|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Lipopolysaccharide decreasing albumin expression in rat hepatocytes显示文摘The relations of the severity of hypoalbu-minemia to morbidity and mortality of patients with critical illness illustrate the need for better understanding of molecular mechanism of hypoalbuminemia. This study was undertaken to investigate the response of albumin synthesis to lipopolysaccharide(LPS)in rat hepatocytes in vitro in early acute phase of sepsis. METHODS:Hepatocytes were cultured at an initial cell density of 1.5×106 cells/well in 3 ml culture medium. There were two groups of samples which received either normal saline or 1 μg/L LPS randomly. Albumin mRNA in hepatocytes was assessed by reverse transcription-poly-merase chain reaction(RT-PCR) and albumin level in the supernatant was measured by ELISA at 0,2,8,12,24 hours after exposure. Meanwhile, the albumin precursor was evaluated at the same time points by flow cytometry. RESULTS:The quantitative changes of mRNA, albumin precursor and its protein were analogous. All of them tended to decline at 12 hours post-treatment and did not decrease significantly until 24 hours after LPS exposure. Meanwhile, albumin mRNA decreased about 30% and the levels of albumin precursor and albumin reduced approximately 50%. CONCLUSIONS: LPS can inhibit albumin synthesis in rat hepatocytes by prevention of albumin transcription. Moreover, the response of hepatic albumin synthesis to LPS changes with the stage of sepsis process. The results show that albumin metabolism in sepsis is a complicated process and further studies are required to understand the molecular mechanism of LPS-induced hypoalbuminemia in sepsis. | Research Institute of General Surgery, Jinling Hospital, Nanjing 210002, China (Wang XY, Li WQ, Li N and Li JS) and Department of Orthopaedics, Zhongda Hospital, Nanjing 210009, China (Lu J) | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 2 |
| 2 | Parallel algorithm for generalized matrix eigen-problem and its application in calculation of crystal electronic structure显示文摘1 Problem and algorithmMANY computing tasks arising from computational physics, chemistry and biology are relatedto solving so-called generalized eigen-decomposition problems. Consider the following general-ized eigen-decomposition problem: | Sun, JC Deng, JX Cao, JW Wang, DS Zhang, WQ Li, J | 1997 | Chinese Science Bulletin1997，42，13： | 2 |
| 3 | Cell biology of IL-7,a key lymphotrophin显示文摘 | Jiang Q Li WQ Aiello FB | 2005 | Cytokine Growth Factor Rev2005，16，45： | 1 |
| 4 | Calmodulin binds to maize lipid transfer protein and modulates its lipids binding ability 显示文摘 | Li CF Xie WQ Bai WY | 2008 | FEBS J2008，275，21： | 1 |
| 5 | Tripterygium wilfordii Hook F extract suppresses proinflammatory cytokine - induced expression of matrix metalloproteinase genes in articular chondroeytes by inhibiting activating protein- 1 and nuclear factor- kappaB activities显示文摘 | Sylvester J Liacini A Li WQ | 2001 | Mol Pharmacol2001，59，5： | 1 |
| 6 | Elevated hemoglobin A2as a marker for (3-thalassemia trait in pregnant women显示文摘 | Ou ZH Li Q Liu WQ | 2011 | The Tohoku Journal of Experimental Medicine?2011，223，3： | 1 |
| 7 | Molecular mechanisms of N-acetylcysteine actions显示文摘 | Zafarullah M Li WQ Sylvester J | 2003 | Cell Mol Life Sci2003，60，： | 1 |
| 8 | Tripterygium wilfordii Hook F extract suppresses proinflammatory cytokine-induced expression of matrix metalloproteinase genes in articular chondrocytes by inhibiting activating protein-1 and nuclear factor-κappaB activities显示文摘 | Sylvester J Liacini A Li WQ | 2001 | Mol Pharmacol2001，59，5： | 1 |
| 9 | Molecular mechanismsof N- acetyl- cysteine action 显示文摘 | Zafarullah M Li WQ Sylvester J | 2003 | CellMol Life Sci2003，60，1： | 1 |
| 10 | lmmediatc postconditioning during reperfusion attenuates intestinal injury 显示文摘 | Liu KX Li YS Huang WQ | 2009 | Intensive Care Med2009，35，5： | 1 |
| 11 | A CLE-WOX signaling module regulates root meristem maintenance and vascular tissue developm- ent in rice 显示文摘 | Chu HW Liang WQ Li J | 2013 | J Exper Botany2013，64，17： | 1 |
| 12 | Experimental model of H2O2-induced oxidative injury in cardiomyocytes 显示文摘 | Li Y Zhang SH Gong WQ Sun B | 2001 | Di-si Junyi Daxue Xuebao (J Fourth Mil Med Univ)2001，22，18： | 1 |
| 13 | Evaluation of nursing quality and evaluation index system 显示文摘 | Ye WQ Li L | 2012 | Shanghai Nursing Journal2012，12，3： | 1 |
| 14 | Effects of aging on arterial distensibility in populations with high and low prevalence of hypertension:comparison between urban and rural communities in China显示文摘 | Avolio AP Deng FQ Li WQ | 1985 | Circulation1985，71，2： | 1 |
| 15 | Molecular mechanisms of N-acetylcysteine actions显示文摘 | Zafarullah M Li WQ Sylvester J | 2003 | Cell Mol Life Sci2003，60，1： | 1 |
| 16 | Association between genetic poly-morphisms of DNA base excision repair genes and evolution of pre-cancerous gastric lesions in a Chinese population显示文摘 | Li WQ Zhang L Ma JL | 2009 | Carcinogene-sis2009，30，3： | 1 |
| 17 | The cardiovascular effects of central hydrogen sulfide are related to K (ATP) channels activation 显示文摘 | Liu WQ Chai C Li XY | 2011 | Physiol Res2011，60，5： | 1 |
| 18 | Profiling membrane lipids in plant stress responses:Role of phospholipase D alpha in freezing-induced lipid changes in Arabidopsis显示文摘 | Ruth W Li WQ Li MM | 2002 | J Biol Chem2002，277，31： | 1 |
| 19 | Mononuclear histocompatibility leukoeyte antigen-DR expression in the early phase of acute pancreatitis 显示文摘 | Yu WK Li WQ Li N | 2004 | Pancreatology2004，4，34： | 1 |
| 20 | Molecular mecha- nisms of N-acetylcysteine actions显示文摘 | Zafarullah M Li WQ Sylvester J | 2003 | Cell Mol Life Sci2003，60，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员