|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Effect of adipose-derived mesenchymal stem cells on hepatocellular carcinoma: In vitro inhibition of carcinogenesis显示文摘AIM To investigate the effect of adipose-derived mesenchymal stem cells(ADMSCs)and their conditioned media(CM) on hepatocellular carcinoma(HCC) cell tumorigenesis.METHODS The proliferation rate of HepG2 and PLC-PRF-5 HCC cancer cells was measured using the trypan blue exclusion method and confirmed using the cell-counting kit8(commonly known as CCK-8) assay. Apoptosis was detected by flow cytometry using annexin V-FITC. Protein and mRNA expression was quantified by ELISA and real time PCR, respectively. Migration and invasion rates were performed by Transwell migration and invasion assays. Wound healing was examined to confirm the data obtained from the migration assays.RESULTS Our data demonstrated that when co-culturing HCC cell lines with ADMSCs or treating them with ADMSC CM, the HCC cell proliferation rate was significantly inhibited and the apoptosis rate increased. The decreased proliferation rate was accompanied by an upregulation of P53 and Retinoblastoma mRNA and a downregulation of c-Myc and hTERT mRNA levels. More notably, ADMSCs and their CM suppressed the expression of the two important markers of HCC carcinogenicity, alpha-fetoprotein and Des-gamma-carboxyprothrombin. In addition, the migration and invasion levels of HepG2 and PLC-PRF-5 cells significantly decreased, potentially through increased expression of the tissue inhibitor metalloproteinases TIMP-1, TIMP-2 and TIMP-3.CONCLUSION These findings shed new light on a protective and therapeutic role for ADMSCs and their CM in controlling HCC invasiveness and carcinogenesis. | Rim Serhal Nagib Saliba George Hilal Mayssam Moussa Ghada S Hassan Oula El Atat Nada Alaaeddine | 2019 | World Journal of Gastroenterology2019，25，5： | 6 |
| 2 | Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) supports adhesion and migration of mesenchymal stem cells and tenocytes显示文摘AIM: To establish the potential of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) as a material for tendon repair. METHODS: The biocompatibility of PHBHHx with both rat tenocytes (rT) and human mesenchymal stem cells (hMSC) was explored by monitoring adhesive characteristics on films of varying weight/volume ratios coupled to a culture atmosphere of either 21% O2 (air) or 2% O2 (physiological normoxia). The diameter and stiffness of PHBHHx films was established using optical coherence tomography and mechanical testing, respectively. RESULTS: Film thickness correlated directly with weight/volume PHBHHx (r2 = 0.9473) ranging from 0.1 mm (0.8% weight/volume) to 0.19 mm (2.4% weight/volume). Film stiffness on the other hand displayed a biphasic response which increased rapidly at values > 1.6% weight/volume. Optimal cell attachment of rT required films of ≥ 1.6% and ≥ 2.0% weight/volume PHBHHx in 2% O2 and 21% O2 respectively. A qualitative adhesion increase was noted for hMSC in films ≥ 1.2% weight/volume, becoming significant at 2% weight/volume in 2% O2. An increase in cell adhesion was also noted with ≥ 2% weight/volume PHBHHx in 21% O2. Cell migration into films was not observed. CONCLUSION: This evaluation demonstrates that PHBHHx is a suitable polymer for future cell/polymer replacement strategies in tendon repair. | Alex J Lomas George GQ Chen Alicia J El Haj Nicholas R Forsyth | 2012 | World Journal of Stem Cells2012，4，9： | 2 |
| 3 | Day-night variations in blood levels of nitric oxide,T-TFPI,and E-selectin显示文摘 | Kanabrocki EL George M Hermida RC | 2001 | Clin Appl Thromb Hemost2001，7，4： | 1 |
| 4 | Mucosal vaccine delivery of antigens tightly bound to an adjuvant particle made from food-grade bacteria显示文摘 | Maarten L. van Roosmalen Rolf Kanninga Mohamed El Khattabi Jolanda Neef Sandrine Audouy Tjibbe Bosma Anneke Kuipers Eduard Post Anton Steen Jan Kok Girbe Buist Oscar P. Kuipers George Robillard Kees Leenhouts | 2005 | Methods2005，，2： | 1 |
| 5 | The adaptor protein paxillin is essential for normal development in the mouse and is a critical transducer of fibronectin signaling显示文摘 | Hagel M George EL Kim A | 2002 | Mol Cell Biol2002，22，： | 1 |
| 6 | A novel ga- lacto-oligosaccharide mixture increases the bifidobacterial population number in a continuous in vitro fermentation sys- tem and in the proximal colonic contents of pigs in vivo 显示文摘 | George T Athanasios K G Jennifer M G el al | 2005 | The American Society for :utritional Sciencese2005，135，7： | 1 |
| 7 | Shape retrieval using triangle-area representation and dynamic space warping显示文摘 | Naif Alajlan Ibrahim El Rube Mohamed S. Kamel George Freeman | 2007 | Pattern Recognition2007，，7： | 1 |
| 8 | Osteorotegerin is a risk factor forprogressive athemsclerosis and cardiovscular disease 显示文摘 | Stcfan K George S George W el al | 2004 | Circulation2004，109，18： | 1 |
| 9 | Efficacy of amiodarone for the termination of persistent atrial fibrillation 显示文摘 | George EK Nikos El Marios CS | 1999 | American Journal of Cardiology1999，83，3： | 1 |
| 10 | Identification of radiation- induced expression changes in norimmortalized human T cells 显示文摘 | Pogosova- Agadjanyan EL Fan W Georges GE | 2011 | Radiat Res2011，175，2： | 1 |
| 11 | The adaptor protein paxillin is essential for normal development in the mouse and is a critical transducer of fibronectin signaling显示文摘 | Hagel M George EL Kim A | 2002 | Mol Cell Biol2002，22，3： | 1 |
| 12 | A process for instituting best practice in the in- tensive care unit 显示文摘 | George EL Tuite P | 2008 | Indian J Crit Care Med2008，12，2： | 1 |
| 13 | The role of the clinical nurse specialist in facilitating evidence-based practice within a university setting显示文摘 | Uite PK George EL | 2010 | Crit Care Nurs Q2010，33，2： | 1 |
| 14 | Disruption of dopamine D, receptor gene expression attenuates alcohol - seeking behavior显示文摘 | El - Ghundi M George SR Drago J | 1998 | Eur J Pharmacol1998，353，23： | 1 |
| 15 | Recurrence of non‐alcoholic steatohepatitis and cryptogenic cirrhosis following orthotopic liver transplantation in the context of the metabolic syndrome显示文摘 | Mazen M. El Atrache Marwan S. Abouljoud George Divine Atsushi Yoshida Dean Y. Kim Marwan M. Kazimi Dilip Moonka Mary A. Huang Kim Brown | 2012 | Clin Transplant2012，，5： | 1 |
| 16 | Day- night variations in blood levels of nitric oxide,T - TFPI,and E - seleetin显示文摘 | Kanabrocki EL George M Hermida RC | 2001 | Clin Appl Thromb Hemost2001，7，4： | 1 |
| 17 | The adaptor protein paxillin is essential for normal development in the mouse and is a critical transducer of fibronectin signaling显示文摘 | Hagel M George EL Kim A | 2002 | Mol Cell Biol2002，22，3： | 1 |
| 18 | Intelligent GIS a Fundamental Change in the Way Primary and Secondary Equipment is Combined显示文摘 | Georg Sche Fred Engler Alfred Jaussl el al | 1996 | ABB Review1996，，8： | 1 |
| 19 | Troponin targets cardiac injury显示文摘 | George EL Shatzer M | 2007 | Nursing Critical Care2007，2，： | 1 |
| 20 | Urinary monocyte chemoattractant protein- 1 (MCP-1)is a marker of active renal vasculitis显示文摘 | Tam FW Sanders JS George A el al | 2004 | Nephrol Dial Transplant2004，19，11： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员