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| 1 | Crystal structures of D-psicose 3-epimerase from Clostridium cellulolyticum H10 and its complex with ketohexose sugars显示文摘D-Psicose 3-epimerase(DPEase)is demonstrated to be useful in the bioproduction of D-psicose,a rare hexose sugar,from D-fructose,found plenty in nature.Clostridium cellulolyticum H10 has recently been identified as a DPEase that can epimerize D-fructose to yield D-psicose with a much higher conversion rate when compared with the conventionally used DTEase.In this study,the crystal structure of the C.cellulolyticum DPEase was determined.The enzyme assembles into a tetramer and each subunit shows a(β/α)8 TIM barrel fold with a Mn2+metal ion in the active site.Additional crystal structures of the enzyme in complex with substrates/products(D-psicose,D-fructose,D-tagatose and D-sorbose)were also determined.From the complex structures of C.cellulolyticum DPEase with D-psicose and D-fructose,the enzyme has much more interactions with D-psicose than D-fructose by forming more hydrogen bonds between the substrate and the active site residues.Accordingly,based on these ketohexosebound complex structures,a C3-O3 proton-exchange mechanism for the conversion between D-psicose and D-fructose is proposed here.These results provide a clear idea for the deprotonation/protonation roles of E150 and E244 in catalysis. | Hsiu-Chien Chan Yueming Zhu Yumei Hu Tzu-Ping Ko Chun-Hsiang Huang Feifei Ren Chun-Chi Chen Yanhe Ma Rey-Ting Guo Yuanxia Sun | 2012 | Protein & Cell2012，3，2： | 6 |
| 2 | Inhibitory effect of different Dendrobium species on LPS-induced inflammation in macrophages via suppression of MAPK pathways显示文摘Dendrobii Caulis(DC), named ‘Shihu' in Chinese, is a precious herb in traditional Chinese medicine. It is widely used to nourish stomach, enhance body fluid production, tonify 'Yin' and reduce heat. More than thirty Dendrobium species are used as folk medicine. Some compounds from DC exhibit inhibitory effects on macrophage inflammation. In the present study, we compared the anti-inflammatory effects among eight Dendrobium species. The results provided evidences to support Dendrobium as folk medicine, which exerted its medicinal function partially by its inhibitory effects on inflammation. To investigate the anti-inflammatory effect of Dendrobium species, mouse macrophage cell line RAW264.7 was activated by lipopolysaccharide. The nitric oxide(NO) level was measured using Griess reagent while the pro-inflammatory cytokines were tested by ELISA. The protein expressions of inducible NO synthase(iNOS), cyclooxygenase-2(COX-2) and mitogen-activated protein kinases(MAPKs) phosphorylation were evaluated by Western blotting analysis. Among the eight Dendrobium species, both water extracts of D. thyrsiflorum B.S.Williams(DTW) and D. chrysotoxum Lindl(DCHW) showed most significant inhibitory effects on NO production in a concentration-dependent manner. DTW also significantly reduced TNF-α, MCP-1, and IL-6 production. Further investigations showed that DTW suppressed iNOS and COX-2 expression as well as ERK and JNK phosphorylation, suggesting that the inhibitory effects of DTW on LPS-induced macrophage inflammation was through the suppression of MAPK pathways. In conclusion, D. thyrsiflorum B.S.Williams was demonstrated to have potential to be used as alternative or adjuvant therapy for inflammation. | ZENG Qiang Chun-Hay Ko Wing-Sum Siu LI Kai-Kai Chun-Wai Wong HAN Xiao-Qiang YANG Liu Clara Bik-San Lau HU Jiang-Miao Ping-Chung Leung | 2018 | Chinese Journal of Natural Medicines2018，16，7： | 4 |
| 3 | Epidemiological shift and geographical heterogeneity in the burden of leptospirosis in China显示文摘Background:Leptospirosis morbidity and mortality rates in China have decreased since the 2000s.Further analyses of the spatiotemporal and demographic changes occurring in the last decade and its implication on estimates of disease burden are required to inform intervention strategies.In this study,we quantified the epidemiological shift and geographical heterogeneity in the burden of leptospirosis during 2005-2015 in China.Methods:We used reported leptospirosis case data from 1st January 2005 to 31st of December 2015 that routinely collected by the China Information System for Disease Control and Prevention(CISDCP)to analyze the epidemiological trend and estimate the burden in terms of disability-adjusted life-years(DALYs)over space,time,and demographical groups.Results:A total of 7763 cases were reported during 2005-2015.Of which,2403(31%)cases were the laboratoryconfirmed case.Since 2005,the notified incidence rate was gradually decreased(P<0.05)and it was relatively stable during 2011-2015(P>0.05).During 2005-2015,we estimated a total of 10313 DALYs were lost due to leptospirosis comprising a total of 1804 years-lived with disability(YLDs)and 8509 years-life lost(YLLs).Males had the highest burden of disease(7149 DALYs)compared to females(3164 DALYs).The highest burden estimate was attributed to younger individuals aged 10-19 years who lived in southern provinces of China.During 2005-2015,this age group contributed to approximately 3078 DALYs corresponding to 30%of the total DALYs lost in China.Yet,our analysis indicated a declining trend in burden estimates(P<0.001)since 2005 and remained relatively low during 2011-2015.Low burden estimates have been identified in the endemic regions where infections principally distributed.Most of the changes in DALY estimates were driven by changes in YLLs.Conclusions:In the last 11-years,the burden estimates of leptospirosis have shown a declining trend across the country;however,leptospirosis should not be neglected as it remains an important zoonotic disease and potentially affecting the young and productive population in economically less-developed provinces in southern of China.In addition,while in the last five years the incidence has been reported at very low-level,this might not reflect the true incidence of leptospirosis.Strengthened surveillance in the endemic regions is,hence,substantially required to capture the actual prevalence to better control leptospirosis in China. | Pandji Wibawa Dhewantara Abdullah AMamun Wen-Yi Zhang Wen-Wu Yin Fan Ding Danhuai Guo Wenbiao Hu Federico Costa Albert Icksang Ko Ricardo JSoares Magalhaes | 2018 | Infectious Diseases of Poverty2018，7，1： | 2 |
| 4 | Arbuscular mycorrhizal fungi increase organic carbon decomposition under elevated CO2显示文摘 | Cheng L Booker FL Tu C Burkey KO Zhou LS Shew HD Rufty TW Hu SJ | | 0，，： | 1 |
| 5 | Hypolipidemic effect of glycosaminoglycans from the cucumber Metriatyla scabra in rats fed a cholesterol-sipplemented diet 显示文摘 | Liu His Hsien Ko Wen Ching Hu Miao Lin | 2002 | J Agrie Food Chem2002，50，12： | 1 |
| 6 | Patterns of retinal ganglion cell survival after brain-derived neurotrophic factor administration in hypertensive eyes of rats显示文摘 | Ko ML Hu DN Ritch R | 2001 | Neurosci Lett2001，305，2： | 1 |
| 7 | The effect of fineness on the properties of the blended cements incorporating ground granulated blast furnace slag and ground basaltic pumice显示文摘 | Hanifi Binici Hu seyin Temiz Mehmet M Ko se | 2007 | Construction and Building Materials2007，21，5： | 1 |
| 8 | Raman spectroscopicstudy of effect of steam and carbon dioxide activation onmicrostructure of polyacrylonitrile-based activated car-bon fabrics显示文摘 | KO T H KUO W S HU C H | 2001 | Journal of Applied Polymer Science2001，81，1： | 1 |
| 9 | A physics-based MOSFET noise model for circuit simulators 显示文摘 | HUNG K K KO P K HU C | 1990 | IEEE Trans on ED1990，37，5： | 1 |
| 10 | Peptidergic intra- epidermal nerve fibers in the skin contribute to the neuropathic pain in paclitaxel-induced periph- eral neuropathy 显示文摘 | Ko MH Hu ME Hsieh YL | 2014 | Neuropeptides2014，48，3： | 1 |
| 11 | Bending properties of multiaxial warp knitted fabrics显示文摘 | Ko Frank Hu Jin-lian Jiang Ya-ming | 1998 | Textile Asia1998，29，2： | 1 |
| 12 | Change of microstructure and characteristics of carbon/carbon composites when mesophase was added during graphitization 显示文摘 | Hu H L Ko T H Kuo W S | 2005 | J Appl Polym Sci2005，98，： | 1 |
| 13 | Modeling uniaxial tensile properties of multiaxial warp knitted fabrics 显示文摘 | HU J L JIANG Y M KO F | 1998 | Text Res J1998，68，11： | 1 |
| 14 | Patterns of retinal ganglion cell survival after brain-derived neurotrophic factor administration in hypertensive eyes of rats显示文摘 | Mei-Lan Ko Dan-Ning Hu Robert Ritch S.C Sharma Chau-Fong Chen | 2001 | Neuroscience Letters2001，，2： | 1 |
| 15 | Dynamic thresholdvoltage MOSFET ( DTMOS ) for ultra-low voltage VLSI 显示文摘 | ASSADERAGHI F HU C M KO P K | 1997 | IEEE Trans on ED1997，44，3： | 1 |
| 16 | Hypolipidemic effect of glycosamin- oglycans from the cucumber Metriatyla scabra in rats fed a choles- terol-supple menteddiet显示文摘 | Liu H H Ko W C Hu M L | 2002 | J Agric Food Chem2002，50，12： | 1 |
| 17 | Bending properties of multiaxial warp knitted fabrics 显示文摘 | KO F HU J L JIANG Y M | 1998 | Textile Asia1998，29，2： | 1 |
| 18 | Observation of velocity over- shoot in silicon inversion layers 显示文摘 | ASSADERAGHI F KO P K HU C | 1993 | IEEE Electron Device Lett1993，14，10： | 1 |
| 19 | A web-based virtual laboratory on a frequency modulation experiment显示文摘 | Ko C C Chen B M Hu S Y | 2001 | IEEE Transactionson Systems Man and Cybernetics Part C:Applications and Reviews2001，31，3： | 1 |
| 20 | A web based virtual laboratory on a frequency modulation experiment显示文摘 | Ko C C Chen B M Hu S Y el al | 2001 | IEEE Transactions on Systems Man and Cybernetics Part C:Applications and Reviews2001，31，3： | 1 |
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