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| 1 | Identification of tumor associated single-chain Fv by panning and screening antibody phage library using tumor cells显示文摘AIM:To study the feasibility of panning and screeningphage-displaying recombinant single-chain variablefragment (ScFv) of anti-tumor monoclonal antibodiesfor fixed whole cells as the carriers of mAb-bindingantigens.ME,ODS: The recombinant phage displaying librariesfor anti-colorectal tumor mAb MC3Ab, MC5Ab andanti-gastric tumor mAb MGD1 was constructed.Panning and screening were carried outby means ofmodified fixation of colorectal and gastric tumor cellsexpressed the mAb-binding antigens. Concordance ofbinding specificity to tumor cells between phageclones and parent antibodies was analyzed. Thephage of positive clones was identified withcompetitive ELISA, and infected by E.coli HB2151 toexpress soluble ScFv.RESULTS:The ratio of positive clones to MC3-ScF-MC5-ScFv and MGD1-ScFv were 60 %, 24 % and 30 %. MC3-ScFv had Mr 32 000 confirmed by Western blot. Thespecificity to antigen had no difference between 4positive recombinant phage antibodies and MC3Ab.CONCLUSION:The modified process of fixing wholetumor cells is efficient, convenient and feasible to panand screen the phage-displaying ScFv of anti-tumormonoclonal antibodies. | Nie, YZ He, FT Li, ZK Wu, KC Cao, YX Chen, BJ Fan, DM | 2002 | World Journal of Gastroenterology2002，8，4： | 12 |
| 2 | 钠-葡萄糖共转运蛋白-2抑制剂或胰高血糖素样肽-1受体激动剂治疗成人2型糖尿病:临床实践指南显示文摘临床问题对于存在不同心血管风险及肾脏结局的2型糖尿病患者,在原有生活方式干预和/或其他降糖药物的基础上加用钠-葡萄糖共转运蛋白2(SGLT-2)抑制剂和胰高血糖素样肽1(GLP-1)受体激动剂的获益及风险是什么?现行做法几十年来,2型糖尿病的治疗决策都以控制血糖为主导。SGLT-2抑制剂和GLP-1受体激动剂在传统观念中常被用于二甲双胍治疗后血糖仍控制不佳的患者。目前这一现状已经发生了改变,这得益于多项临床研究结果。研究显示SGLT-2抑制剂和GLP-1受体激动剂拥有独立于药物降糖作用之外的对于动脉粥样硬化性心血管病(CVD)和慢性肾脏病(CKD)的获益。建议本指南阐述了针对不同风险分层的成人2型糖尿病患者使用SGLT-2抑制剂或GLP-1受体激动剂的建议。•伴有3种或更少的心血管风险因素且不存在CVD或CKD:不建议启动SGLT-2抑制剂或GLP-1受体激动剂治疗。(推荐等级:弱)•伴有3种以上心血管风险因素且不存在CVD或CKD:建议启动SGLT-2抑制剂治疗,不建议启动GLP-1受体激动剂治疗。(推荐等级:弱)•已经存在CVD或CKD:建议启动SGLT-2抑制剂治疗和GLP-1受体激动剂治疗。(推荐等级:弱)•已经存在CVD和CKD:建议启动SGLT-2抑制剂治疗(推荐等级:强)和GLP-1受体激动剂治疗。(推荐等级:弱)•对于那些想要进一步降低CVD和CKD结局风险的患者:推荐优先启用SGLT-2抑制剂治疗而非GLP-1受体激动剂治疗。(推荐等级:弱)这项指南是如何制订的一个由患者、临床医生和方法学家共同组成的国际小组提出了这些推荐意见。这些推荐意见基于可信度较高的指南的标准,并使用GRADE分级方法进行评估。该小组采用了息者个体化的观点。证据一项关于获益与风险的系统综述和网络meta分析(764项随机对照研究,包括421346例参与者)发现SGLT-2抑制剂和GLP-1受体激动剂可以降低总体死亡率、心肌梗死发生率、终末期肾病或肾衰竭的发生率(中等至高等质量的证据)。在不同的亚组中这些药物对卒中、因心力衰竭所致住院和其他主要不良事件有不同的影响。药物绝对获益的程度因患者个体风险的不同有很大的差异。(例如,对于接受了超过5年药物治疗的1000例患者,在最低风险人群中死亡人数减少了5人,在最高风险人群中死亡人数减少了48人)。一项关于预后的综述确认了14种风险预测模型,其中一种(RECODe)在证据总结中报告了大部分基线风险评估数据,小组利用该模型以支持风险分层的建议。考虑到患者的价值观及个体差异,指南推荐的支撑证据包括一项对已发表论文的系统综述、一项患者焦点小组研究、一项临床问题总结,以及一项指南调查。指南解读我们依据不同的CVD和CKD风险水平,综合考虑获益、风险和其他因素的平衡,以及每一个风险组别的实际问题,来对推荐意见进行分层。本指南强烈建议CVD和CKD患者使用SGLT-2抑制剂治疗,这说明专家组认为其具有显著的获益。而对于其他成人2型糖尿病患者,推荐等级较弱,这说明专家组想要在获益、风险及治疗花费上取得一个更好的平衡。临床医生通过该指南可以使用可靠的风险计算模型,如RECODe,来明确其患者的个体心血管和肾脏疾病风险。医患交互式总结临床证据和制订决策有助于患者知晓治疗选择,包括进行共同决策。2型糖尿病人群(全球患病率不断增长1-2)正面临着不断增加的心血管疾病、肾脏病和其他并发症的风险3。数十年来,2型糖尿病的管理始终以控制血糖及糖化血红蛋白(HbA1c)为治疗目标4-5,但是,最近的高质量随机对照研究已经对这种以血糖为中心的治疗模式发起了挑战。研究结果显示,强化血糖控制未必会降低大血管不良事件,它还可能带来不利影响监管机构现在要求新型糖尿病药物必须证明其具有心血管和肾脏获益才能获得批准。对两类新药--钠-葡萄糖共转运蛋白2(SGLT-2)抑制剂和胰高血糖素样肽1(GLP-1)受体激动剂(见框图1)的临床试验结果显示,在现有治疗方案(常规治疗)之上加用这些药物,对死亡、心肌梗死、卒中、心力衰竭和肾脏的结局(如进展为终末期肾病)都有获益8-12。 | Sheyu Li Per Olav Vandvik Lyubov Lytvyn Gordon H Guyatt Suetonia C Palmer Rene Rodriguez-Gutierrez Farid Foroutan Thomas Agoritsas Reed A C Siemieniuk Michael Walsh Lawrie Frere David J Tunnicliffe Evi V Nagler Veena Manja Bjφrn Olav Asvold Vivekanand Jha Mieke Vermandere Karim Gariani Qian Zhao Yan Ren Emma Jane Cartwright Patrick Gee Alan Wickes Linda Fems Robin Wright Ling Li Qiukui Hao Reem A Mustafa 无 郭鹤鸣(译) | 2021 | 英国医学杂志中文版2021，24，9： | 7 |
| 3 | Economic transformation and the gender earnings gap in urban China显示文摘 | Bj?rn Gustafsson Shi Li | 2000 | Journal of Population Economics2000，，2： | 3 |
| 4 | A sensitivity study of the WRF model in offshore wind modeling over the Baltic Sea显示文摘Accurate wind modeling is important for wind resources assessment and wind power forecasting. To improve the WRF model configuration for the offshore wind modeling over the Baltic Sea, this study performed a sensitivity study of the WRF model to multiple model configurations, including domain setup,grid resolution, sea surface temperature, land surface data, and atmosphere-wave coupling. The simulated offshore wind was evaluated against LiDAR observations under different wind directions, atmospheric stabilities, and sea status. Generally, the simulated wind profiles matched observations, despite systematic underestimations. Strengthening the forcing from the reanalysis data through reducing the number of nested domains played the largest role in improving wind modeling. Atmosphere-wave coupling further improved the simulated wind, especially under the growing and mature sea conditions.Increasing the vertical resolution, and updating the sea surface temperature and the land surface information only had a slight impact, mainly visible during very stable conditions. Increasing the horizontal resolution also only had a slight impact, most visible during unstable conditions. Our study can help to improve the wind resources assessment and wind power forecasting over the Baltic Sea. | Huidong Li Björn Claremar Lichuan Wu Christoffer Hallgren Heiner Körnich Stefan Ivanell Erik Sahlée | 2021 | Geoscience Frontiers2021，12，6： | 2 |
| 5 | Modulation of pre-mRNA splicing and protein production of fibronectin by TGF-beta 2 in porcine trabecular cells显示文摘 | Li J Tripathi BJ Tripathi RC | 2000 | Invest Ophthalmol Vis Sci2000，41，11： | 2 |
| 6 | Direct evidence of Parkinson pathology spread from the gastrointestinal tract to the brain in rats显示文摘 | Staffan Holmqvist Oldriska Chutna Luc Bousset Patrick Aldrin-Kirk Wen Li Tomas Bj?rklund Zhan-You Wang Laurent Roybon Ronald Melki Jia-Yi Li | 2014 | Acta Neuropathologica2014，，6： | 2 |
| 7 | Radiation activates HIF - 1 to regulate vascular radiosensitivity in tumors: role of reoxygenation, free radicals, and stress granules 显示文摘 | Moeller BJ Cao Y Li CY | 2004 | Cancer Cell2004，4，： | 1 |
| 8 | Brain metabolite profiles of T1-hypointense lesions in relapsing-remitting multiple sclerosis显示文摘 | Li BS Regal J Soher BJ | 2003 | AJNR2003，24，： | 1 |
| 9 | A novel role for vitamin D: modulation of expression and function of the local renin-angiotensin system in mouse pancreatic islets 显示文摘 | Cheng Q Li YC Boucher BJ | 2011 | Diabetologia2011，54，8： | 1 |
| 10 | Pharmacokinetics of active metabolite of pmlifloxacin in healthy chinese显示文摘 | Wei CM Wang BJ Li HY | 2005 | J Chin Pharma Sci2005，14，4： | 1 |
| 11 | Economic transformation and the gender earnings gap in urban China显示文摘 | Bj?rn Gustafsson Shi Li | 2000 | Journal of Population Economics2000，，2： | 1 |
| 12 | Low-level vagosympathetic nerve stimulation inhibits atrial fibrillation inducibility: direct evidence by neural recordings from intrinsic cardiac ganglia 显示文摘 | Yu L Scherlag BJ Li S | 2011 | J Cardiovasc Electrophysiol2011，2，4： | 1 |
| 13 | Kinetic analysis on the two-step processes of AOB and NOB in aerobic nitri- fying granules显示文摘 | Fang F Ni BJ Li XY | 2009 | Applied Microbiology and Bioteeh- nology2009，83，6： | 1 |
| 14 | Modulation of pre-mRNA splicing and protein production of fibronectin by TGFbeta 2 in porcine trabecular cells显示文摘 | Li J Tripathi BJ Tripathi RC | 2000 | Invest Ophthalmol Vis Sci2000，，： | 1 |
| 15 | Radiation activates HIF-1 to regu-late vascular radiosensitivity in tumors:role of reoxygenation,freeradicals,and stress granules显示文摘 | Moeller BJ Cao Y LI CY | 2004 | Cancer Cell2004，5，5： | 1 |
| 16 | Trabecular cells of the eye express messenger RNA for transforming growth factor-beta 1 and secrete this cytokine显示文摘 | Tripathi RC Li J Borisuth NS Tripathi BJ | 1993 | Invest Ophthalmol Vis Sci1993，34，8： | 1 |
| 17 | Economic transformation and the gender earnings gap in urban China显示文摘 | Bj?rn Gustafsson Shi Li | 2000 | Journal of Population Economics2000，，2： | 1 |
| 18 | Bone morphogenetic protein type II receptor mutations causing protein misfolding in heritable pulmonary arterial hypertensio~ 显示文摘 | Li W Dunmore BJ Morrell NW | 2010 | Proe Am Thorac Soc2010，7，6： | 1 |
| 19 | Economic transformation and the gender earnings gap in urban China显示文摘 | Bj?rn Gustafsson Shi Li | 2000 | Journal of Population Economics2000，，2： | 1 |
| 20 | Radiation activates HIF-1 to regulate vascular radiosensitivity in tumors:role of reoxygenation free radicals and stress granules显示文摘 | Moeller BJ Cao Y Li CY | 2004 | Cancer Cell2004，5，5： | 1 |
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