|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Helicobacter pylori infection: Host immune response, implications on gene expression and microRNAs显示文摘Helicobacter pylori(H. pylori) infection is the most common bacterial infection worldwide. Persistent infection of the gastric mucosa leads to inflammatory processes and may remain silent for decades or progress causing more severe diseases, such as gastric adenocarcinoma. The clinical consequences of H. pylori infection are determined by multiple factors, including host genetic predisposition, gene regulation, environmental factors and heterogeneity of H. pylori virulence factors. After decades of studies of this successful relationship between pathogen and human host, various mechanisms have been elucidated. In this review, we have made an introduction on H. pylori infection and its virulence factors, and focused mainly on modulation of host immune response triggered by bacteria, changes in the pattern of gene expression in H. pylori-infected gastric mucosa, with activation of gene transcription involved in defense mechanisms, inflammatory and immunological response, cell proliferation and apoptosis. We also highlighted the role of bacteria eradication on gene expression levels. In addition, we addressed the recent involvement of different microRNAs in precancerous lesions, gastric cancer, and inflammatory processes induced by bacteria. New discoveries in this field may allow a better understanding of the role of major factors involved in the pathogenic mechanisms of H. pylori. | Aline Cristina Targa Cadamuro Ana Flávia Teixeira Rossi Nathália Maciel Maniezzo Ana Elizabete Silva | 2014 | World Journal of Gastroenterology2014，20，6： | 16 |
| 2 | MGMT and MLH1 methylation in Helicobacter pylori-infected children and adults显示文摘AIM:To evaluate the association between Helicobacter pylori(H.pylori) infection and MLH1 and MGMT methylation and its relationship with microsatellite instability(MSI).METHODS:The methylation status of the MLH1 and MGMT promoter region was analysed by methylation specific methylation-polymerase chain reaction(MSPPCR) in gastric biopsy samples from uninfected or H.pylori-infected children(n = 50),from adults with chronic gastritis(n = 97) and from adults with gastric cancer(n = 92).MLH1 and MGMT mRNA expression were measured by real-time PCR and normalised to a constitutive gene(β actin).MSI analysis was performed by screening MSI markers at 4 loci(Bat-25,Bat-26,D17S250 and D2S123) with PCR;PCR products were analysed by single strand conformation polymorphism followed by silver staining.Statistical analyses were performed with either the χ 2 test with Yates continuity correction or Fisher's exact test,and statistical significance for expression analysis was assessed using an unpaired Student's t-test.RESULTS:Methylation was not detected in the promoter regions of MLH1 and MGMT in gastric biopsy samples from children,regardless of H.pylori infection status.The MGMT promoter was methylated in 51% of chronic gastritis adult patients and was associated with H.pylori infection(P < 0.05);this region was methylated in 66% of gastric cancer patients,and the difference in the percentage of methylated samples between these patients and those from H.pylori-infected chronic gastritis patients was statistically significant(P < 0.05).MLH1 methylation frequencies among H.pylori-infected and non-infected chronic gastritis adult patients were 13% and 7%,respectively.We observed methylation of the MLH1 promoter(39%) and increased MSI levels(68%) in samples from gastric cancer patients in comparison to samples from H.pylori-infected adult chronic gastritis patients(P < 0.001 and P < 0.01,respectively).The frequency of promoter methylation for both genes was higher in gastric cancer samples than in H.pylori-positive chronic gastritis samples(P < 0.05).The levels of MLH1 and MGMT mRNA were significantly reduced in chronic gastritis samples that were also hypermethylated(P < 0.01).CONCLUSION:In summary,MGMT and MLH1 methylation did not occur in earlier-stage H.pylori infections and thus might depend on the duration of infection. | Marisa C Alvarez Juliana C Santos Nathália Maniezzo Marcelo S Ladeira Artur LC da Silva Isabel CA Scaletsky José Pedrazzoli Jr Marcelo L Ribeiro | 2013 | World Journal of Gastroenterology2013，19，20： | 5 |
| 3 | The ant system: Optimization by a colony of cooperating agents显示文摘 | Dorigo M Maniezzo V Colorni A | 1996 | IEEE Transactions on Systems Man and Cybemetics-Part B1996，26，1： | 2 |
| 4 | Ant System: Optimization by A Colony of Cooperating Agents 显示文摘 | Dorigo M Maniezzo V Colorni A | 1996 | IEEE Transactions on Systems Man and Cybernetics Part B1996，26，1： | 2 |
| 5 | The ant system: optimization by a colony of cooperating agents 显示文摘 | Dorigo M Maniezzo V Colomi A | 1996 | IEEE Transactions on Systems Man & Cybernetics B1996，26，2： | 2 |
| 6 | Ant system:optimization by a colony of cooperating agents显示文摘 | Dorigo M Maniezzo V Colorni A | 1996 | IEEE Transactions on SMC1996，26，1： | 1 |
| 7 | The ant system: optimization by a colony of cooperative learning approach to the traveling agents 显示文摘 | Dorigo M Maniezzo V Colorni A | 1996 | IEEE Transactions on Systems Man and Cybernetics1996，26，1： | 1 |
| 8 | The ant system: Optimization by a colony of cooperating agents 显示文摘 | Dorigo M Maniezzo V Colorni A | 1996 | IEEE Transactions on Systems Man and Cybernetics1996，26，1： | 1 |
| 9 | The ant system:optimization by a colony of cooperating agents显示文摘 | Dorigo M Maniezzo V Colorni A | 1996 | IEEE Trans Syst Man Cybern Part B1996，26，1： | 1 |
| 10 | Ant system:optimization by a colony of cooperating agents显示文摘 | Maniezzo V Colorni A | 1996 | IEEE Transactions on Systems Man and Cybernetics1996，26，1： | 1 |
| 11 | The Ant System: Optimization by a Colony of Cooperating Agents显示文摘 | Dorigo M Maniezzo V Colorni A | 1996 | IEEE Trans on Systems Man and Cybernetics-Part B1996，26，3： | 1 |
| 12 | Ant system:optimization by a colony of cooperating agents显示文摘 | Dorigo M Maniezzo V Colorni A | 1996 | IEEE Trans on Systems Man and Cybernetics1996，26，1： | 1 |
| 13 | The ant system: Optimization by a colony of cooperating agents显示文摘 | Dorigo M Maniezzo V Colorni A | 1996 | IEEE Transactions on Systems Man and Cybernetics-Part B1996，26，1： | 1 |
| 14 | The Ant System:Optimization by A Colony of Cooperating Angents显示文摘 | Dorigo M Maniezzo V Colomi A | 1996 | IEEE Transactions on Systems Man & Cybernetics B1996，26，2： | 1 |
| 15 | Ant System for Job -Shop Scheduling 显示文摘 | Colorni A Dorigo M Maniezzo V | 1994 | Belgian Journal of Operations Research and Statistic Computing Science1994，34，1： | 1 |
| 16 | Ant system: Optimization by a colony of cooperating agents显示文摘 | Dorigo M Maniezzo V Colorni A | 1996 | IEEE Transactions on Systems Man and Cybernetics Part B: Cybernetics1996，26，： | 1 |
| 17 | Ant system:optimization by a colony of cooperating agents显示文摘 | DORIGO M MANIEZZO V COLORNI A | | 0，，01： | 1 |
| 18 | The ant system : optimization by a colony of cooperating Agents 显示文摘 | Dorigo M Maniezzo V Colorni A | 1996 | IEEE Transactions on Systems1996，26，1： | 1 |
| 19 | The Ant System:Optimization by a colony of cooperating agents显示文摘 | Dorigo M Maniezzo V Colorni A | 1996 | IEEE Transactions on Systems Man and Cybernetics Part B1996，26，1： | 1 |
| 20 | Ant System: Optimization by a Colony of Cooperating Agent 显示文摘 | Dorigo M Maniezzo V Colorni A | 1996 | IEEE Transactions on Systems Man and Cybernetics-Part B (S1083-4419)1996，26，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员