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| 1 | 楚都纪南城的勘查与发掘(上)显示文摘前言湖北省江陵县境内的楚都纪南城,是迄今已发现的我国南方最大的一座古城。楚都纪南城即楚郢都故城,因在纪山之南,后人称之为纪南城。它在今荆州城(即江陵县城)北5公里(图一)。城址周围土地肥沃,水陆交通便利。东接富饶的江汉平原,西临险要的鄂西山地,南有浩瀚的万里长江,北有大道与中原相通。城址附近地势基本平坦,只有一些起伏不大的土岗丘陵。 | Hubei Provincial Museum | 1982 | 考古学报1982，，3： | 84 |
| 2 | 江陵天星观1号楚墓显示文摘天星观1号墓位于江陵县观音垱公社五山大队境内,东临长湖,西距楚故都纪南城约30公里(图一)。清时曾在该墓封土堆上修建过'天星观'道观一座,因此而得名。五山大队境内自东向西弧形排列五个大土塚,1号墓位于'五山'东侧,是'五山'之中最大的一个,海拔40.4米(图版拾壹,1)。 | The Jingzhou District Museum, Hubei Province | 1982 | 考古学报1982，，1： | 168 |
| 3 | 楚都纪南城的勘查与发掘(下)显示文摘肆松柏区30号建筑遗址的发掘松柏区30号建筑遗址在纪南城内东南部,南距南城垣约1300、东距东城垣约1400米。夯土台基暴露在地面上,高1.5—2、长70、宽50米。台基南15米处,有汉代夯筑土堤,今俗称'火龙堤'。 | Hubei Provincial Museum | 1982 | 考古学报1982，，4： | 62 |
| 4 | Analysis of in vivo patterns of caspase 3 gene expression in primary hepatocellular carcinoma and its relationship to p21^(WAF1) expression and hepatic apoptosis显示文摘AIM To detect the expression of caspase 3gene in primary human hepatocellular carcinoma(HCC)and investigate its relationship to p21WAF1gene expression and HCC apoptosis.METHODS In situ hybridization was employedto determine caspase 3 and p21WAF1expression inHCC.In situ end-labeling was used to detecthepatocytic apoptosis in HCC.RESULTS Twenty-one of 39(53.8%)cases ofHCC were found to express caspase 3transcripts,while 45.2% of HCC failed toexpress caspase 3.Non-cancerous adjacent livertissues showed more positive caspase 3(87.5%,7/8)as compared with HCC(P<0.05).The expression of caspase 3 is correlated withHCC differentiation,72.2%(13/18)ofmoderately to highly differentiated HCC showedcaspase 3 transcripts positive,while only 38.1%of poorly differentiated HCC harbored caspase 3transcripts(P<0.05).No relationship wasfound between caspase 3 expression and tumorsize or grade or metastasis,although 52.5%(5/8)of HCC with metastasis were caspase 3positive and a little higher than that with nometastasis(51.6%,P>0.05).Expression of caspase 3 alone did not affect the apoptosisindex(AI)of HCC.The AI was 7.12%o in caspase3-positive tumors(n=21),while in caspase 3-negative cases(n=18)6.59%0(P>0.05).Expression of caspase 3 clearly segregated withp21WAF1positive tumors as compared withp21WAF1-negative cases(16 of 23,69.6% versus5 of 16,31.3%)with statistical significance(P=0.017).In the cases with positive caspase 3and negative p21WAF1,the Al was found slightlyhigher,but with no statistical significance,thanthat with expression of p21WAF1and caspase 3(7.21‰ vs 6.98‰,P>0.05).CONCLUSION Loss of caspase 3 expressionmay contribute to HCC carcinogenesis,althoughthe expression of caspase 3 does not correlatewell with cell apoptosis in HCC.p21WAF1may bemerely one of the inhibitors which can reducecaspase 3 mediated cell apoptosis in HCCs. | Bao Hua Sun Jun Zhang Bao JǜWang Xi Ping Zhao You Kun Wang Zhi Qun Yu Dong Liang Yang Lian Jie Hao Department of Clinical Immunology,Tongji Hospital,Tongji Medical University,Wuhan 430030,Hubei Province,China | 2000 | World Journal of Gastroenterology2000，6，3： | 65 |
| 5 | 宜昌中堡岛新石器时代遗址显示文摘中堡岛(又名松苞岛、中包岛)位于长江西陵峡境三斗坪镇(因建葛洲坝该镇已拆迁)西1公里许,四周为砂石堆积的河漫滩,中部隆起而平缓,其上竹树葱笼,远视如一座堡垒,故名。全岛东西长约1000、南北宽300—400米,西端隔茅坪溪与秭归县茅坪镇相望,南侧近山,中隔一条宽约数十米的江汊;北临长江(图一;图版壹,1)。 | The Yichang Prefectural Museum, Hubei Province, and the Department of History, Sichuan University | 1987 | 考古学报1987，，1： | 55 |
| 6 | Effect of Nimesulide on proliferation and apoptosis of human hepatoma SMMC-7721 cells显示文摘AIM: Cyclooxygenase-2 (COX-2) has been suggested to beassociated with carcinogenesis. We sought to investigatethe effect of the selective COX-2 inhibitor, Nimesulide onproliferation and apoptosis of SMMC-7721 human hepatomacells.METHODS: This study was carried out on the culture ofhepatic carcinoma SMMC-7721 cell line. Variousconcentrations of Nimesulide (0、200 μmol/L、300 μmol/L、400μmol/L) were added and incubated. Cell proliferation wasdetected with MTT colorimetric assay, cell apoptosis byelectron microscopy, flow cytometry and TUNEL.RESULTS: Nimesulide could significantly inhibit SMMC-7721cells proliferation dose-dependent and in a dependentmanner compared with that of the control group. Theduration lowest inhibition rate produced by Nimesulide inSMMC-7721 cells was 19.06 %, the highest inhibition ratewas 58.49 %. After incubation with Nimesulide for 72 h, themost highest apoptosis rate and apoptosis index of SMMC-7721 cells comparing with those of the control were 21. 20 %+1.62% vs2.24% +0.26% and21.23+ 1.78 vs2.01+0.23(p<0.05).CONCLUSION: The selective COX-2 inhibitor, Nimesulide caninhibit the proliferation of SMMC-7721 cells and increaseapoptssis rate and apoptosis index of SMMC-7721 cells. Theapoptoois rate and the apoptosis index are dose-dependent.Under electron microscope SMMC-7721 cells incubated with 300μmol and 400 μmol Nimesulide show apoptotic characteristics.With the clarification of the mechanism of selective COX-2inhibitors, These COX-2 selective inhibitors can become thechoice of prevention and treatment of cancers. | Geng Tian Jie-Ping Yu He-Sheng Luo Bao-Ping Yu Hui Yue Jian-Ying Li Oiao Mei,Gastroenterology department,Renmin hospital of Wuhan university,Wuhan 430060,Hubei Province,China | 2002 | World Journal of Gastroenterology2002，8，3： | 51 |
| 7 | Oscillation of Solutions of Nonlinear Partial Differential Equations of Neutral Type | Yu Yuanhong Liu Bin Liu Zhengrong Institute of Applied Mathematics, Academia Sinica, Beijing 100080, China Department of Mathematics, Hubei Normal College, Huangshi 435002. China Department of Mathematics, Yunnan University. Kunming 650091. China Institute of Applied Mathematics of Yunnan Province, Kunming 650091, China | 1997 | Acta Mathematica Sinica,English Series1997，13，4： | 39 |
| 8 | The immunotherapeutic effect of dendritic cells vaccine modified with interleukin-18 gene and tumor cell lysate on mice with pancreatic carcinoma显示文摘AIM:To estimate the effect of a therapeutic vaccine against pancreatic carcinoma based on dendritic cell(DC)vaccine modified with tumor lysate and Interleukin-18 gene.METHODS:The BALB/C mice model of pancreatic arcinoma was induced with DMBA,DCvaccine was construted through pulsed with tumor lysate and transfected by the recombinant adenoviral vector encoding IL-18gene.The immnotherapeutic effects of DC vaccine on mice with pancreatic carcinoma were assessed (divided intoD-IL18-Lysate group,DC-Lysate group,DC-IL18 group,DC group,PBSgroup).RESULTS:After vaccination of the DC vaccine,the concentration of IL-18and IFN-γwere 2161±439ng·L^-1and435±72ng·L^-1inDC-IL18-Lysate group and there was significant difference compared with other groups(P<0.01)After vaccination of the DCcvaccine,the transplanted tumors were observed on 30days inDC-Lysate groups.on 16daysinDC-IL18groups.on3days in control group,but mice remained tumor-free for at least50days in DC-IL18-Lysate group and there was significant difference between DC-IL18-Lysate group and other groups(P<0.01).The median survival exceeds62days in DC-IL18-Lysate group.But the medsian surival was 48.6days in DC-Lysate group,33days in DC-IL-18group,17days in PBSgroup.The survival period was obviously prolonged in DC-IL18-Lysate group than in other groups(P<0.05,P<0.01).The weight of pancreatic tumor was0.22±0.083ginDC-IL18-Lysate group,1.45±0.74ginDC-Lysate group,1.89±1.34gin DC-IL18group,3.0±1.6ginDCgroup,2.9±2.0ginPBSgroup and the weight of tumor obviously reduced in DC-IL-18-Lysate group than in other groups(P<0.05,P<0.01).CONCLUSION:DC vaccine modified with tumo lysate and Interleukin-18gene can induce a specific and effective immune response against pancreatic cancinoma,cell. | Zhao-Hui Tang Gao-Song Wu Sheng-Quan Zou Fa-Zhu Qiu Department of Surgery of Tong Ji Hospital Wen-Hong Qiu Department of immunology,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,Hubei Province,China Xiang-Ping Yang Department of Biochemistry,Rheinisch-Westfalische Technische Hochschule(RWTH),D-52074 Aachen,Germany | 2002 | World Journal of Gastroenterology2002，8，5： | 37 |
| 9 | The influence of Enteral Nutrition in postoperative patients with poor liver function显示文摘AIM: To investigate the safety, rationality and the practicality of enteral nutritional (EN) support in the postoperative patients with damaged liver function and the protective effect of EN on the gut barrier.METHODS: 135 patients with liver function of Child B or C grade were randomly allocated to enteral nutrition group (EN, 65 cases), total parenteral nutrition group (TPN, 40cases) and control group (CON, 30 cases). Nutritional parameters, hepatic and kidney function indexes were measured at the day before operation, 5th and 10th day after the operation respectively. Comparison was made to evaluate the efficacy of different nutritional support. Urinary concentrations of lactulose(L) and mannitol(M) were measured by pulsed electrochemical detection(HPLC-PED)and the L/M ratio calculated to evaluate their effectiveness on protection of gut barrier.RESULTS: No significant damages in hepatic and kidney function were observed in both EN and TPN groups between pre- and postoperatively. EN group was the earliest one reaching the positive nitrogen balance after operation and with the lowest loss of body weight and there was no change in L/M ratio after the operation (0.026±0.004) at the day 1before operation, 0.030±0.004 at the day 5 postoperative and 0.027±0.005 at the day 10 postoperative), but the change in TPN group was significant at the day 5postoperative (0.027±0.003 vs 0.038±0.009,P<0.01).CONCLUSION: EN is a rational and effective method in patients with hepatic dysfunction after operation and has significant protection effect on the gut barrier. | Qing-Gang Hu Qi-Chang Zheng Department of Surgery,Xiehe hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,China | 2003 | World Journal of Gastroenterology2003，9，4： | 38 |
| 10 | Helicobacter pylori infection generated gastric cancer through p53-Rb tumor-suppressor system mutation and telomerase reactivation显示文摘AIM: To investigate the relationship between Helicobacterpylori (H. pylori) infection and the expressions of the p53,Rb, c-myc, bcl-2 and hTERT mRNA in a series of diseasesfrom chronic gastritis (CG), intestinal metaplasia type Ⅰ or Ⅱ(IMⅠ-Ⅱ), intestinal metaplasia type Ⅲ (IMⅢ), mild or modestdysplasia (DysⅠ-Ⅱ), severe dysplasia (DysⅢ) to gastric cancer(GC) and to elucidate the mechanism of gastriccarcinogenesis relating to H.pyloriinfection.METHODS: 272 cases between 1998 and 2001 wereavailable for the study including 42 cases of CG, 46 cases ofIMⅠ-Ⅱ, 25 cases of IMⅢ, 48 cases of DysⅠ-Ⅱ, 27 cases ofDysⅢ, 84 cases of GC.-H. pyloriinfection and the expressionsof p53, Rb, c-myc, bcl-2 were detected by means ofstreptavidin-peroxidase (SP) immunohistochemical method.HTERT mRNA was detected byin situ hybridization(ISH).RESULTS: The expressions of p53, Rb, c-myc, hTERT mRNAand bcl-2 were higher in the GC than in CG, IN, Dys. Theexpression of c-myc was higher in IMⅢ with-H.pyloriinfection(10/16) than that without infection (1/9) and the positive ratein DysⅠ-Ⅱ and DysⅢ with-H.pyloriinfection was 18/30 and 13/17, respectively, higher than that without infection (4/18 and3/10, respectively). In our experiment mutated p53 had noassociation with H.pyloriinfection, theexpression of Rb wasassociated with-H. pyloriinfection in GC, but the p53-Rb tumor-suppressor system abnormal in DysⅠ-Ⅱ cases, DysⅢⅡ and GCcases with H. pyloriinfection was 21/30, 15/17 and 48/48respecively, higher than non-infection groups (4/18, 3/10, 28/36). Furthermore the level of hTERT mRNA in GC with H. pyloriinfection (47/48) was higher than that without infection (30/36), however the relationship between bcl-2 and H. pyloriwasonly in IMⅢ. C-myc had a close association with hTERT mRNAin DysⅢ and GC (P=0.0 253,0.0 305 respectively).CONCLUSION: In the gastric carcinogenesis, H. pylorimightcause the severe imbalance of proliferation and apoptosisin the precancerous lesions (IMⅢ and GysⅢ) first, leadingto p53-Rb tumor-suppressor system mutation and telomerasereactivation, and finally causes gastric cancer. | Jing Lan Yong-Yan Xiong Yi-Xian Lin Bi-Cheng Wang Ling-Ling Gong Hui-Sen Xu Guang-Song Guo Department of Pathology,Zhongnan Hospital,Wuhan University,Wuhan city 430071,Hubei Province,China | 2003 | World Journal of Gastroenterology2003，9，1： | 39 |
| 11 | Assessment of candidate plant DNA barcodes using the Rutaceae family显示文摘DNA barcoding is a rapidly developing frontier technology that is gaining worldwide attention.Here,seven regions (psbA-trnH,matK,ycf5,rpoC1,rbcL,ITS2,and ITS) with potential for use as DNA barcodes were tested for their ability to identify 300 samples of 192 species from 72 genera of the family Rutaceae.To evaluate each barcode’s utility for species authentication,PCR amplification efficiency,genetic divergence,and barcoding gaps were assessed.We found that the ITS2 region exhibited the highest inter-specific divergence,and that this was significantly higher than the intra-specific variation in the 'DNA barcoding gap' assessment and Wilcoxon two-sample tests.The ITS2 locus had the highest identification efficiency among all tested regions.In a previous study,we found that ITS2 was able to discriminate a wide range of plant taxa,and here we confirmed that ITS2 was also able to discriminate a number of closely related species.Therefore,we propose that ITS2 is a promising candidate barcode for plant species identification. | LUO Kun1,2,CHEN ShiLin1,CHEN KeLi2,SONG JingYuan1,YAO Hui1,MA XinYe1,ZHU YingJie3,PANG XiaoHui1,YU Hua1,LI XiWen1,4 & LIU Zhen2 1Institute of Medicinal Plant Development,Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100193,China 2College of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430061,China 3School of Bioscience and Engineering,Southwest Jiaotong University,Chengdu 610031,China 4Department of Chemistry,Tsinghua University,Beijing 100084,China | 2010 | Science China(Life Sciences)2010，53，6： | 32 |
| 12 | Effects of hydroxyapatite nanoparticles on proliferation and apoptosis of human hepatoma BEL-7402 cells显示文摘AIM: To study the effect of hydroxyapatite (HAP) nanoparticleson human hepatoma cell line BEL-7402 in vitro.METHODS: The human hepatoma cell line BEL-7402 wascultured and treated with HAP nanoparticles at variousconcentrations. Growth suppression was detected with MTTcolorimetric assay, cell apoptotic alterations were evaluatedby cytochemical staining (Hoechst 33258), transmissionelectron microscopy (TEM), and flow cytometry (FCM).RESULTS: HAP nanoparticles inhibited the growth ofhepatoma cells in a dose-dependent manner, with IC50 valuesof 29.30 mg/L. Treated with 50-200 mg/L HAP nanoparticlesfor 48 h, BEL-7402 cells apoptosis with nuclear chromatincondensation and fragmentation as well as cell shrinkageand the formation of apoptotic bodies were observed undercytochemical staining and transmission electron microscopy.FCM analysis showed hypodiploid peaks on histogram, theapoptotic rates at the concentrations of 50, 75, 100, 150and 200 mg/L of HAP nanoparticles were 20.35±2.23%,25.35±1.92%, 29.34±4.61%, 44.92±3.78 % and53.64±3.49%, respectively, which were all significantlyhigher than that of control group 2.23±0.14%. There wasa significant correlation between HAP nanoparticleconcentration and apoptotic rate (r=0.994, P<0.01).CONCLUSION: HAP nanoparticles not only inhibitproliferation but also induce apoptosis of human hepatoma cell line BEL-7402 in vitro. | Zhi-Su Liu Sheng-Li Tang Zhong-Li Ai Department of General Surgery,Zhongnan Hospital of Wuhan University,Wuhan 430071,Hubei Province,China | 2003 | World Journal of Gastroenterology2003，9，9： | 33 |
| 13 | Proliferative activity of bile from congenital choledochal cyst patients显示文摘AIM: To explore the potential carcinogenicity of bile from congenital choledochal cyst (CCC) patients and the mechanism of the carcinogenesis in congenital choledochal cyst patients.METHODS: 20 bile samples from congenital choledochalcyst patients and 10 normal control bile samples were usedfor this study. The proliferative effect of bile was measuredby using Methabenzthiazuron (MTT) assay; Cell cycle andapoptosis were analyzed by using flow cytometry (FCM),and the PGE2 levels in the supernatant of culturedcholangiocarcinoma cells were quantitated by enzyme-linkedimmunoabsordent assay (ELISA).RESULTS: CCC bile could significantly promote theproliferation of human cholangiocarcinoma QBC939 cellscompared with normal bile (P=0.001) and negative controlgroup (P=0.002), and the proliferative effect of CCC bilecould be abolished by addition of cyclooxygenase-2 specificinhibitor celecoxib (20 μM). The QBC939 cells proliferativeindex was increased significantly after treated with 1% bilefrom CCC patient (P=0.008) for 24 h, the percentage of Sphase (29.48±3.27)% was increased remarkably (P<0.001)compared with normal bile (11.72±2.70) %, and thepercentage of G0/G1 phase (54.19±9.46) % was decreasedremarkably (P=0.042) compared with normal bile (69.16±10.88) %, however, bile from CCC patient had no significantinfluence on apoptosis of QBC939 cells (P=0.719).CONCLUSION: Bile from congenital choledochal cyst patientscan promote the proliferation of human cholangiocarcinomaQBC939 cells via COX-2 and PGE2 pathway. | Gao-Song Wu Sheng-Quan Zou Xian-Wen Luo Jian-Hong Wu Zheng-Ren Liu Department of General Surgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430030,Hubei Province,China | 2003 | World Journal of Gastroenterology2003，9，1： | 30 |
| 14 | Transforming growth factor-β1 in invasion and metastasis in colorectal cancer显示文摘AIM:To investigate the role of TGFβ1 in invasion and metastasis in colorectal cancer by analysing TGFβ1 correlated wity depth of tumor invasion,stage and metastasis.METHODS:Serum TGFβ1levels were determined in50patients with colorectal cancer and 30healthy volunteers using a TGFβ1 enzyme-linked immunosorbent assay.TGFβ1 expression in primary and lymph node metastatic lesions were detected in 98cases of colorectal cancer by immunohistochemical staining and in situ hybridization.RESULTS:Serum levels of TGFβ1 in patients with colorectal cancer(40±18μg·L^-1)were significantly higher than those in the healthy control group(19±8μg·L^-1),P<0.05.Elevated levels of serum TGFβ1were found in 60%of patients with colorectal cancer when the mean+2s was used as the upper limit of the normal range(35.1μg·L^-1).Increases in serum TGFβ1 levels were significantly asociated with Dukei's stage(P<0.05),but there was no significant difference between,Duki's stage Bpatients and Dukei's stage Cpatients.In the cytoplasm of cancer cells,TGFβ1 was immunostained in37.8%(37/98)of colorectal cancer,and this expression was confirmed by in situ hybridization,Among35cases of colorectal cancer with lymph node metastatic lesions,TGFβ1 positive staining was found in18(51.4%)cases of primary tumor,and 25(71.4%)cases with lymph node metastatic lesions,respectively,Of17cases with no staining in the primary lesion.7(41.2%)casesshowed TGFβ1 staining in the metastatic lesion.Serum TGFβ1 levels and TGFβ1 expression in colorectal cancer tissues were correlated significantly with depth of tumor invasion,stage and metastasis,Patients in stage C-D,T3-T4and with metastasis had significantly higher TGFβ1 levels than patients in stage A-B,T1-T2and without metastasis(P<0.05).CONCLUSION:These results suggest that transforming growth factor-β1 is closely related to the invasion and metastasis of colorectal cancer.It increased the invasive and metastatic potential of tumor by altering a tumor microenvironment.TGFβ1 may be used as a possible biomarke. | Bin Xiong Hong-Yin Yuan Ming-Bo Hu Feng Zhang Zheng-Zhuan Wei Ling-Ling Gong Guo-Liang Yang Department of Oncology,Affiliated Zhongnan Hospital of Wuhan University,Wuhan 430071,Hubei Province,China | 2002 | World Journal of Gastroenterology2002，8，4： | 25 |
| 15 | Celecoxib inhibits proliferation and induces apoptosis via prostaglandin E_2 pathway in human cholangiocarcinoma cell lines显示文摘AIM: To evaluate the roles and mechanisms of celecoxib in inducing proliferation inhibition and apoptosis of human cholangiocarcinoma cell lines.
METHODS: Cyclooxygenase-2-overexpressing human cholangiocarcinoma cell line QBC939 and cyclooxygenase2-deficient human cholangiocarcinoma cell line SK-CHA-1were used in the present study. The anti-proliferative effect was measured by methabenzthiazuron (MTT) assay;apoptosis was determined by transferase-mediated dUTP nick end labeling (TUNEL) detection and transmission electron microscopy (TEN). Cell cycle was analyzed by flow cytometry (FCM). The PGE2 levels in the supernatant of cultured cholangiocarcinoma cells were quantitated by enzyme-linked immunoabsordent assay (ELISA).
RESULTS: Celecoxib suppressed the production of PGE2and inhibited the growth of QBC939 cells. Celecoxib at 10,20, and 40 μmol/L inhibited PGE2 production by 26 %,58 %, and 74 % in QBC939 cells. The PGE2 level was much lower constitutively in SK-CHA-1 cells (18.6±3.2)compared with that in QBC939 (121.9±5.6) cells (P<0.01)and celecoxib had no significant influence on PGE2 level in the SK-CHA-1 cells. The PGE2 concentration in SK-CHA-1cells also reduced but not significantly after treatment with celecoxib. The PGE2 concentration in SK-CHA-1 cells was (16.5±2.9) ng/well, (14.8±3.4) ng/well, (13.2±2.0) ng/well and (12.6±3.1) ng/well respectively, when pre-treated with 1 Jmol/L, 10 Jmol/L, 20 Jmol/L and 40 Jmol/L of celecoxib for 48 h (P>0.05, vs control). The anti-proliferation effect of celecoxib (20 Jmol/L) on QBC939 cells was time-dependent,it was noticeable on day 2 (OD490=0.23±0.04) and became obvious on day 3 (OD490=0.31±0.07) to day 4 (OD490=0.25±0.06), and the OD490 in the control group (day 1)was 0.12±0.03 (P<0.01, vscontrol). The anti-proliferation effect of celecoxib could be abolished by the addition of 200 pg/mL PGE2. The proliferation of SK-CHA-1 cells was inhibited slightly by celecoxib, the cell density OD490 in the presence of celecoxib and in control group was 0.31±0.04 and 0.42±0.03 respectively on day 2 (P>0.05), 0.58±0.07 and 0.67±0.09 respectively on day 3 (P>0.05), and 0.71±0.08 and 0.78±0.06 respectively on day 4 (P>0.05). Celecoxib induced proliferation inhibition and apoptosis by G1-S cell cycle arrest: the percentage of QBC939 cells in G0-G1 phase after treatment with 40 Jmol/L (74.66±6.21) and 20 Jmol/L (68.63±4.36) celecoxib increased significantly compared with control cells (54.41±5.12, P<0.01). The percentage of SK-CHA-1 cells in G0-G1 phase after treatment with various concentrations of celecoxib didn't change significantly compared with control cells. The TUNEL index was much higher in QBC939 cells treated with 20 Jmol/L celecoxib for 2 d (0.063±0.018) and for 4 d (0.102±0.037) compared with control cells (0.017±0.004, P<0.01).
CONCLUSION: The currentin vitro study indicates that inhibition of proliferation and induction of apoptosis in human cholangiocarcinoma cells by cyclooxygenase-2 specific inhibitor celecoxib may involve in COX-dependent mechanisms and PGE2 pathway. Celecoxib as a chemopreventive and chemotherapeutic agent might be effective primarily on COX2-expressing cholangiocarcinoma. | Gao-Song Wu Sheng-Quan Zou Zheng-Ren Liu Zhao-Hui Tang Ju-Hua Wang Department of General Surgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430030,Hubei Province,China | 2003 | World Journal of Gastroenterology2003，9，6： | 26 |
| 16 | Volcanism at the Permian-Triassic Boundary in South China and Its Effects on Mass Extinction显示文摘This paper discusses the clayrocks widespread at the Permian-Triassic boundary, which are mostly of volcanic origin. Volcanogenetic textures, structures and minerals such as high-temperature quartz are found in clayrocks at the Permian-Triassic boundary in many places. Thousands of microspherules have been collected from the Boundary clayrocks, many of which exhibit the typical features of the process from melting to cooling and solidification. indicating that they were formed by volcanic eruption or extraterrestrial impact. Volcanic effects on the Permian-Triassic mass extinction may be reflected in conodonts, algae and ammonoids. The Boundary clayrocks are found in many Permian-Triassic sections along the coast of Tethys. Their orighin remains to be studied. | Yin Hongfu, Huang Siji, Zhang Kexin, Yang Fengqing, Ding Meihua, Bi Xianmei and Zhang Suxin China University of Geosciences, Wuhan, Hubei | 1989 | Acta Geologica Sinica(English Edition)1989，63，4： | 22 |
| 17 | Expression and signifi cance of TLR4 and HIF-1α in pancreatic ductal adenocarcinoma显示文摘AIM:To investigate the expression of toll-like receptor(TLR) 4,nuclear factor-κB(NF-κB) p65 and hypoxiainducible transcription factor 1α(HIF-1α) in pancreatic ductal adenocarcinoma and their clinical significance.METHODS:The mRNA of TLR4 and HIF-1α were investigated by real-time polymerase chain reaction in 30 cases of pancreatic ductal adenocarcinoma and its adjacent tissues,and expression of TLR4,NF-κB p65 and HIF-1α protein were detected by immunohistochemistry in 65 cases of pancreatic ductal adenocarcinoma tissues and 38 cases of corresponding adjacent tissues.The relationship between TLR4 or HIF-1α and pathologic features,as well as the association between TLR4 and HIF-1α,were also analyzed.Kaplan-Meier method was used to assess the impact of expression of TLR4 and HIF-1α on survival of patients with pancreatic cancer.RESULTS:The relative quantif ication of TLR4 and HIF-1α mRNA in tumor tissues was 0.81±0.10 and 0.87±0.11,respectively,signif icantly higher than that in adjacent tissues(0.81±0.10 vs 0.70±0.16,P=0.002;0.87±0.11 vs 0.68±0.13,P=0.000).The protein expression of TLR4,NF-κB p65 and HIF-1α in tumor tissues was 69.20%,66.15% and 70.80%,respectively,being signif icantly higher than that in adjacent normal tissues(69.20% vs 39.50%,P=0.003;66.15% vs 31.58%,P=0.001;70.80% vs 36.80%,P=0.001).There was no signif icant correlation between TLR4 or HIF-1α expression and the age,gender,tumor location,the degree of tumor differentiation in the patients(P>0.05).However,there was signif icant correlation between the expression of TLR4 or HIF-1α and tumor size,lymph node metastasis,venous invasion and clinical staging(P<0.05).The expression of TLR4 and HIF-1α had a signif icant impact on survival of patients with pancreatic adenocarcinoma.CONCLUSION:TLR4,NF-κB p65 and HIF-1α are overexpressed in pancreatic adenocarcinoma,TLR4 may be partly involved in up-regulating HIF-1α,and both synergestically promote development of pancreatic adenocarcinoma. | Jian-Jun Zhang,He-Shui Wu,Lin Wang,Yuan Tian,Jing-Hui Zhang,Hai-Long Wu Department of Pancreatic Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,China Department of Pediatrics,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,China Laboratory of General Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,China | 2010 | World Journal of Gastroenterology2010，16，23： | 21 |
| 18 | Role of VEGF and CD44v6 in differentiating benign from malignant ascites显示文摘AIM: To detect the vascular endothelial growth factor (VEGF)and soluble splice variant 6 of CD44 (sCD44v6) levels in ascites and to explore their role in differentiating benign from malignant ascites.METHODS: Cirrhotic ascites (n=36), tuberculosis ascites (n=8) and malignant ascites (n=23) were collected and studied. Concentrations of soluble VEGF and sCD44v6 in various kinds of ascites (n=67) were measured using a sandwich enzyme-linked immunoadsorbent assay.RESULTS: VEGF and sCD44v6 levels in malignant ascites were 640.74±264.81 pg/ml and 89.22±38.20 ng/ml,respectively, both of which were significantly higher than those in cirrhotic ascites and tuberculous ascites (q=18.98,11.89 and q=8.92, 5.09; P<0.01). However, the levels of VEGF and sCD44v6 in cirrhotic and tuberculous ascites had no significant difference (q=0.48, 0.75; P>0.05).Furthermore, VEGF levels in malignant ascites in patients with ovarian cancer were higher than those with gastric and colon cancer (q=5.03, 6.79; P<0.01, respectively). But differences of VEGF levels between gastric and colon cancer were not significant (q=1.90, P>0.05). Whereas, sCD44v6 levels in malignant ascites from patients with ovadan, gastric and colon cancer had no significant difference (q=0.06, 0.91,0.35;P>0.05, respectirely). In comparison with cirrhotic and tuberculous ascites, when the upper limit of its VEGF mean levels 119.44 pg/ml (70.90±48.54) and sCD44v6 mean levels 63.59 ng/ml (44.42±19.17) was taken as the minimum cutoff limit, the sensitivity and specificity of VEGF and sCD44v6 of this assay to the diagnos is of malignant ascites were 91.3 %, 90.9 % and 73.9 %, 88.7 % respectively.CONCLUSION: Elevated levels of VEGF and sCD44v6 may be useful in differential diagnosis of benign and malignant ascites. | Wei-Guo Dong Xiao-Min Sun Bao-Ping Yu He-Sheng Luo Jie-Ping Yu, Department of Gastroenterology, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China | 2003 | World Journal of Gastroenterology2003，9，11： | 21 |
| 19 | An implantable rat liver tumor model for experimental transarterial chemoembolization therapy and its imaging features显示文摘AIM: To establish an ideal implantable rat liver tumor model for interventional therapy study and examine its angiographic signs and MRI, CT features before and after embolization. METHODS: Forty male Wistar rats were implanted with Walker256 tumor in the left lateral lobe of liver. Digital subtraction angiography (DSA) and transarterial chemoembolization were performed on day 14 after implantation. Native computer tomography (CT, n=8) and native magnetic resonance (MR,n=40) were performed between the day 8 and day 21 after implantation. The radiological morphological characteristics were correlated with histological findings.RESULTS: Successful implantation was achieved in all forty rats, which was confirmed by CT and MRI. MR allowed tumor visualization from day 8 while CT from day 11 after implantation. The tumors were hypodensity on CT, hypointense on MR T1-weighted and hyperintense on T2-weighted. The model closely resembled human hepatocardnoma in growth pattem and the lesions were rich in vasculature on angiography and got its filling mainly from the hepatic artery. Before therapy, tumor size was 211.9±48.7 mm3. No ascites, satellite liver nodules or lung metastasis were found. One week after therapy, tumor size was 963.6±214.8 mm3 in the control group and 356.5±78.4mm3 in TACE group. Ascites (4/40), satellite liver nodules (7/40) or lung metastasis (3/40) could be seen on day 21.CONCLUSION: Walker-256 tumor rat model is suitable for the interventional experiment. CT and MRI are helpful in animal optioning and evaluating experimental results. | Xin Li Chuan-Sheng Zheng Gan-Sheng Feng Chen-Kai Zhuo Jun-Gong Zhao Xi Liu,Department of Interventional Radiology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,China | 2002 | World Journal of Gastroenterology2002，8，6： | 20 |
| 20 | Influence of transarterial chemoembolization on angiogenesis and expression of vascular endothelial growth factor and basic fibroblast growth factor in rat with Walker-256 transplanted hepatoma:An experimental study显示文摘AIM: After transarterial chemoembolization (TACE), the residual cancer cells are under extensive hypoxic or even anoxic environment. Hypoxia can lead to adaptive responses.For example, angiogenesis will help these cells survive. In this study, we examined the effect of TACE on angiogenesis and expression of vascular endothelial growth factor (VEGF)and basic fibroblast growth factor (b-FGF) and to assess their relevance to Walker-256 transplanted hepatoma.METHODS: Male Wistar rats were inoculated with Walker256 tumor in the left lobe of liver. Angiography and transarterial chemoembolization were performed at d14 after transplantation. Sixty rats bearing walker-256 transplanted hepatoma were randomly divided into control group, arterial infusion group and TACE group. Each group consisted of twenty rats. Normal saline, 5-Fu, 5-Fu and lipiodol were infused through hepatic artery respectively. Two weeks after the infusion, staining of factor Ⅷ, VEGF and b-FGF was performed by immunohistochemistry method in routine paraffin-embeded sections. Microvessel density(MVD) was counted in endothelial cells with positive factor Ⅷ. Their expression levels were analyzed in conjunction with the pathologic features.RESULTS: While a smaller tumor volume was found in TACE group (F=37.818, P<0.001), no statistical differences between MVD and expression of VEGF and b-FGF were found among the 3 groups. MVD of the control group, chemotherapy group and chemoemoblization group was 80.84±24.24,83.05±20.29 and 85.20±23.91 (F=0.193, P=0.873),respectively. The positive expression of VEGF and b-FGF was 75 %, 75 % , 85 % (X2=0.449, P=0.799) and 30 %,25 %, 30 % (X2=0.141, P=0.922), respectively. Statistical analysis revealed a positive correlation between the expression of VEGF and MVD (r=0.552, P<0.001).CONCLUSION: There has been little influence of lipiodol chemoembolization on the formation of tumor angiogenesis,but the development of neovascularization and expressionof VEGF play important roles in establishment of collateralcirculation and reconstruction of blood supply of residualcancer tissue. | Xin Li Gan-Sheng Feng Chuan-Sheng Zheng Chen-Kai Zhuo Xi Liu, Department of Interventional Radiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China | 2003 | World Journal of Gastroenterology2003，9，11： | 20 |
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