|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Heterologous expression,chaperone mediated solubilization and purification of parasitic nematode-specific growth factor-like protein of Setaria digitata显示文摘Objective:To clone,express and purify a putative parasitic nematode specific protein of Setaria digitata(S.digitata),filarial nematode that infects livestock and cause significant economic losses in Far East and Asia to he used for structural and functional analyses.Methods:To characterize uneharacterized gene of,S.digitata(SDUG),the herterologous expression of SDUG was carried out in the pET[cloned into pET45b(+)]expression system initially and co-expression of SDUC using chaperoiie plasmids pG-KJE8,pGro 7,pKJE7,pG-Tf2 and pTf16 containing chapcrone proteins of dnaK-dnaJ-grpE-groES-gro-E,groES-groEL,dnaK-dnaJ-grpE,groES-groEL-tig,and tig respectively,was carried out subsequently.Results:Expression of SDUG was seen when Escherichia coli strain BI.21(DE3)is used,while concentrating protein largely into the insoluble fraction.The co-expression of SDUG using chaperoiie plasmid mediated system indicated a significant increase of the protein in the soluble fraction.Of the chaperon plasniid sets,the highest amount of recombinant SDUP in the soluble fraction was seen when pGro7 was used in the presence of2 mg/mL L-arabinosc and 0.6M IPTG concentration in the culture medium and for 3 h of incubation at the temperature of 28℃.Recombinant SDUG was purified both from soluble and insoluble fractions using Ni affinity chromatography.SDS-PAGE and western blot analyses of these proteins revealed a single band having expected size of^24 kDa.Conclusions:SDUG seems to be more aggregate-prone and hydrophobic in nature and such protein can make soluble by correct selecting the inducer concentrations and induction temperature and its duration. | W.WP.Rodrigo R.S Dassanayake E.H.Karunanayake Y.I.N.Silva Gunawardene O.VDS.J.Weerasena | 2014 | Asian Pacific Journal of Tropical Medicine2014，7，2： | 2 |
| 2 | Changes inresidual volume relative to vital capacity and total lung capacityafter arthrodesis of the spine in patients who have adolescent idio-pathic scoliosis显示文摘 | Upadhyay SS Ho EK Gunawardene WM | 1993 | J Bone Joint Surg Am1993，75，1： | 1 |
| 3 | The Elehera gem field in central Sri Lanka显示文摘 | Gunawardene M Rupasinghe M S | 1986 | Gems & Gemology1986，22，2： | 1 |
| 4 | Reddish-brown sapphires from Umba Valley, Tanzania显示文摘 | Gunawardene M | 1984 | Journal of Gemmology1984，19，2： | 1 |
| 5 | Regulation of cterminaland intact FGF-23 by dietary phosphatein men and women显示文摘 | Burnett S A M Gunawardene S C Bringhurst F R | 2006 | Journal of Bone and mineral Research2006，21，8： | 1 |
| 6 | Effects of lowdose prednisone on bone metabolism 显示文摘 | TON FN GUNAWARDENE SC LEE H | 2005 | J Bone Miner Res2005，20，3： | 1 |
| 7 | ITS-2 secondary structures and phylogeny of Anopheles culicifacies species 显示文摘 | Dassanayake R S Gunawardene Y I Silva B D | 2008 | Bioinformation2008，2，： | 1 |
| 8 | Correlates of ant ( Hymenoptera: Formicidae ) and tree species diversity in Sri Lanka 显示文摘 | GUNAWARDENE N R MAJER J D EDIRISINGHE J P | 2012 | Myrmecological News2012，17，： | 1 |
| 9 | The shrimpFAMeT cDNA is encoded for a putative enzyme involved in themethylfarnesoate(MF)biosynthetic pathway and is temporally ex-pressed in the eyestalk of different sexes显示文摘 | Silva Gunawardene Y I N Chow B K He J G | 2001 | Insect Biochem MolBiol2001，31，11： | 1 |
| 10 | Func tion and cellular localization of farnesoic acid O-meth yltransferase(FAMeT) in the shrimp, Metapenaeus en sis显示文摘 | Gunawardene Y I Tobe S S Bendena W G | 2002 | European Journal of Biochemistry2002，269，14： | 1 |
| 11 | Effects of low-dose prednisone on bone metabolism显示文摘 | Ton FN Gunawardene SC Lee H | 2005 | J Bone Miner Res2005，20，3： | 1 |
| 12 | Pre-operative par- ental anxiety显示文摘 | Thompson N Irwin MG Gunawardene WM | 1996 | Anaesthesia1996，51，11： | 1 |
| 13 | Absence of Wolbachia endobaeteria in Sri Lankan isolates of the nematode parasite of animals Setaria digitata 显示文摘 | Voronin D Abeykoon AMLL Gunawardene YIS | 2015 | Vet Parasitol2015，207，34： | 1 |
| 14 | Comparison of balloon catheter ablation technologies for pulmonary vein isolation:the laser versus cryo study显示文摘 | Bordignon S Chun KR Gunawardene M | 2013 | J Cardiovasc Electrophysiol2013，24，9： | 1 |
| 15 | Regulation of cterminal and intact FGF-23 by dietary phosphate in men and women显示文摘 | Burnett SM Gunawardene SC Bringhurst FR | 2006 | J Bone Miner Res2006，21，8： | 1 |
| 16 | Efifects of low-dose prednisone on bonemetabolism显示文摘 | Ton FN Gunawardene SC Lee H | 2005 | J Bone Miner Res2005，0，46： | 1 |
| 17 | Inhibition of nuclear factor kappaB induces apoptosis following treatment with tumor necrosis factortt and antioxidant in human postate cance cells显示文摘 | Gunawarden K Murray DK Swope RE | 2002 | Cancer Detect Prey2002，26，3： | 1 |
| 18 | The role of root border cells in plant defense显示文摘 | H AWES M C GUNAWARDEN A MIYASAK A S | 2000 | Trends in Plant Science2000，5，： | 1 |
| 19 | A prospective randomized single-center study on the risk of asymptomatic cerebral lesions comparing irrigated radiofrequency current ablation with the cryoballoon and the laser balloon显示文摘 | Schmidt B Gunawardene M Krieg D | 2013 | J Cardiovasc Electrophysiol2013，24，： | 1 |
| 20 | Inhibition of nuclear factor kB induces apoptosis following treatment with tumor necrosis factor-α and antioxidant in human postate cance cells显示文摘 | Gunawarden K Murray DK Swope RE | 2002 | Cancer Detect Prey2002，26，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员