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| 1 | Mitochondrial DNA A1555G mutation screening using a testing kit method and its significance in preventing aminoglycoside-related hearing loss显示文摘To report a new screening method for mitochondrial DNA 1555A→G mutation and the results of genotype analysis in 19 maternal inherited deafness pedigrees. Method Five hundred and forty-six non-syndromic neuro-sensory hearing loss patients were tested for 1555A→G mutation using a new compact testing kit, which allows clear distinction between wild type and 1555 A→G mutated mtDNAs. Results Nineteen subjects among the 546 patients (3.48%) were found to carry mtDNA A1555G mutation. The results were confirmed by sequencing in an ABI 3100 Avant sequencer. Conclusions Maternal inherited deafness families are a frequently seen in outpatient group. The detection of mtDNA 1555 A→G mutation with a low cost, ready to use detection kit is needed and suitable in China for large scale screening and preventive testing before usage of aminoglycoside antibiotics. | LIU Xin,1 DAI Pu,1* HUANG Deliang,1 YUAN Huijun,1 LI Weiming,1 YU Fei,1 ZHANG Xin,1 KANG Dongyang,1 CAO Juyang,1 YANG Weiyan,1 HAN Dongyi,1 JIN Zhengce2, GUAN Minxin3 1. Department of Otolaryngology, Chinese PLA General Hospital, Beijing, China2. Weihai Aomaier Gene Technological CO.,LTD.,Weihai,Shandong 264200, China.3. Division and Program in Human Genetics and Center for Hearing and Deafness Research, Cincinnati Children’s Hospital Medical Center, Cincinnati, Ohio, USA | 2006 | Journal of Otology2006，1，1： | 7 |
| 2 | Development of a script of phonemically balanced monosyllable lists of Mandarin-Chinese显示文摘Objectives To develop a set of monosyllable audiometry test material that can be used in speech recognition testing in Mandarin Chinese. Materials and Methods In an effort to improve reliability and efficiency in clinical practice, a set of 30 test lists of 25 monosyllable test items each was designed with consideration of the following: length of the list, phonemical balance (PB), word utility frequency, coverage of common words, and equivalency among lists. The 30 lists contained 750 monosyllabic words and were constructed on the basis of Chinese phoneme distribution probability summarized in the Manual of Acoustic. The occurrence incidence of the 22 consonants, 36 vowels, and 4 tones of the Chinese Mandarin characters in the 30 test lists were calculated. To achieve PB, 489 monosyllables were selected from the 2500 most commonly used Chinese characters and the 4000 most commonly used Chinese phrases to compile the 30 monosyllable test lists using a computer algorithm with manual adjustment. Results Thirty phonemical balanced word lists of common Mandarin Chinese monosyllabic words were compiled. Each list consisted of 25 monosyllables. A total of 489 phonemes (consonants, vowels and tones) were included in the set. Conclusions This set of lists can potentially be used as the basic lists for future development of Mandarin PB monosyllable speech test materials. | JI Fei, CHEN Aiting, ZHAO Yang, XI Xin, HAN Dongyi Departments of Otorhinolaryngology-Head and Neck Surgery, Institute of Otorhinolaryngology, General Hospital of People’s Liberation Army, Beijing 100853, China | 2010 | Journal of Otology2010，5，1： | 4 |
| 3 | Molecular and Epidemiological Characterization of Infant Botulism in Beijing, China显示文摘Laboratory-based pathogen isolation, identification, and toxicity determination were performed on samples from a suspected case of infant botulism. Mice injected with cultures generated from the enema sample and ingested Powered infant formula(PIF) presented typical signs of botulism. Antitoxins to polyvalent botulinum neurotoxins(BoNTs) and monovalent BoNT type B antitoxin had protective effects. Clostridium botulinum isolated from the enema and residual PIF samples were positive for type B toxin. Pulsed-field gel electrophoresis(PFGE) revealed that the two strains of C. botulinum isolated from the two samples produced indistinguishable pulsotypes. These findings confirmed this case of type B infant botulism associated with the ingestion of PIF contaminated by type B C. botulinum spores. | DONG Yin Ping WANG Wei JIANG Tao XU Jin HAN Chun Hui YAN Shao Fei Séamus Fanning LI Ying MA Xiao Chen ZHANG Di ZHAO Yao ZENG Biao LI Feng Qin | 2017 | Biomedical and Environmental Sciences2017，30，6： | 4 |
| 4 | Preclinical Profile and Characterization of the Hepatitis C Virus NS3 Protease Inhibitor Asunaprevir (BMS-650032)显示文摘 | Fiona McPhee Amy K. Sheaffer Jacques Friborg Dennis Hernandez Paul Falk Guangzhi Zhai Steven Levine Susan Chaniewski Fei Yu Diana Barry Chaoqun Chen Min S. Lee Kathy Mosure Li-Qiang Sun Michael Sinz Nicholas A. Meanwell Richard J. Colonno Jay Knipe Paul S | 2012 | Antimicrobial Agents and Chemotherapy2012，，10： | 2 |
| 5 | Urban Modification of Thunderstorms: An Observational Storm Climatology and Model Case Study for the Indianapolis Urban Region*显示文摘 | Niyogi Dev Pyle Patrick Lei Ming Arya S Pal Kishtawal Chandra M Shepherd Marshall Chen Fei Wolfe Brian | 2011 | Journal of Applied Meteorology and Climatology2011，，5： | 2 |
| 6 | Influence of mild hypothermia on vascular endothelial growth factor and infarct volume in brain tissues after cerebral ischemia in rats显示文摘BACKGROUND: It has been demonstrated that mild hypothermia has obvious protective effect on both whole and local cerebral ischemia. However, the definite mechanism is still unclear for the brain protection of mild hypothermia on cerebral edema, inhibiting inflammatory reaction, stabilizing blood brain barrier, etc. OBJECTIVE: To investigate the effect of mild hypothermia on the expression of vascular endothelial growth factor and the infarct volume after cerebral ischemia in rats, and analyze the brain protective mechanism of mild hypothermia. DESIGN: A randomized grouping and controlled animal trial. SETTING: Department of Neurology, People's Hospital of Yunyang Medical College. MATERIALS: Twenty adult male SD rats of clean degree, weighing (250±30) g, were provided by the animal experimental center, School of Medicine, Wuhan University. The kits for SP immunohistochemistry were purchased from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd. METHODS: The experiments were carried out in the laboratory of Department of Neurology, Renmen Hospital of Wuhan University from May to July 2005. ① The 20 rats were divided randomly into normal temperature group (n =10) and mild hypothermia group (n =10). Models of permanent middle cerebral artery occlusion were established with modified nylon suture embolization. The rats were assessed with the Longa standards: 0 point for without nerve dysfunction; 1 for mild neurological deficit (fore claws could no extend completely); 2 for moderate neurological deficit (circling towards the affected side); 3 for severe neurological deficit (tilting towards the affected side); 4 for coma and unconscious; 1-3 points represented that models were successfully established. The rats of the normal temperature group were fed at room temperature, and those in the mild hypothermia group were induced by hypothermia from 2 hours postoperatively, and the rectal temperature was kept at 34-35 ℃ for 72 hours. ② Measurement of infarct volume: All the rats were anesthetized by intraperitoneal injection overdose sodium pentobarbital 7 days postoperatively, and then the heads were cut down to harvest brain. The brain tissues were placed into -20 ℃ refrigerator for 20 minutes, coronal sections of 2 mm were prepared. The infarct sites were not stained, whereas normal brain tissues were stained as red. The infarct volumes were calculated by using MPLAS-500 multimedia color pathological image&word analytical system. ③ Counting positive cells of vascular endothelial growth factor protein: The brains were harvested by cutting heads, then coronal sections of 2 mm were prepared. Routine dehydration, hyalinization, wax immersion and embedding were performed, then the detected with SP immunohistochemistry, the kits were purchased from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd. The cells whose cytoplasm was yellow-brown were positive ones, a single sample as a unit, peri-ischemic site and ischemic core were selected, and the corresponding sites in controlateral hemisphere were taken as controls. Five visual fields were selected from each site to be observed under microscope, the cells were counted, and the average number of positive cells was calculated in each group. The numbers of positive cells were determined with the image analytical apparatus. MAIN OUTCOME MEASURES: Number of the positive cells of vascular endothelial growth factor protein; Infarct volume of rat brain tissue. RESULTS: All the 20 rats were involved in the analysis of results. ① Number of positive cells of vascular endothelial growth factor protein in brain tissue: It was obviously lower in the mild hypothermia group than in the normal temperature group [(24.02±5.05), (36.07±2.69) cells/high power visual field, P < 0.01]. ② Comparison of infarct volume of brain tissue: After MCAO, it was obviously smaller in the mild hypothermia group than in the normal temperature group [(153.25±23.14), (253.45±36.21) mm3, P < 0.01]. CONCLUSION: Mild hypothermia can inhibit the expression of vascular endothelial growth factor and decrease the volume of cerebral infarction. The inhibition of mild hypothermia on the expression of vascular endothelial growth factor may be one of the brain protective mechanisms. | Fei Ye1, Gangming Xi1, Biyong Qin1, Shifeng Wang1, Chengyan Li2 1Department of Neurology, People’s Hospital of Yunyang Medical College, Shiyan 442000, Hubei Province, China 2Department of Neurology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China | 2006 | Neural Regeneration Research2006，1，9： | 2 |
| 7 | A unique deubiquitinase that deconjugates phosphoribosyl-linked protein ubiquitination显示文摘Ubiquitination 调整主人免疫的许多方面并且因此是为传染代理人的一个普通目标。最近的研究表明了细菌的病原体 Legionella pneumophila 的方面受动器家庭的成员攻击不要求主人 ubiquitination 机械的 E1 和 E2 酶的新奇 ubiquitination 机制与 endoplasmic 蜂窝胃联系的几小 GTPases。在这种情况中, ubiquitin 被一项 mono-ADP-ribosyltransferase 活动被 ADP-ribosylation 首先在 Arg 42 激活;中介然后被也住在 SdeA 以内的一项 phosphodiesterase 活动劈开,有到经由一个 phosphoribosyl 连接器的底层蛋白质的丝氨酸残余的 ubiquitin 的附件的伴随物。这里,我们证明方面的效果被 SidJ 反对,一个受动器由位于为方面家庭(SdeC, SdeB 和 SdeA ) 的三个成员编码的地点的基因编码了。SidJ 由劈开连接 phosphoribosylated ubiquitin 到蛋白质底层的 phosphodiester 契约颠倒修改方面的底层的 ubiquitination。SidJ 也显示古典 deubiquitinase 活动但是不要求催化半胱氨酸残余。进一步, SidJ 的这些 deubiquitinase 活动为它在 L 的角色是必要的。pneumophila 感染。最后, SidJ 的活动为高效地在细菌的举起以后在一些小时以内在感染的房间减少许多 ubiquitinated Rab33b 被要求。我们的结果作为工作在方面受动器的活动强加时间的规定的 ubiquitin-deconjugating 酶建立 SidJ。SidJ 可能在表明串联的未来研究是重要的由这唯一的 ubiquitination 调停了,潜在地也在真核细胞的房间调整细胞的过程的。 | Jiazhang Qiu Kaiwen Yu Xiaowen Fei Yao Liu Emesto S Nakayasu Paul D Piehowski Jared B Shaw Kedar Puva Chittaranjan Das Xiaoyun Liu Zhao-Qing Luo | 2017 | Cell Research2017，27，7： | 2 |
| 8 | Enhancement of surface wettability via micro-and nanostructures by single point diamond turning显示文摘Studies on surface wettability have received tremendous interest due to their potential applications in research and industrial processes. One of the strategies to tune surface wettability is modifying surface topography at micro-and nanoscales. In this research, periodic micro-and nanostructures were patterned on several polymer surfaces by ultra-precision single point diamond turning to investigate the relationships between surface topographies at the micro-and nanoscales and their surface wettability. This research revealed that single-point diamond turning could be used to enhance the wettability of a variety of polymers, including polyvinyl chloride(PVC), polyethylene 1000(PE1000), polypropylene copolymer(PP) and polytetrafluoroethylene(PFTE), which cannot be processed by conventional semiconductor-based manufacturing processes. Materials exhibiting common wettability properties(θ≈ 90°) changed to exhibit 'superhydrophobic' behavior(θ > 150°). Compared with the size of the structures, the aspect ratio of the void space between micro-and nanostructures has a strong impact on surface wettability. | Nicolás Cabezudo Jining Sun Behnam Andi Fei Ding Ding Wang Wenlong Chang Xichun Luo Ben B.Xu | 2019 | Nanotechnology and Precision Engineering2019，2，1： | 2 |
| 9 | COI1:An Arabidopsis gene required for jasmonate-regulated defense and fertility显示文摘 | Xie D X Feys B F James S | 1998 | Science1998，280，： | 2 |
| 10 | New aluminum matrix composite with much lower coefficient of thermal expansion and higher strength 显示文摘 | Wang L D Fei W D Jiang L S | 2002 | J Mater Sci Lett2002，21，9： | 2 |
| 11 | Apoptotic effect of ginsenoside Rh2 on human malignant melanoma A375 -S2 cells显示文摘 | Fei XF Wang BX Tashiro S | 2002 | Aeta Pharmacol Sin2002，23，4： | 1 |
| 12 | Integrating forward osmosis into microbial fuel cells for wastewatertreatment, water extraction and bioelectricity generation 显示文摘 | ZHANG Fei BRASTAD K S HE Zhen | 2011 | Environ Sci Technol2011，45，15： | 1 |
| 13 | BambooGDB:a bamboo genome database with functional annotation and an analysis platform显示文摘 | Zhao H S Peng Z H Fei B H | 2014 | Database2014，，： | 1 |
| 14 | How much potassium is in the Earth's core ? New insights from partitioning experiments 显示文摘 | Corgne A Keshav S Fei Y | 2007 | Earth and Planetary Science Letter2007，256，： | 1 |
| 15 | Optimal Teleportation Based on Bell Measurements 显示文摘 | Albeverio S Fei S M Yang W L | 2002 | Phys Rev A2002，66，： | 1 |
| 16 | Third-order cumulant RLS algorithm for nonminimum ARMA systems identification显示文摘 | TOMMY W S TAN Hong-zhou FEI Gou | 1997 | Signal Processing1997，61，1： | 1 |
| 17 | Relationships betweenknowledge inertia, organizational learning and organi- zation innovation 显示文摘 | Liao S Fei W C Liu C T | 2008 | Technovation2008，28，4： | 1 |
| 18 | Fault diagnosis of power transformer based on support vector machine with genetic algorithm 显示文摘 | Fei S W Zhang X B | 2009 | Expert Systems with Applications2009，36，11： | 1 |
| 19 | Mixed maximally entangled states显示文摘 | Li Z G Zhao M J Fei S M | 2012 | Quant Inform Comput2012，12，12： | 1 |
| 20 | Protective effect of chemical stimula- tion of cerebellar fastigial nucleus on stress gastric mucosal injury in rats 显示文摘 | Gao L Fei S Qiao W | 2011 | Life Sciences2011，88，19： | 1 |
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