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	题名	作者	年代	出处	被引量
1	Expression of Acyl-lipid 12-desaturase Gene in Prokaryotic and Eukaryotic Cells and Its Effect on Cold Stress Tolerance of Potato显示文摘We report the expression profile of acyl-lipid 12-desaturase (desA) gene from Synechocystis sp. PCC6803 and its effect on cell membrane lipid composition and cold tolerance in prokaryotic (Escherichia coli) and eukaryotic (Solanum tuberosum) cells. For this purpose, a hybrid of desA and reporter gene encoding thermostable lichenase (licBM3) was constructed and used to transform these cells. The expression of this hybrid gene was measured using qualitative (Petri dish test, electrophoregram and zymogram) and quantitative methods (spectrometry and gas liquid chromatography assays). The maximum level of linoleic acid in the bacterial cells containing hybrid gene was 1.9% of total fatty acids. Cold stress tolerance assays using plant damage index and growth parameters showed that cold tolerance was enhanced in primary transgenic lines because of increased unsaturated fatty acid concentration in their lipids. The greatest content of 18:2 and 18:3 fatty acids in primary transgenic plants was observed for lines 2 (73%) and 3 (41%). Finally, our results showed that desaturase could enhance tolerance to cold stress in potato, and desaturase and lichenase retain their functionality in the structure of the hybrid protein where the enzymatic activity of target gene product was higher than in the case of reporter lichenase gene absence in the construction.	Reza Maali Amiri Natalia O. Yur'eva Khristina R. Shimshilashvili Irina V. Goldenkova-Pavlova Vasiliy P. Pchelkin Elmira I. Kuznitsova Vladimir D. Tsydendambaev Tamara I. Trunova Dmitry A. Los Gholamreza Salehi Jouzani Alexander M. Nosov	2010	Journal of Integrative Plant Biology2010，52，3：	6
2	Antitumor activity and safety of tivozanib ( AV-951 ) in a phase II randomized discontinuation trial in patients with renal cell carcinoma 显示文摘	Nosov DA Esteves B Lipatov ON	2012	J Clin Oncol2012，30，14：	1
3	Tivozanib versus sorafenib as initial targeted therapy for patients with metastatic renal cell carcinoma : resultsfrom a phase Ill trial显示文摘	Motzer RJ Nosov D Eisen T	2013	J Clin Oneol2013，31，30：	1
4	Functions of plant secondary metabolites in-vivo and in-vitro显示文摘	Nosov A M	1994	Journal of Plant Physiology1994，41，6：	1
5	Cold shock domain proteins in extremophyte Thellungiella salsuginea(salt cress): gene structure and differential response to cold显示文摘	TARANOV V V BERDNIKOVA M V NOSOV A V	2010	Mol Biol2010，44，5：	1
6	1H and 31P Mas NMR studies of solid heteropolyacids and H3PW12O40 supported on SiO2显示文摘	Mastikhin V M Kulikov S M Nosov A V	1990	J Mol Catal1990，60，：	1
7	1990 1H and 31 P MAS NMR Studies of Solid Het-eropolyacids and H3PW12 O40 Supported on SiO2显示文摘	Mastikhin V M Kulikov S M Nosov A V	1990	Journal of Molecular Catalysis1990，60，1：	1
8	Investigation of heat transfer in pulsed flow of air in pipes 显示文摘	Mamayev V V Nosov V S Syromyatnikov N I	1976	Heat Transfer-Soviet Research1976，8，3：	1
9	AC loss of a model 5 m 2G HTS power cable using wires with NiW sub- strates 显示文摘	Vysotsky V Shutov K Nosov A	2010	Journal of Physics : Conference Series2010，234，03：	1
10	Role of lentivirus-mediated overexpression of programmed death-ligand 1 on corneal allograft survival显示文摘	Nosov M Wilk M Morcos M	2012	Am J Transplant2012，12，5：	1
11	^1H and 31^p Mas NMR studies of solid heteropoly acids and H3PW12 O40 supported on SiO2 显示文摘	Mastikhin V M Kulikov S M Nosov A V	1990	Journal of molecular catalysis A: Chemical1990，60，：	1
12	On the theory of spatially limited light beam displacements in a randomly inhomogeneous medium 显示文摘	Mironov V L Nosov V V	1977	J Opt Soc Am1977，67，8：	1
13	Validation of serum biomarkers for detection of early-stage ovarian cancer显示文摘	Nosov V Su F Amneus M	2009	Am J Obstet Gynecol2009，200，：	1
14	Silica-supported zirconocene catalysis:Preparation,characterization and activity in ethylene polymerization显示文摘	MOROZ B L SEMIKOLENOVA N V NOSOV A V	1998	J Molecular Catalysis A1998，130，：	1
15	Applications of hydrogen plasticizing of titanium alloys显示文摘	NOSOV V K OVCHINNIKOV A V SHCHUGOREV Y Y	2008	Metal Science and Heat Treatment2008，50，78：	1
16	Updatedactivity and safety results of a phase II randomizeddiscontinuation trial(RDT)of AV-951,a potent andselective VEGFR1,2,and 3 kinase inhibitor,in patientswith renal cell carcinoma(RCC)显示文摘	Bhargava P Esteves B Nosov D A	2009	J Clin Oncol2009，27，15：	1
17	Validation of serum biomarkers for detection of early-stage ovarian cancer显示文摘	Nosov V Su F Amneus M	2009	Am J Obstet Gynecol2009，200，6：	1
18	Retarded systems of neutral type显示文摘	Nosov V R	1984	Automat Renote co3ntrol1984，45，1：	1
19	Handbook of fluidization and fluid-particle systems显示文摘	Gelperin N I Einstein V G Nosov G A	1967	Theor Osnovy Khim Technol1967，，1：	1
20	Tivozanib versus sorafenib as initial targeted therapy for patients with metastatic renal cell carcinoma:results from a phase Ⅲ trial显示文摘	Motzer RJ Nosov D Eisen T	2013	J Clin Oncol2013，31，：	1

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