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| 1 | The Tomato Hybrid Proline-rich Protein regulates the abscission zone competence to respond to ethylene signals显示文摘The Tomato Hybrid Proline-rich Protein(THyPRP)gene was specifically expressed in the tomato(Solanum lycopersicum)flower abscission zone(FAZ),and its stable antisense silencing under the control of an abscission zone(AZ)-specific promoter,Tomato Abscission Polygalacturonase4,significantly inhibited tomato pedicel abscission following flower removal.For understanding the THyPRP role in regulating pedicel abscission,a transcriptomic analysis of the FAZ of THyPRP-silenced plants was performed,using a newly developed AZ-specific tomato microarray chip.Decreased expression of THyPRP in the silenced plants was already observed before abscission induction,resulting in FAZ-specific altered gene expression of transcription factors,epigenetic modifiers,post-translational regulators,and transporters.Our data demonstrate that the effect of THyPRP silencing on pedicel abscission was not mediated by its effect on auxin balance,but by decreased ethylene biosynthesis and response.Additionally,THyPRP silencing revealed new players,which were demonstrated for the first time to be involved in regulating pedicel abscission processes.These include:gibberellin perception,Ca2+-Calmodulin signaling,Serpins and Small Ubiquitin-related Modifier proteins involved in post-translational modifications,Synthaxin and SNARE-like proteins,which participate in exocytosis,a process necessary for cell separation.These changes,occurring in the silenced plants early after flower removal,inhibited and/or delayed the acquisition of the competence of the FAZ cells to respond to ethylene signaling.Our results suggest that THyPRP acts as a master regulator of flower abscission in tomato,predominantly by playing a role in the regulation of the FAZ cell competence to respond to ethylene signals. | Srivignesh Sundaresan Sonia Philosoph-Hadas Chao Ma Cai-Zhong Jiang Joseph Riov Raja Mugasimangalam Betina Kochanek Shoshana Salim Michael SReid Shimon Meir | 2018 | Horticulture Research2018，5，1： | 2 |
| 2 | Biomarkers of primary and evolving damage in traumatic and ischemic brain injury: diagnosis, prognosis, probing mechanisms, and therapeutic decision making显示文摘 | Kochanek PM Berger RP Bayr H | 2008 | Curt Opin Crit Care2008，14，2： | 1 |
| 3 | Neutrophils do not mediate blood-brain barrier permeability early after controlled cortical impact in rats显示文摘 | Whalen MJ Carlos TM Kochanek PM | 1999 | J Neurotrauma1999，16，7： | 1 |
| 4 | Interlukine-6 interlukine-10 in cerebrospinal fluid after severe traumatic brain injury in children显示文摘 | Kochanek PM Doughty LA | 1997 | J Neurotrauma1997，14，： | 1 |
| 5 | High-capacity adenoviral vectors for gene transfer and somatic gene therapy显示文摘 | Kochanek S | 1999 | Hum Gene Ther1999，10，15： | 1 |
| 6 | Cerebrovascular response in infants and young children following severe traumatic brain injury:a preliminary report显示文摘 | Adelson PD Clyde B Kochanek PM | 1997 | Pediatr Neurosurg1997，26，4： | 1 |
| 7 | Increases in bcl-2 and cleavage of caspasr-1 and caspase-3 in human brain afrer head in jury 显示文摘 | Clark RS Kochanek PM Chen M | 1999 | FASEB J1999，13，3： | 1 |
| 8 | Influence of granulocytopenia on canine cerebral ischemia induced by air embolism显示文摘 | Dutka A J Kochanek PM Hallenbeck JM | 1989 | Stroke1989，，20： | 1 |
| 9 | Stroke risk factors prepare rat brainstem tissues for modified local shwartzman reaction显示文摘 | Dutka AJ Kochanek PM | 1988 | Stroke1988，19，： | 1 |
| 10 | Interleukin-8 is in- creased in cerebrospinal fluid of children with severe head injury显示文摘 | Whalen MJ Carlos TM Kochanek PM | 2000 | Crit Carc Med2000，28，4： | 1 |
| 11 | Promising strategies to minimize secondary brain injury after head trauma显示文摘 | Bayir H Clark RS Kochanek PM | 2003 | Crit Care Med2003，，： | 1 |
| 12 | The potential for bio-mediators and biomarkers in pediatric traumatic brain injury and neurocritical care显示文摘 | Kochanek PM Berger RP Fink EL | 2013 | Front Neurol2013，4，： | 1 |
| 13 | Photother apy and photochemotherapy显示文摘 | Schneider LA Hinrichs R Scharffetter Kochanek K | 2008 | Clin Dermatol2008，26，5： | 1 |
| 14 | Cytokinin but not gibberellin application had major impact on the phenylpropanoid pathway in grape显示文摘Cytokinin and gibberellic acid(GA)are growth regulators used to increase berry size in seedless grapes and it is of interest to understand their effects on the phenylpropanoid pathway and on ripening processes.GA 3 and synthetic cytokinin forchlorfenuron(N-(2-chloro-4-pyridyl)-N′-phenylurea,CPPU)and their combination were applied to 6 mm diameter fruitlets of‘Sable Seedless’,and berries were sampled 51 and 70 days(d)following application.All treatments increased berry size and delayed sugar accumulation and acid degradation with a stronger effect of CPPU.CPPU,but not GA,reduced berry color and the levels of anthocyanins.While CPPU reduced the levels of anthocyanins by more than 50%,the combined treatment of GA+CPPU reduced the levels by about 25%at 51 d.CPPU treatment had minor effects on flavonols content but increased the levels of monomeric flavan-3-ols by more than two-fold.Phloroglucinol analysis using HPLC showed that proanthocyanidin content was significantly increased by CPPU,whereas mean degree of polymerization was reduced from 26 to 19.Volatile analysis by GC-MS showed changes in composition with CPPU or GA treatment with potential impact on flavor.RNA-seq analysis showed that GA had a minor overall effect on the transcriptome whereas CPPU had pronounced effects on gene expression at both 51 and 70 d.Comparing the control and CPPU at similar Brix of ca.19.7°,a reduced expression of stilbene synthases(STSs)including their regulators MYB14 and MYB15,and other phenylpropanoid-related genes was observed in CPPU-treated grapes.Overall,our study shows that CPPU had a major influence on the phenylpropanoid pathway and affected multiple ripening-related processes. | Kamal Tyagi Itay Maoz Bettina Kochanek Noa Sela Larry Lerno Susan EEbeler Amnon Lichter | 2021 | Horticulture Research2021，8，1： | 1 |
| 15 | Promising strategies to minimize secondary brain injury after head trauma 显示文摘 | Bayir HC Kochanek PM | 2003 | Crit Care Med2003，，： | 1 |
| 16 | High-capacity 'gutless' adenoviral vectors 显示文摘 | KOCHANEK S SCHIEDNER G VOLPERS C | 2001 | Curr Opin Mol Ther2001，3，5： | 1 |
| 17 | Reduced frequencies and suppressive function of CD4^+ CD5hi regulatory T cells in patients with chronic lymphocytic leukemia after therapy with fludarabine显示文摘 | Beyer M Kochanek M Darabi K | 2005 | Blood2005，106，6： | 1 |
| 18 | A dual role for poly-ADP-ribosylation in spatial memory acquisition after traumatic brain injury in mice involving NAD + depletion and ribosylation of 14-3-3γ 显示文摘 | Zhang XP Kochanek PM | 2003 | J Neurochem2003，85，3： | 1 |
| 19 | Detection of single-and double-strand DNA breaks after traumatic brain injury in rats: comparison of in situ labeling techniques using DNA polymerase I, the Klenow fragment of DNA polymerase I,and terminal deoxynucleotidyl transferase 显示文摘 | Chen M Kochanek PM | 2001 | J Neurotrauma2001，18，7： | 1 |
| 20 | Reduced frequencies and suppressive function of CD4^+ CD25^hi regulatory T cells in patients with chronic lymphocytic leukemia after therapy with fludarabine显示文摘 | Beyer M Kochanek M Darabi K | 2005 | Blood2005，106，6： | 1 |
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