3： | 1 |
| 17 | The Permo-Triassic extinction显示文摘 | Erwin D H | 1994 | Nature1994，367，： | 1 |
| 18 | Lipopolyaccharide binding protein in acute pancreatitis 显示文摘 | Erwin PJ Lewis H Dolan S | 2000 | Cnt Cate Med2000，28，1： | 1 |
| 19 | Impact of afterschool programs to increase physical activity-ameta- analysis 显示文摘 | Beets M W Beighle A Erwin H E | 2009 | Anerucan Journal of Preventive Medicine2009，36，6： | 1 |
| 20 | U/Pb zircon geochronology and tempo of the end-Permian mass extinction显示文摘 | Bowring S A Erwin D H Jin Y G | 1998 | Science1998，280，： | 1 |
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