|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 离体肝脏常温下保存的实验研究显示文摘为了探索常温下(22℃)保存离体肝脏的可能性,本实验以HTK液为基础,氟碳乳剂为氧载体,脂肪乳剂为能量,常温下充氧持续灌注离体猪肝门静脉。定时取灌注液测量肝生化指标,观察肝细胞线粒体大小及密度。发现含氧载体灌注液中氨浓度下降,尿素浓度升高,线粒体损伤较轻;加入脂肪乳剂则能维持葡萄糖的浓度。各项指标与对照组比较差异有显著意义。说明利用氧载体(氟碳乳剂)为离体肝提供足够的氧,同时给予足够的能量(脂肪乳剂)时,离体肝脏就能在较高温度下较长时间(40小时)地维持它的主要解毒功能——摄取氨合成尿素,同时亦可维持肝细胞线粒体的稳定性。可见常温下保存离体肝脏是可能的。 | 裘华德 陈义发 Gruenert A Mueler I Schuh S | 1996 | 中华外科杂志1996，34，9： | 6 |
| 2 | Sensitivity and specificity of different staining methods to monitor apoptosis induced by oxidative stress in adherent cells显示文摘Objective To study the sensitivity and specificity of different staining methods to monitor apoptosis induced by oxidative stress in adherent cells.Methods Sensitivity and specificity of several common methods for apoptosis determination were evaluated (Apo2.7-expression, Annexin V-binding, TUNEL-reaction, poly-(ADP-ribose)-polymerase-(PARP) cleavage and single-stranded-DNA (ssDNA) staining). Apoptosis was induced by oxidative stress generated by hydrogen peroxide in 3 cultured cells types growing as adherent monolayer (MiaPaCa-2, Hep-G2 and human skin fibroblasts), necrosis was induced by depletion of cellular ATP using sodium azide. Cells positively stained by the respective apoptosis assay were quantified and alterations of cell morphology were monitored by fluorescence microscopy. The date was analyzed by one-way analysis of variance and significance test of correlation coefficient.Results One hour after apoptosis induction significant cell fractions were positively stained for ssDNA (33% with MiaPaCa-2 cells, 35% with Hep-G2 cells, 56% with human skin fibroblasts). PARP-cleavage was less sensitive compared to the ssDNA-staining. Apo2.7-expression, Annexin V-binding and TUNEL-reaction were not applicable to detect early apoptosis induced by oxidative stress (below 2 hours), but were efficiently monitoring late apoptosis. Specificity of ssDNA-staining was complete with each cell type even 4 hs after induction of necrosis by the highest sodium azide concentration. In contrast, the same experimental conditions resulted in 50%-90% positively stained necrotic cells by using Apo2.7-expression, TUNEL-reaction or AnnexinV-binding. Surprisingly, specificity of PARP-cleavage was highly depending on the respective cell type.Conclusions Our study prove that among the five methods investigated only ssDNA-staining allowed to completely differentiate apoptosis from necrosis, and is thus suitable to reliably detect early as well as late apoptosis. Therefore, the ssDNA-staining may be used as reference method to clearly identify apoptosis induced by oxidative stress in adherent cells. The TUNEL-reaction, annexin-V-binding and Apo-2.7-expression may be used to quantify the number of apoptotic and necrotic cells especially at later stages but without discrimination of apoptosis and primary or secondary necrosis. | 赵进顺 Alexandra Schmid-Kotsas Hans-Juergen Gross Adolf Gruenert Max G. Bachem | 2003 | Chinese Medical Journal2003，，12： | 4 |
| 3 | The 180-degree perforator-based propeller flap for soft tissue coverage of the distal,lower extremity:a new method to achieve reliable coverage of the distal lower extremity with a local,fasciocutaneous perforator flap显示文摘 | Jakubietz RG Jakubietz MG Gruenert JG | 2007 | Aun Plast Surg2007，59，6： | 1 |
| 4 | Palmar and dorsal fixed-angle plates in AO C-type fractures of the distal radius:is there an advantage of palmar plates in the long term显示文摘 | Jakubietz MG Gruenert JG Jakubietz RG | 2012 | J Orthop Surg Res2012，7，1： | 1 |
| 5 | A randomised clinical study comparing palmar and dorsal fixed-angle plates for the internal fixation of AO C-type fractures of the distal radius in the elderly显示文摘 | Jakubietz RG Gruenert JG Kloss DF | 2008 | J Hand Surg Eur Vol2008，33，5： | 1 |
| 6 | XPS and FTIR Study of Ru/Al2O3 and Ru/TiO2 catalysts: Reduction characteristics and interaction with a methaneoxygen mixture 显示文摘 | Elmasides C Kondarides D I Gruenert W | 1999 | J Phys Chem B1999，103，26： | 1 |
| 7 | Neutrophil recruitment by human IL -17 via C -X -C chemokine release in the airways显示文摘 | Laan M Cui ZH Hoshino H Lotvall J Sjosrtand M Gruenert DC Skoogh BE Linden A | 1999 | J Immunol1999，162，4： | 1 |
| 8 | Airway epithelial cell wound repair mediated by α-Dystroglycan显示文摘 | WHITE SR WOJCIK KR GRUENERT D | 2001 | Am J Respir Cell Mol Biol2001，24，2： | 1 |
| 9 | The 180-degree perforator-based propeller flap for soft tissue coverage of the distal, lower extremity: a new method to achieve reliable coverage of the distal lower extremity with a local, fasciocutaneous perforator flap 显示文摘 | Jakubietz RG Jakubietz MG Gruenert JG | 2007 | Ann Plast Surg2007，59，6： | 1 |
| 10 | A randomisedclinical study comparing palmar and dorsal fixed-angle plates forthe internal fixation of AO C-type fractures of the distal radius inthe elderly 显示文摘 | Jakubietz R G Gruenert J G Kloss D F | 2008 | J Hand Surg Am2008，33，5： | 1 |
| 11 | CFTR channels in immortalized human airway cells 显示文摘 | Haws C Krouse M E Xia Y Gruenert DC Wine J J | 1992 | Am J Physiol1992，263，： | 1 |
| 12 | Reconstruction of soft tissue defects of the Achilles tendon with rotation flaps, pedicled propeller flaps and free perforator flaps 显示文摘 | Jakubietz RG Jakubietz DF Gruenert JG | 2010 | Microsurgery2010，30，8： | 1 |
| 13 | Airway epithelial cell wound repair mediated by a-dystroglycan 显示文摘 | White S R Wojcik K R Gruenert D | 2001 | Am J Respir Cell Mol Biol2001，24，： | 1 |
| 14 | The 180-degree perforator-based propeller flap for soft tissue coverage of the distal, lower extremity: a new method to achieve reliable coverage of the distal lower extremity with a local, fasciocutaneous perforator flap 显示文摘 | Jakubietz RG Jakubietz MG Gruenert JG | 2007 | Ann Plast Surg2007，59，6： | 1 |
| 15 | Development and characterization of SV40 immortalized rat submandibular acinar cell lines 显示文摘 | Quissell DO Barzen KA Gruenert DC | 1997 | In Vitro Cell Dev-Animal1997，33，： | 1 |
| 16 | The 180-degree perforator-based propeller flap for soft tissue coverage of the distal,lower extremity:a new method to achieve reliable coverage of the distal lower extremity with a local,fasciocutaneous perforator flap显示文摘 | JAKUBIETZ R G JAKUBIETZ M G GRUENERT J G | 2007 | Ann Plast Surg2007，59，6： | 1 |
| 17 | Culture and transformation of human airway epithelial cells显示文摘 | Gruenert DC Finkbeiner WE Widdicombe JH | 1995 | Am J Physiol1995，268，3： | 1 |
| 18 | Culture and transformation of human airway epithelial cells 显示文摘 | Gruenert DC Finkbeiner WE Widdicombe JH | 1995 | Am J Physiol''1995，268，3： | 1 |
| 19 | Rhinovirus disrupts the barrier function of polarized airway epithelial cells 显示文摘 | Sajjan U Wang Q Zhao Y Gruenert DC | 2008 | Am J Respir Crit Care Med2008，178，12： | 1 |
| 20 | Palmar and dorsal fixed- angle plates in ao c-type fractures of the distal radius: is there an advantage of palmar plates in the long term显示文摘 | Jakubietz MG Gruenert JG Jakubietz RG | 2012 | J Orthop Surg Res2012，7，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员