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| 1 | Enhancing disease resistances of Super Hybrid Rice with four antifungal genes显示文摘A plant expression vector harboring four antifungal genes was delivered into the embryogenic calli of ‘9311’, an indica restorer line of Super Hybrid Rice, via modified biolistic particle bombardment. Southern blot analysis indicated that in the regenerated hygromycin-resistant plants, all the four anti-fungal genes, including RCH10, RAC22, β-Glu and B-RIP, were integrated into the genome of ‘9311’, co-transmitted altogether with the marker gene hpt in a Mendelian pattern. Some transgenic R1 and R2 progenies, with all transgenes displaying a normal expression level in the Northern blot analysis, showed high resistance to Magnaporthe grisea when tested in the typical blast nurseries located in Yanxi and Sanya respectively. Furthermore, transgenic F1 plants, resulting from a cross of R2 homo-zygous lines with high resistance to rice blast with the non-transgenic male sterile line Peiai 64S, showed not only high resistance to M. grisea but also enhanced resistance to rice false smut (a disease caused by Ustilaginoidea virens) and rice kernel smut (another disease caused by Tilletia barclayana). | ZHU HuaChen1, XU XinPing1, XIAO GuoYing2,3, YUAN LongPing2 & LI BaoJian1 1 Biotechnology Research Center, Key Laboratory of Gene Engineering of Ministry of Education, Sun Yat-sen University, Guangzhou 510275, China 2 China National Hybrid Rice R&D Center, Hunan Hybrid Rice Research Center, Changsha 410125, China 3 Institute of Subtropical Agriculture, the Chinese Academy of Sciences, Changsha 410125, China | 2007 | Science China(Life Sciences)2007，50，1： | 6 |
| 2 | Macrophage secretory products induce an inflammatory phenotype in hepatocytes显示文摘AIM:To investigate the influence of macrophages on hepatocyte phenotype and function.METHODS:Macrophages were differentiated from THP-1 monocytes via phorbol myristate acetate stimulation and the effects of monocyte or macrophageconditioned medium on HepG2 mRNA and protein expression determined.The in vivo relevance of these findings was confirmed using liver biopsies from 147 patients with hepatitis C virus(HCV)infection.RESULTS:Conditioned media from macrophages,but not monocytes,induced a transient morphological change in hepatocytes associated with upregulation of vimentin(7.8±2.5-fold,P=0.045)and transforming growth factor(TGF)-β1(2.6±0.2-fold,P<0.001)and downregulation of epithelial cadherin(1.7±0.02-fold,P=0.017)mRNA expression.Microarray analysis revealed significant upregulation of lipocalin-2(17-fold,P <0.001)and pathways associated with inflammation,and substantial downregulation of pathways related to hepatocyte function.In patients with chronic HCV,realtime polymerase chain reaction and immunohistochemistry confirmed an increase in lipocalin-2 mRNA(F0 1.0 ±0.3,F1 2.2±0.2,F2 3.0±9.3,F3/4 4.0±0.8,P= 0.003)and protein expression(F1 1.0±0.5,F2 1.3± 0.4,F3/4 3.6±0.4,P=0.014)with increasing liver injury.High performance liquid chromatography-tandem mass spectrometry analysis identified elevated levels of matrix metalloproteinase(MMP)-9 in macrophageconditioned medium,and a chemical inhibitor of MMP-9 attenuated the change in morphology and mRNA expression of TGF-β1(2.9±0.2 vs 1.04±0.1,P<0.001) in macrophage-conditioned media treated HepG2 cells.In patients with chronic HCV infection,hepatic mRNA expression of CD163(F0 1.0±0.2,F1/2 2.8±0.3,F3/4 5.3±1.0,P=0.001)and MMP-9(F0 1.0±0.4,F1/2 2.8±0.3,F3/4 4.1±0.8,P=0.011)was significantly associated with increasing stage of fibrosis.CONCLUSION:Secreted macrophage products alter the phenotype and function of hepatocytes,with increased expression of inflammatory mediators,suggesting that hepatocytes actively participate in liver injury. | Michelle Melino Victoria L Gadd Gene V Walker Richard Skoien Helen D Barrie Dinesh Jothimani Leigh Horsfall Alun Jones Matthew J Sweet Gethin P Thomas Andrew D Clouston Julie R Jonsson Elizabeth E Powell | 2012 | World Journal of Gastroenterology2012，18，15： | 3 |
| 3 | Enhancement of LTP in aged rats is dependent on endogenous BDNF显示文摘 | Diógenes M J Costenla A R Lopes L V | 2011 | Neuropsychopharmacology2011，36，9： | 1 |
| 4 | The BnALMT1 and malate transporters that enhance the aluminum resistance of plant ceils显示文摘 | Ligaba A Katsuhara M Ryan P R BnALMT2 genes from rape encode | 2006 | Plant Physiology2006，142，3： | 1 |
| 5 | Cadmium toxicity among wildlife in the Colorado Rocky Mountaion显示文摘 | James R L Gene E L John W F | | 0，，13： | 1 |
| 6 | Presence of in Enterococcus faecalis i- solates from pigs in Denmark显示文摘 | Shankar N -Baghdayan A pathogenicity island genes S Willems R | 2006 | J Clin Microbiol2006，44，11： | 1 |
| 7 | 3 locus, occurs in a large percentage of human breast cancers显示文摘 | Dammann R Yang G Pfeifer GP Hypermethylation of the CpG island of Ras association domain family 1A (RASSF1A) a putative tumor suppressor gene from the 3p21 | 2001 | Cancer Res2001，61，7： | 1 |
| 8 | Phylogenetic analysis of the hyperthermophilic pink Filament community in octopus spring,Yellowstone National Park 显示文摘 | Reysenbach A L Gene S W Norman R P | 1994 | ApplEaviron Microbioi1994，60，： | 1 |
| 9 | Molecular phylogeny of Sporothrix schenckii 显示文摘 | Marimon R Gene J Cano J | 2006 | J Clin Mierobiol2006，44，9： | 1 |
| 10 | Population dynamics of an asexual brine shrimp Artemia population显示文摘 | GENE H MacDONALD BROWNE R A | 1989 | J Exp Mar Biol Ecol1989，133，： | 1 |
| 11 | The glucocorticoid re- is associated with improved lung function in asthmatic subjects treated with inhaled corticosteroids 显示文摘 | Hawkins GA Lazarus R Smith ceptor heterocomplex gene STIP1 RS | 2009 | J Allergy Clin lmmunol2009，123，： | 1 |
| 12 | Tritici-repentis and Implications for QoI Resistance 显示文摘 | SIEROTZKI H FREY R J Cytochrome b Gene Sequence and Structure of Pyrenophora teres and P | 2007 | Pest Mang Sci2007，63，： | 1 |
| 13 | Air-assisted sprayer adapted for precision horticulture : Spray patterns and dep- osition assessments in small-sized citrus canopies显示文摘 | Lav R Knot Reza Ehsani Gene Albrigo | 2012 | Biosystems Engineering2012，113，1： | 1 |
| 14 | Molecular phylogeny of Sporothrix schenckii显示文摘 | Marimon R Gene J Cano J | 2006 | J Clin Microbiol2006，44，9： | 1 |
| 15 | Inhibited flammability and surface inactivation of wood irradiated by low energy hydrogen ion showers (LEHIS) 显示文摘 | Gene Q Blantocas Philip Edward R Mateum Ross William M Orille | 2007 | Nuclear Instruments and Methods in Physics Research B2007，259，2： | 1 |
| 16 | Air-assisted sprayer adapted for precision horticulture: Spray patterns and deposition assessments in small-sized citrus canopies显示文摘 | Lav R Khot Reza Ehsani Gene Albrigo | 2012 | Biosystems Engineering2012，13，1： | 1 |
| 17 | In vitro antifungal sus- ceptibilities of five species of Sporothrix 显示文摘 | Marimon R Serena C Gene J | 2008 | Antimicrob A- gents Chemother2008，52，2： | 1 |
| 18 | Molecular phylogeny of Sporothrix schenckii 显示文摘 | Marimon R Gene J Cano J | 2006 | J Clin Microbiol2006，44，9： | 1 |
| 19 | In vitro antifungal activity of extracts and neolignans from Piper regnellii against dermatophytes显示文摘 | Koroishi Andrea M Foss Simone R Cortez Diógenes A G | 2008 | Journal of Ethnopharmacology2008，117，2： | 1 |
| 20 | 显示文摘 | Van der Salm TPM Bouwer R van Dijk AJ et aL Stimulation of scion bud release by rol gene transformed rootstocks of Rosa hybrida L | 1998 | J Exp Bot1998，49，： | 1 |
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