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| 1 | Combination of Cyclamen persicum Mill. floral gene promoters and chimeric repressors for the modification of ornamental traits in Torenia fournieri Lind.显示文摘Although chimeric repressors such as the Arabidopsis TCP3 repressor are known to have significant effects on flower morphology and color,their cellular-level effects on flower petals are not understood.The promoter sequences of the genes expressed in the flowers of cyclamen,a representative potted flower grown during the winter season,are also unknown.Here,we isolated eight promoters from cyclamen genes that are reportedly expressed in the petals.These promoters were then fused to four chimeric repressors and introduced into the model flower torenia to screen for effective combinations of promoters and repressors for flower breeding.As expected,some of the constructs altered flower phenotypes upon transformation.We further analyzed the effects of chimeric repressors at the cellular level.We observed that complicated petal and leaf serrations were accompanied by excessive vascular branching.Dichromatism in purple anthocyanin was inferred to result in bluish flowers,and imbalanced cell proliferation appeared to result in epinastic flowers.Thus,the genetic constructs and phenotypic changes described in this report will benefit the future breeding and characterization of ornamental flowers. | Ichiro Kasajima Norihiro Ohtsubo Katsutomo Sasaki | 2017 | Horticulture Research2017，4，1： | 2 |
| 2 | Cell cycle progression and apoptosis after irradiation in an acidic environment cell death 显示文摘 | Park H J Lyons JC Ohtsubo T | 2000 | Differ2000，7，： | 1 |
| 3 | PCPA,which is involved in the degradation of pentachlorophenol in Sphingomonas chlorophenolica ATCC39723,is a novel type of ring-cleavage dioxygenase显示文摘 | OHTSUBO Y MIYAUCHI K KANDA K | 1999 | Federation of European Biochemical Societies Letters1999，459，3： | 1 |
| 4 | Effects of sequence and temperature of hyperthermia and peplomyein on human pharyngeal carcinoma KB cells in Vitro显示文摘 | Ohtsubo T Saito H Hoda I | 1996 | Neoplasma1996，43，3： | 1 |
| 5 | Development of rice quality evaluating technique based on physical properties of cooked rice 显示文摘 | SHIMZU N KIMURA T OHTSUBO K | 1997 | Journal of Japanese Society Agricultural Machinery1997，59，： | 1 |
| 6 | Ariake Bay quick clays: A comparison with general model显示文摘 | Ohtsubo M | 1995 | Soils and Foundations1995，35，1： | 1 |
| 7 | Effect of acidic environ- ment and p53 on apoptosis induction by hyperthermia 显示文摘 | Ohtsubo T Park HJ Lyons JC | 2000 | Int J Hyperthermia2000，16，6： | 1 |
| 8 | Examination of catalytic roles of inherent metallic species in steam reforming of nascent volatiles from the rapid pyrolysis of a brown coal显示文摘 | Masek O Sonoyama N Ohtsubo E | 2007 | Fuel Process Teehnol2007，88，2： | 1 |
| 9 | An ultra performance liquid chromatographic method for determining phytosterol uptake by Caco- 2 cells显示文摘 | ITO N OHTSUBO T KUSU F | 2012 | Analytical Biochemistry2012，421，1： | 1 |
| 10 | Repetitive sequences: cause for variation in genome size and chromosome morphology in the genus Oryza显示文摘 | Sakurako Uozu Hiroshi Ikehashi Nobuko Ohmido Hisako Ohtsubo Eiichi Ohtsubo Kiichi Fukui | 1997 | Plant Molecular Biology1997，，6： | 1 |
| 11 | Granulocyte - colony - stimulating factor enhances invasive potential of human head - and- neck- carcinoma cell lines 显示文摘 | Noda 1 Fujieda S Ohtsubo T | 1999 | Int J Cancer1999，80，1： | 1 |
| 12 | Corefucose and bisecting GlcNAc,the direct modifiers of theN-glycan core: their functions and target proteins 显示文摘 | Takahashi M Kuroki Y Ohtsubo K | 2009 | Carbohydr Res2009，344，12： | 1 |
| 13 | Dependence of biodegradation and release behavior on physical proper- ties of poly ( caprolactone ) - based polyurethanes显示文摘 | Watanabe A Takebayashi Y Ohtsubo T | 2009 | Journal of Applied Polymer Science2009，114，1： | 1 |
| 14 | Endosonographic evaluation of c-kit-positive gastrointestinal stromal tumor显示文摘 | Okai T Minamoto T ohtsubo K | 2003 | Abdow Lmaging2003，28，3： | 1 |
| 15 | Human cyclin E,a nuclear protein essential for the G1-to-S phase transition显示文摘 | Ohtsubo M Theodoras AM Schumacher J | | 0，，05： | 1 |
| 16 | Troglitazone induces p27kipl -associated cell-cycle arrest through down-regulating skp2in human hepatoma cells显示文摘 | Koga H Harada M Ohtsubo M | 2003 | Hepatology2003，37，5： | 1 |
| 17 | Binding of 14-3-3β but not 14-3-3σ controls the cytoplasmic localization of CDC25B:binding site preferences of 14-3-3 subtypes and the subcellular localization of CDC25B显示文摘 | Uchida S Kuma A Ohtsubo M | 2004 | Journal of Cell Science2004，117，14： | 1 |
| 18 | Natural progression of untreated hepatolithiasis that shows no clinical signs at its initial presentation 显示文摘 | KUSANO T ISA T OHTSUBO M | 2001 | J Clin Gastroenterol2001，33，2： | 1 |
| 19 | Glycosylation in cellular mechanisms of health and disease显示文摘 | Ohtsubo K Marth JD | 2006 | Cell2006，126，5： | 1 |
| 20 | Purification and properties of a heat stable inulin fructotransferase(DFAlll-producing) from Arthrobacter pascens T13 - 2 显示文摘 | Kazutomo Haraguchi Tomomi Yamanka Kenichi Ohtsubo | 2002 | Carbohydrate Polymers2002，50，： | 1 |
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