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| 1 | Transactivation of the TIEG1 confers growth inhibition of transforming growth factor-β-susceptible hepatocellular carcinoma cells显示文摘AIM:To investigate the role of transforming growth factor(TGF)-β-inducible early gene 1(TIEG1) in TGF-β-induced growth inhibition in hepatocellular carcinoma(HCC) cells.METHODS:Human hepatocyte and HCC cell lines with varied susceptibilities to TGF-β1 were tested by methylthiazoletetrazolium(MTT) assay.The expression changes of Smad2,Smad3,Smad4,Smad7,TIEG1 and TIEG2 gene following treatment with TGF-β1 in a TGF-β-sensitive hepatocyte cell line(MIHA),a TGF-β-sensitive hepatoma cell line(Hep3B) and two TGF-β-insensitive hepatoma cell lines(HepG2 and Bel7404) were examined.SiRNA targeting TIEG1 was transfected into Hep3B cells and the sensitivity of cells to TGF-β1 was examined.Overexpression of TIEG1 was induced by lentiviral-mediated transduction in TGF-β1-resistant hepatoma cell lines(Bel7404 and HepG2).MTT assay and 4',6-Diamidino-2-phenylindole staining were used to identify cell viability and apoptosis,respectively.The expression level of stathmin was measured by reverse transcriptase polymerase chain reaction and Western-blotting analysis,and stathmin promoter activity by TIEG1 was monitored by a luciferase reporter gene system.RESULTS:TIEG1 was significantly upregulated by TGF-β1 in the TGF-β1-sensitive HCC cell line,Hep3B,but not in the resistant cell lines.The suppression of TIEG1 by siRNAs decreased the sensitivity of Hep3B cells to TGF-β1,whereas the overexpression of TIEG1 mediated growth inhibition and apoptosis in TGF-β1-resistant HCC cell lines,which resembled those of TGF-β1-sensitive HCC cells treated with TGF-β1.Our data further suggested that stathmin was a direct target of TIEG1,as stathmin was signif icantly downregulated by TIEG1 overexpression,and stathmin promoter activity was inhibited by TIEG1 in a dose-dependent manner.CONCLUSION:Our data suggest that transactivation of TIEG1 conferred growth inhibition of TGF-β-susceptible human HCC cells. | Lei Jiang Yiu-Kay Lai Jin-Fang Zhang Chu-Yan Chan Gang Lu Marie CM Lin Ming-Liang He Ji-Cheng Li Hsiang-Fu Kung | 2012 | World Journal of Gastroenterology2012，18，17： | 13 |
| 2 | Inferring the Tree of Life of the order Cypriniformes, the earth's most diverse clade of freshwater fishes: Implications of varied taxon and character sampling显示文摘The phylogenetic relationships of species are fundamental to any biological investigation, including all evolutionary studies. Accurate inferences of sister group relationships provide the researcher with an historical framework within which the attributes or geographic origin of species (or supraspecific groups) evolved. Taken out of this phylogenetic context, interpretations of evolutionary processes or origins, geographic distributions, or speciation rates and mechanisms, are subject to nothing less than a biological experiment without controls. Cypriniformes is the most diverse clade of freshwater fishes with estimates of diversity of nearly 3,500 species. These fishes display an amazing array of morphological, ecological, behavioral, and geographic diversity and offer a tremendous opportunity to enhance our understanding of the biotic and abiotic factors associated with diversification and adaptation to environments. Given the nearly global distribution of these fishes, they serve as an important model group for a plethora of biological investigations, including indicator species for future cli-matic changes. The occurrence of the zebrafish, Danio rerio, in this order makes this clade a critical component in understanding and predicting the relationship between mutagenesis and phenotypic expressions in vertebrates, including humans. With the tremendous diversity in Cypriniformes, our understanding of their phylogenetic relationships has not proceeded at an acceptable rate, despite a plethora of morphological and more recent mo-lecular studies. Most studies are pre-Hennigian in origin or include relatively small numbers of taxa. Given that analyses of small numbers of taxa for molecular characters can be compromised by peculiarities of long-branch attraction and nodal-density effect, it is critical that significant progress in our understanding of the relationships of these important fishes occurs with increasing sampling of species to mitigate these potential problems. The recent Cypriniformes Tree of Life initiative is an effort to achieve this goal with morphological and molecular (mitochondrial and nuclear) data. In this early synthesis of our understanding of the phylogenetic relationships of these fishes, all types of data have contributed historically to improving our understanding, but not all analyses are complementary in taxon sampling, thus precluding direct understanding of the impact of taxon sampling on achieving accurate phylogenetic inferences. However, recent molecular studies do provide some insight and in some instances taxon sampling can be implicated as a variable that can influence sister group relationships. Other instances may also exist but without inclusion of more taxa for both mitochondrial and nuclear genes, one cannot distinguish between inferences being dictated by taxon sampling or the origins of the molecular data. | Richard L. MAYDEN Kevin L. TANG Robert M. WOOD Wei-Jen CHEN Mary K. AGNEW Kevin W. CONWAY Lei YANG Andrew M. SIMONS Henry L. BART Phillip M. HARRIS Junbing LI Xuzhen WANG Kenji SAITOH Shunping HE Huanzhang LIU Yiyu CHEN Mutstmi NISHIDA Masaki MIYA | 2008 | 植物分类学报2008，46，3： | 5 |
| 3 | Traditional and molecular analyses for fecal indicator bacteria in non-point source subtropical recreational marine waters显示文摘 | Christopher D. Sinigalliano Jay M. Fleisher Maribeth L. Gidley Helena M. Solo-Gabriele Tomoyuki Shibata Lisa R.W. Plano Samir M. Elmir David Wanless Jakub Bartkowiak Rene Boiteau Kelly Withum Amir M. Abdelzaher Guoqing He Cristina Ortega Xiaofang Zhu Mary | 2010 | Water Research2010，，13： | 2 |
| 4 | Expression Profile and Function Analysis of Long Non-coding RNAs in the Infection of Coxsackievirus B3显示文摘The roles of IncRNAs in the infection of enteroviruses have been barely demonstrated.In this study,we used coxsackievirus B3(CVB3),a typical enterovirus,as a model to investigate the expression profiles and functional roles of IncRNAs in enterovirus infection.We profiled IncRNAs and mRNA expression in CVB3-infected HeLa cells by IncRNA-mRNA integrated microarrays.As a result,700 differentially expressed IncRNAs(431 up-regulated and 269 down-regulated)and 665 differentially expressed mRNAs(299 up-regulated and 366 down-regulated)were identified in CVB3 infection.Then we performed IncRNA-mRNA integrated pathway analysis to identify potential functional impacts of the differentially expressed mRNAs,in which IncRNA-mRNA correlation network was built.According to IncRNA-mRNA correlation,we found that XLOC-001188,an IncRNA down-regulated in CVB3 infection,was negatively correlated with NFAT5 mRNA,an anti-CVB3 gene reported previously.This interaction was supported by qPCR detection following siRNA-mediated knockdown of XLOC-001188,which showed an increase of NFAT5 mRNA and a reduction of CVB3 genomic RNA.In addition,we observed that four most significantly altered IncRNAs,SNHG11,RP11-145F16.2,RP11-1023L17.1 and RP11-1021N1.2 share several common correlated genes critical for CVB3 infection,such as BRE and IRF2BP1.In all,our studies reveal the alteration of IncRNA expression in CVB3 infection and its potential influence on CVB3 replication,providing useful information for future studies of enterovirus infection. | Lei Tong Ye Qiu Hui Wang Yunyue Qu Yuanbo Zhao Lexun Lin Yan Wang Weizhen Xu Wenran Zhao Hongyan He Guangze Zhao Mary HZhang Decheng Yang Xingyi Ge Zhaohua Zhong | 2019 | Virologica Sinica2019，34，6： | 2 |
| 5 | Vildagliptin, a dipeptidyl peptidase- IV inhibitor, improves model-assessed beta-cell function in patients with type 2 diabetes 显示文摘 | Mari A Sallas WM He YL | 2005 | J Clin Endocrinol Metab2005，90，8： | 1 |
| 6 | Study of wheat gluten plasticization with fatty acids 显示文摘 | Marion P Andre'as Redl Marie - He'le'ne M | 2003 | Polymer2003，，44： | 1 |
| 7 | Pluronic L-81 ameliorates diabetic symptoms in db/db mice through transcriptional regulation of microsomal triglyceride transfer protein显示文摘AIM: To test whether oral L-81 treatment could im-prove the condition of mice with diabetes and to investigate how L-81 regulates microsomal triglyceride transfer protein (MTP) activity in the liver. METHODS: Genetically diabetic (db/db) mice were fed on chow supplemented with or without L-81 for 4 wk. The body weight, plasma glucose level, plasma lipid profile, and adipocyte volume of the db/db mice were assessed after treatment. Toxicity of L-81 was also evaluated. To understand the molecular mecha-nism, HepG2 cells were treated with L-81 and the effects on apolipoprotein B (apoB) secretion and mRNA level of the MTP gene were assessed.RESULTS: Treatment of db/db mice with L-81 sig-nificantly reduced and nearly normalized their body weight, hyperphagia and polydipsia. L-81 also markedly decreased the fasting plasma glucose level, improved glucose tolerance, and attenuated the elevated levels of plasma cholesterol and triglyceride. At the effective dosage, little toxicity was observed. Treatment of HepG2 cells with L-81 not only inhibited apoB secretion, but also signif icantly decreased the mRNA level of the MTP gene. Similar to the action of insulin, L-81 exerted its effect on the MTP promoter. CONCLUSION: L-81 represents a promising candidate in the development of a selective insulin-mimetic mol-ecule and an anti-diabetic agent. | Wo-Shing Au Li-Wei Lu Sidney Tam Otis King Hung Ko Billy KC Chow Ming-Liang He Samuel S Ng Chung-Man Yeung Ching-Chiu Liu Hsiang-Fu Kung Marie C Lin | 2009 | World Journal of Gastroenterology2009，15，24： | 1 |
| 8 | Tetradecane and hexadecane binary mixtures as phase change materials(PCMs) for cool storage in district cooling systems显示文摘 | He Bo E Mari Gustafsson Fredrik Setterwall | 1999 | Energy1999，24，： | 1 |
| 9 | Vildagliptin,a dipeptidyl peptidase-IV inhibitor,improves model-assessed beta-cel function in patients with type 2 diabetes显示文摘 | Mari A Sal as WM He YL | | 0，，08： | 1 |
| 10 | P18 tumor suppressor gene and progression of oligodendrogliomas to anaplasia 显示文摘 | He J Hoang-Xuan K Marie Y | 2000 | Neurology2000，55，： | 1 |
| 11 | Effects of Income Ine- quality on China's Economic Growth显示文摘 | Qin Duo Marie Anne Cagas Geoffrey Ducanes Xihua He Rui Liu and Shiguo Liu | 2009 | Journal of Policy Modeling2009，31，1： | 1 |
| 12 | Tourist Satisfaction : A Cognitive - Affective Model显示文摘 | Ignacio Rodri' guez del Bosque He' ctor San Mari' n | 2008 | Annals of Tourism Research2008，35，2： | 1 |
| 13 | Tetradecane and hexadecane binary mixtures as phase change materials (PCMS) for cool storage in district cooling systems显示文摘 | Bo He Mari Gustafsson E Fredrik Setterwall | 1999 | Energy1999，24，： | 1 |
| 14 | Vildagliptin, a dipeptidyl peptidase-IV inhibitor, improves model-assessed beta-cell function in patients with type 2 diabetes显示文摘 | Mari A Sallas WM He YL | 2005 | J Clin Endocrinol Metab2005，90，8： | 1 |
| 15 | 显示文摘 | He Bo Mari Gustafsson E Setterwall F | 1999 | Energy1999，24，： | 1 |
| 16 | δ-Catenin promotes E-cadherin processing and activates β-catenin-mediated signaling: Implications on human prostate cancer progression显示文摘 | Hangun Kim Yongfeng He Ilhwan Yang Yan Zeng Yonghee Kim Young-Woo Seo Mary Jo Murnane Chaeyong Jung Jae-Hyuk Lee Jeong-Joon Min Dong-Deuk Kwon Kyung Keun Kim Qun Lu Kwonseop Kim | 2012 | BBA - Molecular Basis of Disease2012，，： | 1 |
| 17 | Vildagliptin, a dipeptidylpeptidase-IVinhibitor, improves model-assessed beta-Cell functionin patients with type 2 diabetes 显示文摘 | MARI A SALLAS W HE Y | 2005 | J Clin Endocrinol Metab2005，90，8： | 1 |
| 18 | Vildagliptin, a dipeptidyl pep- tidase 1V inhibitor, improves model-assessed beta-cell function in patients with type 2 diabetes显示文摘 | Mari A Sallas W He Y etal | 2005 | J Clin Endocrinol Metab2005，90，8： | 1 |
| 19 | Vildagliptin, a dipeptidyl pepti dase-Ⅳ inhibitor, improves model assessed beta-cell function in patients with type 2 diabetes显示文摘 | Mari A Sallas W M He Y L | 2005 | J Clin Endocrinol Metab2005，90，： | 1 |
| 20 | 显示文摘 | He Bo Mari E Gustafsson Fredrik Setterwall | 1999 | Energy1999，24，： | 1 |
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