|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 犬粪便棘球绦虫抗原的双抗体夹心酶联免疫吸附试验检测法建立与应用显示文摘目的研究双抗体夹心酶联免疫吸附检测犬粪便棘球绦虫虫体抗原的方法并评价其在实际工作中的应用价值。方法实验犬12只,分实验组和对照组,实验组用活化绵羊源原头蚴(G1型)作人工感染;感染后0~8周内收集粪便,56d宰杀实验犬,收集成虫;制备成虫抗原和虫体代谢/分泌抗原,蛋白酶K处理抗原并分别免疫成年绵羊和新西兰白兔;绵羊抗血清用80%饱和硫酸铵沉淀、透析,兔抗血清过IgG亲和层析柱,分别获得纯化抗体;观察犬粪便在PBS液中-80℃冷冻3d或在含1%Formalin的PBS液中70℃~80℃加热8h的处理方法对检出结果的影响。运用该方法对四川省甘孜县达通玛区4个乡的580只家犬吡喹酮每6月一次驱虫前后的粪抗原进行监测。结果试验系统的敏感性为92.3%,特异性为80%;粪抗原从感染后的第2~3周可检出;两种样品处理方法都可以灭活虫卵但不影响检出效果;检测方法可同时用于对细粒棘球绦虫和多房棘球绦虫感染的诊断;达通玛区家犬粪抗原阳性率从2000年的50%下降到2005年的17%。结论检测方法具有较高的特异性和敏感性;推荐粪便用加热的方法处理可以灭活虫卵以防止生物污染;该方法可以在基层推广应用。 | 黄燕 Heath D David Antone J Christine 邱加闽 王谦 | 2008 | 预防医学情报杂志2008，24，6： | 22 |
| 2 | Short-term endpoints of conventional versus laparoscopic-assisted surgery in patients with colorectal cancer (MRC CLASICC trial): multicentre, randomised controlled trial显示文摘 | Pierre J Guillou Philip Quirke Helen Thorpe Joanne Walker David G Jayne Adrian MH Smith Richard M Heath Julia M Brown | 2005 | The Lancet . 2005 (9472)2005，，9472： | 6 |
| 3 | Short-term endpoints of conventional versus laparoscopic-assisted surgery in patients with colorectal cancer (MRC CLASICC trial): multicentre, randomised controlled trial显示文摘 | Pierre J Guillou Philip Quirke Helen Thorpe Joanne Walker David G Jayne Adrian MH Smith Richard M Heath Julia M Brown | 2005 | The Lancet2005，，9472： | 4 |
| 4 | Short-term endpoints of conventional versus laparoscopic-assisted surgery in patients with colorectal cancer (MRC CLASICC trial): multicentre, randomised controlled trial显示文摘 | Pierre J Guillou Philip Quirke Helen Thorpe Joanne Walker David G Jayne Adrian MH Smith Richard M Heath Julia M Brown | 2005 | 2005 (9472)2005，，9472： | 2 |
| 5 | Short-term endpoints of conventional versus laparoscopic-assisted surgery in patients with colorectal cancer (MRC CLASICC trial): multicentre, randomised controlled trial显示文摘 | Pierre J Guillou Philip Quirke Helen Thorpe Joanne Walker David G Jayne Adrian MH Smith Richard M Heath Julia M Brown | 2005 | The Lancet2005，，9472： | 2 |
| 6 | Construction of a recombinant or virus that expresses an Echinococcus granulosus vaccine antigen from a novel genomic insertion site 显示文摘 | Marsland BJ Tisdall D J Heath DD | 2003 | Arch Virol2003，148，3： | 1 |
| 7 | C;enome wide association study identifies variants at CLU and CR1 associated with Alzhei mer's disease显示文摘 | Lambert J C Heath S Even G | 2009 | Nat Genet2009，41，10： | 1 |
| 8 | On quasi-orthogonal signatures for CDMA systems显示文摘 | Heath R W Strohmer T Paulraj A J | 2002 | IEEE Trans Inform Theory2002，52，3： | 1 |
| 9 | What is the Value of Limited Feedback for MIMO Channels显示文摘 | Love D J Heath R W J Santipach W | 2004 | IEEE Commun Magazine2004，42，10： | 1 |
| 10 | C60:Buckminster- fullerene显示文摘 | Kroto H W Heath J R O'Brien S C | 1985 | Nature1985，318，： | 1 |
| 11 | Continuous flow residence time distribution function characterization显示文摘 | Haas C N Joffe J Heath M S | | 0，，02： | 1 |
| 12 | Reproducibility of serology assays for pandemic influenza H1N1:collaborative study to evaluate a candidate WHO international standard显示文摘 | WOOD J M MAJOR D HEATH A | | 0，，02： | 1 |
| 13 | Block diagonal-ization for muhi-user MIMO with other-cell interference 显示文摘 | SHIM S KWAK J S HEATH R W | 2008 | IEEE Transactions on Wireless Communications2008，7，7： | 1 |
| 14 | An infrared thermographic study of surface temperature in relation to external thermal stress in the Mongolian gerbil,Meriones unguiculatus显示文摘 | Klir J J Heath J E Bennani N | 1990 | Comparative Biochemistry&Physiology Part A Physiology1990，96，1： | 1 |
| 15 | CT angiography with volume rendering for quantifying vascular stenoses: in vitro validation of accuracy 显示文摘 | Kuszyk BS Heath DG Johnson PT Eng J Fishman EK | 1999 | MR Am J Roentgenol1999，173，2： | 1 |
| 16 | Block diagonalization for multi-user MIMO with other-cell interference显示文摘 | SHIM S KWAK J S HEATH R W | 2008 | IEEE Trans Wireless Commun2008，7，7： | 1 |
| 17 | Designing structured tight frames via an alternating projection method显示文摘 | Tropp J A Dhillon I S Heath R W | 2005 | IEEE Trans Inform Theory2005，51，1： | 1 |
| 18 | High Speed Storage Area Networks Using a Fiber显示文摘 | Heath J R Yakutis P J | 2000 | Channel Arbitrated Loop Interconnect Network IEEE2000，14，2： | 1 |
| 19 | Development and validationof a BEAMnrc component for accurateMonte Carlo modeling of the varian dynamicmillenium multileaf collimator显示文摘 | Heath E Seuntjens J | 2003 | Phys MedBiol2003，48，24： | 1 |
| 20 | A collaborative study on DNA quantitation in biological products显示文摘 | ROBERTSON J S HEATH A B | 1995 | Biologicals1995，23，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员