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| 1 | 胎盘生长因子和色素上皮衍生因子在非小细胞肺癌中的表达及其与预后的关系显示文摘目的探讨胎盘生长因子(PlGF)和色素上皮衍生因子(PEDF)在非小细胞肺癌中的表达特点及与肿瘤新生血管形成的关系,了解这两种因子在判断非小细胞肺癌患者预后中的意义。方法采用超敏过氧化酶免疫组织化学法检测手术切除的81例非小细胞肺癌标本中PlGF和PEDF的表达水平,并利用CD31染色进行微血管计数,统计分析这两个因子与微血管密度(MVD)的关系,及其在非小细胞肺癌患者预后中的意义。结果PlGF和PEDF在81例非小细胞肺癌患者中的阳性率分别为43·2%(35例)和53·1%(43例)。PlGF阳性表达与高值MVD相关;PEDF阳性表达与低值MVD相关;PlGF和PEDF的表达状况存在负相关。单因素和多因素分析均提示PlGF是非小细胞癌患者独立的预后指标。结论PlGF和PEDF均可在非小细胞肺癌组织中表达,二者在血管生成的过程中具有相互拮抗的作用。与PEDF相比,PlGF在非小细胞肺癌发展以及预测患者预后方面具有更大的作用。 | 张力建 陈晋峰 陆爱萍 柯杨 Robert E Mansel Wen G Jiang | 2005 | 中华医学杂志2005，85，47： | 10 |
| 2 | Effects of intermittent fasting on health markers in those with type 2 diabetes:A pilot study显示文摘AIM To determine the short-term biochemical effects and clinical tolerability of intermittent fasting(IF) in adults with type 2 diabetes mellitus(T2DM).METHODS We describe a three-phase observational study(baseline 2 wk, intervention 2 wk, follow-up 2 wk) designed to determine the clinical, biochemical, and tolerability of IF in community-dwelling volunteer adults with T2DM. Biochemical, anthropometric, and physical activity measurements(using the Yale Physical Activity Survey) were taken at the end of each phase. Participants reported morning, afternoon and evening self-monitored blood glucose(SMBG) and fasting duration on a daily basis throughout all study stages, in addition to completing a remote food photography diary three times within each study phase. Fasting blood samples were collected on the final days of each study phase. RESULTS At baseline, the ten participants had a confirmed diagnosis of T2DM and were all taking metformin, and on average were obese [mean body mass index(BMI) 36.90 kg/m^2]. We report here that a short-term periodof IF in a small group of individuals with T2DM led to significant group decreases in weight(-1.395 kg, P = 0.009), BMI(-0.517, P = 0.013), and at-target morning glucose(SMBG). Although not a study requirement, all participants preferentially chose eating hours starting in the midafternoon. There was a significant increase(P < 0.001) in daily hours fasted in the IF phase(+5.22 h), although few attained the 18-20 h fasting goal(mean 16.82 ± 1.18). The increased fasting duration improved at-goal(< 7.0 mmol/L) morning SMBG to 34.1%, from a baseline of 13.8%. Ordinal Logistic Regression models revealed a positive relationship between the increase in hours fasted and fasting glucose reaching target values(χ~2 likelihood ratio = 8.36, P = 0.004) but not for afternoon or evening SMBG(all P > 0.1). Postprandial SMBGs were also improved during the IF phase, with 60.5% readings below 9.05 mmol/L, compared to 52.6% at baseline, and with less glucose variation. Neither insulin resistance(HOMAIR), nor inflammatory markers(C-reactive protein) normalized during the IF phase. IF led to an overall spontaneous decrease in caloric intake as measured by food photography(Remote Food Photography Method). The data demonstrated discernable trends during IF for lower energy, carbohydrate, and fat intake when compared to baseline. Physical activity, collected by a standardized measurement tool(Yale Physical Activity Survey), increased during the intervention phase and subsequently decreased in the follow-up phase. IF was well tolerated in the majority of individuals with 6/10 participants stating they would continue with the IF regimen after the completion of the study, in a full or modified capacity(i.e., every other day or reduced fasting hours).CONCLUSION The results from this pilot study indicate that shortterm daily IF may be a safe, tolerable, dietary intervention in T2DM patients that may improve key outcomes including body weight, fasting glucose and postprandial variability. These findings should be viewed as exploratory, and a larger, longer study is necessary to corroborate these findings. | Terra G Arnason Matthew W Bowen Kerry D Mansell | 2017 | World Journal of Diabetes2017，8，4： | 7 |
| 3 | 荧光实时定量PCR检测NSCLC中PEDF基因表达状态的研究显示文摘背景与目的SYBRGreenⅠ是一种较为常用的非探针类定量PCR的方法,其主要优点在于操作过程简单,结果具有一定特异性。本研究的目的是建立检测色素上皮衍生因子(pigmentepitheliumderivedfactor,PEDF)mRNA的SYBRGreenⅠ实时定量PCR的方法,了解肺癌组织及其远癌肺组织中PEDF的表达水平,研究肺癌组织中PEDF的表达水平与各种临床病理特征之间的关系。方法利用RTPCR的方法扩增并纯化PEDF以及内对照GAPDHmRNA的目的片段,倍比稀释后作为定量模板。利用相对定量的方法,检测21例肺癌及相应远癌肺组织中PEDFmRNA相对于GAPDHmRNA的表达量,进行相对定量分析。结果21例肺癌组织及相应的远癌肺组织中均可检出PEDF的表达,肺癌组织中PEDF的相对表达量为0.5505±0.3590(0.11~1.11),远癌肺组织中为0.7219±0.2582(0.29~1.31)(P=0.024)。早期(Ⅰ~Ⅱ期)肺癌患者和肿瘤较小时(T1)PEDF的表达量显著高于晚期(Ⅲ~Ⅳ期,P=0.010)和肿瘤较大者(T2~4,P=0.007)。结论所建立的SYBRGreenⅠ实时定量PCR方法可以成功地检测PEDF基因的表达量。初步检测结果显示PEDF可能是一种肺癌发生发展过程中的保护因素。 | 张力建 陈晋峰 柯杨 Robert E Mansel Wen G Jiang | 2006 | 中国肺癌杂志2006，9，2： | 4 |
| 4 | How mammillary fistulas should be managed显示文摘 | Hanavadi S Pereira G Mansel R E | 2005 | Breast J2005，11，2： | 1 |
| 5 | Mutiplex polymerase chain reaction assay for simultaneous detection of Staphylococcus aureus and Streptococcal causes of bovine mastitis 显示文摘 | PHUEKTES P MANSELL P D BROWING G F | 2001 | Journal of Dairy Science2001，84，5： | 1 |
| 6 | Hepatocyte growth factor disrupts tight junctions in human breast cancer cells显示文摘 | Martin TA Watkins G Mansel RE | 2004 | Cell Biol Int2004，28，5： | 1 |
| 7 | The effects of interleukin-7 on the lymphangiogenic properties of human endothelial cells显示文摘 | Al-Rawi M A Watkins G Mansel R E | 2005 | Int J Oncol2005，27，3： | 1 |
| 8 | Hepatocyte growth factor modulates vascular endothelial cadherin expression in human endothelial cells显示文摘 | MARTIN T A MANSEL R JIANG W G | 2001 | Clin Cancer Res2001，7，： | 1 |
| 9 | benetie manipulation of stromal cell-derived-factor-2 attests the pivotal role of theAutoorine SDF-1-CXCR4 pathway in the aggressiveness of breast cancer cells显示文摘 | Kang H Mansel R E Jiang W G | 2005 | Int J Oncol2005，26，5： | 1 |
| 10 | Aberrant ex-pression of 5-lipoxygenase-activating protein(5-LOXAP)hasprognostic and survival significance in patients with breastcancer显示文摘 | Jiang W G Douglas-Jones A G Mansel R E | 2006 | Prostaglandins Leukot Essent Fatty Acids2006，74，2： | 1 |
| 11 | Aberrant expression of 5-lipoxygenase-Activation protein (5- LOXAP) has prognostic and survival significance in patients with breast cancer显示文摘 | Jiang W G Douglas-Jones A G Mansel R E | 2006 | Prostaglandins Leukot Essent Fatty Acid2006，72，2： | 1 |
| 12 | A model for wetland hydrology:description and validation显示文摘 | Mansell R S Bloom S A Sun G | 2000 | Soil Science2000，，165： | 1 |
| 13 | The role of the CD44/ezrin complex in cancer metastasis显示文摘 | MARTIN T A HARRISON G MANSEL R E | 2003 | Crit Rev Oncol Hematol2003，46，2： | 1 |
| 14 | Internal mammary node drainage and its role in sentinel lymph node biopsy: the ini- tial ALMANAC experience显示文摘 | Mansel R E Goyal A Newcombe R G | 2004 | Clin Breast Cancer2004，5，4： | 1 |
| 15 | The role of the CD44/ezrin complex in cancer metastasis显示文摘 | Martin T A Harrison G Mansel R E | 2003 | Crit Rev Oncol Hematol2003，46，： | 1 |
| 16 | The role of the CD44/ Ezrin complex in cancer metastasis 显示文摘 | Martin TA Harrison G Mansel RE | 2003 | Crit Rev Oncol Hematol2003，46，2： | 1 |
| 17 | Simulator transformation and transport of phosphorus in soil显示文摘 | Mansell R S Selim H M Fiskell J G A | 1977 | Soil Sci1977，124，3： | 1 |
| 18 | The role of CD44/ Ezrin complex in cancer metastasis显示文摘 | Matin TA Harrison G Mansel RF | 2003 | Cfit Rev Oncol Hematol2003，6，： | 1 |
| 19 | Simulated three-dimensional branched lightning in a numerical thunderstorm model 显示文摘 | Mansell E R MacGorman D R Ziegler G L | 2002 | Journal of Geophysical Research2002，107，9： | 1 |
| 20 | Correlationships between clearness index and relative sunshine duration for Sudan显示文摘 | ELAGIB N A ALVI S H MANSEL M G | 1999 | Renewable Energy1999，17，4： | 1 |
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