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| 1 | 羊膜深低温保存前后相关因子基因表达的稳定性研究显示文摘目的观察目的基因在新鲜和保存羊膜中表达的完整性。方法取新鲜羊膜深低温保存,以FDA/PI和台盼蓝染色确定上皮细胞活性。用real-tim e RT-PCR评估TGF-β1、EGF、IL1β和IL1RA各阶段的表达能力,结果以2△△CT表示。结果深低温保存后,羊膜上皮细胞经染色证实全部死亡,但细胞培养3周后,会有大量的新生细胞游离出来。如羊膜解冻后立即行总RNA提取,在不同阶段保存羊膜中的总RNA无明显减少,且目的基因的表达同新鲜羊膜中的相同(P>0.05)。如解冻半小时后提取总RNA,其量明显减少(P<0.01)。结论传统的羊膜深低温保存法可有效保护基因的完整性,据此我们提出一个羊膜基因保存库的新概念。 | 毕燕龙 荣翱 Karin Kobuch Chris Lohmann | 2006 | 眼科研究2006，24，3： | 2 |
| 2 | Combined use of partially fluorinated alkanes,perfluorocarbon liquids and silicone oil:an experimental study显示文摘 | Hoerauf H Kobuch K Dresp J Menz DH | 2001 | Graefe Arch Clin Exp Ophthalmol2001，239，5： | 1 |
| 3 | Termination of pregnancy for maternal toxoplasmosis显示文摘 | Kobuch WE Bessieres MH | 1994 | Lancet1994，344，： | 1 |
| 4 | Aortic arch surgery using moderate systemic hypothermia and antegrade cerebral perfusion via the right subclavian artery 显示文摘 | Aebert H Reber D Kobuch R | 2001 | Thorac Cardiovasc Surg2001，49，5： | 1 |
| 5 | Epinephrine,but not dexamethasone, induces apoptosis in retinal pigment epithelium cells in vitro:possible implications on the pathogenesis of central serous chorioretinopathy显示文摘 | Sibayan SA Kobuch K Spiegel D | 2000 | Graefes Aroh Clin Exp Ophthalmol2000，，： | 1 |
| 6 | New substances for intraocular tamponades:perfluorocarbon liquids,hydrofluorocarbon liquids and hydrofluorocarbon-oligomers in vitreoretinal surgery显示文摘 | Kobuch K Menz IH Hoerauf H Dresp JH | 2001 | Graefe Arch Clin Exp Ophthalmol2001，239，9： | 1 |
| 7 | Subretinal electrical stimulation of the rabbit retina with acutely implanted electrode arrays 显示文摘 | Gekeler F Kobuch K Schwahn HN Stett A Shinoda K Zrenner E | 2004 | Graefes Arch Clin Exp Ophthalmol2004，242，7： | 1 |
| 8 | Subretinal electrical stimulation of the rabbit retina with acutely implanted electrode arrays显示文摘 | Gekeler F Kobuch K Schwahn HN | 2004 | Graefe''s Arch Clin Exp Ophthalmol2004，242，7： | 1 |
| 9 | Cellular and nerve regeneration within a biosynthetic extracellular matrix for corneal显示文摘 | Li FF Carisson D Lohmann C Suuronen E Vascotto S Kobuch K | 2003 | Transplantation2003，100，15： | 1 |
| 10 | Vibrio metschnikovii,a rare cause of wound infection显示文摘 | LINDE H J KOBUCH R JAYASINGHE S | 2004 | Journal of clinical microbiology2004，42，10： | 1 |
| 11 | Biostability of micro-photodiode arrays for subretinal implantation显示文摘 | Hammerle H Kobuch K Kohler K Nisch W Sachs H Stelzle M | 2002 | Biomaterials2002，23，3： | 1 |
| 12 | Combined use of partially fluorinated alkanes,perfluorocarbon liquids and silicone oil:an experimental study显示文摘 | Hoerauf H Kobuch K Dresp J | 2001 | Graefe''s Arch Clin Exp Ophthalmol2001，239，： | 1 |
| 13 | Aortic arch surgery using moderate systemic hypothermia and antegrade cerebral perfusion via the right subclavian artery显示文摘 | Aebert H Reber D Kobuch R | 2001 | Thorac Cardiovasc Surg2001，49，5： | 1 |
| 14 | Serum levels of adiponectin are associated with diabetic retinopathy and withadiponectin gene muta- tions in Caucasian patients with diabetes mellitus type 2 显示文摘 | Zietz B Buechlar C Kobuch K | 2008 | A Exp Clin Endocrinol Diabetes2008，116，9： | 1 |
| 15 | New substances for intraocular tamponades:perfluorocarbon liquids,hydrofluorocarbon liquids and hydrofluorocarbonoligomers in vitreoretinal surgery显示文摘 | Kobuch K Menz DH Hoerauf H Dresp JH Gabel VP | 2001 | Graefe Arch Clin Exp Ophthalmol2001，239，： | 1 |
| 16 | New substances for intraocular tamponades:perfluorocarbon liquids,hydrofluorocarbon liquids and hydrofluorocarbon-oligomers in vitreoretinal surgery显示文摘 | Kobuch K Menz DH Hoerauf H | 2001 | Graefe''s Arch Clin Exp Ophthalmol2001，239，： | 1 |
| 17 | Serum levels of adiponectin are associated with diabetic retinopathy and with adiponectin gene mutations in Caucasian patients with diabetes mellitus type 2 显示文摘 | Zietz B Buechler C Kobuch K | 2008 | Exp Clin Endocfinol Diabetes2008，116，9： | 1 |
| 18 | Aortic arch surgery using moderate systemic hy- pothermia and ante-grade cerebral per-fusion via the right subclavian artery 显示文摘 | Aebert H ReberD Kobuch R | 2001 | Thorac Cardiovasc Sur2001，49，5： | 1 |
| 19 | Percutaneous transfemoralimplantation of a new,flexible thoracic aorta endoprosthesis using apercutaneous suture system for vascular suture--initial experiences显示文摘 | Manke C Kobuch R Lenhart M | 2001 | Rofo2001，173，5： | 1 |
| 20 | Repeated transchoroidal implantation and explantation of compound subretinal prostheses: an exploratory study in rabbits显示文摘 | Gekeler F Kobuch K Blatsios G | 2010 | Jpn J Ophthalmol2010，54，5： | 1 |
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