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1Exercise as a prescription for patients with various diseases显示文摘A growing understanding of the benefits of exercise over the past few decades has prompted researchers to take an interest in the possibilities of exercise therapy.Because each sport has its own set of characteristics and physiological complications that tend to occur during exercise training,the effects and underlying mechanisms of exercise remain unclear.Thus,the first step in probing the effects of exercise on different diseases is the selection of an optimal exercise protocol.This review summarizes the latest exercise prescription treatments for 26 different diseases:musculoskeletal system diseases(low back pain,tendon injury,osteoporosis,osteoarthritis,and hip fracture),metabolic system diseases(obesity,type 2 diabetes,type 1 diabetes,and nonalcoholic fatty liver disease),cardio-cerebral vascular system diseases(coronary artery disease,stroke,and chronic heart failure),nervous system diseases(Parkinson’s disease,Huntington’s disease,Alzheimer’s disease,depression,and anxiety disorders),respiratory system diseases(chronic obstructive pulmonary disease,interstitial lung disease,and after lung transplantation),urinary system diseases(chronic kidney disease and after kidney transplantation),and cancers(breast cancer,colon cancer,prostate cancer,and lung cancer).Each exercise prescription is displayed in a corresponding table.The recommended type,intensity,and frequency of exercise prescriptions are summarized,and the effects of exercise therapy on the prevention and rehabilitation of different diseases are discussed.Xin Luan Xiangyang Tian Haixin Zhang Rui Huang Na Li Peijie Chen Ru Wang 2019Journal of Sport and Health Science2019,8,5:61
2Physical activity, physical fitness, and body mass index in the Chinese child and adolescent populations: An update from the 2016 Physical Activity and Fitness in China The Youth Study显示文摘In this special issue of the Journal of Sport and Health Science,analyses of the most recent national prevalence estimates from the 2016 Physical Activity and Fitness in China—The Youth Study(PAFCTYS)are presented.These include the prevalence of moderate-to-vigorous physical activity(MVPA)。Peijie Chen 2017Journal of Sport and Health Science2017,6,4:31
3The Cretaceous System in China显示文摘这篇论文在中国提供白垩纪地层学和 paleogeography 的一个轮廓,它基于从最近的研究获得的富有的数据。白垩纪存款在中国是普遍的。大多数阶层具有非海洋的起源,海洋的沉积仅仅在西藏, Xinjiang 的西方的 Tarim 盆,台湾和东方 Heilongjiang 的有限地区发生。所有存款富于石块并且抑制得好 biostratigraphically。不同区域的 stratigraphic 继任被说明,并且一般 stratigraphic 分割和关联被介绍了。海洋的存款在西藏的 Tethys, Xinjiang 的 Kashi-Hotan 区域,东方 Heilongjiang,西方的云南和台湾被描述;非海洋的存款从东北中国,东南中国,南部的内部中国,西南中国, Shaanxi-Gansu-Ningxia 区域,和西北的中国被构画出内部山区的盆。沉积外形和 paleogeography 是多样化的。在西藏,盆进化大部分与对欧亚的大陆的印度板的 subduction 和碰撞有关,并且在白垩纪显示出构造进化。有孔虫类是在西藏 Tethys 的一个主导的生物区系。Nonmarine 沉积包括使成杂色并且红床,忍受煤或咸忍受的地平线,和暴烈的岩石。这些存款包含多样、丰富的大陆人动物志和植物群,以及重要的煤和油资源。在中国的白垩纪地层学和 paleogeography 为地质的研究介绍了一个基础。WAN Xiaoqiao CHEN Peiji WEI Mingjian 2007Acta Geologica Sinica(English Edition)2007,81,6:15
4Coronavirus disease(COVID-19):The need to maintain regular physical activity while taking precautions显示文摘The on-going Coronavirus disease(COVID-19)outbreak in China has become the world's leading health headline and is causing major panic and public concerns.On January 30,2020,the World Health Organization(WHO)declared that the new coronavirus outbreak is a public health emergency of international concern.1 The virus has already had a direct impact on more than 10 million people in the city of Wuhan and has reached other parts of China as well,posing a health threat of unknown magnitude globally.As of February 8,2020,WHO reported 34,886 confirmed cases of COVID-19 globally,with 34,589 of them occurring in China(including 6101 severe cases and 723 deaths).A total of 288 other confirmed cases(with 1 death)have been reported in 24 countries,including Japan,Australia,Germany,and United States.2 However,these daily estimates are expected to rise even higher as reports from health authorities in China3 are gathered and near real-time updates of the Johns Hopkins virus dashboard are made 4 The outbreak has caused governments in various countries to take swift and protective measures.In China,these included putting cities on lockdown,5 implementing travel wamings/bans and cancellations,6 extending national holidays,and closing schools and postponing classes7.Peijie Chen Lijuan Mao George P.Nassis Peter Harmer Barbara E.Ainsworth Fuzhong Li 2020Journal of Sport and Health Science2020,9,2:15
5Liaoxiornis delicatus gen.et sp.nov.,the smallest Mesozoic bird显示文摘Recently, a very small and complete bird specimen was collected from the Confuciusornis-bearing Yixian Formation. This finding represents a new kind of Mesozoic bird, named Liaoxiornis delicatus. It is characterized by a short skull with teeth, a long neck and a very small sternum with only posterior process; the humeral head is not internally curved, and the hindlimbs are longer than the forelimbs. Liaoxiomis delicatus is not only much smaller than Confuciusornis, but also smaller than Cathayomis and other early Cretaceous birds. It is the smallest bird known from Mesozoic deposit. Liaoxiornis provides new evidence indicating that the early adaptive radiation of birds is complicated.HOU Lianhai and CHEN Peiji Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences , Beijing 100044, China Nanjing Institute of Geology and Paleontology, Chinese Academy of Sciences , China 1999Chinese Science Bulletin1999,44,9:13
6Meeting 24-h movement guidelines: Prevalence, correlates, and the relationships with overweight and obesity among Chinese children and adolescents显示文摘Background:Meeting 24-h movement guidelines by children and adolescents has been associated with improved indicators of health,although it has been under-studied in China.Hence,this study aimed to investigate the prevalence of meeting the 24-h movement guidelines,its correlates,and its relationships with body mass index in children and adolescents in China.Methods:Cross-sectional data from the 2017 Youth Study in China of 114,072 children and adolescents(mean age=13.75 years,49.18%boys)were used.Meeting 24-h movement guidelines(60 min of daily moderate-to-vigorous physical activity,2 h of daily leisure screen time,911 h and 810 h nightly sleep duration for 613-year-olds and 1417-year-olds,respectively)and height and weight of all participants were assessed.The prevalence of meeting the 24-h movement guidelines and World Health Organization weight status categories were determined.Generalized linear models were used to determine the correlates of meeting the 24-h movement guidelines and the relationships of meeting the 24-h movement guidelines with overweight(OW)and obesity(OB).Results:Only 5.12%of Chinese children and adolescents met the 24-h movement guidelines,and 22.44%were classified as OW/OB.Older children and adolescents were less likely to meet the 24-h movement guidelines.Parental education level and family income were positively related to meeting the 24-h movement guidelines.Children and adolescents meeting the 24-h movement guidelines showed lower odds ratios for OW/OB.Compared with participants meeting the 24-h movement guidelines,boys in 4th6th grades met none of the recommendations(OR=1.22,95%CI:1.061.40),met the screen time recommendation only(OR=1.13,95%CI:1.011.28),met the nightly sleep duration recommendation only(OR=1.14,95%CI:1.031.28),and had significantly higher odds ratios for OW/OB.Similar trends were observed for girls in 4th6th grades:meeting none of the guidelines(OR=1.35,95%CI:1.141.59),meeting sleep duration guidelines only(OR=1.23,95%CI:1.081.39),and meeting moderate-to-vigorous physical activity+nightly sleep duration guidelines(OR=1.24,95%CI:1.011.54).For girls in 7th9th grades,the following trend was observed:meeting none of the guidelines(OR=1.30,95%CI:1.011.67).Conclusion:Very few Chinese children and adolescents met the 24-h movement guidelines.Age(negatively correlated),parental education level,and family income(both positively correlated)were correlates of meeting the 24-h movement guidelines.Children and adolescents meeting the 24-h movement guidelines were more likely to have lower risks for OW/OB,especially in the youngest age group(Grades 46);and girls in the middle age group(Grades 79)were also more likely to have lower risks for OW/OB.Further research studies should explore additional correlates and determinants for meeting the 24-h movement guidelines.Also,future studies should use longitudinal or interventional designs to determine the relationships between meeting the 24-h movement guidelines and OW/OB and other health indicators,while taking sex and age differences into account.Si-Tong Chen Yang Liu Mark S.Tremblay Jin-Tao Hong Yan Tang Zhen-Bo Cao Jie Zhuang Zheng Zhu Xueping Wu Lijuan Wang Yujun Cai Peijie Chen 2021Journal of Sport and Health Science2021,10,3:12
7Environmental suitability evaluation for human settlements in an arid inland river basin: A case study of the Shiyang River Basin显示文摘学习采用斜坡,方面,陆地表面的消除度,陆地使用,植被索引,水文学和气候,当评估索引建立人的解决在 Shiyang 河盆为人的解决评估环境适用性的环境索引(HEI ) 模型。由例如空间覆盖分析,用 GIS 空间分析技术缓冲分析和密度分析,人的解决的环境适用性空间状况和空间模式被建立分析他们的空间分发。结果证明为在 Shiyang 河盆的人的解决的适用性的索引在 17.13 和 84.32 之间。一般来说,为人的解决的适用性从西南减少到东北。从一个区域模式看,合适的区域主要在 Minqin 绿洲, Wuwei 绿洲和 Changning 盆被散布,它为 2.59% 全部的区域是大约 1080.01 km2 和报道。更确切地说并且比较地合适的区域主要在 Tianzhu 的 Gulang, Yongchang 和北方的县附近被散布,它是大约 1100.30 km2。普通合适的区域主要在 Yongchang, Jinchuan 和 Minqin 县的大多数部分的县外面被散布,它是大约 23328.04 km2,为 56.08% 全部的区域的财务。不合适区域主要被散布在上游并且到河,是大约 9937.60 km2,的北方为 23.89% 全部的区域的财务。同时,最少的合适的区域在 Qilian 山附近被散布,它被雪和冷沙漠和谎言在在 Tengger 沙漠和 Badain Jaran 沙漠之间的交叉区域盖住。全部的区域是大约 6154.05 km2,为 14.79% 全部的区域的财务。为人的居住的合适的区域在在带子和批形式的河附近主要是分布式的,当其它被散布时。分发模式与居住空间模式相同。另外,在 HEI 之间的关系和另外的因素被分析了。在居住环境和人口之间有清楚的对数的关联,也就是说,在评估价值之间的关联系数和人口密度到达 0.851。也在居住环境和经济之间有积极关联,它到达在居住环境和 GDP 之间的 0.845 的评估价值。结果也证明环境从在 Shiyang 河盆容忍存在人口。人口的空间分发被严重环境问题深刻地影响,例如扩展沙漠,多山的地面和变化气候。表面水缺乏和慢经济的生长是为在 Shiyang 河盆的合适的人的解决的瓶颈。把这些问题与为新土地的建设和本地土地的利用计划相结合,一些居住区域应该被重定位改进居住环境。WEi Wei SHI Peiji ZHOU Junju FENG Haichun WANG Xufeng WANG Xueping 2013Journal of Geographical Sciences2013,23,2:11
8A novel function for the cellulose binding module of cellobiohydrolase I显示文摘A homogeneous cellulose-binding module(CBM)of cellobiohydrolase I(CBHI)from Trichoderma pseudokoningii S-38 was obtained by the limited proteolysis with papain and a series of chromatographs filtration.Analysis of FT-IR spectra demonstrated that the structural changes result from a weakening and splitting of the hydrogen bond network in cellulose by the action of CBMCBHI at 40℃for 24 h.The results of molecular dynamic simulations are consistent with the experimental conclusions, and provide a nanoscopic view of the mechanism that strong and medium H-bonds decreased dramatically when CBM was bound to the cellulose surface.The function of CBMCBHI is not only limited to locating intact CBHI in close proximity with cellulose fibrils,but also is involved in the structural disruption at the fibre surface.The present studies provided considerable evidence for the model of the intramolecular synergy between the catalytic domain and their CBMs.WANG LuShan 1,2 ,ZHANG YuZhong 1 &GAO PeiJi 1 1State Key Laboratory of Microbial Technology,Shandong University,Jinan 250100,China 2Beijing Laboratory of Nanoscale Physics&Devices,Chinese Academy of Sciences,Beijing 100080,China 2008Science China(Life Sciences)2008,51,7:10
9Tip-leakage flow loss reduction in a two-stage turbine using axisymmetric-casing contouring显示文摘In order to reduce the losses caused by tip-leakage flow, axisymmetric contouring is applied to the casing of a two-stage unshrouded high pressure turbine(HPT) of aero-engine in this paper. This investigation focuses on the effects of contoured axisymmetric-casing on the blade tipleakage flow. While the size of tip clearance remains the same as the original design, the rotor casing and the blade tip are obtained with the same contoured arc shape. Numerical calculation results show that a promotion of 0.14% to the overall efficiency is achieved. Detailed analysis indicates that it reduces the entropy generation rate caused by the complex vortex structure in the rotor tip region, especially in the tip-leakage vortex. The low velocity region in the leading edge(LE) part of the tip gap is enlarged and the pressure side/tip junction separation bubble extends much further away from the leading edge in the clearance. So the blocking effect of pressure side/tip junction separation bubble on clearance flow prevents more flow on the tip pressure side from leaking to the suction side, which results in weaker leakage vortex and less associated losses.Wei Zuojun Qiao Weiyang Shi Peijie Chen Pingping Zhao Lei 2014Chinese Journal of Aeronautics2014,27,5:9
10Quantitative determination of AI-2 quorum-sensing signal of bacteria using high performance liquid chromatography–tandem mass spectrometry显示文摘Autoinducer 2(AI-2), an important bioactive by-product of the Lux S-catalyzed S-ribosylhomocysteine cleavage reaction in the activated-methyl-cycle, has been suggested to serve as a universal intra- and inter-species signaling molecule. The development of reliable and sensitive methods for quantitative determination of AI-2 is highly desired.However, the chemical properties of AI-2 cause difficulty in its quantitative analysis.Herein, we report a high performance liquid chromatography-tandem mass spectrometric method that enables reproducible and sensitive measurement of AI-2 concentrations in complex matrixes. 4,5-Dimethylbenzene-1,2-diamine(DMBDM), an easy-to-obtain commercial reagent, was used for the derivatization treatment. The assay was linear in the concentration range of 1.0–1000 ng/m L(R^2= 0.999) and had a lower limit of quantification of0.58 ng/m L. The method exhibited several advantages, e.g., high selectivity, wide linear response range, and good sensitivity. Furthermore, the effectiveness of the method was further validated through measuring AI-2 concentrations in the cell-free culture supernatant from Escherichia coli wild type.Fang Xu Xiangning Song Peijie Cai Guoping Sheng Hanqing Yu 2017Journal of Environmental Sciences2017,29,2:8
11Physical activity and prevention of chronic disease in Chinese youth:A public health approach显示文摘Globally,physical activity(PA)has been recognized as one of the important lifestyle behaviors that brings major health benefits to,and affects the healthy growth of,school-aged children and adolescents.Fuzhong Li Lijuan Mao Peijie Chen 2019Journal of Sport and Health Science2019,8,6:8
12小鼠骨骼肌挫伤后基因表达的时间进程(英文)显示文摘Background:Though the mechanisms of skeletal muscle regeneration are deeply understood,those involved in muscle contusion,one of the most common muscle injuries in sports medicine clinics,are not.The objective of this study is to explore the mechanisms involved in muscle regeneration after contusion injury.Methods:In this study,a total of 72 mice were used.Eight of them were randomly chosen for the control group,while the rest were subjected to muscle contusion.Subsequently,their gastrocnemius muscles were harvested at different time points.The changes in muscle morphology were assessed by hematoxylin and eosin(HE) stain.In addition,the gene expression was analyzed by real-time polymerase chain reaction.Results:The data showed that the expression of many genes,i.e.,specific markers of immune cells and satellite cells,regulatory factors for muscle regeneration,cytokines,and chemokines,increased in the early stages of recovery,especially in the first 3 days.Furthermore,there were strict rules in the expression of these genes.However,almost all the genes returned to normal at 14 days post-injury.Conclusion:The sequence of immune cells invaded after muscle contusion was neutrophils,M1 macrophages and M2 macrophages.Some CC(CCL2,CCL3,and CCL4) and CXC(CXCL10) chemokines may be involved in the chemotaxis of these immune cells.HGF may be the primary factor to activate the satellite cells after muscle contusion.Moreover,2 weeks are needed to recover when acute contusion happens as used in this study.Weihua Xiao Yu Liu Beibei Luo Linlin Zhao Xiaoguang Liu Zhigang Zeng Peijie Chen 2016Journal of Sport and Health Science2016,5,1:8
13Associations between parental support for physical activity and moderate-to-vigorous physical activity among Chinese school children: A cross-sectional study显示文摘Purpose: The objective of this study was to examine the associations between parental support for physical activity(PA) and levels of moderate-to-vigorous physical activity(MVPA) among Chinese school children.Methods: Study participants were 81,857 school-aged children(aged 9–17 years; mean age = 13 years; 49.0% boys) and their parents or guardians who took part in the 2016 Physical Activity and Fitness in China—The Youth Study project. The independent variables included various forms of parental support for PA ascertained through a survey completed by parents or guardians. The dependent variable was the children's average MVPA in minutes per day by self-report. Structure equation modeling was used to examine the associations between parental support for PA and children's MVPA minutes.Results: The overall mean for MVPA participation time for the child participants was 48.82 ± 28.71 min/day. Significant associations between various forms of parental support and MVPA among children were found. Parental encouragement, accompanying, financial support, involvement,and role modeling for PA were positively associated with a high level of student MVPA participation. Parental PA knowledge-sharing was not associated with MVPA. The patterns of the associations were similar across sex, residence locale(i.e., urban and rural), and school grades(primary,junior middle, and junior high schools).Conclusion: Findings from this study suggest that, among Chinese school children, parental support for PA is important in promoting and facilitating children's participation in MVPA.Yang Liu Yajun Zhang Sitong Chen Jialin Zhang Zongchen Guo Peijie Chen 2017Journal of Sport and Health Science2017,6,4:7
14Remotely excited Raman optical activity using chiral plasmon propagation in Ag nanowires显示文摘We experimentally investigated remotely excited Raman optical activity(ROA)using propagating surface plasmons in chiral Ag nanowires.Using chiral fmoc-glycyl-glycine-OH(FGGO)molecules,we first studied the local surface plasmon-enhanced ROA.We found that the Raman intensity can be excited by left-and right-circularly polarized lights and that the circular intensity difference(CID)can be significantly enhanced.Second,by selecting vibrational modes with large Raman and ROA intensities that are not influenced by chemical enhancements,we studied remotely excited ROA imaging and the CID of FGGO molecules by propagating a plasmonic waveguide using Ag chiral nanostructures.When laser light was radiated on one of the Ag terminals,the measured CID of the FGG at the other terminal showed little change compared to the local excited CID.Meanwhile,when the laser light was radiated on the Ag nanowires(not on the terminals)and was coupled to the nearby nanoantenna,the CID of the ROA could be manipulated by altering the coupling angle between the Ag nanowires.To directly demonstrate the propagation of ROA along the nanowire and its remote detection,we also measured the remotely excited ROA spectra.Our experimental method has the potential to remotely determine the chirality of molecular structures and the absolute configuration or conformation of a chiral live cell.Mengtao Sun Zhenglong Zhang Peijie Wang Qiang Li Fengcai Ma Hongxing Xu 2013Light(Science & Applications)2013,2,1:7
15The biological effect of recombinant humanized collagen on damaged skin induced by UV-photoaging:An in vivo study显示文摘The application of medical devices to repair skin damage is clinically accepted and natural polymer enjoys an important role in this field,such as collagen or hyaluronic acid,etc.However,the biosafety and efficacy of these implants are still challenged.In this study,a skin damage animal model was prepared by UV-photoaging and recombinant humanized type Ⅲ collagen(rhCol Ⅲ)was applied as a bioactive material to implant in vivo to study its biological effect,comparing with saline and uncrosslinked hyaluronic acid(HA).Animal skin conditions were non-invasively and dynamically monitored during the 8 weeks experiment.Histological observation,specific gene expression and other molecular biological methods were applied by the end of the animal experiment.The results indicated that rhCol Ⅲ could alleviate the skin photoaging caused by UV radiation,including reduce the thickening of epidermis and dermis,increase the secretion of Collagen Ⅰ(Col Ⅰ)and Collagen Ⅲ(Col Ⅲ)and remodel of extracellular matrix(ECM).Although the cell-material interaction and mechanism need more investigation,the effect of rhCol Ⅲ on damaged skin was discussed from influence on cells,reconstruction of ECM,and stimulus of small biological molecules based on current results.In conclusion,our findings provided rigorous biosafety information of rhCol Ⅲ and approved its potential in skin repair and regeneration.Although enormous efforts still need to be made to achieve successful translation from bench to clinic,the recombinant humanized collagen showed superiorities from both safety and efficacy aspects.Jing Wang He Qiu Yang Xu Yongli Gao Peijie Tan Rui Zhao Zhanhong Liu Yajun Tang Xiangdong Zhu Chongyun Bao Hang Wang Hai Lin Xingdong Zhang 2022Bioactive Materials2022,7,5:7
16A novel approach for assessing the susceptibility of Escherichia coli to antibiotics显示文摘The dynamic growth process of Escherichia coli CVCC249 under different concentrations of antibiotics was analyzed. The results suggested that the main reason that definitive results cannot be obtained by antibiotic susceptibility testing (AST) is that the ratio of drug concentration to the population of bacteria and the combined effect of drug concentration and action time cannot be completely determined with the methods used. Based on the analysis of the growth process with a series of concentrations of gentamicin acting for a certain time, and according to the forward difference method, a novel method for AST was proposed. The net increase in turbidity of the bacterial population was used to eliminate the existing effects of resting cells, and then the recurrent coefficient for a growing sequence was used to characterize the effect of antibiotics on bacterial division, and the contour plot was used to display and analyze the combined effect of drug concentration and action time. The inhibition rate of the antibiotics can be characterized as the dynamic change in the composite function of the antibiotic concentration and action time, which indicated that the inhibition rate was dependent on the combined effect of time and concentration of antibiotics. The effectiveness of this new method has been verified with different kinds of antibiotics, such as enrofloxacin, levofloxacin, and ceftriaxone, having different antibacterial mechanisms.ZHANG HuaiQiang, ZHAO Yue, HE XiuLi & GAO PeiJi State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China 2010Science China(Life Sciences)2010,53,11:5
17A mechanically tuned Kerr comb in a dispersion-engineered silica microbubble resonator显示文摘This paper discusses the developement and investigation of a silica microbubble resonator(MBR) that is optimized to cancel mode dispersion with material dispersion, at a wavelength of approximately 1550 nm and maintain a quality factor of an optical mode as large as 5.4 × 10~7. Benefitting from the near-zero dispersion and high quality factor, a primary optical comb is generated in the MBR using cascaded four-wave mixing processes, which span over 300 nm with several tens of teeth. Furthermore, the frequency comb could be gradually tuned by mechanically stretching the MBR. This tunable Kerr comb has multiple potential applications in precision measurements and sensing applications, such as molecular spectroscopy and ranging.FangJie Shu PeiJi Zhang YanJun Qian ZhengYu Wang Shuai Wan Chang Ling Zou GuangCan Guo ChunHua Dong 2020Science China(Physics,Mechanics & Astronomy)2020,63,5:5
18Engineering Mesoporous Structure in Amorphous Carbon Boosts Potassium Storage with High Initial Coulombic Efficiency显示文摘Amorphous carbon shows great potential as an anode material for high-performance potassium-ion batteries;however,its abundant defects or micropores generally capture K ions,thus resulting in high irreversible capacity with low initial Coulombic efficiency(ICE)and limited practical application.Herein,pore engineering via a facile self-etching strategy is applied to achieve mesoporous carbon(meso-C)nanowires with interconnected framework.Abundant and evenly distributed mesopores could provide short K^+ pathways for its rapid diffusion.Compared to microporous carbon with highly disordered structure,the meso-C with Zn-catalyzed short-range ordered structure enables more K^+to reversibly intercalate into the graphitic layers.Consequently,the mesoC shows an increased capacity by ~100 mAh g^-1 at 0.1 A g^-1,and the capacity retention is 70.7% after 1000 cycles at 1 A g^-1.Multiple in/ex situ characterizations reveal the reversible structural changes during the charging/discharging process.Particularly,benefiting from the mesoporous structure with reduced specific surface area by 31.5 times and less defects,the meso-C generates less irreversible capacity with high ICE up to 76.7%,one of the best reported values so far.This work provides a new perspective that mesopores engineering can effectively accelerate K^+ diffusion and enhance K^+ adsorption/intercalation storage.Ruiting Guo Xiong Liu Bo Wen Fang Liu Jiashen Meng Peijie Wu Jinsong Wu Qi Li Liqiang Mai 2020Nano-Micro Letters2020,12,11:5
19A novel thermophilic endoglucanase from a mesophilic fungus Fusarium oxysporum显示文摘A novel thermophilic endoglucanase (EGt) was extracted from a mesophilic fungus (Fusarium oxysporum L19). We invoked conventional kinetic enzyme reactions using the sodium salt of carboxymethyl cellulose (CMC-Na) as substrate. EGt displayed optimal activity at 75℃ when kept running 30 min in the temperature range of 30―85℃. Thermal stability curve measured at 70℃ suggested that its half-life time is 15.1 min. The activity was enhanced in the presence of Co2+ or Mg2+ but inhibited by Pb2+ and Fe3+. Moreover, N-bromosuccinimide (NBS) modification resulted in a complete loss of EGt activity, suggesting that tryptophan residues may be involved in the enzyme active site. Amino acid composition analysis demonstrated that EGt contains more proline residues. EGt lacks activity towards p-nitro- phenyl cellobiose (pNPC). The N-terminal amino acid se- quence of EGt is SYRVPAANGFPNPDASQEKQ, and the gene of EGt was sequenced and analyzed. Extensive se- quence alignments failed to show any homology between EGt and any known endoglucanases. This is the first report addressing the thermal adaptation of a cellulolytic enzyme from the mesophilic fungus F. oxysporum. Maybe the ex- pression of multiple isoenzyme in an organism helps it adapt to complex living environments.LIU Shuyan DUAN Xinyuan LU Xuemei GAO Peiji 2006Chinese Science Bulletin2006,51,2:5
20Mechanism of cellobiose inhibition in cellulose hydrolysis by cellobiohydrolase显示文摘An experimental study of cellobiose inhibition in cellulose hydrolysis by synergism of cellobiohydrolyse I and endoglucanase I is presented. Cellobiose is the structural unit of cellulose molecules and also the main product in enzymatic hydrolysis of cellulose. It has been identified that cellobiose can strongly inhibit hydrolysis reaction of cellulase, whereas it has no effect on the adsorption of cellulase on cellulose surface. The experimental data of FT-IR spectra, fluorescence spectrum and circular dichroism suggested that cellobiose can be combined with tryptophan residue located near the active site of cellobiohydrolase and then form steric hindrance, which prevents cellulose molecule chains from diffusing into active site of cellulase. In addition, the molecular conformation of cellobiohydrolase changes after cellobiose binding, which also causes most of the non-productive adsorption. Under these conditions, microfibrils cannot be separated from cellulose chains, thus further hydrolysis of cellulose can hardly proceed.ZHAO Yue WU Bin YAN Baixu GAO Peiji 2004Science China(Life Sciences)2004,47,1:5
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