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| 1 | Tenascin C upregulates interleukin-6 expression in human cardiac myofibroblasts via toll-like receptor 4显示文摘AIM:To investigate the effect of Tenascin C(TNC)on the expression of pro-inflammatory cytokines and matrixmetalloproteinases in human cardiac myofibroblasts(CMF).METHODS:CMF were isolated and cultured from patients undergoing coronary artery bypass grafting.Cultured cells were treated with either TNC(0.1μmol/L,24 h)or a recombinant protein corresponding to different domains of the TNC protein;fibrinogen-like globe(FBG)and fibronectin typeⅢ-like repeats(TNⅢ5-7)(both 1μmol/L,24 h).The expression of the proinflammatory cytokines;interleukin(IL)-6,IL-1β,TNFαand the matrix metalloproteinases;MMPs(MMP1,2,3,9,10,MT1-MMP)was assessed using real time RT-PCR and western blot analysis.RESULTS:TNC increased both IL-6 and MMP3(P<0.01)mR NA levels in cultured human CMF but had no significant effect on the other markers studied.The increase in IL-6 mR NA expression was mirrored by an increase in protein secretion as assessed by enzymelinked immunosorbant assay(P<0.01).Treating CMF with the recombinant protein FBG increased IL-6mR NA and protein(P<0.01)whereas the recombinant protein TNⅢ5-7 had no effect.Neither FBG nor TNⅢ5-7 had any significant effect on MMP3 expression.The expression of toll-like receptor 4(TLR4)in human CMF was confirmed by real time RT-PCR,western blot and immunohistochemistry.Pre-incubation of cells with TLR4neutralising antisera attenuated the effect of both TNC and FBG on IL-6 mR NA and protein expression.CONCLUSION:TNC up-regulates IL-6 expression in human CMF,an effect mediated through the FBG domain of TNC and via the TLR4 receptor. | Azhar Maqbool Emma J Spary Iain W Manfield Michaela Ruhmann Lorena Zuliani-Alvarez Filomena O Gamboa-Esteves Karen E Porter Mark J Drinkhill Kim S Midwood Neil A Turner | 2016 | World Journal of Cardiology2016，8，5： | 4 |
| 2 | Cytokine therapy:a natural alternative for disease control显示文摘 | Lowenthal J W O Neil T E | 1999 | Vet Immunol Immunopathol1999，，72： | 1 |
| 3 | Extension of human cell lifespan by nicotinamide phosphoribosyltransferase显示文摘 | van der Veer E Ho C O'Neil C | 2007 | J Biol Chem2007，282，10： | 1 |
| 4 | Pre-B-cell colony-enhancing factor regulates NAD+-dependent protein deacetylase activity and promotes vascular smooth muscle cell maturation显示文摘 | van der Veer E Nong Z O′Neil C | 2005 | Circ Res2005，97，1： | 1 |
| 5 | Salinity effects on the microwave emission of soil显示文摘 | Jackson T J O'Neil P E | 1990 | Remote Sens Environ1990，31，： | 1 |
| 6 | Recombinant chicken IFN-γ expressed in Escherichia coli: Analysis of C-terminal truneation and effeet on biologie aetivity显示文摘 | Miehalski W P Shiell B J O'Neil T E | 1999 | Journal of Interferon & Cytokine Researeh1999，19，4： | 1 |
| 7 | Quantitative ex-pression of osteopontin in nasal mucosa of patients with allergic rhi-nitis :effects of pollen exposure and nasal glucocorticoid treatment显示文摘 | O’NEIL S E MALMHALL C SAMITAS K | 2010 | Allergy Asthma and Clinical Immunology2010，6，1： | 1 |
| 8 | Pre-B-cell colony-enhancing factor regulates NAD +-dependent protein deacetylase activity and promotes vascular smooth muscle cell maturation 显示文摘 | van der Veer E Nong Z O'Neil C | 2005 | Circ Res2005，97，1： | 1 |
| 9 | Pre-B-cell colonyenhancing factor regulates NAD +-dependent protein deacetylase activity and promotes vascular smooth muscle cell maturation显示文摘 | van der Veer E Nong Z O' Neil C | 2005 | Circ Res2005，97，1： | 1 |
| 10 | Potential use of cytokine therapy in poultry显示文摘 | Lowenthal J W York J J O'Neil T E | 1998 | Vet Immunol Immunopathol1998，63，12： | 1 |
| 11 | An optimality proof of the LRU-K page replacement algorithm显示文摘 | O'NEIL E 1 O'NEIL P E WEIKUM G | 1999 | Journal of the ACM1999，46，1： | 1 |
| 12 | lYre-B-cell colony-enhan- cing factor regulates NAD + -dependent protein deacetylase activity and promotes vascular smooth muscle cell maturation显示文摘 | Van der Veer E Nong Z O'Neil C | 2005 | Circ Res2005，97，1： | 1 |
| 13 | Soybean aphid pred- ators and their use in integrated pest management 显示文摘 | Rutledge C E O'Neil R J Fox T B | 2004 | Ann En- tomol Soc Am2004，97，： | 1 |
| 14 | Multi-table joins through bitmapped join indices显示文摘 | O'Neil P E Graefe G | 1995 | SIGMOD Record1995，24，3： | 1 |
| 15 | A rapid and precise method of oxygen isotope analysis of biogenic phosphate显示文摘 | O'Neil J R Roe L J Reinhard E | 1994 | Israel Journal of Earth Science1994，43，34： | 1 |
| 16 | Pre-B-cell colony-enhancing factor regulates NAD + dependent protein deacetylase activity and promotes vascular smooth muscle cell maturation 显示文摘 | vander Veer E Nong Z O' Neil C | 2005 | Circ Res2005，97，1： | 1 |
| 17 | The escrow transactional method显示文摘 | O'NEIL P E | 1986 | ACM Trans Database Syst1986，，11： | 1 |
| 18 | High-Level Production of Recombinant Chicken Interferon-γ by Brevibacillus choshinensis 显示文摘 | Yashiro K Lowenthal J W O'Neil T E | 2001 | Protein Expression and Purification2001，23，1： | 1 |
| 19 | Long-term safety of rituximab in rheumatoid arthritis: 9.5-year follow-up of the global clinical trial programme with a focus on adverse events of interest in RA patients显示文摘 | Ronald F van Vollenhoven Paul Emery Clifton O Bingham Edward C Keystone Roy M Fleischmann Daniel E Furst Nicola Tyson Neil Collinson Patricia B Lehane | 2013 | Annals of the Rheumatic Diseases2013，，9： | 1 |
| 20 | 显示文摘 | van der Veer E Nong Z O'Neil C | 2005 | Circ Res2005，97，1： | 1 |
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