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| 1 | Helicobacter pylori and EBV in gastric carcinomas:Methylation status and microsatellite instability显示文摘AIM:To verify the methylation status of CDH1, DAPK, COX2, hMLH1 and CDKN2A genes and to evaluate their association with Helicobacter pylori (H. pylori)-cagA+ and Epstein Barr virus (EBV) infections in gastric adenocarcinomas.METHODS: Methylation-specific PCR (MSP) assay was performed in 89 primary gastric carcinomas (intestinal and diffuse types). Microsatellite instability (MSI) analysis was performed using the BAT26 primer set and PCR products were analyzed with the ABI PRISM 3100 Genetic Analyzer using Genescan 3.7 software (Applied Biosystems). Detection of H. pylori and genotyping were performed by PCR, using specifi c primers for ureaseC and cagA genes. The presence of EBV was assessed by in situ hybridization. Statistical analyses were performed using the χ2 or Fisher's exact test.RESULTS: The most frequent hypermethylated gene was COX-2 (63.5%) followed by DAPK (55.7%), CDH1 (51%), CDKN2A (36%) and hMLH1 (30.3%). Intestinal and diffuse adenocarcinomas showed different methylation profiles and there was an association between methylation of E-CDH1 and H. pylori-cagA+ in the intestinal adenocarcinoma type. MSI was correlated with hMLH1 methylation. There was an inverse correlation between DAPK hypermethylation and MSI.CONCLUSION: We found a strong association between CDH1 methylation and H. pylori-cagA+ in intestinal-type gastric cancer, association of MSI and better prognosis and an heterogeneous COX-2 overexpression. | Adriana Camargo Ferrasi Nídia Alice Pinheiro Silvia Helena Barem Rabenhorst Otávia Luisa Caballero Maria Aparecida Marchesan Rodrigues Fabrício de Carvalho Celso Vieira de Souza Leite Marcia Valéria Pitombeira Ferreira Marcos Aurélio Pessoa Barros Maria Inês de Moura Campos Pardini | 2010 | World Journal of Gastroenterology2010，16，3： | 6 |
| 2 | MicroRNAs expression influence in ulcerative colitis and Crohn's disease:A pilot study for the identification of diagnostic biomarkers显示文摘BACKGROUND Inflammatory bowel disease(IBD)comprises two distinct diseases,Crohn’s disease(CD)and ulcerative colitis(UC),both of which are chronic,relapsing inflammatory disorders of the gastrointestinal tract with a mostly unknown etiology.The incidence and prevalence of IBD are continually increasing,indicating the need for further studies to investigate the genetic determinants of these diseases.Since microRNAs(miRNAs)regulate protein translation via complementary binding to mRNA,discovering differentially expressed miRNAs(DE)in UC or CD patients could be important for diagnostic biomarker identification,assisting in the appropriate disease differentiation progressing the understanding of IBD pathogenesis.AIM To determine the miRNA expression profile in UC and CD patients and the potential pathophysiological contributions of differentially expressed miRNA.METHODS A total of 20 formalin-fixed paraffin-embedded colonic samples were collected from the Pathology Department of Botucatu Medical School at São Paulo State University(Unesp).The diagnosis of UC or CD was based on clinical,endoscopic,radiologic,and histological criteria and confirmed by histopathological analysis at the time of selection.The TaqMan™Array Human MicroRNA A+B Cards Set v3.0(Applied Biosystems™)platform was used to analyze 754 miRNAs.Targets of DE-miRNAs were predicted using miRNA Data Integration Portal(mirDIP)and the miRNA Target Interaction database(MiRTarBase).All statistical analyses were conducted using GraphPad Prism software.Parametric and nonparametric data were analyzed using t-tests and Mann-Whitney U tests,respectively.RESULTS The results showed that of the 754 miRNAs that were initially evaluated,643 miRNAs were found to be expressed in at least five of the patients who were diagnosed with either CD or UC;the remaining 111 miRNAs were not considered to be expressed in these patients.The expression levels of 28 miRNAs were significantly different between the CD and UC patients(P≤0.05);13 miRNAs demonstrated a fold-change in expression level greater than 1.Five miRNAs with a downregulated expression were selected for enrichment analysis.The miRNAs whose expression levels were significantly lower in UC patients than in CD patients were enriched in certain signaling pathways that were mostly correlated with cancer-related processes and respective biomarkers.CONCLUSION MiRNAs could be used to differentiate UC from CD,and differently expressed miRNAs could help explain the distinct pathophysiology of each disease. | Ana Elisa Valencise Quaglio Felipe Jose Santaella Maria Aparecida Marchesan Rodrigues Ligia Yukie Sassaki Luiz Claudio Di Stasi | 2021 | World Journal of Gastroenterology2021，27，45： | 3 |
| 3 | Challenges in the diagnosis of intestinal neuronal dysplasia type B:A look beyond the number of ganglion cells显示文摘Intestinal neuronal dysplasia type B(IND-B)is a controversial condition among gastrointestinal neuromuscular disorders.Constipation is its most common clinical manifestation in patients.Despite intense scientific research,there are still knowledge gaps regarding the diagnostic criteria for IND-B in the histopathological analysis of rectal biopsies.The guidelines published in the past three decades have directed diagnostic criteria for quantifying the number of ganglion cells in the nervous plexus of the enteric nervous system.However,it is very complex to distinguish numerically what is pathological from what is normal,mainly because of the difficulty in determining a reliable control group composed of healthy children without intestinal symptoms.Thus,a series of immunohistochemical markers have been proposed to assist in the histopathological analysis of the enteric nervous system.Several of these markers facilitate the identification of other structures of the enteric nervous system,in addition to ganglion cells.These structures may be related to the etiopathogenesis of IND-B and represent new possibilities for the histopathological diagnosis of this disease,providing a view beyond the number of ganglion cells.This review critically discusses the aspects related to the disease definitions and diagnostic criteria of this organic cause of constipation. | Simone Antunes Terra Anderson Cesar Gonçalves Pedro Luiz Toledo de Arruda Lourenção Maria Aparecida Marchesan Rodrigues | 2021 | World Journal of Gastroenterology2021，27，44： | 2 |
| 4 | Cabergoline: a safe, easy, cheap, and effective drug for prevention/ treatment of ovarian hyperstimulation syndrome? 显示文摘 | Manno M Tomei F Marchesan E | 2005 | Eur J Obstet Gynecol Reprod Biol2005，122，1： | 1 |
| 5 | Tempera- ture variation at the external root surface during 980nm diode la- ser irradiation in the root canal 显示文摘 | Alfredo E Marchesan MA Sousa - Neto MD | 2008 | J Dent2008，36，7： | 1 |
| 6 | Chemoenzymatic synthesis of GDP-azidodeoxymannoscs: Non-radioactive probes for mannosyltransferase activity 显示文摘 | Marchesan S and Macmillan D | 2008 | Chem Commun2008，36，： | 1 |
| 7 | Taste and odour testing: how valuable is training显示文摘 | MORRAN J MARCHESAN M | 2004 | Water Science and Technology2004，49，9： | 1 |
| 8 | Effects of enamel matrix derivative and transforming growth factor-beta1 on human osteoblastic cells显示文摘 | Palioto DB Rodrigues TL Marchesan JT | 2011 | Head Face Med2011，7，： | 1 |
| 9 | Evaluation of smear layer removal by EDTAC and sodium hypochlorite with ultrasomic agitation显示文摘 | Guerisoli DM Marchesan MA Walmsley AD | 2002 | Int Endod J2002，35，5： | 1 |
| 10 | Taste and odour testing: how valuable is training?显示文摘 | Morran J Marchesan M | 2004 | Water Science and Technology2004，49，9： | 1 |
| 11 | Effect of in vitro gingival fibroblast seeding on the in vivo incorporation of acellular dermal matrix allografts in dogs显示文摘 | Novaes AB Jr Marchesan JT Macedo GO | 2007 | J Periodontol2007，78，2： | 1 |
| 12 | Effects of enamel derivative and transforming growth factor-β1 on human periodontal ligament fibroblasts 显示文摘 | Rodrigues TLS Marchesan JT Coletta RD | 2007 | Clin Periodontal2007，34，： | 1 |
| 13 | Taste and odour testing: how valuable is training? 显示文摘 | Morran J Marchesan M | 2004 | Water Sci Technol2004，49，9： | 1 |
| 14 | Invitro evaluation of endodontic debris removal as obtained by rota-ry instrumentation coupled with ultrasonic irrigation显示文摘 | Passarinho-Neto JG Marchesan MA Ferreira RB | 2006 | AustEndod J2006，32，3： | 1 |
| 15 | Temperature variation at the external root surface during 980-nm diode laser irradiation in the root canal显示文摘 | Alfredo E Marchesan MA Sousa-Neto MD | 2008 | J Dent2008，36，7： | 1 |
| 16 | Behavioral effects of artificial light on fish species of commercial interest显示文摘 | MARCHESAN M SPOTO M VERGINELLA L | 2005 | Fisheries Research2005，73，12： | 1 |
| 17 | Coupled cluster calculations of the ground state potential and interaction induced electric properties of the mixed dimers of helium, neon and argon显示文摘 | J L Caeheim B Femandez D Marchesan | 2004 | Mol Phys2004，102，： | 1 |
| 18 | Scanning laser polarimetry with variable corneal compensation and detection of glaucomatous optic neuropathy 显示文摘 | Da Pozzo S Iacono P Marchesan R | 2005 | Graefe'' s Arch Clin Exp Ophthahnol2005，243，： | 1 |
| 19 | Evaluation of smear layer removal by EDTAC and sodium hypochlorite with ultrasomic agitation显示文摘 | Guerisoli DM Marchesan MA Walmsley AD | 2002 | Int Endod J2002，35，5： | 1 |
| 20 | Scanning laser polarimetry-a review显示文摘 | Da Pozzo S Marchesan R Ravalico G | 2009 | Clin Exp Ophthalmol2009，37，1： | 1 |
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