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| 1 | Highly active antiretroviral therapy dysregulates proliferation and differentiation of human pre-adipocytes显示文摘AIM To investigate the mechanism(s) by which potential effects of multi-drug highly-active antiretroviral therapy contributes to lipodystrophy syndrome. METHODS Preadipocytes from healthy donors were assessed for proliferation and differentiation in the presence of nucleoside reverse transcriptase inhibitors(NRTIs), nonnucleoside reverse transcriptase inhibitors(NNRTIs), and protease inhibitors(PIs) individually and in combination. Effects on proliferation were assessed with a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay and effects on differentiation were assessed from glycerol-3-phosphate dehydrogenase(GPDH) activity and quantitation of Oil Red O staining for intracellular lipid. Data were analyzed with a randomized block ANOVA with post-hoc Fisher's Least Significant Difference test. RESULTS Preadipocyte proliferation was inhibited by a combination of NNRTI + NRTI(14% at 48 h, P < 0.001) and PI + NRTI(19% at 48 h, P < 0.001) with additional suppression when ritonavir(RTV) was added(26% at 48 h). The drug combination of atazanavir(ATV) + RTV + emtricitabine(FTC) + tenofovir(TDF) had the greatest inhibitory effect on proliferation at 48 h. Preadipocyte differentiation was most significantly reduced by the efavirenz + FTC + TDF assessed either by GPDH activity(64%) or lipid accumulation(39%), P < 0.001. Combining NRTIs with a PI(ATV + FTC + TDF) significantly suppressed differentiation(GPDH activity reduced 29%, lipid accumulation reduced by 19%, P < 0.01). This effect was slightly greater when a boosting amount of RTV was added(ATV + FTC + TDF + RTV, P < 0.001). CONCLUSION Although combination antiretroviral therapy is clinically more efficacious than single drug regimens, it also has a much greater inhibitory effect on preadipocyte proliferation and differentiation. | Eyone Jones Pavel Mazirka Margaret A McNurlan Frank Darras Marie C Gelato Giuseppe Caso | 2017 | World Journal of Virology2017，6，3： | 3 |
| 2 | Toll-like receptor 4 polymorphisms and bacterial infections in patients with cirrhosis and ascites显示文摘AIM To assess the relationship between the presence of toll-like receptor 4(TLR4) polymorphisms and bacterial infections in cirrhotic patients with ascites. METHODS We prospectively included consecutive patients with cirrhosis and ascites hospitalized during a 6-year period. Patients with human immunodeficiency virus(HIV) infection or any other immunodeficiency, patients with advanced hepatocellular carcinoma(beyond Milan's criteria) or any other condition determining poor short-term prognosis, and patients with a permanent urinary catheter were excluded. The presence of D299 G and/or T399 I TLR4 polymorphisms was determined by sequencing and related to the incidence and probability of bacterial infections, other complications of cirrhosis, hepatocellular carcinoma, and mortality during follow-up. A multivariate analysis to identify predictive variables of mortality in the whole series was performed. RESULTS We included 258 patients: 28(10.8%) were carriers of D299G and/or T399I TLR4 polymorphisms(polymorphism group) and 230 patients were not(wildtype group). The probability of developing any bacterial infection at one-year follow-up was 78% in the polymorphism group and 69% in the wild-type group(P = 0.54). The one-year probability of presenting infections caused by gram-negative bacilli(51% vs 44%, P = 0.68), infections caused by gram-positive cocci(49% vs 40%, P = 0.53), and spontaneous bacterial peritonitis(29% vs 34%, respectively, P = 0.99) did not differ between the two groups. The oneyear probability of transplant-free survival was 55% in the polymorphism group and 66% in the wild-type group(P = 0.15). Multivariate analysis confirmed that age, Child-Pugh score, active alcohol intake, previous hepatic encephalopathy, hepatocellular carcinoma and serum creatinine were associated with a higher risk of death during follow-up. CONCLUSION Genetic polymorphisms D299 G and/or T399 I of TLR4 do not seem to play a relevant role in the predisposition of cirrhotic patients with ascites to bacterial infections. | Edilmar Alvarado-Tapias Carlos Guarner-Argente Elida Oblitas Elisabet Sánchez Silvia Vidal Eva Román Mar Concepción Maria Poca Cristina Gely Oana Pavel Juan Camilo Nieto Cándido Juárez Carlos Guarner Germán Soriano | 2018 | World Journal of Hepatology2018，10，1： | 3 |
| 3 | Heterogeneity of c-myc expression in histologically similar infiltrating ductal carcinomas of the breast 显示文摘 | PAVELIC ZP PAVELIC K CARTER C P | 1992 | J Cancer Res Clin Oncol1992，118，1： | 1 |
| 4 | The role of insulin-like growth factor 2 and its receptors in human tumors显示文摘 | PAVELIC K BUKOVI(C)D PAVELI(C) J | 2002 | Mol Med2002，8，12： | 1 |
| 5 | Selectivegrowth of SWNTs on partially reduced monometalliccobait catalyst 显示文摘 | Maoshuai H Alexander I C Pavel V F | 2011 | Chem Commun2011，47，： | 1 |
| 6 | Characteristics of thermal cycling in a magnesium alloy composite显示文摘 | Frantisek C Pavel L Jens K | 2002 | Materials Science and Engineering2002，325，： | 1 |
| 7 | Molecular identification of bacteria from a cocultore by Denaturing Gradient Gel Electro- phoresis of 16S Ribosomal DNA fragments as a tool for isolation in pure cultures 显示文摘 | Andreas T Pavel S Yehuda C | 1996 | Applied and Environmental Microbiolo- gy1996，62，： | 1 |
| 8 | The interaction between classification and rejectperformance for distance-based reject-option classifiers显示文摘 | Thomas C Landgrebe David M Tax Pavel Paclik | 2006 | Pattern Recognition Letters2006，27，8： | 1 |
| 9 | Gastric cancer:the role of insulin-like growth factor 2 (IGF2) and its receptors (IGF-1R and M6P/IGF-2R)显示文摘 | PAVELIC K KOLAK T KAPITANOVI(C)S | 2003 | J Pathol2003，201，3： | 1 |
| 10 | The role of insulin-like growth factor 2 and its receptors in human tumors显示文摘 | PAVELIC K BUKOVI(C) D PAVELI(C) J | 2002 | Mol Med2002，8，12： | 1 |
| 11 | The X3LYP extended density functional accurately describes H-bonding but fails completely for stacking显示文摘 | Jiǐi (C)erey Pavel Hobza | 2005 | Phys Chem Chem Phys2005，7，： | 1 |
| 12 | Separation of twenty underivatized essen- tial amino acids by capillary zone electrophoresis with contactless conductivity detection 显示文摘 | Pavel C Jiu Z Tom G | 2003 | Electrophoresis2003，24，4： | 1 |
| 13 | Computationally de- signed monomers for molecular imprinting of chemical warfare agents 显示文摘 | Pavel D Lagowski J Lepage C J | 2006 | Polymer2006，47，： | 1 |
| 14 | 显示文摘 | Pavel C Zverev P G Jelinkova H | 2000 | Optics Communications2000，177，： | 1 |
| 15 | How can SEMs effectively implement the CSR agenda? a UK case study perspective 显示文摘 | Pavel C Michaela A B Christopher J B | 2004 | Corporate Social Responsibility and Environmental Management2004，11，3： | 1 |
| 16 | High performance liquid chromatography-mass spectrometric analysis of sulphonated dyes and intermediates显示文摘 | Michal Hol(c)apek Pavel Jandera Petr Zderadi(c)ka | | 0，，： | 1 |
| 17 | Staphylococcal scalded skin syndrome in the Czech Republic: an epidemiological study显示文摘 | Betislav L Pavel B Zuzana C | 2012 | Bums2012，38，2： | 1 |
| 18 | A new approach for modeling slag infiltration and solidification in a continuous casting mould显示文摘 | PAVEL E R L PETER D L KENNETH C M | 2010 | ISIJ International2010，50，12： | 1 |
| 19 | Development of hyperdynamic circulation and response to β‐blockers in compensated cirrhosis with portal hypertension显示文摘 | Càndid Villanueva Agustín Albillos Joan Genescà Juan G. Abraldes Jose L. Calleja Carles Aracil Rafael Ba?ares Rosa Morillas María Poca Beatriz Pe?as Salvador Augustin Joan Carles Garcia‐Pagan Oana Pavel Jaume Bosch | 2016 | Hepatology2016，，1： | 1 |
| 20 | ’All-inclusive’ imaging of the rutileTiO<SUB>2</SUB>(110) surface using NC-AFM显示文摘 | Ralf Bechstein César González Jens Schütte Pavel Jelínek Rubén Pérez Angelika Kühnle | 2009 | Nanotechnology2009，，50： | 1 |
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