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| 1 | Green fluorescent protein (GFP) transgenic pig produced by somatic cell nuclear transfer显示文摘Transgenic somatic cell nuclear transfer is a very promising route for producing transgenic farm ani-mals. Research on GFP transgenic pigs can provide useful information for breeding transgenic pigs, human disease models and human organ xenotransplantation. In this study, a liposomal transfection system was screened and transgenic embryos were reconstructed by nuclear transfer of GFP positive cells into enucleated in vitro matured oocytes. The development of reconstructed embryos both in vitro and in vivo was observed, and GFP expression was determined. The results showed that porcine fe-tal-derived fibroblast cells cultured with 4.0 μL/mL liposome and 1.6 μg/mL plasmid DNA for 6 h re-sulted in the highest transfection rate (3.6%). The percentage of GFP reconstructed embryos that de-veloped in vitro to the blastocyst stage was 10%. Of those the GFP positive percentage was 48%. Re-constructed transgenic embryos were transferred to 10 recipients. 5 of them were pregnant, and 3 de-livered 6 cloned piglets in which 4 piglets were transgenic for the GFP as verified by both GFP protein expression and GFP DNA sequence analysis. The percentage of reconstructed embryos that resulted in cloned piglets was 1.0%; while the percentage of piglets that were transgenic was 0.7%. This is the first group of transgenic cloned pigs born in China, marking a great progress in Chinese transgenic cloned pig research. | LIU ZhongHua SONG Jun WANG ZhenKun TIAN JiangTian KONG QingRan ZHENG Zhong YIN Zhi GAO Li MA HaiKun SUN Shuang LI YuTian WANG HongBin R S PRATHER | 2008 | Chinese Science Bulletin2008，53，7： | 8 |
| 2 | High-resolution microendoscopy for esophageal cancer screening in China: A cost-effectiveness analysis显示文摘AIM:To study the cost-effectiveness of high-resolution microendoscopy(HRME)in an esophageal squamous cell carcinoma(ESCC)screening program in China.METHODS:A decision analytic Markov model of ESCC was developed.Separate model analyses were conducted for cohorts consisting of an averagerisk population or a high-risk population in China.Hypothetical 50-year-old individuals were followed until age 80 or death.We compared three different strategies for both cohorts:(1)no screening;(2)standard endoscopic screening with Lugol’s iodine staining;and(3)endoscopic screening with Lugol’s iodine staining and an HRME.Model parameters were estimated from the literature as well as from GLOBOCAN,the Cancer Incidence and Mortality Worldwide cancer database.Health states in the model included non-neoplasia,mild dysplasia,moderate dysplasia,high-grade dysplasia,intramucosal carcinoma,operable cancer,inoperable cancer,and death.Separate ESCC incidence transition rates were generated for the average-risk and high-risk populations.Costs in Chinese currency were converted to international dollars(I$)and were adjusted to 2012dollars using the Consumer Price Index.RESULTS:The main outcome measurements for this study were quality-adjusted life years(QALYs)and incremental cost-effectiveness ratio(ICER).For the average-risk population,the HRME screening strategy produced 0.043 more QALYs than the no screening strategy at an additional cost of I$646,resulting in an ICER of I$11808 per QALY gained.Standard endoscopic screening was weakly dominated.Among the high-risk population,when the HRME screening strategy was compared with the standard screening strategy,the ICER was I$8173 per QALY.For both the high-risk and average-risk screening populations,the HRME screening strategy appeared to be the most cost-effective strategy,producing ICERs below the willingness-topay threshold,I$23500 per QALY.One-way sensitivity analysis showed that,for the average-risk population,higher specificity of Lugol’s iodine(>40%)and lower specificity of HRME(<70%)could make Lugol’s iodine screening cost-effective.For the high-risk population,the results of the model were not substantially affected by varying the follow-up rate after Lugol’s iodine screening,Lugol’s iodine test characteristics(sensitivity and specificity),or HRME specificity.CONCLUSION:The incorporation of HRME into an ESCC screening program could be cost-effective in China.Larger studies of HRME performance are needed to confirm these findings. | Chin Hur Sung Eun Choi Chung Yin Kong Gui-Qi Wang Hong Xu Alexandros D Polydorides Li-Yan Xue Katherine E Perzan Angela C Tramontano Rebecca R Richards-Kortum Sharmila Anandasabapathy | 2015 | World Journal of Gastroenterology2015，21，18： | 8 |
| 3 | Primary squamous cell carcinoma of the rectum: An update and implications for treatment显示文摘AIM: To provide an update on the aetiology, pathogenesis, diagnosis, staging and management of rectal squamous cell carcinoma(SCC).METHODS: A systematic review was conducted according to the preferred reporting items for Systematic Reviews and Meta-Analyses guidelines. A comprehensive search of Ovid MEDLINE was performed with the reference list of selected articles reviewed to ensure all relevant publications were captured. The search strategy was limited to the English language, spanning from 1946 to 2015. A qualitative analysis was undertaken examining patient demographics, clinical presentation, diagnosis, staging, treatment and outcome. The quantitaive analysis was limited to data extracted on treatment and outcomes including radiological, clinical and pathological complete response where available. The narrative and quantitative review were synthesised in concert.RESULTS: The search identified 487 articles in total with 79 included in the qualitative review. The quantitative analysis involved 63 articles, consisting of 43 case reports and 20 case series with a total of 142 individual cases. The underlying pathogenesis of rectal SCC while unclear, continues to be defined, with increasing evidence of a metaplasia-dysplasia-carcinoma sequence and a possible role for human papilloma virus in this progression. The presentation is similar to rectal adenocarcinoma, with a diagnosis confirmed by endoscopic biopsy. Many presumed rectal SCC's are in fact an extension of an anal SCC, and cytokeratin markers are a useful adjunct in this distinction. Staging is most accurately reflected by the tumour-nodemetastasis classification for rectal adenocarcinoma. It involves examining locoregional disease by way of magnetic resonance imaging and/or endorectal ultrasound, with systemic spread excluded by way of computed tomography. Positron emission tomography is integral in the workup to exclude an external siteof primary SCC with metastasis to the rectum. While the optimal treatment remains as yet undefined, recent studies have demonstrated a global shift away from surgery towards definitive chemoradiotherapy as primary treatment. Pooled overall survival was calculated to be 86% in patients managed with chemoradiation compared with 48% for those treated traditionally with surgery. Furthermore, local recurrence and metastatic rates were 25% vs 10% and 30% vs 13% for the chemoradiation vs conventional treatment cohorts.CONCLUSION: The changing paradigm in the treatment of rectal SCC holds great promise for improved outcomes in this rare disease. | Glen R Guerra Cherng H Kong Satish K Warrier Andrew C Lynch Alexander G Heriot Samuel Y Ngan | 2016 | World Journal of Gastrointestinal Surgery2016，8，3： | 5 |
| 4 | The effects of K^+ growth conditions on the accumulation of cesium by the bacterium Thermus sp. TibetanG6显示文摘The accumulation of cesium by the bacterium Thermus sp. TibetanG6 was examined under different K+ growth conditions. The effects of external pH and Na+ on the accumulation of ce-sium were also studied, and the mechanism involved was discussed. K+ regimes played an important role in the accumulation of cesium by the strain TibetanG6. The quantity of cesium accumulated (24 h) was much higher in K+-deficient regime than that in K+-sufficient regime. The pH and Na+ had different effects on the accumulation of cesium in the two K+ regimes. IR spectra analyses indicated that the biosorption is a process of homeostasis with cesium initially accumulated on the cell wall. | WANG Hailei1,2,3, KONG Fanjing1,2,3 & ZHENG Mianping1,2,3 1. R&D Center for Saline Lake and Epithermal Deposits, Chinese Academy of Geological Sciences, Beijing 100037, China 2. Key Open Laboratory of Saline Lake Resources and Environment, Chinese Academy of Geological Sciences, Beijing 100037, China 3. Institute of Mineral Resources, Chinese Academy of Geological Sciences, Beijing 100037, China | 2006 | Science China(Life Sciences)2006，49，2： | 3 |
| 5 | Interaction between HIF-1 alpha (ODD) and hARD1 does not induce acetylation and destabilization of HIF 1 alpha显示文摘 | Arnesen T Kong X Evjenth R | 2005 | FEBS Lett2005，579，28： | 1 |
| 6 | Tribological properties of biomimetic nano- patterned polymeric surfaces on silicon wafer 显示文摘 | Yoon E S Singh R A Kong H | 2006 | Tribology Letter2006，21，： | 1 |
| 7 | Reactive oxygen species:the unavoidable environmental insult? 显示文摘 | Gracy R W Talent J M Kong Y | 1999 | Mutation Research1999，428，12： | 1 |
| 8 | Recent Advances in Visual and Infrared Face Recognition--A Review显示文摘 | Kong Seong G Heo Jingu Abidi B R | 2005 | Computer Vision and Image Understanding2005，97，1： | 1 |
| 9 | Effect of Chinese herbal medicinal ingredients on peripheral lymphocyte proliferation and serum antibody titer after vaccination in chicken 显示文摘 | Kong XF Hu YL Rui R | 2004 | International Immunopharmacology2004，4，7： | 1 |
| 10 | Experimental investigation of a micro-combined cooling, heating and power system driven by a gas engine显示文摘 | KONG X Q WANG R Z WU J Y | 2005 | International Journal of Refrigeration2005，28，7： | 1 |
| 11 | Protective effects of ginsenoside Rd on PC12 cells against hydrogen peroxide 显示文摘 | Ye R Han J Kong X | 2008 | Biol Pharm Bull2008，31，10： | 1 |
| 12 | Apafl is required for mitochondrial pathways of apoptosis and brain development显示文摘 | Yoshida H Kong YY Yoshida R | | 0，，06： | 1 |
| 13 | 查看详情显示文摘 | Kong L B Hou Z L Xie C R | | 0，，： | 1 |
| 14 | Comprehensive analysis of NAC domain transcription factor gene family in populus trichocarpa 显示文摘 | Hu R B Qi G Kong Y Z | | B M C Plant Biology0，10，： | 1 |
| 15 | Association betweenpolymorphism in the insulin receptor-related receptorgene and growth traits in the Pacific oyster Crassostreagigas显示文摘 | Cong R Kong L Yu H | 2014 | Biochemical Systematics and Ecology2014，54，： | 1 |
| 16 | Organic geochemical characterization of Upper Cretaceous oxic oceanic sediments in Tibet, China: A preliminary study显示文摘 | Zou Y R Kong F Peng P A | 2005 | Cretaceous Research2005，26，： | 1 |
| 17 | Acupuncture deqi, from qualitative history to quantitative measurement显示文摘 | Kong J Gollub R Huang T | 2007 | J Altern Complement Med2007，13，10： | 1 |
| 18 | Dam reservoir eutrophication:A simplified technique for a fast diagnosis of envimnmental degradation 显示文摘 | CEBALLOS R S KONG A OLIVERRA J F | 1998 | Water Res1998，32，： | 1 |
| 19 | Hydrogen-mediated C-C bond formation: A broad new concept in catalytic C-C cou- pling显示文摘 | Ngai M Y Kong J R Krische M J | 2007 | Journal of Organic Chemistry2007，72，4： | 1 |
| 20 | Induction of Bax and ac- tivation of caspase during beta-sitosterol-mediated apoptosis in human colon cancer cells显示文摘 | Choi Y H Kong K R Kim Y A | 2003 | Int J Oncul2003，23，： | 1 |
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