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82篇 您的检索式：作者名="Helbig J"

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	题名	作者	年代	出处	被引量
1	Osteopontin increases hepatocellular carcinoma cell growth in a CD44 dependant manner显示文摘AIM:To investigate the role of osteopontin(OPN) and its splice variants in the proliferation of hepatocellular carcinoma(HCC).METHODS:The expression of OPN variants in HCC cell lines as well as HCC tissue samples and nontumour tissue was studied using polymerase chain reaction.OPN variant cDNAs were cloned into a mammalian expression vector allowing both transient expression and the production of stable OPN expressing cell lines.OPN expression was studied in these cells using Western blotting,immunofluoresnce and enzyme linked immunosorbent assay.A CD44 blocking antibody and siRNA targeting of CD44 were used to examine the role of this receptor in the OPN stimulated cell growth observed in culture.Huh-7 cells stably expressing either OPN-A,-B or-C were injected subcutaneously into the flanks of nude mice to observe in vivo tumour growth.Expression of OPN mRNA and protein in these tumours was examined using reverse transcriptionpolymerase chain reaction and immunohistochemistry.RESULTS:OPN is expressed in HCC in 3 forms,the full length OPN-A and 2 splice variants OPN-B and-C.OPN variant expression was noted in HCC tissue as well as cognate surrounding cirrhotic liver tissue.Expression of these OPN variants in the HCC derived cell line Huh-7 resulted in secretion of OPN into the culture medium.Transfer of OPN conditioned media to na ve Huh-7 and HepG2 cells resulted in significant cell growth suggesting that all OPN variants can modulate cell proliferation in a paracrine manner.Furthermore the OPN mediated increase in cellular proliferation was dependent on CD44 as only CD44 positive cell lines responded to OPN conditioned media while siRNA knockdown of CD44 blocked the proliferative effect.OPN expression also increased the proliferation of Huh-7 cells in a subcutaneous nude mouse tumour model,with Huh-7 cells expressing OPN-A showing the greatest proliferative effect.CONCLUSION:This study demonstrates that OPN plays a significant role in the proliferation of HCC through interaction with the cell surface receptor CD44.Modulation of this interaction could represent a novel strategy for the control of HCC.	Renee J Phillips Karla J Helbig Kylie H Van der Hoek Devanshi Seth Michael R Beard	2012	World Journal of Gastroenterology2012，18，26：	12
2	Occurrence and distribution of sequence types among Legiortella pneumophila strains isolated from patiens in Germany: common features and differences to other regions of the world显示文摘	BORCHARDT J HELBIG J H LUCK P C	2008	Eur J Clin microbial Infect Dis2008，27，1：	1
3	Characterization of Legionella pneumophila isolates from patients in Japanaccording to serogroups, monoclonaiantibody subgroups and sequencetypes显示文摘	Amemura-Maekawa J Kura F Helbig JH	2010	J MedMicrobiol2010，59，：	1
4	Sonography of nasal tip anatomy and surgical tip refinement显示文摘	TASMAN A J HELBIG M	2000	Plast Reconstr Surg2000，105，7：	1
5	Detection of Legionella pneumophila antigen in urine samples by the BinaxNOW immunochromatographic assay and comparison with both Binax Legionella Urinary Enzyme Immunoassay (EIA) and Biotest Legionella Urin Antigen EIA显示文摘	Uldum S A Luck P C	2001	J Med Microbiol2001，50，6：	1
6	The effects of short space and time scale current variability on the predictability of passive ichthyoplankton distributions: an analysis based on HF radar observations 显示文摘	Helbig J A Pepin P	2002	Fisheries Oceanography2002，11，：	1
7	Ras signaling pathway as a target for farnesyhransferase inhibitors-a new, promising prospects in the treatment for malignant disorders显示文摘	HELBIG G HOLOWIECKI J	2004	Wiad Lek2004，57，910：	1
8	Diagnosis of Echinococcus multilocularis infection by reverse-transcription polymerase chain reaction显示文摘	Kern P Frosch P Helbig M Wechsler J G Usadel S Beckh K Kunz R Lucius R Frosch M 1995	1995	Gas troenterology1995，109，2：	1
9	Variant intraneural vein-trigeminal nerve relationships: an observation during microvascular decompression surgery for trigeminal neuralgia 显示文摘	Helbig G M Callahan J D Cohen-Gadol A A	2009	Neurosurgery2009，65，5：	1
10	Pure red-cell aplasia following major and bi-directional ABO-incompatible allogeneic stem-cell transplantation:recovery of donor-derived erythropoiesis after long-term treatment using different therapeutic strategies显示文摘	Helbig G Stella-Holowieckaa B Wojnar J		0，，：	1
11	A system for automatic electrical and optical characterization of microelectromechanical devices显示文摘	HELBIG H F	1999	Microelectromech Syst1999，8，4：	1
12	T-cellabnormalities are present at high frequencies in patients with hypere-osinophilic syndrome显示文摘	Helbig G Wieczorkiewicz A Dziaczkowska-Suszek J	2009	Haematologica2009，94，9：	1
13	Phylogeny and species limits in the Palaearctic chiffchaff Phylloscopus collybita complex:mitochondrial genetic differentiation and bioacoustic evidence显示文摘	Helbig AJ Martens J Seibold I Henning F Schottler B Wink M		0，，：	1
14	Navigating the thannels and heyond: tmraw-lling Ihe genelies of Ihe epihpsies显示文摘	Helbig I Scheffer l E Mullcy J C	2008	Luncet Neurol2008，7，3：	1
15	Genetic differentiation and phylogenetic relationships of Bonelli's Warbler Phylloscopus bonelli and Green Warbler Phylloscopus nitidus显示文摘	Helbig AJ Seibold I Martens J Wink M		0，，：	1
16	A system for automatic electrical and optical characterization of microelectromechanical devices 显示文摘	Bums D J Helbig H F	1999	Journal of Microelectromechanical Systems1999，8，4：	1
17	Distribution of monoclonal antibody subgroups and sequence-based typeas amogn Legiolla pneumophila serogroup 1 isolates derived from cooling tower waterk bathwater, and soil in Japan 显示文摘	Amemura-Maekawa J Kikukawa K Helbig JH	2012	Appl Environ Microbiol2012，78，12：	1
18	The seasonal fertility of North Ameriman bison (Bison bison) bulls显示文摘	HELBIG L WOODBURY M R HAIGH J C	2007	Anim Reprod Sci2007，97，34：	1
19	Epitope mapping of immunogenic and adhesive structures in repetitive domains of- Mycoplasma boris variable surface lipoprot eins显示文摘	Sachse K Helbig J H Lysnyans ky I	2000	Infect Im- mun2000，68，2：	1
20	A System for Automatic Electrical and Optical Characterization of Microelectromechanical Devices显示文摘	Burns D J and Helbig H F	1999	Journal of Microelectromechanical System1999，8，4：	1

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