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| 1 | DEC1 nuclear expression:A marker of differentiation grade in hepatocellular carcinoma显示文摘AIM: To investigate the expression patterns of human differentiated embryo chondrocyte 1 (DEC1) in hepatocellular carcinoma (HCC) and corresponding adjacent non-tumor and the normal liver tissues, the association between DEC1 expression and histopathological variables and the role of DEC1 in hepatocarcinogenesis. METHODS: The expression of DEC1 was detected immunohistochemically in 176 paraffin-embedded sections from 63 patients with HCC and 50 subjects with normal liver tissues. RESULTS: DEC1 protein was persistently expressed in the cytoplasm of hepatocytes in normal liver and HCC tissues. Compared with adjacent non-tumor liver tissues, HCC tissues showed high nuclear expression of DEC1 protein. However, high DEC1 nuclear expression was more frequently detected in well-differentiated (83.3%) than in moderately (27.3%) and poorly differentiated HCC (16.7%). Low DEC1 expression was associated with poor histological differentiation and malignancy progression. A correlation was found between the nuclear expression of DEC1 protein and histological differentiation (r = 0.376, P = 0.024). CONCLUSION: DEC1 is expressed in the cytoplasm of hepatocytes and because nuclear DEC1 expression is decreased with decreasing differentiation status of HCC, nuclear DEC1 might be a marker of HCC differentiation. | Xiao-Hong Shi Yan Zheng Qing Sun Jing Cui Qing-Hua Liu Fei Qü Yun-Shan Wang | 2011 | World Journal of Gastroenterology2011，17，15： | 15 |
| 2 | Super-resolution dipole orientation mapping via polarization demodulation显示文摘Fluorescence polarization microscopy(FPM)aims to detect the dipole orientation of fluorophores and to resolve structural information for labeled organelles via wide-field or confocal microscopy.Conventional FPM often suffers from the presence of a large number of molecules within the diffraction-limited volume,with averaged fluorescence polarization collected from a group of dipoles with different orientations.Here,we apply sparse deconvolution and least-squares estimation to fluorescence polarization modulation data and demonstrate a super-resolution dipole orientation mapping(SDOM)method that resolves the effective dipole orientation from a much smaller number of fluorescent molecules within a sub-diffraction focal area.We further apply this method to resolve structural details in both fixed and live cells.For the first time,we show that different borders of a dendritic spine neck exhibit a heterogeneous distribution of dipole orientation.Furthermore,we illustrate that the dipole is always perpendicular to the direction of actin filaments in mammalian kidney cells and radially distributed in the hourglass structure of the septin protein under specific labelling.The accuracy of the dipole orientation can be further mapped using the orientation uniform factor,which shows the superiority of SDOM compared with its wide-field counterpart as the number of molecules is decreased within the smaller focal area.Using the inherent feature of the orientation dipole,the SDOM technique,with its fast imaging speed(at sub-second scale),can be applied to a broad range of fluorescently labeled biological systems to simultaneously resolve the valuable dipole orientation information with super-resolution imaging. | Karl Zhanghao Long Chen Xu-San Yang Miao-Yan Wang Zhen-Li Jing Hong-Bin Han Michael Q Zhang Dayong Jin Jun-Tao Gao Peng Xi | 2016 | Light(Science & Applications)2016，5，1： | 11 |
| 3 | Novel functional proteins interact with midkine in hepatic cancer cells显示文摘BACKGROUND: Midkine is a heparin-binding growth factor that promotes the proliferation, survival, migration and differentiation of various target cells. Midkine plays an important role in tumorigenesis and tumor progression, and is overexpressed in many human malignant tumors. Patients with high tumor midkine expression frequently have a worse prognosis than those with low expression. The present study was designed to investigate the interaction network of midkine in hepatic cancer cells, and to elucidate its role in hepatocellular carcinoma. METHODS: DNA encoding full-length midkine was cloned into pDBLeu vector to serve as bait in yeast two-hybrid screening to identify interacting proteins. Candidate proteins were examined on SC-Leu-Trp-His+3-AT (20 mmol/L) plates and assayed for X-gal activity, then sequenced and classified according to the GenBank. Finally, identified proteins were expressed by the in vitro expression system pCMVTnT, and protein interactions were confirmed by co-immunoprecipitation. RESULTS: Using the yeast two-hybrid system, we found 6 proteins that interacted with midkine: NK-kappa-B inhibitor alpha (I-κ-B-α), Dvl-binding protein naked cuticle 2, granulin, latent active TGF-β binding protein 3, latent active TGF-β binding protein 4, and phospholipid scramblase 1. In vitro co-immunoprecipitation demonstrated that all identified proteins directly interacted with midkine.CONCLUSION: The identification of midkine-interacting proteins in hepatic cancer cells indicates that midkine is a multifunctional factor that may participate in cell migration, differentiation, and proliferation, and is also associated with the multicellular response feedback during the development of hepatocellular carcinoma. | Qiang Yan, Hui-Lian Huang, Xing Yao, Jing Li, Li-Qin Li, Jing Zhong, Li-Shan Min, Li-Cheng Dai and Shu-Sen Zheng Department of Hepatobiliary and Pancreatic Surgery, Huzhou Central Hospital (Yan Q and Yao X) Huzhou Key Laboratory of Molecular Medicine, Affiliated Central Hospital of Huzhou Teachers College (Huang HL, Li J, Li LQ, Zhong J, Min LS and Dai LC), Huzhou 313000, China Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Yan Q and Zheng SS) | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，3： | 7 |
| 4 | Expression analysis of a-smooth muscle actin and tenascin-C in the periodontal ligament under orthodontic loading or in vitro culture显示文摘α-smooth muscle actin(α-SMA) and tenascin-C are stress-induced phenotypic features of myofibroblasts. The expression levels of these two proteins closely correlate with the extracellular mechanical microenvironment. We investigated how the expression of α-SMA and tenascin-C was altered in the periodontal ligament(PDL) under orthodontic loading to indirectly reveal the intrinsic mechanical microenvironment in the PDL. In this study, we demonstrated the synergistic effects of transforming growth factor-β1(TGF-β1) and mechanical tensile or compressive stress on myofibroblast differentiation from human periodontal ligament cells(h PDLCs). The h PDLCs under higher tensile or compressive stress significantly increased their levels of α-SMA and tenascin-C compared with those under lower tensile or compressive stress. A similar trend was observed in the tension and compression areas of the PDL under continuous light or heavy orthodontic load in rats. During the time-course analysis of expression, we observed that an increase in α-SMA levels was matched by an increase in tenascin-C levels in the PDL under orthodontic load in vivo. The time-dependent variation of α-SMA and tenascin-C expression in the PDL may indicate the time-dependent variation of intrinsic stress under constant extrinsic loading. | Hui Xu Ding Bai L-Bruno Ruest Jian Q Feng Yong-Wen Guo Ye Tian Yan Jing Yao He Xiang-Long Han | 2015 | International Journal of Oral Science2015，7，4： | 5 |
| 5 | Review of surface photovoltage spectra ofnano sized semiconductor and its applications in heterogeneous photoeatalysis 显示文摘 | Jing L Q Sun X J Shang J | 2003 | Solar Energy Materials And Solar Cells2003，79，2： | 1 |
| 6 | The fates of 1 N fertilizer in relation to root distributions of winter wheat under different N splits 显示文摘 | Shi Z L Jing Q Cai J | 2012 | European Journal of Agronomy2012，40，： | 1 |
| 7 | Adverse reaction and nursing countermeasures in the interferon treatment of chronic hepatitis C显示文摘 | Jing X M Li J R Meng Q | 2001 | J North Sichuan Med CoU2001，16，1： | 1 |
| 8 | TSH/TSHR signaling suppresses fatty acid synthase(FASN) expression in adipocytes显示文摘 | Chen J Ren J Jing Q | 2015 | J Cell Physiol2015，230，9： | 1 |
| 9 | An experiment investigation of heat transfer enhancement by pulsating laminar flow in rectangular grooved channels显示文摘 | LI Y JIN D X JING Y Q | 2013 | Advanced Materials Research2013，732，733： | 1 |
| 10 | The TIL commissioning and performance显示文摘 | X Zhang W Zheng X Wei F Jing Z Sui K Zheng Q Xu X Yuan X Jiang L Yang P Ma M Li J Wang D Hu S He F Li Z Peng B Feng H Zhou L Guo X Li X Zhang J Su Q Zhu H Yu R Zhao C Ma H He D Fan W Zhang | 2008 | Journal of Physics: Conference Series2008，，3： | 1 |
| 11 | Study on high-temperature naphthenic acid corrosion and erosion-corrosion of aluminized carbon steel显示文摘 | Wu X Q Jing H M Zheng Y G | 2004 | Journal of Materials Science2004，39，3： | 1 |
| 12 | Enhanced Activity of Bismuth-Compounded TiO2 Nanoparticles for Photocatalytieally Degrading Rhodamine B Solution显示文摘 | Wang J Jing L Q Xne E P et hi | 2008 | Journal of Hazardous Materials2008，，160： | 1 |
| 13 | The Influence of Fuel Bias in the Primary Air Duct on the Gas/particle Flow Characteristics Near the Swirl Burner Region显示文摘 | Chen Z C Li Z Q Jing J P | 2008 | Fuel Processing Technology2008，89，： | 1 |
| 14 | Anal Chim Acta显示文摘 | Jing T Xia H Guan Q Lu W H Dai Q Niu J W Lim J M Hao Q L Lee Y Zhou Y K Mei S R | 2011 | 692:732011，692，： | 1 |
| 15 | N-glycosylation of acid-sensing ion channel la regulates its trafficking and acidosis-induced spine remodeling显示文摘 | Jing L Chu X P Jiang Y Q | 2012 | J Neurosci2012，32，12： | 1 |
| 16 | Spectrofluorimetric determination of glutathione in human plasma by solid-phase extraction using graphene as adsorbent显示文摘 | Huang K J Jing Q S Wei C Y | 2011 | Spectrochim Acta A2011，79，5： | 1 |
| 17 | Magnetic molecularly imprinted nanoparticles for recognition of lysozyme显示文摘 | Jing T Du H Dai Q | 2010 | Biosensors and Bioelectronics2010，26，2： | 1 |
| 18 | Inflammatory stress increases receptor for lysophosphatidylcholine in human microvascular endothelial cells 显示文摘 | Lum HL Jing Q Robert J | 2003 | Am J Physiol Heart Circ Physiol2003，285，4： | 1 |
| 19 | Resistance of Mo-bearing stainless steels and Mo-bearing stainless-steel coating to naphthenic acid corrosion and erosion-corrosion 显示文摘 | Wu X Q Jing H M Zheng Y G | 2004 | Corrosion Science2004，46，： | 1 |
| 20 | Preparation and characterization of stable biphase TiO2 photocatalyst with high crystallinity, large surface area, and enhanced photoactivity显示文摘 | TIAN G FU H G JING L Q | 2008 | J Phys Chem C2008，112，8： | 1 |
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