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| 1 | Aging related methylation influences the gene expression of key control genes in colorectal cancer and adenoma显示文摘AIM To analyze colorectal carcinogenesis and age-related DNA methylation alterations of gene sequences associated with epigenetic clock CpG sites. METHODS In silico DNA methylation analysis of 353 epigenetic clock Cp G sites published by Steve Horvath was performed using methylation array data for a set of 123 colonic tissue samples [64 colorectal cancer(CRC), 42 adenoma, 17 normal; GEO accession number: GSE48684]. Among the differentially methylated agerelated genes, secreted frizzled related protein 1(SFRP1) promoter methylation was further investigated in colonic tissue from 8 healthy adults, 19 normal children, 20 adenoma and 8 CRC patients using bisulfite-specific PCR followed by methylation-specific high resolution melting(MS-HRM) analysis. m RNA expression of age-related 'epigenetic clock' genes was studied using Affymetrix HGU133 Plus2.0 whole transcriptome data of 153 colonic biopsy samples(49 healthy adult, 49 adenoma, 49 CRC, 6 healthy children)(GEO accession numbers: GSE37364, GSE10714, GSE4183, GSE37267). Whole promoter methylation analysis of genes showing inverse DNA methylationgene expression data was performed on 30 colonic samples using methyl capture sequencing.RESULTS Fifty-seven age-related Cp G sites including hypermethylated PPP1R16 B, SFRP1, SYNE1 and hypomethylated MGP, PIPOX were differentially methylated between CRC and normal tissues(P < 0.05, ?β≥ 10%). In the adenoma vs normal comparison, 70 CpG sites differed significantly, including hypermethylated DKK3, SDC2, SFRP1, SYNE1 and hypomethylated CEMIP, SPATA18(P < 0.05, ?β≥ 10%). In MS-HRM analysis, the SFRP1 promoter region was significantly hypermethylated in CRC(55.0% ± 8.4 %) and adenoma tissue samples(49.9% ± 18.1%) compared to normal adult(5.2% ± 2.7%) and young(2.2% ± 0.7%) colonic tissue(P < 0.0001). DNA methylation of SFRP1 promoter was slightly, but significantly increased in healthy adults compared to normal young samples(P < 0.02). This correlated with significantly increased SFRP1 m RNA levels in children compared to normal adult samples(P < 0.05). In CRC tissue the mR NA expression of 117 agerelated genes were changed, while in adenoma samples 102 genes showed differential expression compared with normal colonic tissue(P < 0.05, logF C > 0.5). The change of expression for several genes including SYNE1, CLEC3 B, LTBP3 and SFRP1, followed the same pattern in aging and carcinogenesis, though not for all genes(e.g., MGP). CONCLUSION Several age-related DNA methylation alterations can be observed during CRC development and progression affecting the m RNA expression of certain CRC- and adenoma-related key control genes. | Orsolya Galamb Alexandra Kalmár Barbara Kinga Barták árpád V Patai Katalin Leiszter Bálint Péterfia Barnabás Wichmann Gábor Valcz Gábor Veres Zsolt Tulassay Béla Molnár | 2016 | World Journal of Gastroenterology2016，22，47： | 7 |
| 2 | Serrated pathway:Alternative route to colorectal cancer显示文摘Serrated polyps have been an area of intense focus for gastroenterologists over the past several years. Contrary to what was thought before,a growing body of literature indicates that these polyps can be precursors of colorectal cancer(CRC).Most of these lesions, particularly those in the proximal colon,have so far been under-recognized and missed during colonoscopy,qualifying these lesions to be the main cause of interval cancers.It is estimated that 10%-20%of CRCs evolve through this alternative,serrated pathway, with a distinct genetic and epigenetic profile.Aberrant DNA methylation plays a central role in the development of this CRC subtype.This characteristic molecular background is reflected in a unique pathological and clinical manifestation different from cancers arising via the traditional pathway.In this review we would like to highlight morphological,molecular and clinical features of this emerging pathway that are essential for gastroenterologists and may influence their everyday practice. | rpád V Patai Béla Molnár Zsolt Tulassay Ferenc Sipos | 2013 | World Journal of Gastroenterology2013，19，5： | 4 |
| 3 | Phytochemical overview and medicinal importance of Coffea species from the past until now显示文摘Coffea(coffee) species are grown in almost all countries along the Equator. Many members of the genus have a large production history and an important role both in the global market and researches. Seeds(Coffeae semen) are successfully used in food, cosmetic, and pharmaceutical industries due to its caffeine and high polyphenol content. Nowadays, the three best-known coffee species are Arabic(Coffea arabica L.), Robusta(Coffea robusta L. Linden), and Liberian coffees(Coffea liberica Hier.). Even though, many records are available on coffee in scientific literature, wild coffee species like Bengal coffee(Coffea benghalensis Roxb. Ex Schult.) could offer many new opportunities and challenges for phytochemical and medical studies. In this comprehensive summary, we focused on the ethnomedicinal, phytochemical, and medical significance of coffee species up to the present. | éva Brigitta Patay Tímea Bencsik Nóra Papp | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，12： | 4 |
| 4 | Antioxidant potential,tannin and polyphenol contents of seed and pericarp of three Coffea species显示文摘Objective:To investigate the antioxidant activity,total phenolic and total tannin content of the pericarp and the seed of Coffea benghalensis(C.benghalensis) and Coffea liberica compared to Coffea arabica(C.arabica).Methods:The antioxidant potential,total tannin and polyphenol contents of the immature and mature seed and pericarp of C.benghalensis and Coffea liberica were quantified and compared to C.arabica.Enhanced chemiluminescence(ECL).2,2-diphenyl-1-picrylhydruzyl(DPPH).oxygen radical absorbance capacity,Folin-Ciocaltcau method and total tannin content assays were used.Results:Trolox equivalent(TE/g plant material) values obtained by ECL and DPPH methods showed loose correlation(r^2=0.587)while those measured by oxygen radical absorbance capacity assay were higher without correlation in each plant.A closer correlation was detected between the ECL method and the percentage antioxidant activity of the DPPH technique(r^2=0.610 7) in each species,however the immature pericarp of C.benghalensis showed much higher DPPH scavenging potential than was seen in the ECL assay.The immature pericarp of C.benghalensis expressed the highest tannin and polyphenol content,and a high polyphenol level was also detected in the immature seed of C.arabica.The immature pericarp of Bengal and Liberian coffees showed the largest amount of phenolic contents.Conclusions:The obtained data highlight the potential role of C.benghalensis as a new source of natural antioxidants and polyphenols compared to C.arabica. | va Brigitta Patay Nikolett Sali Tams Kszegi Rita Csepregi Viktória Lilla Blaz Tibor Sebastian Németh Tibor Németh Nóra Papp | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，4： | 3 |
| 5 | Spontaneous anaplasia in pilocytic astrocytoma of cerebellum显示文摘 | Lach B Al Shail E Patay Z | 2003 | Br J Neurosurg2003，17，3： | 1 |
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| 10 | Spontaneous anaplasia in pilo - eyrie astrocytoma of cerebellum 显示文摘 | Lach B AI Shail E Patay Z | 2003 | Br J Neurosurg2003，17，3： | 1 |
| 11 | Axillary lymph node metastasisfroman occult breast显示文摘 | Patai J Nemoto T Rosner D | 1981 | Cancer1981，47，12： | 1 |
| 12 | Diagnostic value of CA 19-9 and CEA in gastrointestinal pathology 显示文摘 | Patai A Heber S Dobronte Z | 1992 | Orv Hetil1992，133，13： | 1 |
| 13 | Giutaric aciduria type Ih observations in seven patients with neonatal- and late-onset disease显示文摘 | al-Essa MA Hashed MS Bakheet SM Patay ZJ Ozand PT | 2000 | Perinatol2000，20，2： | 1 |
| 14 | Congenital supratentorial meningeal arteriovenous malformation with hemangioma and massive arachnoid cell hyperplasia显示文摘 | Nabeel A Lach B Patay Z | 2005 | Childs Nerv Syst2005，21，11： | 1 |
| 15 | Clinico-radiologic characteristics of long-term survivors of diffuse intrinsic pontine glioma 显示文摘 | Jackson S Patay Z Howarth R | 2013 | J Neurooncol2013，114，3： | 1 |
| 16 | LC enantioseparation of 8-amino acids on a crown ether - based stationary phase显示文摘 | Berkecz R ll/sz I Patai Z | 2008 | Chromatographia2008，68，： | 1 |
| 17 | Diffusion-weighted MR Imaging in Leukodystrophies显示文摘 | Patay Z | 2005 | Eur Radiol2005，15，11： | 1 |
| 18 | Uterine leiomyosarcoma with osteoclast like giant cells and long standing systemic symptoms显示文摘 | Patai K Illyes G Varbiro S | 2006 | Gynecol Oncol2006，102，2： | 1 |
| 19 | Spontaneous anaplasia in pilo - eyrie astrocytoma of cerebellum 显示文摘 | Lach B AI Shail E Patay Z | 2003 | Br J Neurosurg2003，17，3： | 1 |
| 20 | Early detection of endometrial cancer by combined use of vaginal ultrasound and endometrial vacuum sampling显示文摘 | Patai K Szentmariay IF Jakab Z | 2002 | Int J Gynecol Cancer2002，12，3： | 1 |
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