|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Serum and urine metabolomic fingerprinting in diagnostics of inflammatory bowel diseases显示文摘AIM:To evaluate the utility of serum and urine metabolomic analysis in diagnosing and monitoring of inflammatory bowel diseases(IBD).METHODS:Serum and urine samples were collected from 24 patients with ulcerative colitis(UC),19 patients with the Crohn’s disease(CD)and 17 healthy controls.The activity of UC was assessed with the Simple Clinical Colitis Activity Index,while the activity of CD was determined using the Harvey-Bradshaw Index.The analysis of serum and urine samples was performed using proton nuclear magnetic resonance(NMR)spectroscopy.All spectra were exported to Matlab for preprocessing which resulted in two data matrixes for serum and urine.Prior to the chemometric analysis,both data sets were unit variance scaled.The differences in metabolite fingerprints were assessed using partial least-squaresdiscriminant analysis(PLS-DA).Receiver operating characteristic curves and area under curves were used to evaluate the quality and prediction performance of the obtained PLS-DA models.Metabolites responsible for separation in models were tested using STATISTICA10 with the Mann-Whitney-Wilcoxon test and the Student’s t test(α=0.05).RESULTS:The comparison between the group of patients with active IBD and the group with IBD in remission provided good PLS-DA models(P value 0.002for serum and 0.003 for urine).The metabolites that allowed to distinguish these groups were:N-acetylated compounds and phenylalanine(up-regulated in serum),low-density lipoproteins and very low-density lipoproteins(decreased in serum)as well as glycine(increased in urine)and acetoacetate(decreased in urine).The significant differences in metabolomic profiles were also found between the group of patients with active IBD and healthy control subjects providing the PLS-DA models with a very good separation(P value<0.001 for serum and 0.003 for urine).The metabolites that were found to be the strongest biomarkers included in this case:leucine,isoleucine,3-hydroxybutyric acid,N-acetylated compounds,acetoacetate,glycine,phenylalanine and lactate(increased in serum),creatine,dimethyl sulfone,histidine,choline and its derivatives(decreased in serum),as well as citrate,hippurate,trigonelline,taurine,succinate and 2-hydroxyisobutyrate(decreased in urine).No clear separation in PLS-DA models was found between CD and UC patients based on the analysis of serum and urine samples,although one metabolite(formate)in univariate statistical analysis was significantly lower in serum of patients with active CD,and two metabolites(alanine and N-acetylated compounds)were significantly higher in serum of patients with CD when comparing jointly patients in the remission and active phase of the diseases.Contrary to the results obtained from the serum samples,the analysis of urine samples allowed to distinguish patients with IBD in remission from healthy control subjects.The metabolites of importance included in this case up-regulated acetoacetate and down-regulated citrate,hippurate,taurine,succinate,glycine,alanine and formate.CONCLUSION:NMR-based metabolomic fingerprinting of serum and urine has the potential to be a useful tool in distinguishing patients with active IBD from those in remission. | Tomasz Dawiskiba Stanislaw Deja Agata Mulak Adam Zabek Ewa Jawień Dorota Pawelka Miroslaw Banasik Agnieszka Mastalerz-Migas Waldemar Balcerzak Krzysztof Kaliszewski Jan Skóra Piotr Bar Krzysztof Korta Kornel Pormańczuk Przemyslaw Szyber Adam Litarski Piotr Mlynarz | 2014 | World Journal of Gastroenterology2014，20，1： | 15 |
| 2 | Deformations of surface and rock mass in salt mines of Southern Poland显示文摘 | J .Szewczyk G. Kortas | 2005 | 中国有色金属学会会刊:英文版2005，15，S1： | 2 |
| 3 | Plasma exchange in rapidly progressive renal failure due to multiple myeloma 显示文摘 | Moist L Nesrallah G Kortas C | 1999 | Am J Nephrol1999，19，1： | 1 |
| 4 | Pressurized liquid extraction for the determination of polyphenols in apple显示文摘 | ALONSO-SALCES R M KORTA E BARRANCO A BERRUETA L A GALLO B VICENTE F | 2001 | Journal of Chromatography A2001，933，1： | 1 |
| 5 | Nocturnal but not short hours quotidian hemodialysis requires an elevated dialysate calcium concentration显示文摘 | Al-Hejaili F Kortas C Leitch R | 2003 | J Am Soc Nephrol2003，14，： | 1 |
| 6 | Effect of usual doses of fo late supplementation on elevated plasma homocysteine in hemodialysis patients:no difference between 1 and 5 mg daily显示文摘 | Spence JD Cordy P Kortas C | 1999 | Am J Nephrol1999，19，： | 1 |
| 7 | Multi-objective optimi- zation of a car body structure显示文摘 | Korta J Raniolo R Danti m | 2012 | SAE International Journal of Passenger Cars-Mechanical Systems2012，5，3： | 1 |
| 8 | Mallory-Weiss tear: predisposing factors and predictors of a complicated course显示文摘 | Kortas D | 2001 | Am J of Gastroenterol2001，96，： | 1 |
| 9 | Study of semi- automated solid-phase extraction for the determination of acaricide residues in honey by liquid chromatography显示文摘 | KORTA E BAKKAL1 A BERRUETA L A | 2001 | Journal of Chromatography A2001，930，12： | 1 |
| 10 | Further identification of races of Cladosporium fulvum (Fulvia fulva) on tomato originating from the Netherlands, France and Poland显示文摘 | Lindhout P Korta W | 1989 | Neth J Plant Pathol1989，95，： | 1 |
| 11 | Effect of usual doses of folate supplementation on elevated plasma homocysteine in hemodialysis patients:no difference between 1 and 5 mg daily 显示文摘 | Spence J D Cordy P Kortas C | 1999 | Am J Nephrol1999，19，： | 1 |
| 12 | Study of semi-automated solid-phase extraction for the determination of acaricide residues in honey by liquid chroma-togragry显示文摘 | Korta | 2001 | Journal of chromatography A2001，930，： | 1 |
| 13 | Associa- tion among lung function, exhaled nitric oxide, and the CAN question- naire to assess asthma control in children显示文摘 | Sardon - Prado O Korta - Murua J Valverde - Molina J | 2010 | Pediatr Pulmono12010，45，5： | 1 |
| 14 | Pressurized liquidextraction for the determination of polyphenols in apple显示文摘 | Alonso - Salces R M Korta E Barranco A | 2001 | JoumalChromotography2001，933，12： | 1 |
| 15 | Determination of arnitraz and other acaricide residues in beeswax显示文摘 | KORTA E BAKKALI A BERRUETA L A | 2003 | Analytica Chimica Acta2003，475，： | 1 |
| 16 | Further identification of rac-es of Cladosporium fulvum (Fulvia fulva) on tomato originatingfrom the Netherlands France and Poland显示文摘 | Lindhout P Korta W Cislik M | 1989 | Netherlands Journalof Plant Pathology1989，95，3： | 1 |
| 17 | Modulation of plasma and platelet vasopressin by cardiac function in patients with heart failure显示文摘 | Bichet DG Kortas C Mettauer B | 1956 | Kidney Int1956，29，： | 1 |
| 18 | Pressurized liquid extraction for the determination ofpolyphenols in apple显示文摘 | Alonso-Salces R M Korta E Barranco A | 2001 | Journal of Chromatography A2001，933，1： | 1 |
| 19 | Experimental and numerical study on the effect of humidity-temperature cycling on structural multi-mate- rial adhesive joints显示文摘 | Korta J Mlyniec A Uhl T | 2015 | Composites Part B2015，79，： | 1 |
| 20 | Pressurized liquid extraction for the determination of polyphenols in apple显示文摘 | R.M. Alonso-Salces E. Korta A. Barranco L.A. Berrueta B. Gallo F. Vicente | 2001 | Journal of Chromatography A2001，，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员