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| 1 | Expression of p53,Bax and Bcl-2 proteins in hepatocytes in non-alcoholic fatty liver disease显示文摘AIM: To analyze the protein expression essential for apoptosis in liver steatosis. METHODS: The expression of proapoptotic proteins p53, Bax, and antiapoptotic Bcl-2 in hepatocytes with steatosis (SH) and without steatosis (NSH) was evaluated in 84 patients at various stages of non-alcoholic fatty liver disease (NAFLD). RESULTS: Immunohistochemical staining of liver tissue showed the activation of p53 protein in SH and NSH with increased liver steatosis, diminished Bcl-2 and slightly decreased Bax protein. Positive correlation was found between the stage of liver steatosis with p53 expression in SH (r = 0.54, P < 0.01) and NSH (r = 0.49, P < 0.01). The antiapoptotic protein Bcl-2 was diminished together with the advancement of liver steatosis, especially in non-steatosed hepatocytes (r =0.43, P < 001). CONCLUSION: Apoptosis is one of the most important mechanisms leading to hepatocyte elimination in NAFLD. The intensification of inflammation in NAFLD induces proapoptotic protein p53 with the inhibition of antiapoptotic Bcl-2. | Anatol Panasiuk Janusz Dzieciol Bozena Panasiuk Danuta Prokopowicz | 2006 | World Journal of Gastroenterology2006，12，38： | 46 |
| 2 | Blood platelet and monocyte activations and relation to stages of liver cirrhosis显示文摘AIM: Blood platelets (plt) and monocytes are the cells that play a crucial role in the pathogenesis of liver damage and liver cirrhosis (LC). In this paper, the analysis of mutual relationship between platelets and monocytes activation in LC was conducted.METHODS: Immunofluorescent flow cytometry was usedto measure the percentage of activated platelet populations(CD62P, CD63), the percentage of plt-monocyte aggregates (pma) (CD41/CD45), and activated monocytes (CD11b, CD14, CD16) in the blood of 20 volunteers and 40 patientswith LC. Platelet activation markers: sP-selectin, platelet factor 4 (PF4), beta-thromboglobulin (βTG) and monocyte chemotactic peptide-1 (MCP-1) were measured and compared in different stages of LC.RESULTS: Platelet activation with the increase in bothβTG serum concentration and elevation of plt population(CD62P and CD63 as well as MIF CD62P and CD63) is elevated as LC develops and thrombocytopenia rises. There is a positive correlation between medial intensityof fluorescence (MIF) CD62P and MIF CD63 in LC. We did not show any relationship between monocyte activation and pma level. SP-selectin concentration correlates positively with plt count and pma, and negatively with stage of plt activation and MIF CD62P and MIF CD63. There was no correlation between MCP-1 concentration andpit, monocyte activation as well as pma level in LC. CD16 monocytes and MIF CD16 populations are significantlyhigher in the end stage of LC. A positive correlation occurs between the value of CD11b monocyte population andMIF CD14 and MIF CD16 on monocytes in LC.CONCLUSION: Platelet and monocyte activation plays an important role in LC. Platelet activation stage does not influence monocyte activation and production of plt aggregates with monocytes in LC. With LC development, thrombocytopenia may be the result of plt consumption in platelet-monocyte aggregates. | Anatol Panasiuk Janusz Zak Edwina Kasprzycka Katarzyna Janicka Danuta Prokopowicz | 2005 | World Journal of Gastroenterology2005，11，18： | 11 |
| 3 | Liver fibrosis markers in alcoholic liver disease显示文摘Alcohol is one of the main factors of liver damage. The evaluation of the degree of liver fibrosis is of great value for therapeutic decision making in patients with alcoholic liver disease(ALD). Staging of liver fibrosis is essential to define prognosis and management of the disease. Liver biopsy is a gold standard as it has high sensitivity and specificity in fibrosis diagnostics. Taking into account the limitations of liver biopsy, there is an exigency to introduce non-invasive serum markers for fibrosis that would be able to replace liver biopsy. Ideal serum markers should be specific for the liver, easy to perform and independent to inflammation and fibrosis in other organs. Serum markers of hepatic fibrosis are divided into direct and indirect. Indirect markers reflect alterations in hepatic function, direct markers reflect extracellular matrix turnover. These markers should correlate with dynamic changes in fibrogenesis and fibrosis resolution. The assessment of the degree of liver fibrosis in alcoholic liver disease has diagnostic and prognostic implications, therefore noninvasive assessment of fibrosis remains important. There are only a few studies evaluating the diagnostic and prognostic values of noninvasive biomarkers of fibrosis in patients with ALD. Several noninvasive laboratory tests have beenused to assess liver fibrosis in patients with alcoholic liver disease, including the hyaluronic acid, FibroTest, FibrometerA, Hepascore, Forns and APRI indexes, FIB4, an algorithm combining Prothrombin index(PI), α-2 macroglobulin and hyaluronic acid. Among these tests, Fibrotest, FibrometerA and Hepascore demonstrated excellent diagnostic accuracy in identifying advanced fibrosis and cirrhosis, and additionally, Fibrotest was independently associated with survival. Therefore, the use of biomarkers may reduce the need for liver biopsy and permit an earlier treatment of alcoholic patients. | Lech Chrostek Anatol Panasiuk | 2014 | World Journal of Gastroenterology2014，20，25： | 6 |
| 4 | Soluble adhesion molecules ICAM-1,VCAM-1,P-selectin in children with Helicobacter pylori infection显示文摘AIM: To assess the sICAM-1, sVCAM-1, and sP-selectin levels in children withHelicobacter pylori(H pylori)infection and to evaluate their significance for the morphological changes found in gastric mucosa.METHODS: The study included 106 children: 59children (55.7%) with chronic gastritis and positive IgG against H pylori, 29 children (27.3%) after previous H pylori infection without the bacterium colonization but with positive IgG against H pylori, and 18 children (17%) with functional disorders of the gastrointestinal system but with normal IgG against H pylori. Endoscopic and histopathological evaluation of gastric mucosa was performed based on the Sydney System classification.The evaluation of sP-selectin, sIC AM-1, sVCAM-1 levels in the sera of children was carried out using ELISA test.RESULTS: The assessment of gastritis activity degrees indicated statistically significant values in the antrum and corpus (P<0.001) of children examined. Serum sVCAM-1 levels were higher in group with gastritis due to H pylori infection than in group without infection and differed statistically (P<0.05). Serum sVCAM-1 levels proved to be the highest among other adhesive molecules in infected children and decreased after eradication of H pylori. Serum sICAM-1 levels were similar in all examined groups. Serum sP-selectin levels were similar in children with and without H pylori infection.CONCLUSION: Assessment of adhesive molecules (sPselectin, sICAM-1, sVCAM-1) in the sera of children with active H pylori infection can show the participation of sVCAM-1 in the pathogenesis of gastric mucosal inflammation, sP-selectin and sICAM-1 concentrations in the sera of children with H pylori infection after eradication cannot reveal any significant differences as compared to healthy children. | Elzbieta Maciorkowska Maciej Kaczmarski Anatol Panasiuk Katarzyna Kondej-Muszynska Andrzej Kemonai | 2005 | World Journal of Gastroenterology2005，11，43： | 5 |
| 5 | Phagocytic and oxidative burst activity of neutrophils in the end stage of liver cirrhosis显示文摘AIM: To evaluate the phagocytic activity and neutrophil oxidative burst in liver cirrhosis.METHODS: In 45 patients with advanced postalcoholic liver cirrhosis (aged 45±14 years) and in 25 healthy volunteers (aged 38±5 years), the percentage of phagocytizing cells after in vitro incubation with E. coli (Phagotest Kit), phagocytic activity (mean intensity of fluorescence, MIF) and the percentage of neutrophil oxidative burst (Bursttest Kit), and the level of free oxygen radical production (MIF of Rodamine 123)were analyzed by flow cytometry. The levels of soluble sICAM-1, sVCAM-1, sP-selectin, sE-selectin, sL-selectin,and TNF-α were determined in blood serum.RESULTS: The percentage of E. coli phagocytizing neutrophils in liver cirrhosis patients was comparable to that in healthy subjects. MIF of neutrophil - ingested E. coli was higher in patients with liver cirrhosis. The oxidative burst in E. coli phagocytizing neutrophils generated less amount of active oxygen compounds in liver cirrhosis patients (MIF of R123:24.7±7.1 and 29.7±6.6 in healthy,P<0.01). Phorbol myristate acetate (PMA) - stimulated neutrophilsproduced less reactive oxidants in liver cirrhosis patients than in healthy subjects (MIF of R123: 42.7±14.6 vs 50.2±13.3, P<0.01). A negative correlation was observed between oxidative burst MIF of PMA-stimulated neutrophils and ALT and AST levels (r -0.35, P<0.05;r-0.4, P<0.03). sVCAM-1, sICAM-1, sE-selectin concentrations correlated negatively with the oxygen free radical production (MIF of R123) in neutrophils after PMA stimulation in liver cirrhosis patients (r-0.45, P<0.05;r-0.41, P<0.05; r-0.39, P<0.05, respectively).CONCLUSION: Neutrophil metabolic activity diminishes together with the intensification of liver failure. The metabolic potential of phagocytizing neutrophils is significantly lower in liver cirrhosis patients, which can be one of the causes of immune mechanism damage. The evaluation of oxygen metabolism of E. coli-stimulated neutrophils reveals that the amount of released oxygen metabolites is smaller in liver cirrhosis patients than in healthy subjects. | Anatol Panasiuk Jolanta Wysocka Elzbieta Maciorkowska Bozena Panasiuk Danuta Prokopowicz Janusz Zak Karol Radomski | 2005 | World Journal of Gastroenterology2005，11，48： | 4 |
| 6 | Signal transduction pathways in liver and the influence of hepatitis C virus infection on their activities显示文摘In liver,the most intensively studied transmembrane and intracellular signal transduction pathways are the Janus kinase signal transduction pathway,the mitogen-activated protein kinases signal transduction pathway,the transforming growth factor β signal transduction pathway,the tumor necrosis factor α signal transduction pathway and the recently discovered sphingolipid signal transduction pathway.All of them are activated by many different cytokines and growth factors.They regulate specific cell mechanisms such as hepatocytes proliferation,growth,differentiation,adhesion,apoptosis,and synthesis and degradation of the extracellular matrix.The replication cycle of hepatitis C virus(HCV) is intracellular and requires signal transduction to the nucleus to regulate transcription of its genes.Moreover,HCV itself,by its structural and non-structural proteins,could influence the activity of the second signal messengers.Thus,the inhibition of the transmembrane and intracellular signal transduction pathways could be a new therapeutic target in chronic hepatitis C treatment. | Magdalena M Dabrowska Anatol Panasiuk Robert Flisiak | 2009 | World Journal of Gastroenterology2009，15，18： | 3 |
| 7 | Expression of β2-integrin on leukocytes in liver cirrhosis显示文摘AIM: To analyze β2-integrin expression on blood leukocytes in liver cirrhosis. METHODS: In 40 patients with liver cirrhosis and 20 healthy individuals, the evaluation of expression of CD11a (LFA-1α), CD11b (Mac-1α), CD11c (αX) and CD49d (VLA-4α) on peripheral blood leukocytes was performed using flow cytometry. The analysis was carried out in groups of patients divided into B and C according to Child-Pugh’s classification. RESULTS: An increased CD11a, CD11b, CD11c and CD49d integrin expression was observed on peripheral blood leukocytes in liver cirrhosis. The integrin levels were elevated as the advancement of liver failure progressed. The highest expression of integrins occurred predominantly on monocytes. A slight expression of VLA-4 was found on lymphocytes and granulocytes and it increased together with liver failure. A positive correlation was noted between median intensity of fluorescence (MIF) expression on polymorphonuclear cells of CD11a and CD11c and CD49d (r = 0.42, P < 0.01; r = 053, P < 0.01, respectively) in liver cirrhosis stage C. However, no correlation was observed between integrin expression on leukocytes. The concentrations of sICAM-1, sVCAM-1, and TNFα, were significantly elevated in liver cirrhosis. CONCLUSION: β2-integrin expression on leukocytes increases in liver cirrhosis decompensated as the stage of liver failure increases, which is a result of permanent activation of leukocytes circulating through the inflamed liver environment. β2-integrin expression on circulating leukocytes can intensify liver cirrhosis. | Anatol Panasiuk Janusz ■ak Elzbieta Maciorkowska Bozena Panasiuk Danuta Prokopowicz | 2006 | World Journal of Gastroenterology2006，12，38： | 2 |
| 8 | Determination of α-amino nitrogen in pea protein hydrolyzates:a comparison of three analytical methods显示文摘 | Panasiuk R Amarowicz R Kostrya H | 1998 | Food Chemistry1998，62，3： | 1 |
| 9 | Reticulated platetets as a marker of megakaryopoiesis in liver cirrhosis: rela-tion to thrombopoietin and hepatocyte growth factor serum concentration 显示文摘 | PANASIUK A PROKOPOWICZ D ZAK J | 2004 | Hepato-Gastroenterology2004，51，58： | 1 |
| 10 | The evaluation of concentrations IL-5 and IL-6 in echinococcosis显示文摘 | Matowicka-Karna J Kemona H Panasiuk A | 2004 | Wiad Parazytol2004，50，： | 1 |
| 11 | Effect of free radicals on proliferation and metabolism of chondrocytes and onsensitivity of acellular monolayer toproteases显示文摘 | Panasiuk AF Laroshuk GV Mitrovich D | 1995 | Vopr Med Khim1995，41，： | 1 |
| 12 | Serum total and free sialic acid in patients with chronic liver disease显示文摘 | Cylwik B Chrostek L Panasiuk A | 2010 | Clin Chem Lab Med2010，48，： | 1 |
| 13 | Determination of a-amino nitrogen in pea protein hydrolysates: a comparison of three analytical methods显示文摘 | Panasiuk R Amarowicz R Kostyra H | 1998 | Food Chemistry1998，62，3： | 1 |
| 14 | Lipid-bound sialic acid (LSA) in liver diseases of different etiologies显示文摘 | Chrostek L Cylwik B Panasiuk A | 2011 | Ann Hepatol2011，10，： | 1 |
| 15 | MR and 1H MR Spectroscopy of the Brain in Patients with Liver Cirrhosis and Early Stages of Hepatic Eneephalopathy显示文摘 | Tarasow E Panasiuk A Siergiejczyk L | 2003 | Hepatogastroenterology2003，50，54： | 1 |
| 16 | Small interfering RNA knocks down the molecular target of alendronate, far- nesylpyrophosphate synthase, in osteoclast and osteoblast cultures显示文摘 | Wang Y Panasiuk A Grainger DW | 2011 | Mol Pharm2011，8，： | 1 |
| 17 | Increase in expression of monocytic tissue factor (CD142) with monocytes and blood platelet activation in liver cirrhosis 显示文摘 | Panasiuk A Zak J Panasiuk B | 2007 | Blood Coagul Fibrinolysis2007，18，8： | 1 |
| 18 | Plasma tissue inhibitor of met- alloproteinases - 1 and transforming growth factor beta 1 - - possible non - invasive biomarkers of hepatic fibrosis in patients with chronic B and C hepatitis 显示文摘 | Flisiak R Maxwell P Panasiuk A | 2002 | Hepatogastroenterology2002，49，47： | 1 |
| 19 | Ultra- sound diagnosis of acute appendicitis 显示文摘 | Semeniuk Iu S Panasiuk MH Ievkevych SA | 2013 | Klin Khir2013，2，2： | 1 |
| 20 | Autoimmune thrombocytopenia in chronic liverdisease显示文摘 | PANASIUK A ZAK J | 2001 | Pol Merkuriusz Lek2001，11，： | 1 |
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