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| 1 | Metal alginates for polyphenol delivery systems: Studies on crosslinking ions and easy-to-use patches for release of protective flavonoids in skin显示文摘Incorporation of bioactive natural compounds like polyphenols is an attractive approach for enhanced functionalities of biomaterials.In particular flavonoids have important pharmacological activities,and controlled release systems may be instrumental to realize the full potential of these phytochemicals.Alginate presents interesting attributes for dermal and other biomaterial applications,and studies were carried here to support the development of polyphenol-loaded alginate systems.Studies of capillary viscosity indicated that ionic medium is an effective strategy to modulate the polyelectrolyte effect and viscosity properties of alginates.On gelation,considerable differences were observed between alginate gels produced with Ca^2+,Ba^2+,Cu^2+,Fe^2+,Fe^3+and Zn^2+as crosslinkers,especially concerning shrinkage and morphological regularity.Stability assays with different polyphenols in the presence of alginate-gelling cations pointed to the choice of calcium,barium and zinc as safer crosslinkers.Alginate-based films loaded with epicatechin were prepared and the kinetics of release of the flavonoid investigated.The results with calcium,barium and zinc alginate matrices indicated that the release dynamics is dependent on film thicknesses,but also on the crosslinking metal used.On these grounds,an alginate-based system of convenient use was devised,so that flavonoids can be easily loaded at simple point-of-care conditions before dermal application.This epicatechin-loaded patch was tested on an ex-vivo skin model and demonstrated capacity to deliver therapeutically relevant concentrations on skin surface.Moreover,the flavonoid released was not modified and retained full antioxidant bioactivity.The alginate-based system proposed offers a multifunctional approach for flavonoid controllable delivery and protection of skin injured or under risk. | João Silva Pavlo Vanat Dorinda Marques-da-Silva Joaquim Rui Rodrigues Ricardo Lagoa | 2020 | Bioactive Materials2020，5，3： | 2 |
| 2 | Impaired T-cell differentiation in diabetic foot ulceration显示文摘Foot ulceration is one of the most debilitating complications associated with diabetes,but its cause remains poorly understood.Several studies have been undertaken to understand healing kinetics or find possible therapies to enhance healing.However,few studies have been directed at understanding the immunological alterations that could influence wound healing in diabetes.In this study,we analysed the T-cell receptor(TCR)repertoire diversity in TCR-αβ^(+)T cells.We also analysed the distribution and phenotype of T cells obtained from the peripheral blood of healthy controls and diabetic individuals with or without foot ulcers.Our results showed that diabetic individuals,especially those with foot ulcers,have a significantly lower naive T-cell number and a poorer TCR-Vβrepertoire diversity.We also showed that the reduced TCR-Vβrepertoire diversity in diabetic individuals was mainly owing to the accumulation of effector T cells,the major source of tumour necrosis factor-αproduction,which was even more pronounced in patients with acute foot ulceration.Moreover,the expression of several inflammatory chemokine receptors was significantly reduced in diabetic patients.In conclusion,effector T-cell accumulation and TCR repertoire diversity reduction appear to precede the development of foot ulcers.This finding may open new immunological therapeutic possibilities and provide a new prognostic tool in diabetic wound care. | João Moura João Rodrigues Marta Gonçalves Cláudia Amaral Margarida Lima Eugénia Carvalho | 2017 | Cellular & Molecular Immunology2017，14，9： | 6 |
| 3 | Dexamethasone intravitreal implant(Ozurdex) in diabetic macular edema: real-world data versus clinical trials outcomes显示文摘AIM: To investigate the safety and efficacy of intravitreal dexamethasone implants(Ozurdex?/DEX) in patients with diabetic macular edema(DME) either na?ve or nonna?ve to anti-VEGF therapies who switched to DEX implant independent of response to anti-vascular endothelial growth factors(anti-VEGFs).METHODS: This was an audit retrospective review of medical records of patients with DME who switched to the DEX intravitreal implant. Patients were divided into 2 groups: patients na?ve to antiangiogenic therapy and patients who were previously treated with anti-VEGFs. Data regarding demographics, changes in mean best-corrected visual acuity(BCVA), central macular thickness(CMT), and intraocular pressure(IOP) was collected over 6 mo. The demographic data mean changes in BCVA, CMT, and IOP were compared. Six-month follow-up data of 47 patients(57 eyes), who either switched to DEX implant irrespective of response to previous treatments or were treatment na?ve before receiving DEX implant, was collected.RESULTS: Improvement in mean BCVA was observed from 1-4 mo after injection with a decreased effect at month 6 as expected, with better outcomes in na?ve compared to non-na?ve patients. A statistically relevant decrease in mean CMT was observed during the follow-up period. An increase in mean IOP was observed in the first 2 mo after DEX therapy. The mean number of injections of the overall population during the 6 mo was 1.3. A subgroup analysis showed no relevant difference between phakic versus pseudophakic patients relative to measured outcomes. There was no cataract progression during the follow-up period and no adverse events reported.CONCLUSION: This real-life setting study shows that intravitreal DEX implant is effective and safe. The timings of greater therapeutic impact are concordant with previous studies and suggest that earlier treatment with corticosteroids may have an additional benefit in na?ve patients. | Pedro Neves Mário Ornelas Inês Matias João Rodrigues Margarida Santos Marco DutraMedeiros David Martins | 2021 | International Journal of Ophthalmology(English edition)2021，14，10： | 1 |
| 4 | Comparing the Behavior of Reinforced Concrete Columns Embedded in Walls and Subjected to Fire显示文摘Fire is an exceptional action that may occur during the life of a building.So,it must be considered when designing a building structure.The standards provide several types of design methods for that propose,used for single elements,parts of structure or the structure as a whole.The fire design of columns is important both for new project as for remodel buildings and also for verification of the residual resistance of columns that have suffered a fire accident.In this way,the aim of this work is to analyze numerically different ways of fire exposure to check the compressive strength of the columns when subjected to fire and the influence of the adjacent walls to a column in case of fire.The thermal advanced analysis of the sections columns was performed using the finite element software,Abaqus CAE,where the standard fire curve,ISO 834(International Organization for Standardization 834),was used,with 4 h of fire duration.It was possible,with the two methods used in this work,to compare them to verify which model is more conservative and which is closer to the advanced numerical model,for calculating temperatures in the column section.It was checked that the walls act as thermal insulators,protecting part of the columns from the convection and radiation of the fire.Consequently,the effects of raising the temperature over the compressive resistance of the reinforced concrete column,were reduced. | Bruno Carvalho Lima de Alencar Matos Fernando Luis da Silva Ferreira João Paulo Correia Rodrigues Rodrigo Barreto Caldas | 2022 | Journal of Civil Engineering and Architecture2022，16，7： | 0 |
| 5 | Polysaccharide-based nanomedicines for cancer immunotherapy:A review显示文摘Cancer immunotherapy is an effective antitumor approach through activating immune systems to eradicate tumors by immunotherapeutics.However,direct administration of“naked”immunotherapeutic agents(such as nucleic acids,cytokines,adjuvants or antigens without delivery vehicles)often results in:(1)an unsatisfactory efficacy due to suboptimal pharmacokinetics;(2)strong toxic and side effects due to low targeting(or off-target)efficiency.To overcome these shortcomings,a series of polysaccharide-based nanoparticles have been developed to carry immunotherapeutics to enhance antitumor immune responses with reduced toxicity and side effects.Polysaccharides are a family of natural polymers that hold unique physicochemical and biological properties,as they could interact with immune system to stimulate an enhanced immune response.Their structures offer versatility in synthesizing multifunctional nanocomposites,which could be chemically modified to achieve high stability and bioavailability for delivering therapeutics into tumor tissues.This review aims to highlight recent advances in polysaccharide-based nanomedicines for cancer immunotherapy and propose new perspectives on the use of polysaccharide-based immunotherapeutics. | Yujun Zeng Yufan Xiang Ruilong Sheng Helena Tomás João Rodrigues Zhongwei Gu Hu Zhang Qiyong Gong Kui Luo | 2021 | Bioactive Materials2021，6，10： | 3 |
| 6 | Understanding how environmental heterogeneity and elevation drives the distribution of woody communities across vegetation types within the campo rupestre in South America显示文摘The campo rupestre sensu lato is a vegetation type that occurs in South American mountains,supports a distinctive flora characterized by high rates of endemism,high herbaceous species richness and often-neglected but also species-rich of the arboreal stratum.We aimed to investigate how environmental factors and elevation are associated with the distribution and diversity of woody species in different rupestrian vegetation types across South America.Using a database of 2,049 woody species from 185 sites across four vegetation types within the campo rupestre,we assessed how the vegetation types were grouped according to their floristic composition and number of shared indicator species,as well as by using different beta diversity indices.The most important variables from a set of 27 variables(e.g.altitude,geo-edaphic and climatic)explaining species distribution were identified using redundancy analysis(RDA)and variation partitioning methods.The distribution of vegetation types was related to both environmental and spatial fractions,with a set of 17 variables retained(e.g.rockiness,grass cover and temperature seasonality as the most important variables).There was an association between the floristic composition of each vegetation type and the elevation range.Although the identified vegetation types are floristically related,they are distinguished by exclusive and habitat-specialist woody species.This uniqueness of vegetation types should be considered in terms of complementarity for the conservation of campos rupestres. | Marcelo Leandro BUENO Vanessa Leite REZENDE Luiza Fonseca A.DE PAULA João Augusto Alves MEIRA-NETO JoséRoberto Rodrigues PINTO Andreza Viana NERI Vanessa PONTARA | 2021 | Journal of Mountain Science2021，18，5： | 0 |
| 7 | Prevalence of Kudoa in Fish Fillets Caught in Para State显示文摘Kudoa is a myxozoan that causes myoliquefaction in marine fishes.Most of species only affect fish,but a K.septempunctata outbreak was reported in 358 people.Although many species of Kudoa are known,none was described in Brachyplatystoma filamentosum,Brachyplatystoma rousseauxii,Mugil curema,Plagioscion squamosissimus or Oxydoras niger until now.Due to the economic cost of eliminating seafood presenting myxozoan lesions,this study aimed to describe lesions found at necropsy and histopathology,as well as to detect this myxozoan by molecular techniques.For this purpose,were sampled 85 fish of the following species:Brachyplatystoma filamentosum,Brachyplatystoma rousseauxii,Mugil curema,Plagioscion squamosissimus,and Oxydoras niger from Colares and Vigia,Pará,Brazil.Necropsies were carried out to describe lesions and molecular techniques(PCR and sequencing)were applied for identification.Although muscle lesions were not observed at necropsy,histopathology revealed bacterial colonies,coagulative necrosis,dystrophic calcification,eosinophils,hemorrhage,parasitic pseudocysts,protozoan,and vacuolization.After sequencing,K.shiomitsui(GENBANK:LC128646)was identified as the most similar causative agent of fishes infection,but due to phylogenetic results and identity we suggest that the myxozoan found could be a new specie.Also,high parasitism of this myxozoan was observed in fishes sampled,i.e.,90%in Colares and 100%in Vigia. | Marianna Vaz RODRIGUES Patrícia Tidori MIURA Jéssica Fernandes de OLIVEIRA Maria das Dores Correia Palha João Pessoa Araújo JÚNIOR | 2020 | Journal of Zoological Research2020，2，3： | 0 |
| 8 | Development and challenges of cells-and materials-based tooth regeneration显示文摘Tooth defect and loss are common clinical diseases in stomatology.With the extension of life expectancy,there is an increasing demand for tooth tissue and whole tooth regeneration.Compared with traditional oral prosthetic treatment,tooth regeneration has unique advantages and has become one of the hotspots towards oral biomedical treatment.In this review,we discussed the development and challenges of tooth regeneration based on cells and materials,including tooth enamel,dentin,dental pulp,cementum,dentin-pulp complex,and the whole tooth regeneration,in order to provide a comprehensive,up-to-date,illustrative overview of tooth regeneration issues.The mechanisms of the regeneration were also summarized and discussed.Moreover,this review hints the future perspective and research direction of tooth regeneration in the challenging field of regenerative dentistry. | Zeyu Fu Yu Zhuang Jinjie Cui Ruilong Sheng Helena Tomás João Rodrigues Bin Zhao Xudong Wang Kaili Lin | 2022 | Engineered Regeneration2022，3，2： | 0 |
| 9 | NT157 has antineoplastic effects and inhibits IRS1/2 and STAT3/5 in JAK2^(V617F)-positive myeloproliferative neoplasm cells显示文摘Recent data indicate that IGF1R/IRS signaling is a potential therapeutic target in BCR-ABL1-negative myeloproliferative neoplasms(MPN);in this pathway,IRS2 is involved in the malignant transformation induced by JAK2^(V617F),and upregulation of IGF1R signaling induces the MPN phenotype.NT157,a synthetic compound designed as an IGF1R-IRS1/2 inhibitor,has been shown to induce antineoplastic effects in solid tumors.Herein,we aimed to characterize the molecular and cellular effects of NT157 in JAK2^(V617F)positive MPN cell lines(HEL and SET2)and primary patient hematopoietic cells.In JAK2^(V617F)cell lines,NT157 decreased cell viability,clonogenicity,and cell proliferation,resulting in increases in apoptosis and cell cycle arrest in the G2/M phase(p<0.05).NT157 treatment inhibited IRS1/2,JAK2/STAT,and NFκB signaling,and it activated the AP-1 complex,downregulated four oncogenes(CCND1,MYB,WT1,and NFKB1),and upregulated three apoptotic-related genes(CDKN1A,FOS,and JUN)(p<0.05).NT157 induced genotoxic stress in a JAK2/STAT-independent manner.NT157 inhibited erythropoietin-independent colony formation in cells from polycythemia vera patients(p<0.05).These findings further elucidate the mechanism of NT157 action in a MPN context and suggest that targeting IRS1/2 proteins may represent a promising therapeutic strategy for MPN. | Bruna Alves Fenerich Jaqueline Cristina Fernandes Ana Paula Nunes Rodrigues Alves Juan Luiz Coelho-Silva Renata Scopim-Ribeiro Priscila Santos Scheucher Christopher A.Eide Cristina E.Tognon Brian J.Druker Eduardo Magalhães Rego João Agostinho Machado-Neto Fabiola Traina | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 2 |
| 10 | Is magnesium deficiency the major cause of needle chlorosis of Pinus taeda in Brazil?显示文摘Needle chlorosis(NC)in Pinus taeda L.systems in Brazil becomes more frequent after second and third harvest rotation cycles.In a study to identify factors contributing to yellowing needle chorosis(YNC),trees were grown in soils originating from contrasting parent materials,and soils and needles(whole,green and chlorotic portions)from 1-and 2-year-old branches and the first and second needle flush release at four sites with YNC on P.taeda were analyzed for various elements and properties.All soils had very low base levels(Ca^(2+),Mg^(2+)and K^(+))and P,suggesting a possible lack of multiple elements.YNC symptoms started at needle tips,then extended toward the needle base with time.First flush needles had longer portions with YNC than second flush needles did.Needles from the lower crown also had more symptoms along their length than those higher in the canopy.Symptoms were similar to those reported for Mg.In chlorotic portions,Mg and Ca concentrations were well below critical values;in particular,Mg levels were only one third of the critical value of 0.3 g kg^(-1).Collectively,results suggest that Mg deficiency is the primary reason for NC of P.taeda in various parent soils in Brazil. | Antônio Carlos Vargas Motta Shizuo Maeda Valdécio dos Santos dos Santos Rodrigues Tamires Maiara Ercole Stephen Arthur Prior Ana Elisa Lyra Brumat Amanda Pacheco Cardoso Moura Julierme Zimmer Barbosa João Bosco Vasconcellos Gomes | 2024 | Journal of Forestry Research2024，35，2： | 0 |
| 11 | Remodeling of the cell wall as a drought-tolerance mechanism of a soybean genotype revealed by global gene expression analysis显示文摘Drought stress is major abiotic stress that affects soybean production.Therefore,it is widely desirable that soybean becomes more tolerant to stress.To provide insights into regulatory mechanisms of the stress response,we compared the global gene expression profiles from leaves of two soybean genotypes that display different responses to water-deficit(BR 16 and Embrapa 48,drought-sensitive and droughttolerant,respectively).After the RNA-seq analysis,a total of 5335 down-regulated and 3170 up-regulated genes were identified in the BR16.On the other hand,the number of genes differentially expressed was markedly lower in the Embrapa 48,355 up-regulated and 471 down-regulated genes.However,induction and expression of protein kinases and transcription factors indicated signaling cascades involved in the drought tolerance.Overall,the results suggest that the metabolism of pectin is differently modulated in response to drought stress and may play a role in the soybean defense mechanism against drought.This occurs via an increase of the cell wall plasticity and crosslink,which contributed to a higher hydraulic conductance(Kf)and relative water content(RWC%).The drought-tolerance mechanism of the Embrapa 48 genotype involves remodeling of the cell wall and increase of the hydraulic conductance to the maintenance of cell turgor and metabolic processes,resulting in the highest leaf RWC,photosynthetic rate(A),transpiration(E)and carboxylation(A/Ci).Thus,we concluded that the cell wall adjustment under drought is important for a more efficient water use which promoted a more active photosynthetic metabolism,maintaining higher plant growth under drought stress. | Flaviane Silva Coutinho Juliano Mendonça Rodrigues Lucas Leal Lima Rosilene Oliveira Mesquita Paola Avelar Carpinetti João Paulo Batista Machado Camilo Elber Vital Pedro Marcus Vidigal Maria Eduarda Soares Ramos Mariana Rocha Maximiano Angela Mehta Maria Goreti Almeida Oliveira Elizabeth Pacheco Batista Fontes Humberto Josuéde Oliveira Ramos | 2021 | aBIOTECH2021，2，1： | 0 |
| 12 | Can gadoxetic acid–enhanced magnetic resonance imaging be used to avoid liver biopsy in patients with nonalcoholic fatty liver disease?显示文摘BACKGROUND Nonalcoholic fatty liver disease(NAFLD)is a major cause of liver disease worldwide.The diagnosis of nonalcoholic steatohepatitis(NASH),the most severe form of NAFLD,is crucial and has prognostic and therapeutic implications.However,currently this diagnosis is based on liver biopsy and has several limitations.AIM To evaluate the performance of gadoxetic acid–enhanced magnetic resonance imaging(GA-MRI)in differentiating isolated steatosis from NASH in patients with NAFLD.METHODS In this prospective study,56 patients with NAFLD(18 with isolated steatosis and 38 with NASH)underwent GA-MRI.The contrast enhancement index(CEI)was calculated as the rate of increase of the liver-to-muscle signal intensity ratio from before and 20 min after intravenous GA administration.Between-group differences in mean CEI were examined using Student's t test.The area under the receiver operator characteristic curve and the diagnostic performance of gadoxetic acid–enhanced magnetic resonance imaging were evaluated.RESULTS The mean CEI for all subjects was 1.82±0.19.The mean CEI was significantly lower in patients with NASH than in those with isolated steatosis(P=0.008).Two CEI cut-off points were used:<1.66(94%specificity)to characterize NASH and>2.00(89%sensitivity)to characterize isolated steatosis.CEI values between 1.66 and 2.00 indicated liver biopsy,and the procedure could be avoided in 40%of patients with NAFLD.CONCLUSION GA-MRI is an effective noninvasive method that may be useful for the differentiation of NASH from isolated steatosis,and could help to avoid liver biopsy in patients with NAFLD. | Viviane Brandão Amorim Daniella Braz Parente Fernando Fernandes Paiva Jaime Araújo Oliveira Neto Amanda Almeida Miranda Cláudia Cravo Moreira Flávia Ferreira Fernandes Carlos Frederico FerreiraCampos Nathalie Carvalho Leite Renata de Mello Perez Rosana Souza Rodrigues | 2020 | World Journal of Hepatology2020，12，9： | 0 |
| 13 | 基于溶液化学策略构建背接触FTO/赤铁矿光阳极界面工程的高效光催化水氧化显示文摘本文采用一种简单、有效的规模化溶液化学策略,在基底(如商用氟掺杂氧化锡透明导电涂层玻璃(FTO))和光活性薄膜(如赤铁矿)之间形成丰富的背接触界面,并用于低成本水氧化反应.高分辨率电子显微镜(扫描电镜、透射电镜、扫描透射电镜)、原子力显微镜、元素成像(电子能量损失谱和能量色散谱)和光电化学研究表明,可通过前驱体溶液的化学成分工程来有效降低机械应力、晶格失配、电子势垒和FTO与赤铁矿在背面接触界面之间的空隙以及FTO与电解液之间的短路和有害反应,进而提升这些低成本光阳极对水氧化反应以及PEC水分解清洁、可持续地生产氢气的整体效率.本研究对通过最小化在介孔电极的背接触界面和晶粒边界上的电子-空穴复合,进而提高电荷收集效率具有重要意义,可提高低成本PEC水裂解装置的整体效率和规模化的能力. | Karen Cristina Bedin Beatriz Mouriño Ingrid Rodríguez-Gutiérrez João Batista Souza Junior Gabriel Trindade dos Santos Jefferson Bettini Carlos Alberto Rodrigues Costa Lionel Vayssieres Flavio Leandro Souza | 2022 | Chinese Journal of Catalysis2022，43，5： | 0 |
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