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| 1 | Clinical observation of salvianolic acid B in treatment of liver fibrosis in chronic hepatitis B显示文摘AIM: To evaluate the clinical efficacy of salvianolic acidB (SA-B) on liver fibrosis in chronic hepatitis B.METHODS: Sixty patients with definite diagnosis of liverfibrosis with hepatitis B were included in the trial.Interferon-γ (IFN-γ) was used as control drug. Thepatients took orally SA-B tablets or received muscularinjection of IFN-γ in the double blind randomized test,The complete course lasted 6 months. The histologicalchanges of liver biopsy specimen before and after thetreatment were the main evidence in evaluation, incombination with the results of contents of serum HA,LN, Ⅳ-C, P-Ⅲ-P, liver ultrasound imaging, andsymptoms and signs.RESULTS: Reverse rate of fibrotic stage was 36.67 % inSA-B group and 30.0 % in IFN-γgroup. Inflammatoryalleviating rate was 40.0 % in SA-B group and 36.67 %in IFN-γ group. The average content of HA and Ⅳ-Cwas significantly lower than that before treatment. Theabnormal rate also decreased remarkably. Overallanalysis of 4 serological fibrotic markers showedsignificant improvement in SA-B group as comparedwith the IFN-γgroup. Score of liver ultrasound imagingwas lower in SA-B group than in IFN-γgroup (HA 36.7 %vs80 %,Ⅳ-C 3.3 % vs23.2 %). Before the treatment,ALT AST activity and total bilirubin content of patientswho had regression of fibrosis after oral administrationof SA-B, were significantly lower than those of patientswho had aggravation of fibrosis after oraladministration of SA-B. IFN-γ showed certain sideeffects (fever and transient decrease of leukocytes,occurrence rates were 50 % and 3.23 %), but SA-Bshowed no side effects.CONCLUSION: SA-B could effectively reverse liverfibrosis in chronic hepatitis B. SA-B was better than IFN-γ in reduction of serum HA content, overall decrease of4 serum fibrotic markers, and decrease of ultrasoundimaging score. Liver fibrosis in chronic hepatitis B withslight liver injury was more suitable to SA-B in anti-fibrotic treatment. SA-B showed no obvious side effects. | Ping Liu Yi-Yang Hu Cheng Liu Hui-Ming Xue Zhi-Qiang Xu Lie-Ming Xu Cheng-Hai Liu Hong-Tu Gu Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China Da-Yuan Zhu Shanghai Institute of Metaria Medica,Chinese Academy of Sciences,Shanghai 200031,China Zhi-Qing Zhang the 4~(th) Hualyin City Hospital,Hualan City,223000,JiangSu Province,China | 2002 | World Journal of Gastroenterology2002，8，4： | 85 |
| 2 | Prevention and treatment of hepatic artery thrombosis after liver transplantation显示文摘BACKGROUND: Hepatic artery thrombosis (HAT) which is a serious complication after orthotopic liver transplanta- tion (OLT) remains a significant cause of graft loss. The purpose of this study was to sum up our experiences in the prevention, diagnosis and management of HAT after liver transplantation. METHODS: From April 1993 to September 2003, a total of 198 patients underwent OLT at our hospital. The hepatic artery was anastomosed using 7/0 prolane with running continuous suture in 96 patients (group 1) and with inter- rupted suture in 102 (group 2). Ultrasonography was per- formed every day in two weeks after operation and selec- tively afterwards. RESULTS: HAT occurred in 6 patients (6.3%, 6/96) of group 1, and in 1 (1%, 1/102) of group 2 (x2=4.027, P= 0.045). Six patients received emergency thrombectomy, and 1 conservative therapy but died from tumor recurrence eventually. Biliary complication developed in 3 patients af- ter thrombectomy of whom 2 died of liver failure and one waited for retransplantation. In the other 3 patients after thrombectomy, 1 died of renal failure, and 2 survived. The mortality of patients with HAT was 57.1% (4/7). CONCLUSIONS: The technique of hepatic arterial anasto- mosis is the key factor for the prevention of HAT. Routine ultrasonography is very important in early detection of HAT after OLT. Biliary complication is a severe outcome secondary to HAT. | Shu-Sen Zheng, Zhi-Yong Yu, Ting-Bo Liang, Wei-Lin Wang, Yan Shen, Min Zhang, Xiao Xu and Jian Wu Hangzhou, China Centre of Organ Transplantation and Department o Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital of Medica College, Zhcjiang University, Hangzhou 310003, China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，1： | 18 |
| 3 | Two New Pentacyclic Triterpenes from Sabia parviflora显示文摘Two new pentacyclic triterpene acids, 1a, 3b-dihydroxyl-olean-12-en-28-oic acid and 1a, 2a, 3b-trihydroxyl-olean-12-en-28-oic acid, were isolated from the arial parts of Sabia parviflora. | Jin CHEN, Bin CHEN, Jun TIAN, Feng E WU* Laboratory of Natural Materia Medica, Chengdu Institute of Biology, Academia Sinica, Chengdu 610041 | 2002 | Chinese Chemical Letters2002，13，4： | 15 |
| 4 | Pharmacokinetics of magnesium lithospermate B after intravenous administration in beagle dogs显示文摘AIM: To develop a specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the pharmacokinetic study of magnesium lithospermate B (MLB), and study the pharmacokinetics of MLB after iv administration in beagle dogs.. METHODS: Each beagle dog was iv administered MLB 3, 6, and 12 mg/kg random. The serum drug concentration was determined by specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) assays. The pharmacokinetic parameters were calculated by Drug and Statistics version 1.0 program.RESULTS: The calibration curve for MLB was linear over a range of 16-4096 μg/L with coefficients of correlation>0.999. The intra- and inter-day precisions (CV) of analysis were <10 %, and accuracy ranged from 90 % to 113%. After iv administration of MLB at the doses of 3, 6, and 12 mg/kg, the CO values for MLB were estimated to be of 24, 47, and 107 mg/L, respectively. The AUC increased with the increasing doses for iv administration, and the mean AUC0-4 values were 109.3, 247.9, and 582.4 mg·min·L^-1, respectively. MLB was distributed and eliminated quickly from central compartment, the mean T1/2a values for MLB at doses of 3, 6, 12 mg/kg were 2.2, 2.7, and 2.9 rain,and the mean T1/2β values were 43, 42, and 42 min, respectively. CONCLUSION: This LC-MS/MS method is rapid, sensitive, and specific for the pharmacokinetic study of MLB. The kinetic process of MLB in beagle dogs in vivo was best fitted to a two-compartment model. For iv administration, the pharmacokinetic parameters of CO and AUC have good linearity among the doses, and MLB was distributed and eliminated quickly in beagle dogs. | Xiao-chuan LI, Chen YU, Wei-kang SUN, Gang-yi LIU, Jing-ying JIA, Yi-ping WANGDepartment of Pharmacology, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203 Central Laboratory, Shanghai Xuhui Central Hospital, Shanghai 200002, China | 2004 | Acta Pharmacologica Sinica2004，25，11： | 13 |
| 5 | Development of a complex scintillation proximity assay for highthroughput screening of PPAR7 modulators显示文摘Aim: To develop a complex high-throughput screening (HTS) assay based on scintillation proximity assay (SPA) technology for identification of novel peroxisome proliferator-activated receptor gamma (PPARγ) modulators. Methods: Fulllength PPARγand retinoid X receptor alpha (RXRα), biotinylated PPAR response element (PPRE), [^3H]BRL49653 and streptavidin-coated FlashPlate or microbead were used to develop an HTS assay based on SPA technology. This ‘ABCDE' method was validated against conventional hydroxyapatite (HA) assay and applied to large-scale screening of 16 000 synthetic compounds and natural product extracts. Results: (1) IC50 values of positive control compounds (BRL49653 and troglitazone) obtained from the ‘ABCDE' method and HA assay were comparable and consistent with those reported elsewhere; (2) Approximately 178 compounds, showing more than 70% competitive inhibition on BRL49653 binding to PPARγ, were identified initially by the ‘ABCDE' method (microbead); (3) Secondary screening using FlashPlate and cross-reactivity studies with RARα, β,γand RXRα,β,γconfirmed that 12 compounds possessed specific PPARγbinding properties including 2 with IC50 values less than 0.5μmol/L and novel chemical structures. Conclusions: The ‘ABCDE' method using either FlashPlate or microbead, is a highly efficient, automatable, and robust tool to screen potential PPARγmodulators in HTS setting. Its application may be expanded to other nuclear receptors that form heterodimers upon activation. | Bin WU Jie GAO Ming-wei WANG~2 The National Center for Drug Screening,Shanghai Institute of Materia Medica,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences Graduate School of Chinese Academy of Sciences,Shanghai 201203,China | 2005 | Acta Pharmacologica Sinica2005，26，3： | 12 |
| 6 | Ginsenoside-Rg_6, a Novel Triterpenoid Saponin from the Stem-Leaves of Panax ginseng C. A. Mey.显示文摘A novel dammarane-type triterpene oligoglycoside, named ginsenoside-Rg6 3, was isolated from the stem-leaves of Panax ginseng C. A. Mey., together with two known ones, 20(S)-ginsenoside-Rg2 1 and 20(R)-ginsenoside-Rg2 2. On the basis of chemical and physicochemical evidence , the structure of ginsenoside-Rg6 have been elucidated as 6-O-(-L-rhamnosyl-(1?2)-(-D-glucopyranosyl-dammarane-(E)-20(22), 24-diene-3(, 6(, 12(-triol. | Xiu Wei YANG, Long Yun LI, Jian Ming TIAN, Zhi Wei ZHANG, Jin Mei YE, Wei Fang Gu (National Research Laboratory of Natural & Biomimetic Drugs, Peking University, Beijing 100083) (Academy of Chinese Traditional Medicine and Materia Medica of Jilin Provinc | 2000 | Chinese Chemical Letters2000，11，10： | 12 |
| 7 | Investigation of the differences between the “COLD” and “HOT” nature of Coptis chinensis Franch and its processed materials based on animal’s temperature tropism显示文摘The description and differentiation of the so-called 'Cold' and 'Hot' natures, the primary 'Drug Naure' of Chinese medicine, is the focus of theoretical research. In this study, the divergency between the 'Cold' and the 'Hot' natures was investigated through examining the temperature tropism of mice affected by Coptis chinensis Franch and its processed materials by using a cold/hot plate differentiating technology. After exposure to C. chinensis Franch, the macroscopic behavioral index of the remaining rate (RR) on a warm pad (40℃ ) significantly increased (P<0.05), suggesting the enhancement of Hot tropism. The internal indexes of adenosine triphosphatase (ATPase) activity and oxygen consuming volume decreased significantly (P<0.05), suggesting the decapability of energy metabolism. This external behavior of Hot tropism might reflect the internal Cold nature of C. chinensis Franch. However, the processed materials of C. chinensis Franch exhibited a different Cold nature in temperature tropism compared with crude C. chinensis Franch (CC): the Cold nature of bile-processed C. chinensis Franch (BC) enhanced while the ginger-processed C. chinensis Franch (GC) changed inversely. The changing sequence was consistent with the theoretical prognostication. It is indicated that the external Cold & Hot natures of Chinese medicine may possibly reflect in an ethological way for the changes of animal’s temperature tropism which might be internally regulated by the body’s energy metabolism. | ZHOU CanPing1,2, WANG JiaBo1, ZHANG XueRu1,2, ZHAO YanLing1, XIA XinHua2, ZHAO HaiPing1, REN YongShen1 & XIAO XiaoHe1 1 China Military Institute of Chinese Meteria Medica, 302 Military Hospital of China, Beijing 100039, China 2 College of Pharmaceutical Sciences, Hunan University of Chinese Medicine, Changsha 410208, China | 2009 | Science China(Life Sciences)2009，52，11： | 11 |
| 8 | Construction and characterization of an experimental ISCOMS-based hepatitis B polypeptide vaccine显示文摘瞄准:描绘生物化学并且基于 HBsAg 的 T 房间 epitopes 从新奇治疗学的多肽准备的一支试验性的 ISCOMS 疫苗的免疫学的性质,和 heptatis B-ISCOMS 被准备并且调查。方法:刺激建筑群(ISCOMS ) 的免疫基于疫苗的包含新奇治疗学的肝炎 B 多肽被分离方法准备,并且它的形成被 SDS-polyacrylamide 胶化电气泳动化学上验证的电子显微镜学和简历设想。在 ISCOMS 以内的肽的数量被 Bradford 试金决定,并且特定的 CTL 反应被 ELISPOT 试金检测。结果:有大约 40nm 的一条直径的 submicroparticle 的典型像笼子的结构被电子显微镜学观察。从 Bradford 试金的结果证明肽加入的水平关于 0.33g .L (-1) 。在到分子的重量标记(3480kDa ) 的第六个乐队的 paralleled 位置结束,一个清楚的乐队在 SDS 页分析被给看,显示进 ISCOMS 的多肽的成功的加入。ISCOMS 交货系统能高效地改进多肽的 immunogenicity 并且由 ELISPOT 试金的结果在 vivo 得到特定的有免疫力的回答,这被建议,它证明生产房间(特定的 CTL 回答) 的 IFN-gamma 被增加(对待 ISCOMS 的组的点:47+/-5, n =3;控制组:5+/-2, n =3 ) 。结论:基于 ISCOMS 的肝炎 B 多肽疫苗成功地与在 vivo 疫苗的短多肽相比被构造,它导致更高的 CTL 回答。 | Xiao-Ju Guan Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 200031,China,previously worked as a postdoc in Institute of Immunology,Third Military Medical University,Chongqing 400038,China Xiao-Jun Guan,Department of Science & Research,Second Military Medical University,Shanghai 200433,China Yu-Zhang Wu Zheng-Cai Jia Tong-Dong Shi Yan Tan,Institute of Immunology,Third Military Medical University,Chongqing 400038,China | 2002 | World Journal of Gastroenterology2002，8，2： | 11 |
| 9 | A New Furostanol Glycoside from Polygonatum odoratum显示文摘A new furostanol component glycosylated only at C-26 was isolated from the rhizomes of Polygonatum odoratum (Mill.) Druce, and its structure was characterized as 22-hydroxy-25(R and S) furost-5-en-12-on-3b, 22, 26-triol 26-O-b-D-glucopyranoside on the basis of spectroscopic techniques and chemical methods. | Hai Lin QIN*, Zhi Hong LI, Peng WANG Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050 | 2003 | Chinese Chemical Letters2003，14，12： | 11 |
| 10 | Expression of the difference between the Cold(Han) and Hot(Re) natures of traditional Chinese medicines(Strobal and Rhubarb) based on the cold/hot plate differentiating assay显示文摘In this study, objective differences between the Cold (Han) and Hot (Re) nature of traditional Chinese medicines, e.g. Strobal and Rhubarb, are determined by using a cold/hot plate differentiation technology. A novel, self-designed cold/hot plate differentiating instrument, with methodological study, was used to investigate the intervention of Strobal and Rhubarb on the temperature tropism of mice. Compared with the ICR and BALB/c mice, it was found that KM mice on the cold/hot plate were more sensitive to the change of temperature, within the tolerant temperature range of 15-40 ℃ . The temperature tropism behavior of mice is influenced by treatment with Rhubarb and Strobal, as is the activity of ATPase in liver tissue. These trends are consistent with the definition of the Cold/Hot nature of Chinese medicines based on traditional Chinese medicinal theory. This study showed that the differences of the Cold/Hot nature of traditional Chinese medicines. might be objectively represented by the temperature tropism of animal by means of cold/hot differentiating assay. | ZHAO HaiPing1,2, ZHAO YanLing1, WANG JiaBo1, LI HanBing1,2, REN YongShen1,2, ZHOU CanPing1,3, YAN Dan1 & XIAO XiaoHe1· 1 China Military Institute of Chinese Materia Medica, 302 Military Hospital, Beijing 100039, China 2 Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China 3 Hunan University of Chinese Medicine, Changsha 410208, China | 2009 | Science China(Life Sciences)2009，52，12： | 10 |
| 11 | SALVIANOLIC ACID G,A CAFFEIC ACID DIMER WITH A NOVEL TETRACYCLIC SKELETON显示文摘Salvianolic acid G,a caffeic acid dimer with a noveltetracyclic skeleton was isolated from the roots of Salviamiltiorrhiza.Its structure was elucidated by chemical and spectralanalysis,especially by 2D NMR analysis. | Chun Bo AI Lian Niang LI Institute of Materia Medica,Chinese Academy of Medical Sciences,Beijing 100050,China | 1991 | Chinese Chemical Letters1991，2，1： | 10 |
| 12 | Alkaloids from Isatis indigotica显示文摘Isaindigotone 1 and 3-(2’-hydroxyphenyl)-4(3H)-quinazolinone 2 were isolated from the roots of Isatis indigotica. Compound 1 was elucidated as a new alkaloid.Compound 2 was discovered for the first time from a natural | Ke Min DENG Xiao Yun WU Gen Jin YANG and Guo Wei QIN (Shanghai Institute of Materia Medica, Chinese Academy of Sciences. Shanghai 200031) (School of Pharmacy, Second Military Medical University, Shanghai 200433) ( Visiting scholar from Dept. of Chemistr | 1997 | Chinese Chemical Letters1997，8，3： | 9 |
| 13 | THE STRUCTURE OF ARMILLARIZIN,A NEW PROTOILLUDANE SESQUITERPENOID AROMATIC ESTER FROM ARMILLARIA MELLEA(VAHL.EX FR)QUEL.显示文摘A new protoilludane sesquiterpenoid aromatic ester,named armillarizin(1)has been isolated from the artificially cultured mycelium of Armillaria me-llea(Vahl.ex Fr)Quel.(Tricholomataceae).Its structure was deduced on the ba-sis of spectral analyses.The relative configuration was established from thenuclear Overhauser effects and from the values of ~1H-~1H coupling constants. | Jun Shan YANG Ya Lun SU Yu Lan WANG Xiao Zhang FENG De Quan YU Xiao Tian LIANG Institute of Materia Medica,Chinese Academy of Medical Sciences,Beijing,100050 | 1990 | Chinese Chemical Letters1990，1，2： | 8 |
| 14 | The Induction of Liver Microsomal Cytochrome P450 by Glycyrrhiza uralensis and Glycyrrhetinic acid in Mice显示文摘The effect of Glycyrrhiza uralensis Fisch (GRZ) aqueous extract and one of its active principles Glycyrrhetinic acid (GRT) on hepatic cytochrome P450 in mice were investigated. Oral administration of GRZ at 10g/kg/d or GRT at 50 mg/kg/d for 7 days was foulld to increase the P450 contents up to 4.6 fold compared with the controls. The activities of aryl hydrocarbon hydroxylase (AHH,3. 1 and 3. 3 fold), aminopyrine N-demethylase (ADM, 4. 2 and 3. 2 folds), and 7-ethoxycumarin O-deethylase (ECOD, 2. 8 and 2. 5 fold) were also shown to be increased. Westem blot analysis showed that the subtypes of P450 isoforms induced selectively by GRZ and GRT included CYP1A1 (1. 8 and 1. 5 fold over that of the control, respectively), CYP2B1 (both 1.3 fold), and CYP2C11(3 .2 and 3 .0 fold). Moreover, significant positive correlation between the P450 content or the isoforms and the corresponding enzyme activities mentioned above was observed. | HU WEN-YU LI YA-WEI HOU YAN-NING HE KAN (Department of Pharmacology, Instiute of Materia Medica, Chinese Academy of Medical Scicnces & Beijing Union Medical College, Beijing 100050, China) CHEN JIE-FU (Yang-Ming Medical University, Taipei) BUT PAUL PU | 1999 | Biomedical and Environmental Sciences1999，12，1： | 8 |
| 15 | Drug screening for influenza neuraminidase inhibitors显示文摘Neuraminidase (NA) is one of the most important targets to screen the drugs of anti-influenza virus A and B. After virtual screening approaches were applied to a compound database which possesses more than 10000 compound structures, 160 compounds were selected for bioactivity assay, then a High Throughput Screening (HTS) model established for influenza virus NA inhibitors was applied to detect these compounds. Finally, three compounds among them displayed higher inhibitory activities, the range of their IC50 was from 0.1 μmol/L to 3μmol/L. Their structural scaffolds are novel and different from those of NA inhibitors approved for influenza treatment, and will be useful for the design and research of new NA inhibitors. The resuit indicated that the combination of virtual screening with HTS was very significant to drug screening and drug discovery. | LIU Ailin, CAO Hongpeng & DU Guanhua Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China | 2005 | Science China(Life Sciences)2005，48，1： | 7 |
| 16 | Induction of Angiogenesis and Neurogenesis by Serum from Rats Treated with Shunaoxin Dropping Pills显示文摘Objective Shunaoxin Dropping Pills(SDPs),a Chinese patent medicine,has been used widely in China for the treatment of headache,amnesia,and insomnia.The aim of the present study is to observe the effect of SDPs on inducing angiogenesis and neurogenesis in vitro.Methods The present testing system using the serum obtained from animals ig treated with SDPs and a co-culture system in vitro was used to investigate if SDPs promotes brain microvascular endothelial cells(BMECs)tube formation and neural differentiation of neural stem/progenitor cells(NSPCs),which plays important roles in angiogenesis and neurogenesis.Results The SDPs serum sampled from rats ig treated with SDPs for 3 d dose-dependently promoted the tube like structure formation of cultured BMECs,and enhanced the fraction of MAP-2 positive cells of NSPCs,which co-cultured with the BMECs and astrocyte.In addition,there was no significant change in the percentage of glial fibrillary acidic protein positive cells.Conclusion Our results show that SDPs serum can induce neural differentiation and BMECs tube formation in vitro. | ZHUANG Peng-wei1,JIANG Yong-bo1,ZHANG Yan-jun1,CUI Guang-zhi1,TONG Yong-ling2,YANG Xiao-hong3,JIANG Zhen1,LIU Li-hua2 1.Tianjin State Key Laboratory of Modern Chinese Medicine,Key Laboratory of Traditional Chinese Medicine Pharmacology,College of Chinese Materia Medica,Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China 2.Tianjin NO.6 Traditional Chinese Medicine Factory,Zhongxin Pharmaceuticals,Tianjin 300401,China 3.Tianjin Zhongxin Pharmaceutical Group Corporation Limited,Tianjin 300193,China | 2011 | Chinese Herbal Medicines2011，3，1： | 7 |
| 17 | SALVIANOLIC ACID H,A NEW DEPSIDE FROM SALVIA CAVALERIEI VAR.SIMPLICIFOLIA显示文摘A new depside named salvianolic acid H was isolated from the aqueous extract of Salviacavaleriei vat.simplicifolia,along with salvianolic acid A,B,C,isosalvianolic acid C,lithospermic acid,rosmarinic acid,R-(+)-β-(3,4-dihydroxyphenyl)lacticacid and protocatechualdehyde.Rosmarinic acid wasobtained as the major component. | Hai Jun ZHANG Lian Niang LI Institute of Materia Medica,Chinese Academy of Medical Sciences 1 Xian Nong Tan Street,Beijing 100050,China | 1993 | Chinese Chemical Letters1993，4，6： | 7 |
| 18 | Design,Synthesis and in vitro Evaluation of a New Class of Novel Cyclooxygenase-2 Inhibitors:3,4-diaryl-3-pyrrolin-2-ones显示文摘design, synthesis and in vivo evaluation of a new class of COX-2 inhibitors 3, 4-diaryl-3-pyrrolin-2-ones are reported. | Ai Ping BAI, Zong Ru GUO*, Wen Hui HU, Fang SHEN, Gui Fang CHENG Institute of Materia Medica, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100050 | 2001 | Chinese Chemical Letters2001，12，9： | 7 |
| 19 | DNA barcoding provides distinction between Radix Astragali and its adulterants显示文摘Based on variable nuclear and/or organellar DNA sequences among vastly divergent species as well as morphologically indistinguishable species, DNA barcoding is widely applicable in species identification, biodiversity studies, forensic analyses, and authentication of medicinal plants. The roots of Astragalus membranaceus and A. membranaceus var. mongholica are commonly used as Radix Astragali in several Asian countries, including China, Japan, and Korea. However, in addition to the two species recorded in the Chinese Pharmacopoeia, there are twenty-three species from different genera including Astragalus, Oxytropis, Hedysarum, and Glycyrrhiza, which have been used as adulterants not only in trading markets but also by the herbal medicine industry. Therefore, a simple, reliable, and accurate classification method is important for distinguishing authentic Radix Astragali from its adulterants. In this study, we acquired data for 37 samples from four related genera within the family Fabaceae. Then we compared four candidate DNA barcoding markers using ITS, matK, rbcL, and coxI sequences from nuclear, chloroplast, and mitochondrial genomes, all commonly used for plants to identify genetic variations among genera, intraspecies, and interspecies. We observed higher divergences among genera and interspecies for ITS, which have the average Kimura 2-parameter distances of 4.5% and 14.1%, respectively, whereas matK was found to have sufficient divergence at the intraspecific level. Moreover, two indels detected in the matK sequence are useful for PCR studies in distinguishing Radix Astragali from its adulterants. This study suggests that the combined barcoding regions of ITS and matK are superior barcodes for Radix Astragali and further studies should focus on evaluating the applicability and accuracy of such combined markers for a wide range of traditional Chinese herbs. | GUO HaiYan1,2, WANG WeiWei2, YANG Ning2, GUO BaoLin3, ZHANG Sun2, YANG RuiJing2, YUAN Ye2, YU JunLin4, HU SongNian2, SUN QiShi1 & YU Jun2 1School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China 2Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, China 3Chinese Academy of Medical Sciences, Peking Union Medical College Institute of Medicinal Plant Development, Beijing 100094, China 4Department of Chemistry, Tonghua Normal College, Tonghua 134002, China | 2010 | Science China(Life Sciences)2010，53，8： | 7 |
| 20 | Two New Furostanol Glycosides from Asparagus cochinchinensis显示文摘Two new furostanol oligoglycosides named as aspacochioside A (1) and B (2) were isolated from the roots of Asparagus cochinchinensis (Lour.) Merr.. Their structures were elucidated to be 3-O-[{a-L-rhamnopyranosyl-(14)}{b-D-glucopyranosyl}]-26-O-[b-D-glucopy- ranosyl]-(25S)-5b-furostane-3b,22a,26-triol 1 and 3-O-[{a-L-rhamnopyranosyl-(14)}{b-D-glu- copyranosyl}]-26-O-[b-D-glucopyranosyl]-22a-methoxy-(25S)-5b-furostane-3b,26-diol 2 on the basis of spectroscopic techniques and chemical methods. | Yong Chun YANG, Sheng Yang HUANG, Jian Gong SHI* Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050 | 2002 | Chinese Chemical Letters2002，13，12： | 7 |
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