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| 1 | 年龄与发育进程问卷上海市儿童常模及心理测量学特性研究显示文摘目的 引进年龄与发育进程问卷(ASQ),研究ASQ中文版(ASQ-C)、ASQ-C上海市3~66个月儿童常模及其心理测量学特性.方法 翻译、回译、改编ASQ成ASQ-C.ASQ-C 19个月龄组、每个月龄组393~517名,共8472名3~66个月常模样本儿童的父母/照看人完成与儿童年龄相应的ASQ-C.其中519名父母/照看人完成ASQ-C后6 d内专业人员再次完成相同ASQ-C,为测试者间信度检验样本;651名父母/照看人间隔10~23 d再次完成相同ASQ-C,为重测信度检验样本;~6、~12、~18、~24、~30个月5个月龄组、每个月龄组42~60名,共255名儿童用贝利婴儿发育量表第2版(BSID Ⅱ)测试作为效度检验样本.采用SPSS 13.0进行统计分析.结果 85.25%儿童的父母/照看人能够独立完成ASQ-C;完成了ASQ-C及其离均差常模;ASQ-C的Cronbach's α系数为0.77;父母/照看人与专业人员分别测试的量表总分间r为0.84(P<0.0001),父母/照看人两次测试的量表总分间r为0.82(P<0.0001);ASQ-C与BSID Ⅱ发育诊断的一致率为84.31%,敏感度为85.00%,特异度为84.26%..结论 上海市儿童的父母/照看人完成ASQ-C可行;ASQ-C具有良好的心理测量学特性,值得进一步研究并引进. | 卞晓燕 姚国英 SQUIRES Jane 魏梅 CHEN Ching-I 方秉华 | 2010 | 中华儿科杂志2010，48，7： | 53 |
| 2 | 他汀类药物在中国冠心病患者中的应用现状调查显示文摘目的:了解国内冠心病患者他汀类药物的使用现状,分析与用药相关的影响因素。方法:借助一项关于调脂治疗的国际多中心大规模临床试验在中国实施之际,于2007-06至2007-12在10座城市的39家三级医院中对符合条件的冠心病患者进行调查,利用电子问卷采集临床资料,对其中的他汀类药物使用情况进行统计描述和分析。结果:符合入选条件的患者共计8161例,其中服用他汀类药物的比例仅为54.1%(4416/8161),服药患者中19.8%(877/4416)持续用药时间明显短于其罹患冠心病的病史时间。患心肌梗死(56.8%对43.1%)、接受冠状动脉旁路移植术(59.3%对53.3%)和经皮冠状动脉腔内成形术(70.5%对38.1%)的患者较之否者服用他汀类药比例高,合并缺血性脑卒中的冠心病患者他汀使用率低于非合并缺血性脑卒中的患者(38.1%对59.2%)。差异均有统计学意义(P<0.001)。各种他汀类药物中,辛伐他汀20~39mg和阿托伐他汀10~19mg使用最为广泛,分别占服用他汀类药物总数的23.9%(1056/4416)和23.3%(1031/4416)。结论:国内冠心病患者目前他汀类药物的使用状况存在明显不足,说明这方面的循证医学证据还亟待转化为科学规范的临床实践。 | 李静 蒋立新 李希 冯芳 CHEN Yi-ping CHEN Zheng-ming LANDRAY Martin ARMITAGE Jane CHEN Fang | 2010 | 中国循环杂志2010，25，5： | 48 |
| 3 | 中国动脉粥样硬化性缺血性脑卒中患者他汀类药物应用现状调查显示文摘目的 了解中国动脉粥样硬化性缺血性脑卒中患者他汀类药物的使用现状,分析影响用药的相关因素.方法 借助一项在中国实施的国际多中心大规模临床试验,从国内10个城市39家三级医院筛查缺血性脑卒中患者5585例,利用电子问卷采集基线资料,对其中他汀类药物使用情况进行统计描述和分析.结果 所有筛查的缺血性脑卒中对象均为他汀类药物适应症者,其中病史在3个月至2年内的患者占60%.研究结果表明,高达80%的调查对象并未服用他汀类药物,即使服药,仍有四分之一的患者用药持续时间明显短于其罹患脑卒中的病史时间.合并冠心病的缺血性脑卒中患者,他汀类药物使用的比例高于单纯脑卒中者3倍.其中以使用辛伐他汀和阿托伐他汀最为广泛.结论 目前国内动脉粥样硬化性缺血性脑卒中患者,他汀类药物的使用严重不足,应予以高度重视. | 蒋立新 | 2010 | 中华流行病学杂志2010，31，8： | 31 |
| 4 | 高危冠心病患者血管紧张素转换酶抑制剂类药物应用现况调查显示文摘目的了解国内部分医院高危冠心病患者血管紧张素转换酶抑NN/血管紧张素Ⅱ受体拮抗剂(ACEI/ARB)类药物的使用现状,分析影响药物使用的相关因素。方法分别于2007年及2009年在14个城市的51家医院进行横断面调查,对符合条件的冠心病患者,利用电子问卷采集基线数据,对ACEI/ARB类冠心病二级预防药物的使用情况进行统计描述和分析。结果在13078例均应使用ACEI/ARB类药物的冠心病患者中,服用ACEI/ARB类药物的比例仅为45.8%,且病史超过1年的患者服药的比例明显下降。即使是合并糖尿病或高血压的患者服药比例也仅分别为46.1%和56.3%。多元logistic回归分析结果显示性别、合并心肌梗死或高血压病史、实施经皮冠状动脉介入治疗及病程长短是影响ACEI/ARB类药物使用的因素。各种ACEI/ARB类药物中,卡托普利使用最为广泛。结论目前国内冠心病患者ACEI/ARB类药物的使用状况存在明显不足,如何将循证医学证据与临床实践有机结合,让尽可能多的患者从中受益,仍是临床工作者及卫生决策部门面临的重大问题。 | 刘佳敏 葛蕾 李静 李希 冯芳 张海波 高岩 乌汉毕力格 庞鑫 CHEN Yi-ping CHEN Zheng-ming LANDRAY Martin ARMITAGE Jane CHEN Fang 蒋立新(代表) HPS2-THRIVE | 2013 | 中华心血管病杂志2013，41，1： | 31 |
| 5 | 抗血小板药物在冠心病患者二级预防中的应用现状调查显示文摘目的了解国内冠心病患者抗血小板药物的使用现状,并分析与用药相关的影响因素。方法借助一项国际多中心大规模临床试验在中国的实施,分别于2007年及2009年在14个城市的51家医院进行调查,对符合条件的冠心病患者,利用电子问卷采集临床资料,对经临床试验证实明确推荐用于二级预防的抗血小板药物使用情况进行统计描述和分析。结果调查的13078例冠心病患者中87.6%服用抗血小板药物,心肌梗死患者服药率为89.2%。既往接受冠状动脉血运重建术患者的抗血小板药物比例较高(94.0%)。各种抗血小板药物中,阿司匹林使用最为广泛,氯吡格雷次之,服药率分别为86.1%和24.6%。冠心病患者行经皮冠状动脉成形术治疗1年内联合使用阿司匹林及氯吡格雷的治疗率为81.0%(2058/2543),其中2009年较2007年有显著增加(86.0%vs.75.9%,P<0.001)。结论本项研究的结果显示国内冠心病患者抗血小板药物的使用与欧美国家使用情况相当,然而在女性及老年人群中抗血小板药物的使用率尚有待改善。 | 乌汉毕力格 李静 李希 冯芳 刘佳敏 高岩 张海波 葛蕾 庞鑫 蒋立新 CHEN Yi-ping CHEN Zheng-ming LANDRAY Martin ARMITAGE Jane | 2012 | 中华临床医师杂志(电子版)2012，6，8： | 22 |
| 6 | CD146 acts as a novel receptor for netrin-1 in promoting angiogenesis and vascular development显示文摘Angiogenesis,最新形成的血容器从先存在发芽的一个过程,为脊椎动物开发和成年动态平衡是重要的。以前的研究证明了 neuronal 指导分子 netrin-1 参予脉管的系统的 angiogenesis 和形态发生。Netrin-1 在 angiogenesis 展出双活动:支持或禁止 angiogenesis。netrin-1 的 anti-angiogenic 活动被 UNC5B 受体调停。然而, netrin-1 怎么支持 angiogenesis,仍然保持不清楚。这里,我们报导那 CD146,免疫球蛋白总科的 endothelial transmembrane 蛋白质,是为 netrin-1 的受体。Netrin-1 与高亲密关系绑在 CD146,导致 endothelial 房间激活并且以一种 CD146 依赖的方式的下游的发信号。在由特定的 anti-CD146 抗体的 netrin-CD146 相互作用的鼠科的内皮细胞层或混乱的 cd146 基因的有条件的大美人堵住或减少 netrin-1-induced angiogenesis。在 zebrafish 胚胎, downregulating 任何一个 netrin-1a 或 CD146 与惹人注目的类似导致脉管的缺点。而且,将宫外的脉管的发芽由 netrin-1 overexpression 导致了的 CD146 块击倒。一起,我们的数据揭开 CD146 在 angiogenesis 为 CD146 作为为 netrin-1 并且也的以前未知的受体揭示功能的 ligand,表明在脊椎动物开发期间在 angiogenesis 发信号的 netrin-CD146 的参与。 | Tao Tu Chunxia Zhang Huiwen Yan Yongting Luo Ruirui Kong Pushuai Wen Zhongde Ye Jianan Chen Jing Feng Feng Liu Jane Y Wu Xiyun Yan | 2015 | Cell Research2015，25，3： | 23 |
| 7 | 中国39家大医院动脉粥样硬化性心脑血管病患者他汀类药物应用现状调查显示文摘目的 了解中国动脉粥样硬化性心脑血管病患者他汀类药物的使用现状,分析影响用药的相关因素.方法 借助一项国际多中心大规模临床试验在中国实施之际,从10个城市的39家三级医院中筛查动脉粥样硬化性心脑血管病调查对象11 783例,利用电子问卷采集基线资料,对其中的他汀类药物使用情况进行统计和分析.结果 接受调查的动脉粥样硬化性心脑血管病患者中,7022例(59.6%)未服用他汀类药物,即使是在4761例服药者中,仍有1120例(23.5%)的调查对象用药持续时间明显短于其罹患动脉粥样硬化性心脑血管病的病史时间.6198例冠心病调查对象(无动脉粥样硬化性缺血性卒中病史)服用他汀的有3668例(59.2%),5585例动脉粥样硬化性缺血性卒中调查对象应用他汀有1093例(19.6%),动脉粥样硬化性缺血性卒中患者的应用比例是冠心病患者的33.1%.结论 目前国内动脉粥样硬化性心脑血管病患者他汀类药物的使用严重不足,尤以动脉粥样硬化性缺血性卒中为甚,应予以高度重视. | 张丹 葛蕾 李静 李希 冯芳 CHEN Yi-ping CHEN Zheng-ming Landray Martin Armitage Jane CHEN Fang 蒋立新 | 2011 | 中华心血管病杂志2011，39，5： | 21 |
| 8 | 中国糖尿病合并动脉粥样硬化性心血管疾病患者他汀类药物的应用现状调查显示文摘目的了解中国糖尿病合并动脉粥样硬化性心血管疾病(CVD)患者他汀类药物的应用现状。方法借助1项国际多中心大规模临床试验在中国实施之际,于2007年6~12月在10个城市的39家三级医院对符合标准的高危动脉粥样硬化性CVD患者进行调查,利用电子问卷采集临床资料,对他汀类药物的使用情况进行统计描述和分析。结果调查4429例糖尿病合并动脉粥样硬化性CVD患者,年龄50~80岁,平均(644-8)岁.66.2%为男性,3345例(75.5%)合并冠心病,2053例(46.4%)合并心肌梗死,1976例(44.6%)合并缺血性脑卒中,3181例(71.8%)合并高血压。仅1952例(44.1%)患者在服用他汀类药物,其中1411例(72.3%)患者未在确诊糖尿病后立即开始服用他汀,而451例(23.1%)患者末在确诊CVD后即开始服用他汀。辛伐他汀(20mg≤且〈40mg)和阿托伐他汀(10mg≤且〈20mg)为最常用剂量,分别占63.5%(489/770)和61.0%(428/702)。结论目前中国糖尿病合并动脉粥样硬化性CVD患者他汀类药物的使用明显不足,应加强对医师的培训和对患者的健康宣教,努力缩小临床章践与循讦医学证据和指南柞荐乡间的荠距. | 蒋立新 李静 冯芳 李希 CHEN Yi-ping CHEN Zheng-ming LANDRAY Martin ARMITAGE Jane CHEN Fang 杨跃进 | 2010 | 中国心血管杂志2010，15，4： | 17 |
| 9 | Currently Clinical Views on Genetics of Wilson's Disease显示文摘 | Chen Chen Bo Shen Jia-Jia Xiao Rong Wu Sarah Jane Duff Canning Xiao-Ping Wang | 2015 | Chinese Medical Journal2015，，13： | 13 |
| 10 | 糖尿病合并动脉粥样硬化性心血管病患者血压控制现状调查显示文摘目的了解中国糖尿病合并动脉粥样硬化性心血管病患者血压控制及降压药物使用情况,分析影响血压控制的因素。方法对参加'第二项心脏保护研究'临床试验筛选门诊的糖尿病合并动脉粥样硬化性心血管病患者进行调查,记录病史和用药情况,测量血压、BMI和腰围。结果 2007年6月至2009年10月期间,在14个城市共调查6522例患者,平均年龄64岁。70%有高血压病史,其中12%未服任何降压药物,仅18%血压达标。缺血性脑卒中病史(OR 0.70,95%CI 0.58~0.84)和向心性肥胖(OR 0.64,95%CI 0.46~0.89)与血压达标呈负相关。无高血压病史的患者中,64%血压高于130/80 mm Hg。结论我国糖尿病合并心血管病患者血压控制情况距指南要求仍存在很大差距,应注意加强对此类患者的血压控制。 | 李静 李希 冯芳 刘佳敏 高岩 张海波 张丹 乌汉毕力格 魏小帅 李丽 葛蕾 庞鑫 CHEN Yi-ping CHEN Zheng-ming LANDRAY Martin ARMITAGE Jane CHEN Fang 蒋立新 | 2011 | 中国糖尿病杂志2011，19，7： | 12 |
| 11 | Use of secondary preventive medications in patients with atherosclerotic disease in urban China: a cross-sectional study of 16 860 patients显示文摘 | LI Jing CHEN Yi-ping LI Xi Jane Armitage FENG Fang LIU Jia-min GAO Yan ZHANG Hai-bo ZHANG Dan HUNDEI Wuhan-bilige CHEN Zheng-ming CHEN Fang Jemma C Hopewell Elsa Valdes-Marquez Martin Landray JIANG Li-xin | 2012 | Chinese Medical Journal2012，，24： | 10 |
| 12 | Underuse of statins in patients with atherosclerotic ischemic stroke in China显示文摘 | LI Xi GAO Yan FENG Fang LIU Jia-min ZHANG Hai-bo ZHANG Dan HUNDEI Wu-han-bi-li-ge CHEN Fang CHEN Yi-ping CHEN Zheng-ming Martin Landray Jane Armitage JIANG Li-xin LI Li-ming | 2012 | Chinese Medical Journal2012，，10： | 9 |
| 13 | Effect of oral epidermal growth factor on mucosal healing in rats with duodenal ulcer显示文摘AIM: To investigate the effect of epidermal growth factor (EGF) on mucosal healing in rats with duodenal ulcer.METHODS: Male Sprague-Dawley rats were randomly divided into sham operation without EGF, sham operation with EGF, duodenal ulcer without EGF, or duodenal ulcer with EGF groups. Additionally, normal rats without operation served as the control group. Duodenal ulcer was induced in rats by 300 mL/L acetic acid. Rats with EGF were orally administered at a dose of 60 μg/kg/day in drinking water on the next day of operation (day 1). Healing of duodenal ulcer was detected by haematoxylin and eosin staining. Cell growth of damaged mucosa was determined by the contents of nucleic acids and proteins. The level of EGF in duodenal mucosa was measured by ELISA.RESULTS: The pathological results showed that duodenal ulcer rats with EGF improved mucosal healing compared with those without EGF after day 5. Duodenal ulcer rats with EGF significantly increased duodenal DNA content compared with those without EGF on day 15 (6.44±0.54mg/g VS 1.45±0.52 mg/g mucosa, P<0.05). Duodenal RNA and protein contents did not differ between duodenal ulcer rats with and without EGF during the experimental period.Sham operation and duodenal ulcer rats with EGF significantly increased duodenal mucosal EGF content compared with those without EGF on day 5 (76.0±13.7 ng/g VS 35.7±12.9ng/g mucosa in sham operation rats, and 68.3±10.9 ng/gVS 28.3±9.2 ng/g mucosa in duodenal ulcer rats, P<0.05).CONCLUSION: Oral EGF can promote mucosal healing of the rats with duodenal ulcer by stimulating mucosal proliferation accompanied by an increase in mucosal EGF content. | Jane CJ Chao Kuo-Yu Liu Sheng-Hsuan Chen Chia-Lang Fang Chih-Wei Tsan | 2003 | World Journal of Gastroenterology2003，9，10： | 9 |
| 14 | Validation of the Physical Activity Questionnaire for Older Children(PAQ-C) among Chinese Children显示文摘Objective This study initially validates the Chinese version of the Physical Activity Questionnaire for Older Children(PAQ-C), which has been identified as a potentially valid instrument to assess moderate-to-vigorous physical activity(MVPA) in children among diverse racial groups. Methods The psychometric properties of the PAQ-C with 742 Hong Kong Chinese children were assessed with the scale's internal consistency, reliability, test-retest reliability, confirmatory factory analysis(CFA) in the overall sample, and multistep invariance tests across gender groups as well as convergent validity with body mass index(BMI), and an accelerometry-based MVPA. Results The Cronbach alpha coefficient(α=0.79), composite reliability value(ρ=0.81), and the intraclass correlation coefficient(α=0.82) indicate the satisfactory reliability of the PAQ-C score. The CFA indicated data fit a single factor model, suggesting that the PAQ-C measures only one construct, on MVPA over the previous 7 days. The multiple-group CFAs suggested that the factor loadings and variances and covariances of the PAQ-C measurement model were invariant across gender groups. The PAQ-C score was related to accelerometry-based MVPA(r=0.33) and inversely related to BMI(r=-0.18). Conclusion This study demonstrates the reliability and validity of the PAQ-C in Chinese children. | WANG Jing Jing BARANOWSKI Tom LAU WC Patrick CHEN Tzu An PITKETHLY Amanda Jane | 2016 | Biomedical and Environmental Sciences2016，29，3： | 7 |
| 15 | 中国冠心病患者血压控制现状调查显示文摘目的了解中国冠心病患者血压控制及降压药物使用情况,分析影响血压控制的因素。方法借助国际多中心大规模临床试验HPS2-THRIVE在中国实施之际,在14座城市的51家医院中对冠心病患者进行调查。利用电子问卷,收集基本情况、病史、吸烟、饮酒和用药资料,测量血压及身高、体质量和腰围,计算达标率。结果 2007年6月至2009年10月期间共调查冠心病患者13 015例,年龄50~80岁,平均(63±8)岁。8033例患者(61.7%)已知明确的高血压病史,其中90.6%服用降压药物,仅30.1%血压控制达标。50~59岁、60~69岁和70~80岁年龄组达标率分别为35.8%、29.3%和25.0%。未服药组、服用1种药物组、服用2种药物组和服用3种及以上组的血压达标率分别为21.1%、26.8%、32.3%和34.9%。大量饮酒者、合并糖尿病或缺血性脑卒中的患者血压达标率(分别为23.8%、19.8%和24.4%)明显低于无此情况者(分别为29.8%、39.5%和32.2%)。相反,接受经皮冠状动脉介入治疗、冠状动脉旁路移植术和服用他汀类药物者血压达标率(分别为35.2%、32.0%和35.2%)均高于无此情况者(分别为25.1%、29.8%和23.2%)。无高血压病史的患者中,仅58.9%血压正常。结论中国冠心病患者血压控制情况距指南要求仍存在很大差距,在大量饮酒以及合并糖尿病或脑卒中的患者中更为突出。 | 李静 李希 冯芳 刘佳敏 高岩 张海波 张丹 乌汉毕力格 魏小帅 李丽 葛蕾 庞鑫 Chen Yi-ping Chen Zheng-ming Landray Martin Armitage Jane Chen Fang 蒋立新 | 2011 | 中国心血管杂志2011，16，3： | 6 |
| 16 | Protective effects of Ginkgo biloba extract on the ethanol-induced gastric ulcer in rats显示文摘AIM: To evaluate the preventive effect of Ginkgo bilobaextract (GbE) on ethanol-induced gastric mucosal injuries in rats.METHODS: Female Wistar albino rats were used for the studies. We randomly divided the rats for each study into five subgroups: normal control, experimental control, and three experimental groups. The gastric ulcers were induced by instilling 1 mL 50% ethanol into the stomach. We gaveGbE 8.75, 17.5, 26.25 mg/kg intravenously to the experimental groups respectively 30 min prior to the ulcerative challenge. We removed the stomachs 45 min later. The gastric ulcers,gastric mucus and the content of non-protein sulfhydryl groups (NP-SH), malondialdehyde (MDA), c-Jun kinase (JNK) activity in gastric mucosa were evaluated. The amount of gastric juice and its acidity were also measured. RESULTS: The findings of our study are as follows: (1)GbE pretreatment was found to provide a dose-dependent protection against the ethanol-induced gastric ulcers in rats; (2) the GbE pretreatment afforded a dose-dependent inhibition of ethanol-induced depletion of stomach wall mucus, NP-SH oontents and increase in the lipid peroxidation (increase MDA) in gastric tissue; (3) gastric ulcer induced by ethanolproduced an increase in JNK activity in gastric mucosawhich also significantly inhibited by pretreatment with GbE;and (4) GbE alone had no inhibitory effect on gastric secretionin pylorus-ligated rats.CONCLUSION: The finding of this study showed that GbE significantly inhibited the ethanol-induced gastric lesions in rats. We suggest that the preventive effect of GbE may be mediated through: (1) inhibition of lipid peroxidation;(2) preservation of gastric mucus and NP-SH; and (3)blockade of cell apoptosis. | Sheng-Hsuan Chen Yu-Chih Liang Jane CJ Chao Li-Hsueh Tsai Chun-Chao Chang Chia-Chi Wang Shiann Pan | 2005 | World Journal of Gastroenterology2005，11，24： | 6 |
| 17 | 年龄与发育进程问卷(中文版)评估上海市3~66月龄儿童神经精神发育春夏秋季的差异显示文摘目的应用'年龄与发育进程问卷:父母完成的儿童发育监测系统'的标准化翻译中文版(ASQ-C),研究上海市3~66月龄儿童在春、夏和秋季神经精神发育的差异。方法于春、夏和秋季,在上海市3~66月龄儿童中分层随机整群抽样。共有8472名儿童的父母和(或)养育人完成了儿童月龄相应的ASQ-C测试。儿童ASQ-C的量表总分及沟通(CM)、大运动(GM)、精细运动(FM)、解决问题(CG)和个人-社会(PS)能区得分以x珋±s表示;多组样本均数的比较采用完全随机设计的单因素方差分析;两两比较采用SNK法。采用多元线性回归分析以排除混杂因素。结果①不同季节测试儿童的ASQ-C量表总分间差异有统计学意义(P<0.01),从高至低的排序是秋、夏和春季,秋季显著高于夏和春季(P<0.05),夏季与春季间差异无统计学意义;②不同季节测试儿童的ASQ-C5个能区得分间差异均有统计学意义(P<0.01或<0.05),均以秋季最高;其中CM能区得分从高至低的排序为秋、春和夏季,其他4个能区得分从高至低的排序均为秋、夏和春季。两两比较结果显示,CM能区得分秋季显著高于夏季(P<0.05),秋季与春季、春季与夏季间差异无统计学意义;其他4个能区得分均表现为秋季显著高于夏和春季(P<0.05)。结论季节对3~66月龄儿童的神经精神发育有影响;进一步研究季节或气候与儿童发育的关系很有必要;在儿童发育评估量表的研究中,建议考虑季节因素的影响。 | 魏梅 SQUIRES Jane 宋魏 喻茜 姚国英 卞晓燕 张莉 郑小斐 CHEN Ching-I 徐素香 | 2011 | 中国循证儿科杂志2011，6，6： | 5 |
| 18 | 恶性肿瘤患者特质心理韧性量表的科学性考评显示文摘目的:对前期开发的恶性肿瘤患者特质心理韧性量表(RS-SC)进行临床检验。方法:对250例来自广州中医药大学第一附属医院、广东省中医院的恶性肿瘤患者进行问卷调查,采用双参数项目反应理论(IRT)模型对RS-SC进行分离指数、阈值、区分度、残差拟合度等参数进行检验。结果:RS-SC仍保留25条目的三维度结构,各维度的Cronbach’sα为0.814-0.851,条目分离指数为0.891-0.945,累积方差解释量为67.38%;25个条目难度分布均匀,区分度在0.34-0.99之间,残差拟合度都在-2.5~2.5之间,阈值间距在0.06-6.95 logits之间。结论:RS-SC具有良好的三维度结构,各条目的信息拟合度较高(部分条目需要进行后期调整),适合在中国恶性肿瘤患者中使用。 | 叶增杰 梁木子 余远亮 Knobf Tish Dixon Jane Chen Wei-Ti 邱鸿钟 | 2018 | 中华中医药杂志2018，33，5： | 5 |
| 19 | MicroRNAs as therapeutic strategy for hepatitis B virusassociated hepatocellular carcinoma: Current status and future prospects显示文摘Hepatocellular carcinoma(HCC)remains to be one of the top causing cancer-related deaths today.The majority of HCC cases are reported to be the result of chronic hepatitis B virus(HBV)infection.Current treatments for HBV-related HCC revolve around the use of drugs to inhibit viral replication,as a high level of viral load and antigen in circulation often presents a poor patient prognosis.However,existing therapies are inefficient in the complete eradication of HBV,often resulting in tumour recurrence.The involvement of microRNAs(miRNAs)in important processes in HBV-related HCC makes it an important player in the progression of HCC in chronic hepatitis B infected patients.In this review,we discuss the key aspects of HBV infection and the important viral products that may regulate cancerrelated processes via their interaction with miRNAs or their closely related protein machinery.Conversely,we also look at how miRNAs may go about regulating the virus,especially in vital processes like viral replication.Apart from miRNAs acting as either oncogenes or tumour-suppressors,we also look at how miRNAs may function as biomarkers that may possibly serve as better candidates than those currently employed in the diagnosis of HBV infection or HBV-related HCC.A summary of the roles of miRNAs in HBV-related HCC will hopefully lead to a gain in understanding of the pathogenesis process and pave the way for new insights in medical therapy. | Yi Lin Jane Tan Wei Ning Chen | 2014 | World Journal of Gastroenterology2014，20，20： | 4 |
| 20 | Expression of human FUS/TLS in yeast leads to protein aggregation and cytotoxicity,recapitulating key features of FUS proteinopathy显示文摘Mutations in the fused in sarcoma/translocated in liposarcoma(FUS/TLS)gene have been associated with amyotrophic lateral sclerosis(ALS).FUS-positive neuropathology is reported in a range of neurodegenerative diseases,including ALS and fronto-temporal lobar degeneration with ubiquitin-positive pathology(FTLDU).To examine protein aggregation and cytotoxicity,we expressed human FUS protein in yeast.Expression of either wild type or ALS-associated R524S or P525L mutant FUS in yeast cells led to formation of aggregates and cytotoxicity,with the two ALS mutants showing increased cytotoxicity.Therefore,yeast cells expressing human FUS protein recapitulate key features of FUSpositive neurodegenerative diseases.Interestingly,a significant fraction of FUS expressing yeast cells stained by propidium iodide were without detectable protein aggregates,suggesting that membrane impairment and cellular damage caused by FUS expression may occur before protein aggregates become microscopically detectable and that aggregate formation might protect cells from FUS-mediated cytotoxicity.The N-terminus of FUS,containing the QGSY and G rich regions,is sufficient for the formation of aggregates but not cytotoxicity.The C-terminal domain,which contains a cluster of mutations,did not show aggregation or cytotoxicity.Similar to TDP-43 when expressed in yeast,FUS protein has the intrinsic property of forming aggregates in the absence of other human proteins.On the other hand,the aggregates formed by FUS are thioflavin T-positive and resistant to 0.5%sarkosyl,unlike TDP-43 when expressed in yeast cells.Furthermore,TDP-43 and FUS display distinct domain requirements in aggregate formation and cytotoxicity. | Kazuo Fushimi Charles Long Neha Jayaram Xiaoping Chen Liming Li Jane Y.Wu | 2011 | Protein & Cell2011，2，2： | 4 |
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