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| 1 | High-throughput screening identifies established drugs as SARS-CoV-2 PLpro inhibitors显示文摘A new coronavirus(SARS-CoV-2)has been identified as the etiologic agent for the COVID-19 outbreak.Currently,effective treatment options remain very limited for this disease;therefore,there is an urgent need to identify new anti-COVID-19 agents.In this study,we screened over 6,000 compounds that included approved drugs,drug candidates in clinical trials,and pharmacologically active compounds to identify leads that target the SARS-CoV-2 papain-like protease(PLpro).Together with main protease(Mpro),PLpro is responsible for processing the viral replicase polyprotein into functional units.There-fore,it is an attractive target for antiviral drug develop-ment.Here we discovered four compounds,YM155,cryptotanshinone,tanshinone I and GRL0617 that inhibit SARS-CoV-2 PLpro with IC50 values ranging from 1.39 to 5.63 pmol/L.These compounds also exhibit strong antiviral activities in cell-based assays.YM155,an anti-cancer drug candidate in clinical trials,has the most potent antiviral activity with an EC50 value of 170 nmol/L.In addition,we have determined the crystal structures of this enzyme and its complex with YM155,revealing a unique binding mode.YM155 simultaneously targets three'hot'spots on PLpro,including the substrate-binding pocket,the interferon stimulating gene product 15(ISG15)binding site and zinc finger motif.Our results demonstrate the efficacy of this screening and repur-posing strategy,which has led to the discovery of new drug leads with clinical potential for COVID-19 treatments. | Yao Zhao Xiaoyu Du Yinkai Duan Xiaoyan Pan Yifang Sun Tian You Lin Han Zhenming Jin Weijuan Shang Jing Yu Hangtian Guo Qianying Liu Yan Wu Chao Peng Jun Wang Chenghao Zhu Xiuna Yang Kailin Yang Ying Lei Luke W.Guddat Wenqing Xu Gengfu Xiao Lei Sun Leike Zhang Zihe Rao Haitao Yang | 2021 | Protein & Cell2021，12，11： | 4 |
| 2 | An efficient semi-blind source extraction algorithm and its applications to biomedical signal extraction显示文摘In many applications, such as biomedical engineering, it is often required to extract a desired signal instead of all source signals. This can be achieved by blind source extraction (BSE) or semi-blind source extraction, which is a powerful technique emerging from the neural network field. In this paper, we propose an efficient semi-blind source extraction algorithm to extract a desired source signal as its first output signal by using a priori information about its kurtosis range. The algorithm is robust to outliers and spiky noise because of adopting a classical robust contrast function. And it is also robust to the estimation errors of the kurtosis range of the desired signal providing the estimation errors are not large. The algorithm has good extraction performance, even in some poor situations when the kurtosis values of some source signals are very close to each other. Its convergence stability and robustness are theoretically analyzed. Simulations and experiments on artificial generated data and real-world data have confirmed these results. | YE YaLan SHEU Phillip C-Y ZENG JiaZhi WANG Gang LUKe | 2009 | Science in China(Series F)2009，52，10： | 3 |
| 3 | Housing Sulfur in Polymer Composite Frameworks for Li–S Batteries显示文摘Extensive efforts have been devoted to the design of micro-, nano-, and/or molecular structures of sulfur hosts to address the challenges of lithium–sulfur(Li–S) batteries, yet comparatively little research has been carried out on the binders in Li–S batteries. Herein, we systematically review the polymer composite frameworks that confine the sulfur within the sulfur electrode, taking the roles of sulfur hosts and functions of binders into consideration. In particular, we investigate the binding mechanism between the binder and sulfur host(such as mechanical interlocking and interfacial interactions), the chemical interactions between the polymer binder and sulfur(such as covalent bonding, electrostatic bonding, etc.), as well as the beneficial functions that polymer binders can impart on Li–S cathodes, such as conductive binders, electrolyte intake, adhesion strength etc. This work could provide a more comprehensive strategy in designing sulfur electrodes for long-life, large-capacity and high-rate Li–S battery. | Luke Hencz Hao Chen Han Yeu Ling Yazhou Wang Chao Lai Huijun Zhao Shanqing Zhang | 2019 | Nano-Micro Letters2019，11，1： | 2 |
| 4 | A Dairy Industry Information Cooperative Service System Based on a Production Process Ontology显示文摘Agricultural information cooperative services(AICS) are now becoming an important aspect of agriculture informatization.This study offers an ontological conception of the agricultural production process,establishing operational divisions corresponding to both the stages and the fundamental information requirements of the dairy industry production process in China,yielding a process ontology for the dairy industry as well as a business chain model.A framework for a dairy industry information cooperative service system was established,with service functions realized in a prototype system.The resulting agricultural process ontology built on the basis of biological characteristics has advantages as a classification standard for agricultural information;whereas the business chain model based on this agricultural process ontology allows for an effective distribution of agricultural information cooperative services.This study outlines a concept and demonstrates a prototype of a cooperative service capable of integrating diverse online agricultural information relevant to the dairy industry in China. | XU Yong NIU Fang-qu Luke Bergmann WANG Zhi-qiang WANG Jian | 2012 | Journal of Integrative Agriculture2012，11，5： | 2 |
| 5 | Structural insights into substrate recognition by the type Ⅶ secretion system显示文摘Type VII secretion systems(T7SSs)are found in many disease related bacteria including Mycobacterium tuberculosis(Mtb).ESX-1[early secreted antigen 6 kilodaltons(ESAT-6)system 1]is one of the five subtypes(ESX-1?5)of T7SSs in Mfb,where it delivers virulence factors into host macrophages during infection.However,little is known about the molecular details as to how this occurs.Here,we provide high-resolution crystal structures of the C-terminal ATPase3 domains of EccC subunits from four different Mtb T7SS subtypes.These structures adopt a classic RecA-like a/p fold with a conserved Mg-ATP binding site.The structure of EccCbl in complex with the C-terminal peptide of EsxB identifies the location of substrate recognition site and shows how the specific signaling module XxxxMxF'for Mtb ESX-1 binds to this site resulting in a translation of the bulge loop.A comparison of all the ATPase3 structures shows there are significant differences in the shape and composition of the signal recognition pockets across the family,suggesting that distinct signaling sequences of substrates are required to be specifically recognized by different T7SSs.A hexameric model of the EccC-ATPase3 is proposed and shows the recognition pocket is located near the central substrate translocation channel.The diameter of the channel is?25-A,with a size that would allow helix-bundle shaped substrate proteins to bind and pass through.Thus,our work provides new molecular insights into substrate recognition for Mtb T7SS subtypes and also a possible transportation mechanism for substrate and/or virulence factor secretion. | Shuhui Wang Kaixuan Zhou Xiaolin Yang Bing Zhang Yao Zhao Yu Xiao Xiuna Yang Haitao Yang Luke WGuddat Jun Li Zihe Rao | 2020 | Protein & Cell2020，11，2： | 2 |
| 6 | Preparation and Characterization of MWCNTs/E44显示文摘环氧树脂被多围的碳 nanotubes (MWCNT ) 修改与一在原处方法。合成用扫描电子显微镜学(SEM ) 被观察, X-raydiffraction (XRD ) ,和它的磨料穿抵抗,张力的性质被测试。基于 MWCNT 的表面的功能组帮助改进树脂的裂缝抵抗的结果表演,和裂缝被发现显著地减少了在合成。尽管树脂 andMWCNTs 身体上被结合,张力的性质并且穿抵抗合成当 MWCNT 的最佳数量被填时,显著地被改进 Epikote-44 (E44 ) 。当 5 wt % 酸化时, MWCNT 被填 E44,有弹性的模量,断裂强度并且穿合成增加的抵抗到 226.7% , 303.3% 和 272.55%(153.29%) 分别地。 | Yan GAO Zhenjia WANG Rui WANG Ji LIANG Luke WANG | 2006 | Journal of Materials Science & Technology2006，22，1： | 1 |
| 7 | Cities evolution tree and applications to predicting urban growth显示文摘 | Jin-Feng Wang Xu-Hua Liu Ling Peng Hong-Yan Chen Luke Driskell Xiao-Ying Zheng | 2012 | Population and Environment2012，，2： | 1 |
| 8 | Porous Orbital Implants, Wraps, and Peg Placement in the Pediatric Population After Enucleation显示文摘 | Jia-Kang Wang Shu Lang Liao Luke L.K. Lin Shine C.S. Kao Hsiao-Sen Tseng | 2007 | American Journal of Ophthalmology2007，，1： | 1 |
| 9 | A Retrospective Study of Intraoperative Awareness with Methodological Implications显示文摘 | George A. Mashour Luke Y.-J. Wang Christopher R. Turner John C. Vandervest Amy Shanks Kevin K. Tremper | 2009 | Anesthesia & Analgesia2009，，2： | 1 |
| 10 | Value of Contrast-enhanced Ultrasound in Evaluating Synovitis and Predicting Recurrent Joint Bleeding of Hemophilia显示文摘Objective Contrast-enhanced ultrasound(CEUS)is advantageous for evaluating microcirculation,and has been applied to assess arthritis in previous studies.However,CEUS examinations have not been studied for hemophilia arthritis.Hemophilia arthritis is different from other arthritis,because it is induced by spontaneous joint bleeding.Hence,CEUS may have special value in evaluating hemophilia arthritis.The present study assessed the value of CEUS in evaluating synovial hypertrophy and predicting recurrent joint bleeding in severe hemophilia A patients.Methods From August 2016 to January 2017,81 severe hemophilia A patients,who were referred to our hospital for ultrasound joint assessment with conventional ultrasound,were enrolled.Among these 81 patients,46 patients consented for CEUS examinations on the same day.Results Compared to color Doppler flow imaging(CDFI),four more joints presented with a blood flow signal under CEUS mode.In addition,the synovial hypertrophy measured by CEUS was thicker than that measured by conventional ultrasound.The ultrasound scores(including the total grey-scale ultrasound score,joint effusion/hemarthrosis,synovial hypertrophy,CDFI semi-quantitative score,and CEUS semi-quantitative score)were significantly higher in the joint bleeding group than in the no joint bleeding group(P<0.05).Furthermore,these ultrasound scores were positively correlated with the joint bleeding frequency,and had the highest correlation with the CEUS score(r=0.620,P<0.05).Conclusion CEUS can more accurately assess the degree of synovial hypertrophy and vascularization,and diagnose synovitis,when compared to conventional ultrasound.In addition,CEUS appears to be essential for evaluating the possibility of recurrent joint bleeding,and providing more reliable evidence for individualized treatment. | Fei MA Ya-ru ZHANG Koon Hung LUKE Hao LIU Wan-xian LUO Li ZHANG Li-ling XIAO Mei YI Yang GAO Wei-zhen WANG Shi-qiu QIU Xiao-qin FENG Jing SUN Ying-jia LI | 2022 | Current Medical Science2022，42，2： | 1 |
| 11 | Chromizing behaviors of a low carbon steel processed by means of surface mechanical attrition treatment 显示文摘 | WANG Zhen-bo LU Jian LUKe | 2005 | Acta Mater2005，53，7： | 1 |
| 12 | An evaluation of two inexpensive energy-balance techniques for measuring water use in wood-irrigated pecans (Carya illinoinensis)显示文摘 | Luke J Simmons Junming Wang Ted W | 2007 | agricultural water management2007，88，： | 1 |
| 13 | Expression of hexokinase isoforms in the dorsal root ganglion of the adult rat and effect of experimental diabetes 显示文摘 | Gardiner N J Wang Z C Luke C | 2007 | Brain Res2007，1175，： | 1 |
| 14 | Robust object removal with an exemplar-based image inpainting approach显示文摘 | WANG Jing LUKe PAN Daru | 2014 | Neurocomputing2014，123，10： | 1 |
| 15 | Expression of hexokinase isoforms in the dorsal root ganglion of the adult rat and effect of experimental diabetes 显示文摘 | Gardiner NJ Wang Z Luke C | 2007 | Brain Res2007，1175，： | 1 |
| 16 | Multifunctional Epidermal Electronics Printed Directly Onto the Skin显示文摘 | Woon‐Hong Yeo Yun‐Soung Kim Jongwoo Lee Abid Ameen Luke Shi Ming Li Shuodao Wang Rui Ma Sung Hun Jin Zhan Kang Yonggang Huang John A. Rogers | 2013 | Mater2013，，20： | 1 |
| 17 | Kernel optimization for blind motion det)hn'ring with image edge prior显示文摘 | WANG Jing LUKe WANG Qian | 2012 | Mathematical Problems in Engineering2012，2012，63： | 1 |
| 18 | The impact of ischemic cholangiopathy in liver transplantation using donors after cardiac death: The untold story显示文摘 | Anton I. Skaro Colleen L. Jay Talia B. Baker Edward Wang Sarina Pasricha Vadim Lyuksemburg John A. Martin Joseph M. Feinglass Luke B. Preczewski Michael M. Abecassis | 2009 | Surgery2009，，4： | 1 |
| 19 | Structure-activity relationships for a new family of sulfonylurea herbicides显示文摘 | Jian-Guo Wang Zheng-Ming Li Ning Ma Bao-Lei Wang Lin Jiang Siew Siew Pang Yu-Ting Lee Luke W. Guddat Ronald G. Duggleby | 2005 | Journal of Computer - Aided Molecular Design2005，，11： | 1 |
| 20 | Phylogeny,molecular evolution,and dating of divergences in Lagerstroemia using plastome sequences显示文摘Lagerstroemia L.(Lythraceae)is a widely distributed genus of trees and shrubs native to tropical and subtropical environments from Southeast Asia to Australia,with numerous species highly valued as ornamentals.Although the plastomes of many species in this genus have been sequenced,the rates of functional gene evolution and their effect on phylogenetic analyses have not been thoroughly examined.We compared three plastome sequence matrices to elucidate how differences in these datasets affected phylogenetic analyses.Robust phylogenetic relationships for Lagerstroemia species were reconstructed based on different plastome sequence partitions and multiple phylogenetic methods.Identification of single-nucleotide variants within different genes also provides basic data on the patterns of functional gene evolution in Lagerstroemia and may provide insights into how those mutations affect protein structure and potentially drive divergence via cytonuclear incompatibility.These results as well as analyses of non-synonymous and synonymous mutations,indicate that heterotachic modes of evolution are present in functional plastome genes and should be accounted for in the analyses of molecular evolution.In addition,divergence events within the Lagerstroemia were dated for the first time.Several of the divergence estimates corresponded to well-known Earth history events,such as the reduction in global temperatures at the Eocene/Oligocene boundary.Our analyses conducted in Lagerstroemia here dissects the various patterns in the divergence of Lagerstroemia and may provide a useful guide to help plant breeders,as well as the necessity of using plastomic data and as possible as to combine evidence from morphological characteristics to investigate the complicated interspecies relationship and the evolutionary dynamics of species. | Jie Wang Wenchuang He Xuezhu Liao Jin Ma Wei Gao Haoqi Wang Dili Wu Luke R.Tembrock Zhiqiang Wu Cuihua Gu | 2023 | Horticultural Plant Journal2023，9，2： | 1 |
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