|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | CYP24A1 inhibition facilitates the anti-tumor effect of vitamin D3 on colorectal cancer cells显示文摘AIM:The effects of vitamin D3 have been investigated on various tumors, including colorectal cancer (CRC). 25-hydroxyvitamin-D3-24-hydroxylase (CYP24A1), the enzyme that inactivates the active vitamin D3 metabolite 1,25-dihydroxyvitamin D3 (1,25-D3), is considered to be the main enzyme determining the biological halflife of 1,25-D3. During colorectal carcinogenesis, the expression and concentration of CYP24A1 increases significantly, suggesting that this phenomenon could be responsible for the proposed efficacy of 1,25-D3 in the treatment of CRC. The aim of this study was to investigate the anti-tumor effects of vitamin D3 on the human CRC cell line Caco-2 after inhibition of the cytochrome P450 component of CYP24A1 activity. METHODS:We examined the expression of CYP24A1 mRNA and the effects of 1,25-D3 on the cell line Caco-2 after inhibition of CYP24A1. Cell viability and proliferation were determined by means of sulforhodamine-B staining and bromodeoxyuridine incorporation, respectively, while cytotoxicity was estimated via the lactate dehydrogenase content of the cell culture supernatant. CYP24A1 expression was measured by realtime reverse transcription polymerase chain reaction. A number of tetralone compounds were synthesized to investigate their CP24A1 inhibitory activity. RESULTS:In response to 1,25-D3, CYP24A1 mRNA expression was enhanced significantly, in a time- and dose-dependent manner. Caco-2 cell viability and proliferation were not influenced by the administration of 1,25-D3 alone, but were markedly reduced by coadministration of 1,25-D3 and KD-35, a CYP24A1-inhibiting tetralone. Our data suggest that the mechanism of action of co-administered KD-35 and 1,25-D3 does not involve a direct cytotoxic effect, but rather the inhibition of cell proliferation. CONCLUSION:These findings demonstrate that the selective inhibition of CYP24A1 by compounds such as KD-35 may be a new approach for enhancement of the anti-tumor effect of 1,25-D3 on CRC. | János P Kósa Péter Horváth János Wlfling Dóra Kovács Bernadett Balla Péter Mátyus Evelin Horváth Gábor Speer István Takács Zsolt Nagy Henrik Horváth Péter Lakatos | 2013 | World Journal of Gastroenterology2013，19，17： | 5 |
| 2 | Real-time endoarticular ultrasound imaging of the TMJ--a new diagnostic possibility? A cadaver study显示文摘 | Patonay L Nagy K Engelke W | 2002 | Int J Oral Maxillofac Surg2002，31，5： | 1 |
| 3 | Learning words from context显示文摘 | Nagy W E Herman P A and Anderson R C | 1985 | Reading Research Quarterly1985，20，： | 1 |
| 4 | 查看详情显示文摘 | Spevak W Nagy J O Charych D H | | 0，，： | 1 |
| 5 | Learning words from context 显示文摘 | Nagy W E Herman P A & Anderson P C | 1985 | Reading Research Quarterly1985，，20： | 1 |
| 6 | Prepared- ness and cognitive legitimacy as antecedents of new venture funding显示文摘 | Pollack J M Rutherford M W Nagy B G | 2012 | Entrepreneurship Theory and Prac- tice2012，36，5： | 1 |
| 7 | Colonization of porcine small intestine by Escherichia coli:colonization and adhesion factors of pig enteropathogens that lack K88显示文摘 | Isaacson R E Nagy B Moon H W | 1977 | J Infect Dis1977，135，4： | 1 |
| 8 | Direct colorimetric detection of a receptor-ligand interaction by a polymerized bilayer assemble 显示文摘 | Charych D H Nagy J O Spevak W | 1993 | Science1993，261，58： | 1 |
| 9 | Immunization of suckling pigs against enteric enterotoxigenic Escherichia coli infection by vaccinating dams with purified pili显示文摘 | Nagy B Moon H W Isaacson R E | 1978 | Infect Immun1978，21，1： | 1 |
| 10 | Learning words from context显示文摘 | HERMAN P A ANDERSON R C | 1985 | Reading Research Quarterly1985，，20： | 1 |
| 11 | The development of a clinician - administered PTSD scale 显示文摘 | Blake D D Weathers F W Nagy L M | 1995 | Journal of Traumatic Stress1995，8，： | 1 |
| 12 | Simultaneous amplification of the two most frequent mutations of infantile Tay-Sachs disease in single biastomeres 显示文摘 | Sermon K Lissens W Nagy ZP | 1995 | Hum Reprod1995，10，8： | 1 |
| 13 | Genome sequence and analysis of the tuber crop potato显示文摘 | Xu X Pan S Cheng S Zhang B Mu D Ni P Zhang G Yang S Li R Wang J Orjeda G Guzman F Tones M Lnzano R Ponce O Martinez D De la Cruz G Chakrabarti S K Patil V U Skryabin K G Kuznetsov B B Ravin N V Kolganova T V Beletsky A V Mardanov A V Di Genova A Bolser D M Martin D M Li G Yang Y Kuang H Hu Q Xiong X Bishop G J Sagredo B Mejia N Zagorski W Gromadka R Gawor J Szczesny P Huang S Zhang Z Liang C He J Li Y He Y Xu J Zhang Y Xie B Du Y Qu D Bonierbale M Ghislain M Herrera Mdel R Giuliano G Pietrella M Perrotta G Faeella P O 'Brien K Feingold S E Barreiro L E Massa G A Diambra L Whitty B R Vaillancourt B Lin H Massa A N Geoffroy M Lundback S DellaPenna D Buell C R Sharma S K Marshall D F Waugh R Bryan G J Destefanis M Nagy I Milbourne D Thomson S J Fiefs M Jacobs J M Nielsen K L Sonderkaer M Iovene M Tortes G A Jiang J Veilleux R E Bachem C W de Boer J Bonn T Kloosterman B van Eck H Datema E Hekkert B Goverse A van Ham R C Visser R G | 2011 | Nature2011，475，7355： | 1 |
| 14 | The Acquisition of Morphology:Learning the Contribution of Suffixes to the Meanings of Derivatives显示文摘 | Diakidoy IAN Anderson RC | 1993 | Journal of Reading Behavior1993，，25： | 1 |
| 15 | Growth in phonological,orthographic,and morphonological awareness in Grades 1 to 6显示文摘 | Berninger V.W Abbot R.D Nagy W Gartisle J | | 0，，02： | 1 |
| 16 | Unraveling the Web Services Web: An Introduction to SOAP, WSDL, and UDDI显示文摘 | F Curbera M Duftler R Khalaf W Nagy | 2002 | IEEE Internet Computing2002，6，2： | 1 |
| 17 | Dopamine-derived sal- solinol derivatives as endogenous monoamine oxidase inhibitors: occurrence, metabolism and function in human brains 显示文摘 | NAOI M MARUYAMA W NAGY G M | 2004 | Neuro- toxicology2004，25，12： | 1 |
| 18 | Learning Words from Context显示文摘 | NAGY W HERMAN P ANDERSON R | 1985 | Reading Research Quarterly1985，，20： | 1 |
| 19 | The Acquisition of English Derivational Morphology显示文摘 | Nagy W | 1989 | Journal of Memory and Language1989，，28： | 1 |
| 20 | Learning word meanings from context during normal leading显示文摘 | Nagy W · E R · C Anderson P · A.Herman | | 0，，24： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员